Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: A possible new CPV-2a is emerging?

Since its identification in 1978, Canine parvovirus type 2 (CPV-2) has been considered a pathogen of great importance in the canine population because it causes severe enteritis with high mortality rates in pups. CPV-2 is a virus belonging to the family Parvoviridae. Currently, there are three descr...

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Autores:
Duque García, Yeison H.
Echeverri Zuluaga, Manuela
Trejos-Suarez J.
Ruiz Saenz, Julian
Tipo de recurso:
Article of journal
Fecha de publicación:
2017
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/41615
Acceso en línea:
https://doi.org/10.1159/000353902
https://revistas.usantotomas.edu.co/index.php/diversitas/article/view/2513
https://hdl.handle.net/20.500.12494/41615
Palabra clave:
Antigenic variant
Hemorrhagic enteritis
Prevalence
Sequencing
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closedAccess
License
http://purl.org/coar/access_right/c_14cb
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oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/41615
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spelling Duque García, Yeison H.Echeverri Zuluaga, ManuelaTrejos-Suarez J.Ruiz Saenz, Julian2021-12-16T22:15:39Z2021-12-16T22:15:39Z2017https://doi.org/10.1159/000353902https://revistas.usantotomas.edu.co/index.php/diversitas/article/view/251303781135https://hdl.handle.net/20.500.12494/41615Duque Y,Echeverri M,Trejos J,Ruiz J. Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: A possible new CPV-2a is emerging?. Vet Microbiol. 2017. 201. p. 56-61. .Since its identification in 1978, Canine parvovirus type 2 (CPV-2) has been considered a pathogen of great importance in the canine population because it causes severe enteritis with high mortality rates in pups. CPV-2 is a virus belonging to the family Parvoviridae. Currently, there are three described antigenic variants (CPV-2a, CPV-2b, and CPV-2c). CPV-2c is an emerging virus that is seen as a global health hazard. The objective of this work was to confirm the presence of CPV-2 in dogs with acute gastroenteritis compatible with parvovirus and to molecularly characterize the antigenic variants circulating in two regions of Colombia. An analytical cross-sectional study was conducted with fecal samples collected from 71 dogs showing signs of acute diarrhea. The samples were processed and polymerase chain reaction (PCR), restriction fragment length polymorphism analysis (RFLP), sequencing and phylogenetic analysis was performed to detect and characterize CPV. A total of 70.42% of the individuals were confirmed positive for CPV-2. Statistically differences were found in the presentation of CPV-2 between the evaluated regions. Phylogenetic analyses confirmed the presence of the antigenic variants CPV-2a/2b. Moreover, we found the presence of two conserved substitutions Asn428Asp and Ala514Ser in the VP2 protein suggesting the presence of a possible new CPV-2a variant circulating in Colombia. This study demonstrates the importance of the CPV 2a/2b in the region and highlights the importance of performing molecular studies for the early detection of new antigenic variants of CPV-2. © 2017 Elsevier B.V.0000-0002-1447-1458julian.ruizs@campusucc.edu.co61-56Elsevier B.V.Antigenic variantHemorrhagic enteritisPrevalenceSequencingPrevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: A possible new CPV-2a is emerging?Artículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionVeterinary Microbiologyinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/41615oai:repository.ucc.edu.co:20.500.12494/416152024-08-20 16:19:05.761metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: A possible new CPV-2a is emerging?
title Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: A possible new CPV-2a is emerging?
spellingShingle Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: A possible new CPV-2a is emerging?
Antigenic variant
Hemorrhagic enteritis
Prevalence
Sequencing
title_short Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: A possible new CPV-2a is emerging?
title_full Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: A possible new CPV-2a is emerging?
title_fullStr Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: A possible new CPV-2a is emerging?
title_full_unstemmed Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: A possible new CPV-2a is emerging?
title_sort Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: A possible new CPV-2a is emerging?
dc.creator.fl_str_mv Duque García, Yeison H.
Echeverri Zuluaga, Manuela
Trejos-Suarez J.
Ruiz Saenz, Julian
dc.contributor.author.none.fl_str_mv Duque García, Yeison H.
Echeverri Zuluaga, Manuela
Trejos-Suarez J.
Ruiz Saenz, Julian
dc.subject.spa.fl_str_mv Antigenic variant
Hemorrhagic enteritis
Prevalence
Sequencing
topic Antigenic variant
Hemorrhagic enteritis
Prevalence
Sequencing
description Since its identification in 1978, Canine parvovirus type 2 (CPV-2) has been considered a pathogen of great importance in the canine population because it causes severe enteritis with high mortality rates in pups. CPV-2 is a virus belonging to the family Parvoviridae. Currently, there are three described antigenic variants (CPV-2a, CPV-2b, and CPV-2c). CPV-2c is an emerging virus that is seen as a global health hazard. The objective of this work was to confirm the presence of CPV-2 in dogs with acute gastroenteritis compatible with parvovirus and to molecularly characterize the antigenic variants circulating in two regions of Colombia. An analytical cross-sectional study was conducted with fecal samples collected from 71 dogs showing signs of acute diarrhea. The samples were processed and polymerase chain reaction (PCR), restriction fragment length polymorphism analysis (RFLP), sequencing and phylogenetic analysis was performed to detect and characterize CPV. A total of 70.42% of the individuals were confirmed positive for CPV-2. Statistically differences were found in the presentation of CPV-2 between the evaluated regions. Phylogenetic analyses confirmed the presence of the antigenic variants CPV-2a/2b. Moreover, we found the presence of two conserved substitutions Asn428Asp and Ala514Ser in the VP2 protein suggesting the presence of a possible new CPV-2a variant circulating in Colombia. This study demonstrates the importance of the CPV 2a/2b in the region and highlights the importance of performing molecular studies for the early detection of new antigenic variants of CPV-2. © 2017 Elsevier B.V.
publishDate 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2021-12-16T22:15:39Z
dc.date.available.none.fl_str_mv 2021-12-16T22:15:39Z
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.none.fl_str_mv https://doi.org/10.1159/000353902
https://revistas.usantotomas.edu.co/index.php/diversitas/article/view/2513
dc.identifier.issn.spa.fl_str_mv 03781135
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/41615
dc.identifier.bibliographicCitation.spa.fl_str_mv Duque Y,Echeverri M,Trejos J,Ruiz J. Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: A possible new CPV-2a is emerging?. Vet Microbiol. 2017. 201. p. 56-61. .
url https://doi.org/10.1159/000353902
https://revistas.usantotomas.edu.co/index.php/diversitas/article/view/2513
https://hdl.handle.net/20.500.12494/41615
identifier_str_mv 03781135
Duque Y,Echeverri M,Trejos J,Ruiz J. Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: A possible new CPV-2a is emerging?. Vet Microbiol. 2017. 201. p. 56-61. .
dc.relation.ispartofjournal.spa.fl_str_mv Veterinary Microbiology
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/closedAccess
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eu_rights_str_mv closedAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_14cb
dc.format.extent.spa.fl_str_mv 61-56
dc.publisher.spa.fl_str_mv Elsevier B.V.
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
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