Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba

The safety and protective efficacy of the CPL-0015 Cuban isolate of canine parvovirus type 2 (CPV-2) were evaluated for its possible use as a vaccine strain candidate. The study included a total of 23 healthy Beagle dogs of both sexes, aged 84 days and without specific maternal antibodies against ca...

Full description

Autores:
Zayas, Mayelin
Yen, Yenis
Naranjo, Gladys
Odio, Aníbal
Cala Delgado, Daniel Leonardo
Tipo de recurso:
Article of investigation
Fecha de publicación:
2022
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/52279
Acceso en línea:
http://dx.doi.org/10.4067/S0719-81322022000100023
https://hdl.handle.net/20.500.12494/52279
Palabra clave:
Parvovirus canino
Perros
Seguridad
Protección
Vacuna
Rights
openAccess
License
Atribución
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network_acronym_str COOPER2
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repository_id_str
dc.title.none.fl_str_mv Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba
title Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba
spellingShingle Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba
Parvovirus canino
Perros
Seguridad
Protección
Vacuna
title_short Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba
title_full Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba
title_fullStr Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba
title_full_unstemmed Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba
title_sort Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba
dc.creator.fl_str_mv Zayas, Mayelin
Yen, Yenis
Naranjo, Gladys
Odio, Aníbal
Cala Delgado, Daniel Leonardo
dc.contributor.author.none.fl_str_mv Zayas, Mayelin
Yen, Yenis
Naranjo, Gladys
Odio, Aníbal
Cala Delgado, Daniel Leonardo
dc.subject.none.fl_str_mv Parvovirus canino
Perros
Seguridad
Protección
Vacuna
topic Parvovirus canino
Perros
Seguridad
Protección
Vacuna
description The safety and protective efficacy of the CPL-0015 Cuban isolate of canine parvovirus type 2 (CPV-2) were evaluated for its possible use as a vaccine strain candidate. The study included a total of 23 healthy Beagle dogs of both sexes, aged 84 days and without specific maternal antibodies against canine parvovirus. Safety was analysed by comparing clinicopathological values, food consumption, body weight, rectal temperature and white blood cell counts for 14 consecutive days between control dogs (n=5) and dogs subcutaneously injected (n=10) with 2 mL (equivalent to two doses) of the CPL-0015 strain with an antigenic titer of 106.0 infectious dose50 in cell culture/mL. The protective effectiveness was determined by measuring and comparing anti-CPV-2 IgG levels and clinical signs during 56 experimental days between control dogs (n=2) and dogs inoculated (n=6) with double doses of 1 mL each, separated by a 21-day interval. All animals were challenged orally on day 35 with the virulent strain Cornell-780916 (105.0 infective dose50 in cell culture/mL). The results showed that the CPL-0015 strain did not negatively impact the physiological condition of the exposed animals. The inoculated and challenged animals showed not only significantly increased levels of anti-CPV-2 IgG (P<0.05) when compared to days 0, 35, and the control group animals but also had 100% survival without clinical signs of the disease, unlike the control group. It is concluded that CPL-0015 is safe and provides effective protection against homologous virulent strains
publishDate 2022
dc.date.available.none.fl_str_mv 2022
2023-08-02T02:42:19Z
dc.date.issued.none.fl_str_mv 2022-03-15
dc.date.accessioned.none.fl_str_mv 2023-08-02T02:42:19Z
dc.type.none.fl_str_mv Artículos Científicos
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dc.identifier.uri.none.fl_str_mv http://dx.doi.org/10.4067/S0719-81322022000100023
https://hdl.handle.net/20.500.12494/52279
dc.identifier.bibliographicCitation.none.fl_str_mv Zayas, Mayelin P., Yen, Yenis del T., Naranjo, Gladys P., Odio, Aníbal D., & Cala Delgado, Daniel L.. (2022). Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba. Austral journal of veterinary sciences, 54(1), 23-28. https://dx.doi.org/10.4067/S0719-81322022000100023
url http://dx.doi.org/10.4067/S0719-81322022000100023
https://hdl.handle.net/20.500.12494/52279
identifier_str_mv Zayas, Mayelin P., Yen, Yenis del T., Naranjo, Gladys P., Odio, Aníbal D., & Cala Delgado, Daniel L.. (2022). Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba. Austral journal of veterinary sciences, 54(1), 23-28. https://dx.doi.org/10.4067/S0719-81322022000100023
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dc.relation.ispartofjournal.none.fl_str_mv Austral journal of veterinary sciences
dc.rights.license.none.fl_str_mv Atribución
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
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rights_invalid_str_mv Atribución
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dc.format.extent.none.fl_str_mv 6
dc.coverage.temporal.none.fl_str_mv 54
dc.publisher.none.fl_str_mv Grupo de Investigación en Ciencia Animal, Universidad Cooperativa de Colombia, Bucaramanga, Colombia
Claudio Henríquez
dc.publisher.program.none.fl_str_mv Medicina veterinaria y zootecnia
dc.publisher.place.none.fl_str_mv Bucaramanga
publisher.none.fl_str_mv Grupo de Investigación en Ciencia Animal, Universidad Cooperativa de Colombia, Bucaramanga, Colombia
Claudio Henríquez
institution Universidad Cooperativa de Colombia
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spelling Zayas, MayelinYen, YenisNaranjo, GladysOdio, AníbalCala Delgado, Daniel Leonardo542023-08-02T02:42:19Z2023-08-02T02:42:19Z20222022-03-15http://dx.doi.org/10.4067/S0719-81322022000100023https://hdl.handle.net/20.500.12494/52279Zayas, Mayelin P., Yen, Yenis del T., Naranjo, Gladys P., Odio, Aníbal D., & Cala Delgado, Daniel L.. (2022). Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba. Austral journal of veterinary sciences, 54(1), 23-28. https://dx.doi.org/10.4067/S0719-81322022000100023The safety and protective efficacy of the CPL-0015 Cuban isolate of canine parvovirus type 2 (CPV-2) were evaluated for its possible use as a vaccine strain candidate. The study included a total of 23 healthy Beagle dogs of both sexes, aged 84 days and without specific maternal antibodies against canine parvovirus. Safety was analysed by comparing clinicopathological values, food consumption, body weight, rectal temperature and white blood cell counts for 14 consecutive days between control dogs (n=5) and dogs subcutaneously injected (n=10) with 2 mL (equivalent to two doses) of the CPL-0015 strain with an antigenic titer of 106.0 infectious dose50 in cell culture/mL. The protective effectiveness was determined by measuring and comparing anti-CPV-2 IgG levels and clinical signs during 56 experimental days between control dogs (n=2) and dogs inoculated (n=6) with double doses of 1 mL each, separated by a 21-day interval. All animals were challenged orally on day 35 with the virulent strain Cornell-780916 (105.0 infective dose50 in cell culture/mL). The results showed that the CPL-0015 strain did not negatively impact the physiological condition of the exposed animals. The inoculated and challenged animals showed not only significantly increased levels of anti-CPV-2 IgG (P<0.05) when compared to days 0, 35, and the control group animals but also had 100% survival without clinical signs of the disease, unlike the control group. It is concluded that CPL-0015 is safe and provides effective protection against homologous virulent strainsThe safety and protective efficacy of the CPL-0015 Cuban isolate of canine parvovirus type 2 (CPV-2) were evaluated for its possible use as a vaccine strain candidate. The study included a total of 23 healthy Beagle dogs of both sexes, aged 84 days and without specific maternal antibodies against canine parvovirus. Safety was analysed by comparing clinicopathological values, food consumption, body weight, rectal temperature and white blood cell counts for 14 consecutive days between control dogs (n=5) and dogs subcutaneously injected (n=10) with 2 mL (equivalent to two doses) of the CPL-0015 strain with an antigenic titer of 106.0 infectious dose50 in cell culture/mL. The protective effectiveness was determined by measuring and comparing anti-CPV-2 IgG levels and clinical signs during 56 experimental days between control dogs (n=2) and dogs inoculated (n=6) with double doses of 1 mL each, separated by a 21-day interval. All animals were challenged orally on day 35 with the virulent strain Cornell-780916 (105.0 infective dose50 in cell culture/mL). The results showed that the CPL-0015 strain did not negatively impact the physiological condition of the exposed animals. The inoculated and challenged animals showed not only significantly increased levels of anti-CPV-2 IgG (P<0.05) when compared to days 0, 35, and the control group animals but also had 100% survival without clinical signs of the disease, unlike the control group. It is concluded that CPL-0015 is safe and provides effective protection against homologous virulent strainshttps://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000085088https://orcid.org/0000-0003-4639-5952https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000000695daniel.cala@campusucc.edu.co6Grupo de Investigación en Ciencia Animal, Universidad Cooperativa de Colombia, Bucaramanga, ColombiaClaudio HenríquezMedicina veterinaria y zootecniaBucaramangahttps://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322022000100023&lng=es&nrm=iso&tlng=enAustral journal of veterinary sciencesParvovirus caninoPerrosSeguridadProtecciónVacunaAssessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in CubaArtículos 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