Antiplasmodial effect of the venom of Crotalus durissus cumanensis, crotoxin complex and Crotoxin B

The antiplasmodial activity of phospholipases A2 (PLA2) isolated from different animals has been studied. We explored the in vitro anti Plasmodium falciparum effect of a fraction containing crotoxin, Crotoxin B and whole venom of the rattlesnake Crotalus durissus cumanensis. Fraction II (crotoxin co...

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Autores:
Quintana Castillo, Juan carlos
Chacón A.M.
Vargas L.
Segura C.
Gutiérrez J.M.
Alarcón J.C.
Tipo de recurso:
Article of journal
Fecha de publicación:
2023
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/49722
Acceso en línea:
https://doi.org/10.1016/j.actatropica.2012.07.003
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865739450&doi=10.1016%2fj.actatropica.2012.07.003&partnerID=40&md5=d6c65ed5de280e8ea246ae09baa87edf
https://hdl.handle.net/20.500.12494/49722
Palabra clave:
ACUTE TOXICITY
AMINO ACID SEQUENCE
ANIMAL EXPERIMENT
ANIMALIA
ANIMALS
ANTIMALARIAL ACTIVITY
ANTIMALARIAL AGENT
ANTIMALARIALS
ARTICLE
CELL SURVIVAL
CELLS, CULTURED
CONCENTRATION RESPONSE
CONTROLLED STUDY
CROTALUS
CROTALUS DURISSUS CUMANENSIS
CROTOXIN
CROTOXIN B
CROTOXIN COMPLEX
DRUG CYTOTOXICITY
DRUG EFFECT
DRUG PURIFICATION
DRUG SCREENING
GEL PERMEATION CHROMATOGRAPHY
HUMAN
HUMAN CELL
HUMANS
IN VITRO STUDY
LD 50
LETHALITY
LEUKOCYTES, MONONUCLEAR
MALARIA
MICE
MOLECULAR SEQUENCE DATA
MOUSE
MUS
NEUROTOXICITY
NONHUMAN
PARASITE
PERIPHERAL BLOOD MONONUCLEAR CELL
PLASMODIUM FALCIPARUM
REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
SNAKE
SNAKE VENOM
SURVIVAL ANALYSIS
TOXICITY
UNCLASSIFIED DRUG
VENOM
VENOMS
Rights
openAccess
License
http://purl.org/coar/access_right/c_abf2
id COOPER2_7e6662bd40d0261754feb4624fdfd694
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/49722
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
spelling Quintana Castillo, Juan carlosChacón A.M.Vargas L.Segura C.Gutiérrez J.M.Alarcón J.C.2023-05-24T16:21:40Z2023-05-24T16:21:40Z01/11/2012https://doi.org/10.1016/j.actatropica.2012.07.003https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865739450&doi=10.1016%2fj.actatropica.2012.07.003&partnerID=40&md5=d6c65ed5de280e8ea246ae09baa87edf0001706Xhttps://hdl.handle.net/20.500.12494/49722Quintana Castillo Juan carlos,Chacón A.M.,Vargas L.,Segura C.,Gutiérrez J.M.,Alarcón J.C..Antiplasmodial effect of the venom of Crotalus durissus cumanensis, crotoxin complex and Crotoxin B.ACTA TROP. 2012. 124. (2):p. 126-132The antiplasmodial activity of phospholipases A2 (PLA2) isolated from different animals has been studied. We explored the in vitro anti Plasmodium falciparum effect of a fraction containing crotoxin, Crotoxin B and whole venom of the rattlesnake Crotalus durissus cumanensis. Fraction II (crotoxin complex) was obtained by size exclusion chromatography, whereas Crotoxin B was purified by RP-HPLC. The whole venom is active against the parasite at concentrations of 0.17±0.03µg/ml, fraction II at 0.76±0.17µg/ml and Crotoxin B at 0.6±0.04µg/ml. Differences were observed in the cytotoxic activity against peripheral mononuclear cells, with Crotoxin B exhibiting the highest cytotoxicity. The concentration of Crotoxin B required to exert cytotoxic activity was higher than that required to exert antiplasmodial activity. Lethality in mice confirmed the higher toxicity and neurotoxicity of whole venom and fraction II, whereas Crotoxin B was not lethal at the doses tested. These results suggest the potential of Crotoxin B as a lead compound for antimalarial activity. © 2012 Elsevier B.V..0000-0002-7923-9158juan.quintanac@campusucc.edu.co126-132ElsevierACUTE TOXICITYAMINO ACID SEQUENCEANIMAL EXPERIMENTANIMALIAANIMALSANTIMALARIAL ACTIVITYANTIMALARIAL AGENTANTIMALARIALSARTICLECELL SURVIVALCELLS, CULTUREDCONCENTRATION RESPONSECONTROLLED STUDYCROTALUSCROTALUS DURISSUS CUMANENSISCROTOXINCROTOXIN BCROTOXIN COMPLEXDRUG CYTOTOXICITYDRUG EFFECTDRUG PURIFICATIONDRUG SCREENINGGEL PERMEATION CHROMATOGRAPHYHUMANHUMAN CELLHUMANSIN VITRO STUDYLD 50LETHALITYLEUKOCYTES, MONONUCLEARMALARIAMICEMOLECULAR SEQUENCE DATAMOUSEMUSNEUROTOXICITYNONHUMANPARASITEPERIPHERAL BLOOD MONONUCLEAR CELLPLASMODIUM FALCIPARUMREVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHYSNAKESNAKE VENOMSURVIVAL ANALYSISTOXICITYUNCLASSIFIED DRUGVENOMVENOMSAntiplasmodial effect of the venom of Crotalus durissus cumanensis, crotoxin complex and Crotoxin BArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionACTA TROPinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Publication20.500.12494/49722oai:repository.ucc.edu.co:20.500.12494/497222024-08-20 16:19:29.892metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv Antiplasmodial effect of the venom of Crotalus durissus cumanensis, crotoxin complex and Crotoxin B
title Antiplasmodial effect of the venom of Crotalus durissus cumanensis, crotoxin complex and Crotoxin B
spellingShingle Antiplasmodial effect of the venom of Crotalus durissus cumanensis, crotoxin complex and Crotoxin B
ACUTE TOXICITY
AMINO ACID SEQUENCE
ANIMAL EXPERIMENT
ANIMALIA
ANIMALS
ANTIMALARIAL ACTIVITY
ANTIMALARIAL AGENT
ANTIMALARIALS
ARTICLE
CELL SURVIVAL
CELLS, CULTURED
CONCENTRATION RESPONSE
CONTROLLED STUDY
CROTALUS
CROTALUS DURISSUS CUMANENSIS
CROTOXIN
CROTOXIN B
CROTOXIN COMPLEX
DRUG CYTOTOXICITY
DRUG EFFECT
DRUG PURIFICATION
DRUG SCREENING
GEL PERMEATION CHROMATOGRAPHY
HUMAN
HUMAN CELL
HUMANS
IN VITRO STUDY
LD 50
LETHALITY
LEUKOCYTES, MONONUCLEAR
MALARIA
MICE
MOLECULAR SEQUENCE DATA
MOUSE
MUS
NEUROTOXICITY
NONHUMAN
PARASITE
PERIPHERAL BLOOD MONONUCLEAR CELL
PLASMODIUM FALCIPARUM
REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
SNAKE
SNAKE VENOM
SURVIVAL ANALYSIS
TOXICITY
UNCLASSIFIED DRUG
VENOM
VENOMS
title_short Antiplasmodial effect of the venom of Crotalus durissus cumanensis, crotoxin complex and Crotoxin B
title_full Antiplasmodial effect of the venom of Crotalus durissus cumanensis, crotoxin complex and Crotoxin B
title_fullStr Antiplasmodial effect of the venom of Crotalus durissus cumanensis, crotoxin complex and Crotoxin B
title_full_unstemmed Antiplasmodial effect of the venom of Crotalus durissus cumanensis, crotoxin complex and Crotoxin B
title_sort Antiplasmodial effect of the venom of Crotalus durissus cumanensis, crotoxin complex and Crotoxin B
dc.creator.fl_str_mv Quintana Castillo, Juan carlos
Chacón A.M.
Vargas L.
Segura C.
Gutiérrez J.M.
Alarcón J.C.
dc.contributor.author.none.fl_str_mv Quintana Castillo, Juan carlos
Chacón A.M.
Vargas L.
Segura C.
Gutiérrez J.M.
Alarcón J.C.
dc.subject.spa.fl_str_mv ACUTE TOXICITY
AMINO ACID SEQUENCE
ANIMAL EXPERIMENT
ANIMALIA
ANIMALS
ANTIMALARIAL ACTIVITY
ANTIMALARIAL AGENT
ANTIMALARIALS
ARTICLE
CELL SURVIVAL
CELLS, CULTURED
CONCENTRATION RESPONSE
CONTROLLED STUDY
CROTALUS
CROTALUS DURISSUS CUMANENSIS
CROTOXIN
CROTOXIN B
CROTOXIN COMPLEX
DRUG CYTOTOXICITY
DRUG EFFECT
DRUG PURIFICATION
DRUG SCREENING
GEL PERMEATION CHROMATOGRAPHY
HUMAN
HUMAN CELL
HUMANS
IN VITRO STUDY
LD 50
LETHALITY
LEUKOCYTES, MONONUCLEAR
MALARIA
MICE
MOLECULAR SEQUENCE DATA
MOUSE
MUS
NEUROTOXICITY
NONHUMAN
PARASITE
PERIPHERAL BLOOD MONONUCLEAR CELL
PLASMODIUM FALCIPARUM
REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
SNAKE
SNAKE VENOM
SURVIVAL ANALYSIS
TOXICITY
UNCLASSIFIED DRUG
VENOM
VENOMS
topic ACUTE TOXICITY
AMINO ACID SEQUENCE
ANIMAL EXPERIMENT
ANIMALIA
ANIMALS
ANTIMALARIAL ACTIVITY
ANTIMALARIAL AGENT
ANTIMALARIALS
ARTICLE
CELL SURVIVAL
CELLS, CULTURED
CONCENTRATION RESPONSE
CONTROLLED STUDY
CROTALUS
CROTALUS DURISSUS CUMANENSIS
CROTOXIN
CROTOXIN B
CROTOXIN COMPLEX
DRUG CYTOTOXICITY
DRUG EFFECT
DRUG PURIFICATION
DRUG SCREENING
GEL PERMEATION CHROMATOGRAPHY
HUMAN
HUMAN CELL
HUMANS
IN VITRO STUDY
LD 50
LETHALITY
LEUKOCYTES, MONONUCLEAR
MALARIA
MICE
MOLECULAR SEQUENCE DATA
MOUSE
MUS
NEUROTOXICITY
NONHUMAN
PARASITE
PERIPHERAL BLOOD MONONUCLEAR CELL
PLASMODIUM FALCIPARUM
REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
SNAKE
SNAKE VENOM
SURVIVAL ANALYSIS
TOXICITY
UNCLASSIFIED DRUG
VENOM
VENOMS
description The antiplasmodial activity of phospholipases A2 (PLA2) isolated from different animals has been studied. We explored the in vitro anti Plasmodium falciparum effect of a fraction containing crotoxin, Crotoxin B and whole venom of the rattlesnake Crotalus durissus cumanensis. Fraction II (crotoxin complex) was obtained by size exclusion chromatography, whereas Crotoxin B was purified by RP-HPLC. The whole venom is active against the parasite at concentrations of 0.17±0.03µg/ml, fraction II at 0.76±0.17µg/ml and Crotoxin B at 0.6±0.04µg/ml. Differences were observed in the cytotoxic activity against peripheral mononuclear cells, with Crotoxin B exhibiting the highest cytotoxicity. The concentration of Crotoxin B required to exert cytotoxic activity was higher than that required to exert antiplasmodial activity. Lethality in mice confirmed the higher toxicity and neurotoxicity of whole venom and fraction II, whereas Crotoxin B was not lethal at the doses tested. These results suggest the potential of Crotoxin B as a lead compound for antimalarial activity. © 2012 Elsevier B.V..
publishDate 2023
dc.date.issued.none.fl_str_mv 01/11/2012
dc.date.accessioned.none.fl_str_mv 2023-05-24T16:21:40Z
dc.date.available.none.fl_str_mv 2023-05-24T16:21:40Z
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.actatropica.2012.07.003
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865739450&doi=10.1016%2fj.actatropica.2012.07.003&partnerID=40&md5=d6c65ed5de280e8ea246ae09baa87edf
dc.identifier.issn.spa.fl_str_mv 0001706X
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/49722
dc.identifier.bibliographicCitation.spa.fl_str_mv Quintana Castillo Juan carlos,Chacón A.M.,Vargas L.,Segura C.,Gutiérrez J.M.,Alarcón J.C..Antiplasmodial effect of the venom of Crotalus durissus cumanensis, crotoxin complex and Crotoxin B.ACTA TROP. 2012. 124. (2):p. 126-132
url https://doi.org/10.1016/j.actatropica.2012.07.003
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865739450&doi=10.1016%2fj.actatropica.2012.07.003&partnerID=40&md5=d6c65ed5de280e8ea246ae09baa87edf
https://hdl.handle.net/20.500.12494/49722
identifier_str_mv 0001706X
Quintana Castillo Juan carlos,Chacón A.M.,Vargas L.,Segura C.,Gutiérrez J.M.,Alarcón J.C..Antiplasmodial effect of the venom of Crotalus durissus cumanensis, crotoxin complex and Crotoxin B.ACTA TROP. 2012. 124. (2):p. 126-132
dc.relation.ispartofjournal.spa.fl_str_mv ACTA TROP
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_abf2
dc.format.extent.spa.fl_str_mv 126-132
dc.publisher.spa.fl_str_mv Elsevier
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
_version_ 1814246963286114304

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