Prevalence and molecular typing of rotavirus in children with acute diarrhoea in Northeastern Colombia.
After the introduction of the rotavirus vaccine, the number of rotavirus-associated deaths and the predicted annual rotavirus detection rate had slightly declined worldwide. Taking in account that in Colombia, Rotarix vaccine was introduced in 2009, the purpose of this study was to evaluate the pres...
- Autores:
-
Ruiz Sáenz, Julián
Martínez Gutiérrez, Marlen
Arcila Quiceno, Víctor Hernán
Trejos Suárez, Juanita
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2019
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/15181
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/15181
- Palabra clave:
- Acute diarrhoea
Colombia
Molecular characterization
Post-vaccination
Rotavirus
- Rights
- openAccess
- License
- Atribución
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Prevalence and molecular typing of rotavirus in children with acute diarrhoea in Northeastern Colombia. |
| title |
Prevalence and molecular typing of rotavirus in children with acute diarrhoea in Northeastern Colombia. |
| spellingShingle |
Prevalence and molecular typing of rotavirus in children with acute diarrhoea in Northeastern Colombia. Acute diarrhoea Colombia Molecular characterization Post-vaccination Rotavirus |
| title_short |
Prevalence and molecular typing of rotavirus in children with acute diarrhoea in Northeastern Colombia. |
| title_full |
Prevalence and molecular typing of rotavirus in children with acute diarrhoea in Northeastern Colombia. |
| title_fullStr |
Prevalence and molecular typing of rotavirus in children with acute diarrhoea in Northeastern Colombia. |
| title_full_unstemmed |
Prevalence and molecular typing of rotavirus in children with acute diarrhoea in Northeastern Colombia. |
| title_sort |
Prevalence and molecular typing of rotavirus in children with acute diarrhoea in Northeastern Colombia. |
| dc.creator.fl_str_mv |
Ruiz Sáenz, Julián Martínez Gutiérrez, Marlen Arcila Quiceno, Víctor Hernán Trejos Suárez, Juanita |
| dc.contributor.author.none.fl_str_mv |
Ruiz Sáenz, Julián Martínez Gutiérrez, Marlen Arcila Quiceno, Víctor Hernán Trejos Suárez, Juanita |
| dc.subject.spa.fl_str_mv |
Acute diarrhoea Colombia Molecular characterization Post-vaccination Rotavirus |
| topic |
Acute diarrhoea Colombia Molecular characterization Post-vaccination Rotavirus |
| description |
After the introduction of the rotavirus vaccine, the number of rotavirus-associated deaths and the predicted annual rotavirus detection rate had slightly declined worldwide. Taking in account that in Colombia, Rotarix vaccine was introduced in 2009, the purpose of this study was to evaluate the presence of rotavirus A in children under five years who were treated for acute diarrhoea in Bucaramanga, Colombia and, moreover, to determine the genotypes of rotavirus present in those children. We performed an analytical cross-sectional study of rotavirus A in faecal samples from children up to five years of age. Stool samples were screened for rotavirus A using a lateral-flow immunochromatographic assay and confirmed using a VP6 sandwich ELISA. Genotyping of rotavirus A-positive samples was performed by PCR and sequencing of VP7 and VP4 genes. The overall prevalence of rotavirus was 30.53% (95% confidence interval [CI] 21.2 - 39.7). Most of the children with rotavirus (86.2%) had received two doses of the rotavirus vaccine. G3 strains accounted for the vast majority of cases (82.8%), followed by G12 strains (13.8%) and G3/G9 coinfections (3.4%). Among the P genotypes, P[8] was the most prevalent (69%), followed by P[9] (31%). The most common G[P] genotype combination was G3P[8], followed by G3P[9]. The main finding in this study was that rotavirus, in a Colombian region, is still an important pathogen in children under five years old, previously vaccinated. The results showed that different factors, such as kindergarten attendance, could explain the epidemiology and transmission of rotavirus in Bucaramanga. |
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2019 |
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2019-11-22T13:23:27Z |
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2019-11-22T13:23:27Z |
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2019-07-01 |
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Artículo |
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1678-9946 |
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doi: 10.1590/S1678-9946201961034. |
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https://hdl.handle.net/20.500.12494/15181 |
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Martinez-Gutierrez M, Arcila-Quiceno V, Trejos-Suarez J, Ruiz-Saenz J. (2019) Prevalence and molecular typing of rotavirus in children with acute diarrhoea in Northeastern Colombia. Rev Inst Med Trop Sao Paulo. 2019 Jul 1;61:e34. doi: 10.1590/S1678-9946201961034. |
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1678-9946 doi: 10.1590/S1678-9946201961034. Martinez-Gutierrez M, Arcila-Quiceno V, Trejos-Suarez J, Ruiz-Saenz J. (2019) Prevalence and molecular typing of rotavirus in children with acute diarrhoea in Northeastern Colombia. Rev Inst Med Trop Sao Paulo. 2019 Jul 1;61:e34. doi: 10.1590/S1678-9946201961034. |
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https://hdl.handle.net/20.500.12494/15181 |
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Rev Inst Med Trop Sao Paulo |
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1. Nguyen TV, Le Van P, Le Huy C, Weintraub A. Diarrhea caused by rotavirus in children less than 5 years of age in Hanoi, Vietnam. J Clin Microbiol. 2004;42:5745-50. 2. Mokomane M, Kasvosve I, Melo E, Pernica JM, Goldfarb DM. The global problem of childhood diarrhoeal diseases: emerging strategies in prevention and management. Ther Adv Infect Dis. 2018;5:29-43. 3. GBD Diarrhoeal Diseases Collaborators. Estimates of global, regional, and national morbidity, mortality, and aetiologies of diarrhoeal diseases: a systematic analysis for the Global Burden of Disease Study 2015. Lancet Infect Dis. 2017;17:909-48. 4. Crawford SE, Ramani S, Tate JE, Parashar UD, Svensson L, Hagbom M, et al. Rotavirus infection. Nat Rev Dis Primers. 2017;3:17083. 5. Parashar UD, Gibson CJ, Bresse J, Glass RI. Rotavirus and severe childhood diarrhea. Emerg Infect Dis. 2006;12:304-6. 6. World Health Organization. Meeting of the immunization Strategic Advisory Group of Experts, April 2009: conclusions and recommendations. Wkly Epidemiol Rec. 2009;84:220-36. 7. Tate JE, Burton AH, Boschi-Pinto C, Parashar UD, World Health Organization-Coordinated Global Rotavirus Surveillance Network. Global, regional, and national estimates of rotavirus mortality in children <5 years of age, 2000-2013. Clin Infect Dis. 2016;62 Suppl 2:S96-105. 8. Troeger C, Khalil IA, Rao PC, Cao S, Blacker BF, Ahmed T, et al. Rotavirus vaccination and the global burden of rotavirus diarrhea among children younger than 5 years. JAMA Pediatr. 2018;172:958-65. 9. Bucardo F, Nordgren J. Impact of vaccination on the molecular epidemiology and evolution of group A rotaviruses in Latin America and factors affecting vaccine efficacy. Infect Genet Evol. 2015;34:106-13. 10. Parashar UD, Johnson H, Steele AD, Tate JE. Health impact of rotavirus vaccination in developing countries: progress and way forward. Clin Infect Dis. 2016;62 Suppl 2:S91-5. 11. Cáceres DC, Peláez D, Sierra N, Estrada E, Sánchez L. La carga de la enfermedad por rotavirus en niños menores de cinco años, Colombia, 2004. Rev Panam Salud Publica. 2006;20:9-21. 12. Uribe Yepes MA, Rodríguez Villamizar LA, Gómez González YA, Olaya Gamboa LE, Rodríguez Santamaría SM. Aislamientos de patógenos comunes asociados con enfermedad diarreica aguda en menores de cinco años, Bucaramanga, Colombia. MedUNAB. 2009;12:72-9. 13. World Health Organization. Manual of rotavirus detection and characterization methods. Geneva: WHO; 2009. 14. Gouvea V, Glass RI, Woods P, Taniguchi K, Clark HF, Forrester B, et al. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol. 1990;28:276-82. |
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Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, Bucaramanga |
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Medicina veterinaria y zootecnia |
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Ruiz Sáenz, JuliánMartínez Gutiérrez, MarlenArcila Quiceno, Víctor HernánTrejos Suárez, Juanita612019-11-22T13:23:27Z2019-11-22T13:23:27Z2019-07-011678-9946doi: 10.1590/S1678-9946201961034.https://hdl.handle.net/20.500.12494/15181Martinez-Gutierrez M, Arcila-Quiceno V, Trejos-Suarez J, Ruiz-Saenz J. (2019) Prevalence and molecular typing of rotavirus in children with acute diarrhoea in Northeastern Colombia. Rev Inst Med Trop Sao Paulo. 2019 Jul 1;61:e34. doi: 10.1590/S1678-9946201961034.After the introduction of the rotavirus vaccine, the number of rotavirus-associated deaths and the predicted annual rotavirus detection rate had slightly declined worldwide. Taking in account that in Colombia, Rotarix vaccine was introduced in 2009, the purpose of this study was to evaluate the presence of rotavirus A in children under five years who were treated for acute diarrhoea in Bucaramanga, Colombia and, moreover, to determine the genotypes of rotavirus present in those children. We performed an analytical cross-sectional study of rotavirus A in faecal samples from children up to five years of age. Stool samples were screened for rotavirus A using a lateral-flow immunochromatographic assay and confirmed using a VP6 sandwich ELISA. Genotyping of rotavirus A-positive samples was performed by PCR and sequencing of VP7 and VP4 genes. The overall prevalence of rotavirus was 30.53% (95% confidence interval [CI] 21.2 - 39.7). Most of the children with rotavirus (86.2%) had received two doses of the rotavirus vaccine. G3 strains accounted for the vast majority of cases (82.8%), followed by G12 strains (13.8%) and G3/G9 coinfections (3.4%). Among the P genotypes, P[8] was the most prevalent (69%), followed by P[9] (31%). The most common G[P] genotype combination was G3P[8], followed by G3P[9]. The main finding in this study was that rotavirus, in a Colombian region, is still an important pathogen in children under five years old, previously vaccinated. The results showed that different factors, such as kindergarten attendance, could explain the epidemiology and transmission of rotavirus in Bucaramanga.http://scienti.colciencias.gov.co:8081/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000153095https://scienti.colciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000213748https://orcid.org/0000-0002-1447-1458https://orcid.org/0000-0002-9429-0058julian.ruizs@campusucc.edu.coMarlen.martinezg@campucucc.edu.covictor.arcila@ucc.edu.cohttps://scholar.google.com/citations?user=o3Y7mZwAAAAJ&hl=eshttps://scholar.google.es/citations?user=flSrsSIAAAAJ&hl=esUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, BucaramangaMedicina veterinaria y zootecniaBucaramangahttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31269110/Rev Inst Med Trop Sao Paulo1. Nguyen TV, Le Van P, Le Huy C, Weintraub A. Diarrhea caused by rotavirus in children less than 5 years of age in Hanoi, Vietnam. J Clin Microbiol. 2004;42:5745-50.2. Mokomane M, Kasvosve I, Melo E, Pernica JM, Goldfarb DM. The global problem of childhood diarrhoeal diseases: emerging strategies in prevention and management. Ther Adv Infect Dis. 2018;5:29-43.3. GBD Diarrhoeal Diseases Collaborators. Estimates of global, regional, and national morbidity, mortality, and aetiologies of diarrhoeal diseases: a systematic analysis for the Global Burden of Disease Study 2015. Lancet Infect Dis. 2017;17:909-48.4. Crawford SE, Ramani S, Tate JE, Parashar UD, Svensson L, Hagbom M, et al. Rotavirus infection. Nat Rev Dis Primers. 2017;3:17083.5. Parashar UD, Gibson CJ, Bresse J, Glass RI. Rotavirus and severe childhood diarrhea. Emerg Infect Dis. 2006;12:304-6.6. World Health Organization. Meeting of the immunization Strategic Advisory Group of Experts, April 2009: conclusions and recommendations. Wkly Epidemiol Rec. 2009;84:220-36.7. Tate JE, Burton AH, Boschi-Pinto C, Parashar UD, World Health Organization-Coordinated Global Rotavirus Surveillance Network. Global, regional, and national estimates of rotavirus mortality in children <5 years of age, 2000-2013. Clin Infect Dis. 2016;62 Suppl 2:S96-105.8. Troeger C, Khalil IA, Rao PC, Cao S, Blacker BF, Ahmed T, et al. Rotavirus vaccination and the global burden of rotavirus diarrhea among children younger than 5 years. JAMA Pediatr. 2018;172:958-65.9. Bucardo F, Nordgren J. Impact of vaccination on the molecular epidemiology and evolution of group A rotaviruses in Latin America and factors affecting vaccine efficacy. Infect Genet Evol. 2015;34:106-13.10. Parashar UD, Johnson H, Steele AD, Tate JE. Health impact of rotavirus vaccination in developing countries: progress and way forward. Clin Infect Dis. 2016;62 Suppl 2:S91-5.11. Cáceres DC, Peláez D, Sierra N, Estrada E, Sánchez L. La carga de la enfermedad por rotavirus en niños menores de cinco años, Colombia, 2004. Rev Panam Salud Publica. 2006;20:9-21.12. Uribe Yepes MA, Rodríguez Villamizar LA, Gómez González YA, Olaya Gamboa LE, Rodríguez Santamaría SM. Aislamientos de patógenos comunes asociados con enfermedad diarreica aguda en menores de cinco años, Bucaramanga, Colombia. MedUNAB. 2009;12:72-9.13. World Health Organization. Manual of rotavirus detection and characterization methods. Geneva: WHO; 2009.14. Gouvea V, Glass RI, Woods P, Taniguchi K, Clark HF, Forrester B, et al. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol. 1990;28:276-82.Acute diarrhoeaColombiaMolecular characterizationPost-vaccinationRotavirusPrevalence and molecular typing of rotavirus in children with acute diarrhoea in Northeastern Colombia.Artículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribucióninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-84334https://repository.ucc.edu.co/bitstreams/2756e005-5747-4b7a-a8b7-b269ffbe1444/download3bce4f7ab09dfc588f126e1e36e98a45MD54ORIGINALMartinez-Gutierrez Rotavirus 2019.pdfMartinez-Gutierrez Rotavirus 2019.pdfTrabajo de gradoapplication/pdf607156https://repository.ucc.edu.co/bitstreams/3e5cbcfb-e968-4847-8652-33cf9eb810d0/downloadeecc6b2b98bb8481374d8545d36b6812MD51Licencia_de_uso_Rotavirus.pdfLicencia_de_uso_Rotavirus.pdfFormato 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