Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes

Introduction Mucosal immune activation, in the context of sexual transmission of HIV-1 infection, is crucial, as the increased presence of activated T cells enhance susceptibility to infection. In this regard, it has been proposed that immunomodulatory compounds capable of modulating immune activati...

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Autores:
Zapata Builes, Wildeman
González, Sandra Milena
Aguilar Jiménez, Wbeimar
Trujillo Gil, Edison
Ruey Chyi, Su
Rugeles López, María Teresa
T. Blake, Ball
Tipo de recurso:
Article of journal
Fecha de publicación:
2019
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
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oai:repository.ucc.edu.co:20.500.12494/15801
Acceso en línea:
https://doi.org/10.1371/journal.pone.0222878
https://hdl.handle.net/20.500.12494/15801
Palabra clave:
HIV-1
VitD
Calcitriol
HIV-1
Seronegative individuals
HIV-1
VitD
Calcitriol
HIV-1
Seronegative individuals
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openAccess
License
Atribución
id COOPER2_8b14f3fc2a3e4f7d9a84879f2bbe7000
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/15801
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
dc.title.spa.fl_str_mv Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes
title Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes
spellingShingle Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes
HIV-1
VitD
Calcitriol
HIV-1
Seronegative individuals
HIV-1
VitD
Calcitriol
HIV-1
Seronegative individuals
title_short Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes
title_full Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes
title_fullStr Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes
title_full_unstemmed Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes
title_sort Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes
dc.creator.fl_str_mv Zapata Builes, Wildeman
González, Sandra Milena
Aguilar Jiménez, Wbeimar
Trujillo Gil, Edison
Ruey Chyi, Su
Rugeles López, María Teresa
T. Blake, Ball
dc.contributor.author.none.fl_str_mv Zapata Builes, Wildeman
González, Sandra Milena
Aguilar Jiménez, Wbeimar
Trujillo Gil, Edison
Ruey Chyi, Su
Rugeles López, María Teresa
T. Blake, Ball
dc.subject.spa.fl_str_mv HIV-1
VitD
Calcitriol
HIV-1
Seronegative individuals
topic HIV-1
VitD
Calcitriol
HIV-1
Seronegative individuals
HIV-1
VitD
Calcitriol
HIV-1
Seronegative individuals
dc.subject.other.spa.fl_str_mv HIV-1
VitD
Calcitriol
HIV-1
Seronegative individuals
description Introduction Mucosal immune activation, in the context of sexual transmission of HIV-1 infection, is crucial, as the increased presence of activated T cells enhance susceptibility to infection. In this regard, it has been proposed that immunomodulatory compounds capable of modulating immune activation, such as Vitamin D (VitD) may reduce HIV-1 transmission and might be used as a safe and cost-effective strategy for prevention. Considering this, we examined the in vitro effect of the treatment of peripheral blood mononuclear cells (PBMCs) with the active form of VitD, calcitriol, on cellular activation, function and susceptibility of CD4+ T cells to HIV-1 infection. Methods We treated PBMCs from healthy HIV unexposed individuals (Co-HC) and frequently exposed, HIV-1 seronegative individuals (HESNs) from Colombia and from healthy nonexposed individuals from Canada (Ca-HC) with calcitriol and performed in vitro HIV-1 infection assays using X4- and R5-tropic HIV-1 strains respectively. In addition, we evaluated the activation and function of T cells and the expression of viral co-receptors, and select antiviral genes following calcitriol treatment. Results Calcitriol reduced the frequency of infected CD4+ T cells and the number of viral particles per cell, for both, X4- and R5-tropic viruses tested in the Co-HC and the Ca-HC, respectively, but not in HESNs. Furthermore, in the Co-HC, calcitriol reduced the frequency of polyclonally activated T cells expressing the activation markers HLA-DR and CD38, and those HLA-DR+CD38-, whereas increased the subpopulation HLA-DR-CD38+. Calcitriol treatment also decreased production of granzyme, IL-2 and MIP-1β by T cells and increased the transcriptional expression of the inhibitor of NF-kB and the antiviral genes cathelicidin (CAMP) and APOBEC3G in PBMCs from Co-HC. Conclusion Our in vitro findings suggest that VitD treatment could reduce HIV-1 transmission through a specific modulation of the activation levels and function of T cells, and the production of antiviral factors. In conclusion, VitD remains as an interesting potential strategy to prevent HIV- 1 transmission that should be further explored.
publishDate 2019
dc.date.accessioned.none.fl_str_mv 2019-12-17T17:08:03Z
dc.date.available.none.fl_str_mv 2019-12-17T17:08:03Z
dc.date.issued.none.fl_str_mv 2019-09-24
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
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dc.identifier.issn.spa.fl_str_mv 1932-6203
dc.identifier.uri.spa.fl_str_mv https://doi.org/10.1371/journal.pone.0222878
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/15801
dc.identifier.bibliographicCitation.spa.fl_str_mv Gonzalez S. M., Aguilar Jimenez W., Trujillo Gil E., Zapata W., Su R-C, Ball T. B., et al. (2019) Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes. PLoS ONE 14(9): e0222878. https://doi.org/10.1371/journal.pone.0222878. Recuperado de:
identifier_str_mv 1932-6203
Gonzalez S. M., Aguilar Jimenez W., Trujillo Gil E., Zapata W., Su R-C, Ball T. B., et al. (2019) Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes. PLoS ONE 14(9): e0222878. https://doi.org/10.1371/journal.pone.0222878. Recuperado de:
url https://doi.org/10.1371/journal.pone.0222878
https://hdl.handle.net/20.500.12494/15801
dc.relation.isversionof.spa.fl_str_mv https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0222878
dc.relation.ispartofjournal.spa.fl_str_mv PLOS ONE
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19. Aguilar-Jimenez W, Zapata W, Rugeles MT. Antiviral molecules correlate with vitamin D pathway genes and are associated with natural resistance to HIV-1 infection. Microbes Infect. 2016; 18(7– 8):510–6. https://doi.org/10.1016/j.micinf.2016.03.015 PMID: 27083474
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spelling Zapata Builes, WildemanGonzález, Sandra MilenaAguilar Jiménez, WbeimarTrujillo Gil, EdisonRuey Chyi, SuRugeles López, María TeresaT. Blake, Ball14(9)2019-12-17T17:08:03Z2019-12-17T17:08:03Z2019-09-241932-6203https://doi.org/10.1371/journal.pone.0222878https://hdl.handle.net/20.500.12494/15801Gonzalez S. M., Aguilar Jimenez W., Trujillo Gil E., Zapata W., Su R-C, Ball T. B., et al. (2019) Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes. PLoS ONE 14(9): e0222878. https://doi.org/10.1371/journal.pone.0222878. Recuperado de:Introduction Mucosal immune activation, in the context of sexual transmission of HIV-1 infection, is crucial, as the increased presence of activated T cells enhance susceptibility to infection. In this regard, it has been proposed that immunomodulatory compounds capable of modulating immune activation, such as Vitamin D (VitD) may reduce HIV-1 transmission and might be used as a safe and cost-effective strategy for prevention. Considering this, we examined the in vitro effect of the treatment of peripheral blood mononuclear cells (PBMCs) with the active form of VitD, calcitriol, on cellular activation, function and susceptibility of CD4+ T cells to HIV-1 infection. Methods We treated PBMCs from healthy HIV unexposed individuals (Co-HC) and frequently exposed, HIV-1 seronegative individuals (HESNs) from Colombia and from healthy nonexposed individuals from Canada (Ca-HC) with calcitriol and performed in vitro HIV-1 infection assays using X4- and R5-tropic HIV-1 strains respectively. In addition, we evaluated the activation and function of T cells and the expression of viral co-receptors, and select antiviral genes following calcitriol treatment. Results Calcitriol reduced the frequency of infected CD4+ T cells and the number of viral particles per cell, for both, X4- and R5-tropic viruses tested in the Co-HC and the Ca-HC, respectively, but not in HESNs. Furthermore, in the Co-HC, calcitriol reduced the frequency of polyclonally activated T cells expressing the activation markers HLA-DR and CD38, and those HLA-DR+CD38-, whereas increased the subpopulation HLA-DR-CD38+. Calcitriol treatment also decreased production of granzyme, IL-2 and MIP-1β by T cells and increased the transcriptional expression of the inhibitor of NF-kB and the antiviral genes cathelicidin (CAMP) and APOBEC3G in PBMCs from Co-HC. Conclusion Our in vitro findings suggest that VitD treatment could reduce HIV-1 transmission through a specific modulation of the activation levels and function of T cells, and the production of antiviral factors. In conclusion, VitD remains as an interesting potential strategy to prevent HIV- 1 transmission that should be further explored.https://scienti.minciencias.gov.co/cvlac/EnProdArticulo/query.do?cod_producto=73&cod_rh=0000157775https://orcid.org/0000-0002-7351-8738COL0112548wildeman.zapatab@campusucc.edu.comilena.gonzalez@udea.edu.coedisonetg@gmail.comrueyc.su@canada.caaguilar.wb@gmail.comblake.ball@umanitoba.camaria.rugeles@udea.edu.cohttps://scholar.google.com.co/citations?hl=en&user=VLZxl1UAAAAJ15Grupo Infettare, Facultad de Medicina, Universidad Cooperativa de Colombia, Medellin, ColombiaMedicinaMedellínhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0222878PLOS ONE1. Shapira-Nahor O, Kalinkovich A, Weisman Z, Greenberg Z, Nahmias J, Shapiro M, et al. Increased susceptibility to HIV-1 infection of peripheral blood mononuclear cells from chronically immune-activated individuals. AIDS. 1998; 12(13):1731–3. PMID: 97648022. 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Prog Neuropsychopharmacol Biol Psychiatry. 2016; 65: 242–251. https://doi.org/10.1016/j.pnpbp.2015.09.001 PMID: 26375241HIV-1VitDCalcitriolHIV-1Seronegative individualsHIV-1VitDCalcitriolHIV-1Seronegative individualsVitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytesArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribucióninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationORIGINALVitamin D treatment of peripheral blood UCC UdeA 2019.pdfVitamin D treatment of peripheral blood UCC UdeA 2019.pdfArtículoapplication/pdf1047762https://repository.ucc.edu.co/bitstreams/7022d55b-0083-4856-98b8-c0c0cc94cb2a/downloadabf6f434a8d4d8a115ba4e0c3da4bc7fMD51LICENSElicense.txtlicense.txttext/plain; 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