Micropatterned silica thin films with nanohydroxyapatite micro-aggregates for guided tissue regeneration

Surface modification of biomaterials has been shown to improve the biological response to dental implants. The ability to create a controlled micro-texture on the implant via additive surface modification techniques with bioactive nanohydroxyapatite (nanoHA) may positively influence guided tissue regeneration. Objective: The main goal of this study was to produce micro-fabricated SiO 2 surfaces modified with nanohydroxyapatite particles and to characterize their influence on the biological response of Human Dental-Pulp Mesenchymal Stem Cells (hDP-MSCs) and Streptococcus mutans. Materials and methods: A combined methodology of sol-gel and soft-lithography was used to produce micropatterned SiO 2 thin films with different percentages of nanoHA micro-aggregates. The surfaces were characterized by SEM/EDS, FT-IR/ATR, AFM, XPS quantitative elemental percentage and contact angle measurements. Biological characterization was performed using hDP-MSCs cultures, while Streptococcus mutans was the selected microorganism to evaluate the bacterial adhesion on the thin films. Results: Micropatterned SiO 2 surfaces with 0%, 1% and 5% of nanoHA micro-aggregates were successfully produced using a combination of sol-gel and soft-lithography. These surfaces controlled the biological response, triggering alignment and oriented proliferation of hDP-MSCs and significant differences in the adhesion of S. mutans to the different surfaces. Significance: The micropatterned surfaces exhibited biocompatible behavior that induced an oriented adhesion and proliferation of hDP-MSCs while SiO 2 presented low bacterial adhesion. These results show that the combination of sol-gel with soft-lithography is a good approach to create micropatterned surfaces with bioactive nanoparticles for guided tissue regeneration. © 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

• Autores: Carvalho A.; Pelaez Vargas, Alejandro; Gallego-Perez D.; Grenho L.; Fernandes M.H.; De Aza A.H.; Ferraz M.P.; Hansford D.J.; Monteiro F.J.
• Tipo de recurso: Article of journal
• Fecha de publicación: 2023
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/49721
• Acceso en línea: https://doi.org/10.1016/j.dental.2012.09.002; https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867841771&doi=10.1016%2fj.dental.2012.09.002&partnerID=40&md5=0a77601fe851cc3b67ecc5d01365795f; https://hdl.handle.net/20.500.12494/49721
• Palabra clave: ADHESION; AFM; AGGREGATES; ANISOTROPY; ARTICLE; ATOMIC FORCE MICROSCOPY; BACTERIA; BACTERIAL ADHESION; BACTERIUM ADHERENCE; BIOACTIVE NANOPARTICLES; BIOFILM; BIOLOGICAL CHARACTERIZATION; BIOLOGICAL MATERIALS; BIOLOGICAL RESPONSE; BIOMATERIAL; BIOMATERIALS; CELL ADHESION; CELL COUNT; CELL CULTURE; CELL PROLIFERATION; CELL SURVIVAL; COMPUTER AIDED DESIGN; CONTACT ANGLE; CYTOLOGY; CHEMISTRY; DENTAL MATERIAL; DENTAL PROSTHESES; FLOWCHARTING; FTIR/ATR; GUIDED TISSUE REGENERATION; HUMAN; HYDROXYAPATITE; INFRARED SPECTROSCOPY; LITHOGRAPHY; MATERIALS TESTING; MESENCHYMAL STEM CELL; MESENCHYMAL STROMA CELL; METHODOLOGY; MICRO PATTERNING; MICROAGGREGATES; MICROPATTERNED; MICROPATTERNED SURFACE; MICRO-TEXTURE; NANO-HA; NANO-HYDROXYAPATITE; NANOMATERIAL; PHASE TRANSITION; PHYSIOLOGY; S; S. MUTANS; SCANNING ELECTRON MICROSCOPY; SEM/EDS; SILICA; SILICA THIN FILMS; SILICON DIOXIDE; SOFT-LITHOGRAPHY; SOL-GEL PROCESS; SOL-GELS; STREPTOCOCCUS MUTANS; SURFACE MODIFICATION TECHNIQUES; SURFACES; THIN FILMS
• Rights: openAccess
• License: http://purl.org/coar/access_right/c_abf2