Characterizing the biological and biochemical profile of six different scorpion venoms from the Buthidae and Scorpionidae family
Artículo cientifico
- Autores:
-
Estrada Gómez, Sebastián
Gómez Rave, Lyz
Vargas Muñoz, Leidy Johana
Van der Meijden, Arie
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2017
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/3365
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/3365
- Palabra clave:
- Grosphus
Post-translational modifications
Babycurus
Hottentotta
Tityus
Metalloproteinases
Phospholipases
Antitumor MCF-7
- Rights
- openAccess
- License
- Licencia CC
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| dc.title.spa.fl_str_mv |
Characterizing the biological and biochemical profile of six different scorpion venoms from the Buthidae and Scorpionidae family |
| title |
Characterizing the biological and biochemical profile of six different scorpion venoms from the Buthidae and Scorpionidae family |
| spellingShingle |
Characterizing the biological and biochemical profile of six different scorpion venoms from the Buthidae and Scorpionidae family Grosphus Post-translational modifications Babycurus Hottentotta Tityus Metalloproteinases Phospholipases Antitumor MCF-7 |
| title_short |
Characterizing the biological and biochemical profile of six different scorpion venoms from the Buthidae and Scorpionidae family |
| title_full |
Characterizing the biological and biochemical profile of six different scorpion venoms from the Buthidae and Scorpionidae family |
| title_fullStr |
Characterizing the biological and biochemical profile of six different scorpion venoms from the Buthidae and Scorpionidae family |
| title_full_unstemmed |
Characterizing the biological and biochemical profile of six different scorpion venoms from the Buthidae and Scorpionidae family |
| title_sort |
Characterizing the biological and biochemical profile of six different scorpion venoms from the Buthidae and Scorpionidae family |
| dc.creator.fl_str_mv |
Estrada Gómez, Sebastián Gómez Rave, Lyz Vargas Muñoz, Leidy Johana Van der Meijden, Arie |
| dc.contributor.author.none.fl_str_mv |
Estrada Gómez, Sebastián Gómez Rave, Lyz Vargas Muñoz, Leidy Johana Van der Meijden, Arie |
| dc.subject.spa.fl_str_mv |
Grosphus Post-translational modifications Babycurus Hottentotta Tityus Metalloproteinases Phospholipases Antitumor MCF-7 |
| topic |
Grosphus Post-translational modifications Babycurus Hottentotta Tityus Metalloproteinases Phospholipases Antitumor MCF-7 |
| description |
Artículo cientifico |
| publishDate |
2017 |
| dc.date.issued.none.fl_str_mv |
2017-02-13 |
| dc.date.accessioned.none.fl_str_mv |
2018-04-10T19:19:05Z |
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2018-04-10T19:19:05Z |
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Artículo |
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https://hdl.handle.net/20.500.12494/3365 |
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Estrada Gómez, S., Gómez Rave, L., Vargas Muñoz, L. J., y Van der Meijden. A. (2017). Characterizing the biological and biochemical profile of six different scorpion venoms from the Buthidae and Scorpionidae family. Toxicon 130, 104-115. http://dx.doi.org/10.1016/j.toxicon.2017.02.007 |
| url |
https://hdl.handle.net/20.500.12494/3365 |
| identifier_str_mv |
Estrada Gómez, S., Gómez Rave, L., Vargas Muñoz, L. J., y Van der Meijden. A. (2017). Characterizing the biological and biochemical profile of six different scorpion venoms from the Buthidae and Scorpionidae family. Toxicon 130, 104-115. http://dx.doi.org/10.1016/j.toxicon.2017.02.007 |
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https://www.sciencedirect.com/science/article/pii/S0041010117300545?via%3Dihub |
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Licencia CC |
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info:eu-repo/semantics/openAccess |
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http://purl.org/coar/access_right/c_abf2 |
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Licencia CC http://purl.org/coar/access_right/c_abf2 |
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openAccess |
| dc.publisher.spa.fl_str_mv |
Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Medellín y Envigado |
| dc.publisher.program.spa.fl_str_mv |
Medicina |
| dc.publisher.place.spa.fl_str_mv |
Medellín |
| institution |
Universidad Cooperativa de Colombia |
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Estrada Gómez, SebastiánGómez Rave, LyzVargas Muñoz, Leidy JohanaVan der Meijden, Arie2018-04-10T19:19:05Z2018-04-10T19:19:05Z2017-02-13https://hdl.handle.net/20.500.12494/3365Estrada Gómez, S., Gómez Rave, L., Vargas Muñoz, L. J., y Van der Meijden. A. (2017). Characterizing the biological and biochemical profile of six different scorpion venoms from the Buthidae and Scorpionidae family. Toxicon 130, 104-115. http://dx.doi.org/10.1016/j.toxicon.2017.02.007Artículo cientificoThe objective of this study was to characterize six different scorpion venoms using biological and biochemical methods, including a preliminary MS/MS and a post-translational modifications analysis. Despite the diversity of scorpion species of medical importance in Africa and Colombia, the venoms of these arachnids have been poorly studied in these two regions. We report the biochemical, electrophoretic, chromatographic profile, internal peptide sequences with a post-translational modification report, and a preliminary antitumor activity of five different scorpions of the Buthidae family, Androctonus amoreuxi, Babycurus jacksoni, Grosphus grandidieri, Hottentotta gentili and Tityus fuhrmanni, and one of the Scorpionidae family Pandinus imperator. No L-amino oxidase activity was detected in the evaluated venoms. Proteolytic activity using azocasein was detected only in G. grandidieri and P. imperator, indicating the possible presence of metalloproteinases in these two venoms. Proteolytic activity using NOBA was detected in all venoms indicating the possible presence of serine-proteinases. Phospholipase A2 activity was detected in the venoms of P. imperator, G. grandidieri, H. gentili and A. amoreuxi, with P. imperator venom being the most active. All venoms analyzed contained defensin-like proteins, alpha toxins, metalloproteinases, neuropeptides, DBP affecting ion channels, DBP with antimicrobial activity, among others. Venoms from P. imperator, G. grandidieri and T. fuhrmanni showed a dose-dependent cytotoxic activity over MCF-7 cells. Only two isolated RP-HPLC fractions from P. imperator and T. fuhrmanni showed cytotoxic activity over MCF-7. No cytotoxic activity was found in the venoms from A. amoreuxi, B. jacksoni, and H. gentili.sebastian.estrada@udea.edu.colyzjegora@gmail.comleidy.vargasmu@campusucc.edu.comail@arievandermeijden.nlUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Medellín y EnvigadoMedicinaMedellínhttps://www.sciencedirect.com/science/article/pii/S0041010117300545?via%3DihubGrosphusPost-translational modificationsBabycurusHottentottaTityusMetalloproteinasesPhospholipasesAntitumor MCF-7Characterizing the biological and biochemical profile of six different scorpion venoms from the Buthidae and Scorpionidae familyArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionLicencia CCinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationTHUMBNAILCharacterizing the biological and biochemical profile of six different.pdf.jpgCharacterizing the biological and biochemical profile of six different.pdf.jpgIM Thumbnailimage/jpeg6121https://repository.ucc.edu.co/bitstreams/176fb376-3119-4cbb-a880-32055ba219d7/downloade10c4be114709b276c13536b68f114c5MD55Formato Licencia de uso.pdf.jpgFormato Licencia de uso.pdf.jpgIM Thumbnailimage/jpeg5313https://repository.ucc.edu.co/bitstreams/863df513-2786-4059-bc6a-0f15c20e5078/downloadcfb79eba048755a48755b0af7d620784MD57ORIGINALCharacterizing the biological and biochemical profile of six different.pdfCharacterizing the biological and biochemical profile of six different.pdfapplication/pdf2253579https://repository.ucc.edu.co/bitstreams/a027cbf8-ce01-40b8-add0-ee78e3144340/download3fbe31fcf74ec6499f40637d7075f6e1MD51Formato Licencia de uso.pdfFormato Licencia de uso.pdfapplication/pdf215914https://repository.ucc.edu.co/bitstreams/c26637fc-cf9a-4f89-94a8-0ad4b5002d19/downloadb9be3f3834de28f7c374f809ae0bd756MD53LICENSElicense.txtlicense.txttext/plain; charset=utf-84514https://repository.ucc.edu.co/bitstreams/571e3b62-f1da-402a-94ab-1400c73f70ff/downloadf3f5e184cacb7496a3e3345ca148d0f7MD52TEXTCharacterizing the biological and biochemical profile of six different.pdf.txtCharacterizing the biological and biochemical profile of six different.pdf.txtExtracted texttext/plain67100https://repository.ucc.edu.co/bitstreams/8b636011-6a96-4b55-94dd-c6f09fddc6d7/download6a49824cd4f83d8730a3b56b9b0c91beMD54Formato Licencia de uso.pdf.txtFormato Licencia de uso.pdf.txtExtracted texttext/plain4392https://repository.ucc.edu.co/bitstreams/24fa244c-27d4-4448-b41e-bae8439ac218/download2c43f0d1167c388d6994b2d4b0bd22efMD5620.500.12494/3365oai:repository.ucc.edu.co:20.500.12494/33652024-08-10 22:46:17.697open.accesshttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com |