Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba
The safety and protective efficacy of the CPL-0015 Cuban isolate of canine parvovirus type 2 (CPV-2) were evaluated for its possible use as a vaccine strain candidate. The study included a total of 23 healthy Beagle dogs of both sexes, aged 84 days and without specific maternal antibodies against ca...
- Autores:
-
Zayas, Mayelin
Yen, Yenis
Naranjo, Gladys
Odio, Aníbal
Cala Delgado, Daniel Leonardo
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2022
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/52279
- Acceso en línea:
- http://dx.doi.org/10.4067/S0719-81322022000100023
https://hdl.handle.net/20.500.12494/52279
- Palabra clave:
- Parvovirus canino
Perros
Seguridad
Protección
Vacuna
- Rights
- openAccess
- License
- Atribución
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dc.title.none.fl_str_mv |
Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba |
title |
Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba |
spellingShingle |
Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba Parvovirus canino Perros Seguridad Protección Vacuna |
title_short |
Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba |
title_full |
Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba |
title_fullStr |
Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba |
title_full_unstemmed |
Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba |
title_sort |
Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba |
dc.creator.fl_str_mv |
Zayas, Mayelin Yen, Yenis Naranjo, Gladys Odio, Aníbal Cala Delgado, Daniel Leonardo |
dc.contributor.author.none.fl_str_mv |
Zayas, Mayelin Yen, Yenis Naranjo, Gladys Odio, Aníbal Cala Delgado, Daniel Leonardo |
dc.subject.none.fl_str_mv |
Parvovirus canino Perros Seguridad Protección Vacuna |
topic |
Parvovirus canino Perros Seguridad Protección Vacuna |
description |
The safety and protective efficacy of the CPL-0015 Cuban isolate of canine parvovirus type 2 (CPV-2) were evaluated for its possible use as a vaccine strain candidate. The study included a total of 23 healthy Beagle dogs of both sexes, aged 84 days and without specific maternal antibodies against canine parvovirus. Safety was analysed by comparing clinicopathological values, food consumption, body weight, rectal temperature and white blood cell counts for 14 consecutive days between control dogs (n=5) and dogs subcutaneously injected (n=10) with 2 mL (equivalent to two doses) of the CPL-0015 strain with an antigenic titer of 106.0 infectious dose50 in cell culture/mL. The protective effectiveness was determined by measuring and comparing anti-CPV-2 IgG levels and clinical signs during 56 experimental days between control dogs (n=2) and dogs inoculated (n=6) with double doses of 1 mL each, separated by a 21-day interval. All animals were challenged orally on day 35 with the virulent strain Cornell-780916 (105.0 infective dose50 in cell culture/mL). The results showed that the CPL-0015 strain did not negatively impact the physiological condition of the exposed animals. The inoculated and challenged animals showed not only significantly increased levels of anti-CPV-2 IgG (P<0.05) when compared to days 0, 35, and the control group animals but also had 100% survival without clinical signs of the disease, unlike the control group. It is concluded that CPL-0015 is safe and provides effective protection against homologous virulent strains |
publishDate |
2022 |
dc.date.available.none.fl_str_mv |
2022 2023-08-02T02:42:19Z |
dc.date.issued.none.fl_str_mv |
2022-03-15 |
dc.date.accessioned.none.fl_str_mv |
2023-08-02T02:42:19Z |
dc.type.none.fl_str_mv |
Artículos Científicos |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coarversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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publishedVersion |
dc.identifier.uri.none.fl_str_mv |
http://dx.doi.org/10.4067/S0719-81322022000100023 https://hdl.handle.net/20.500.12494/52279 |
dc.identifier.bibliographicCitation.none.fl_str_mv |
Zayas, Mayelin P., Yen, Yenis del T., Naranjo, Gladys P., Odio, Aníbal D., & Cala Delgado, Daniel L.. (2022). Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba. Austral journal of veterinary sciences, 54(1), 23-28. https://dx.doi.org/10.4067/S0719-81322022000100023 |
url |
http://dx.doi.org/10.4067/S0719-81322022000100023 https://hdl.handle.net/20.500.12494/52279 |
identifier_str_mv |
Zayas, Mayelin P., Yen, Yenis del T., Naranjo, Gladys P., Odio, Aníbal D., & Cala Delgado, Daniel L.. (2022). Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba. Austral journal of veterinary sciences, 54(1), 23-28. https://dx.doi.org/10.4067/S0719-81322022000100023 |
dc.relation.isversionof.none.fl_str_mv |
https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322022000100023&lng=es&nrm=iso&tlng=en |
dc.relation.ispartofjournal.none.fl_str_mv |
Austral journal of veterinary sciences |
dc.rights.license.none.fl_str_mv |
Atribución |
dc.rights.accessrights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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6 |
dc.coverage.temporal.none.fl_str_mv |
54 |
dc.publisher.none.fl_str_mv |
Grupo de Investigación en Ciencia Animal, Universidad Cooperativa de Colombia, Bucaramanga, Colombia Claudio Henríquez |
dc.publisher.program.none.fl_str_mv |
Medicina veterinaria y zootecnia |
dc.publisher.place.none.fl_str_mv |
Bucaramanga |
publisher.none.fl_str_mv |
Grupo de Investigación en Ciencia Animal, Universidad Cooperativa de Colombia, Bucaramanga, Colombia Claudio Henríquez |
institution |
Universidad Cooperativa de Colombia |
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Zayas, MayelinYen, YenisNaranjo, GladysOdio, AníbalCala Delgado, Daniel Leonardo542023-08-02T02:42:19Z2023-08-02T02:42:19Z20222022-03-15http://dx.doi.org/10.4067/S0719-81322022000100023https://hdl.handle.net/20.500.12494/52279Zayas, Mayelin P., Yen, Yenis del T., Naranjo, Gladys P., Odio, Aníbal D., & Cala Delgado, Daniel L.. (2022). Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba. Austral journal of veterinary sciences, 54(1), 23-28. https://dx.doi.org/10.4067/S0719-81322022000100023The safety and protective efficacy of the CPL-0015 Cuban isolate of canine parvovirus type 2 (CPV-2) were evaluated for its possible use as a vaccine strain candidate. The study included a total of 23 healthy Beagle dogs of both sexes, aged 84 days and without specific maternal antibodies against canine parvovirus. Safety was analysed by comparing clinicopathological values, food consumption, body weight, rectal temperature and white blood cell counts for 14 consecutive days between control dogs (n=5) and dogs subcutaneously injected (n=10) with 2 mL (equivalent to two doses) of the CPL-0015 strain with an antigenic titer of 106.0 infectious dose50 in cell culture/mL. The protective effectiveness was determined by measuring and comparing anti-CPV-2 IgG levels and clinical signs during 56 experimental days between control dogs (n=2) and dogs inoculated (n=6) with double doses of 1 mL each, separated by a 21-day interval. All animals were challenged orally on day 35 with the virulent strain Cornell-780916 (105.0 infective dose50 in cell culture/mL). The results showed that the CPL-0015 strain did not negatively impact the physiological condition of the exposed animals. The inoculated and challenged animals showed not only significantly increased levels of anti-CPV-2 IgG (P<0.05) when compared to days 0, 35, and the control group animals but also had 100% survival without clinical signs of the disease, unlike the control group. It is concluded that CPL-0015 is safe and provides effective protection against homologous virulent strainsThe safety and protective efficacy of the CPL-0015 Cuban isolate of canine parvovirus type 2 (CPV-2) were evaluated for its possible use as a vaccine strain candidate. The study included a total of 23 healthy Beagle dogs of both sexes, aged 84 days and without specific maternal antibodies against canine parvovirus. Safety was analysed by comparing clinicopathological values, food consumption, body weight, rectal temperature and white blood cell counts for 14 consecutive days between control dogs (n=5) and dogs subcutaneously injected (n=10) with 2 mL (equivalent to two doses) of the CPL-0015 strain with an antigenic titer of 106.0 infectious dose50 in cell culture/mL. The protective effectiveness was determined by measuring and comparing anti-CPV-2 IgG levels and clinical signs during 56 experimental days between control dogs (n=2) and dogs inoculated (n=6) with double doses of 1 mL each, separated by a 21-day interval. All animals were challenged orally on day 35 with the virulent strain Cornell-780916 (105.0 infective dose50 in cell culture/mL). The results showed that the CPL-0015 strain did not negatively impact the physiological condition of the exposed animals. The inoculated and challenged animals showed not only significantly increased levels of anti-CPV-2 IgG (P<0.05) when compared to days 0, 35, and the control group animals but also had 100% survival without clinical signs of the disease, unlike the control group. It is concluded that CPL-0015 is safe and provides effective protection against homologous virulent strainshttps://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000085088https://orcid.org/0000-0003-4639-5952https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000000695daniel.cala@campusucc.edu.co6Grupo de Investigación en Ciencia Animal, Universidad Cooperativa de Colombia, Bucaramanga, ColombiaClaudio HenríquezMedicina veterinaria y zootecniaBucaramangahttps://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322022000100023&lng=es&nrm=iso&tlng=enAustral journal of veterinary sciencesParvovirus caninoPerrosSeguridadProtecciónVacunaAssessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in CubaArtículos 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