First detection and full genomic analysis of canine circovirus in CPV-2 infected dogs in Colombia, South America
Canine Circovirus (CanineCV) is an emerging virus which since its first report in USA in 2012, it has been described worldwide. It was the second mammalian circovirus species identified in dogs and its role in canine enteritis is still being uncertain as much as its association in disease with the C...
- Autores:
-
Giraldo Ramírez, Sebastián
Rendón Marín, Santiago
Vargas Bermudez, Diana Susana
Jaime, Jairo
Ruiz Sáenz, Julián
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2020
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/32932
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/32932
- Palabra clave:
- Genomic analysis
Canine circovirus
Dogs
Emerging virus
- Rights
- openAccess
- License
- Atribución – No comercial – Compartir igual
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First detection and full genomic analysis of canine circovirus in CPV-2 infected dogs in Colombia, South America |
| title |
First detection and full genomic analysis of canine circovirus in CPV-2 infected dogs in Colombia, South America |
| spellingShingle |
First detection and full genomic analysis of canine circovirus in CPV-2 infected dogs in Colombia, South America Genomic analysis Canine circovirus Dogs Emerging virus |
| title_short |
First detection and full genomic analysis of canine circovirus in CPV-2 infected dogs in Colombia, South America |
| title_full |
First detection and full genomic analysis of canine circovirus in CPV-2 infected dogs in Colombia, South America |
| title_fullStr |
First detection and full genomic analysis of canine circovirus in CPV-2 infected dogs in Colombia, South America |
| title_full_unstemmed |
First detection and full genomic analysis of canine circovirus in CPV-2 infected dogs in Colombia, South America |
| title_sort |
First detection and full genomic analysis of canine circovirus in CPV-2 infected dogs in Colombia, South America |
| dc.creator.fl_str_mv |
Giraldo Ramírez, Sebastián Rendón Marín, Santiago Vargas Bermudez, Diana Susana Jaime, Jairo Ruiz Sáenz, Julián |
| dc.contributor.author.none.fl_str_mv |
Giraldo Ramírez, Sebastián Rendón Marín, Santiago Vargas Bermudez, Diana Susana Jaime, Jairo Ruiz Sáenz, Julián |
| dc.subject.spa.fl_str_mv |
Genomic analysis Canine circovirus Dogs Emerging virus |
| topic |
Genomic analysis Canine circovirus Dogs Emerging virus |
| description |
Canine Circovirus (CanineCV) is an emerging virus which since its first report in USA in 2012, it has been described worldwide. It was the second mammalian circovirus species identified in dogs and its role in canine enteritis is still being uncertain as much as its association in disease with the Canine Parvovirus-2 (CPV-2). Here, we aim to confirm for the first time the presence of CanineCV in Colombia and to develop phylogenetic evolutive analyses of CanineCV in CPV-2 positive animals. DNA from samples were extracted and PCR, full genome sequencing and phylogenetic analysis was performed to detect and characterize CanineCV. From a total of 30 CPV-2 positive samples, 16.6% (n = 5) were positives for CanineCV. Sequencing analysis of Colombian CanineCV wild-type strains displayed high identity to each other (99.5–99.7% nt; 99.7% aa). The full genome phylogenetic analysis confirmed that worldwide reported CanineCV strains were separated into four distinct genotypes in addition to a European origin of the South American CanineCV strains. This study demonstrated the importance of continue surveillance of emerging viruses in canine populations and confirm for the first time the circulation and origin of CanineCV in Colombia. |
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2020 |
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2020-10-16 |
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2021-01-28T16:59:44Z |
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2021-01-28T16:59:44Z |
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Artículos Científicos |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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2045-2322 |
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doi.org/10.1038/s41598-020-74630-8 |
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https://hdl.handle.net/20.500.12494/32932 |
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Giraldo-Ramirez, S., Rendon-Marin, S., Vargas-Bermudez, D. S., Jaime, J., & Ruiz-Saenz, J. (2020). First detection and full genomic analysis of Canine Circovirus in CPV-2 infected dogs in Colombia, South America. Scientific Reports, 10(1), 1-9. |
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2045-2322 doi.org/10.1038/s41598-020-74630-8 Giraldo-Ramirez, S., Rendon-Marin, S., Vargas-Bermudez, D. S., Jaime, J., & Ruiz-Saenz, J. (2020). First detection and full genomic analysis of Canine Circovirus in CPV-2 infected dogs in Colombia, South America. Scientific Reports, 10(1), 1-9. |
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https://hdl.handle.net/20.500.12494/32932 |
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https://www.nature.com/articles/s41598-020-74630-8 |
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Scientific reports |
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Kapoor, A., Dubovi, E. J., Henriquez-Rivera, J. A. & Lipkin, W. I. Complete genome sequence of the first canine circovirus. J Virol 86, 7018. https://doi.org/10.1128/JVI.00791-12 (2012). Li, L. et al. Circovirus in tissues of dogs with vasculitis and hemorrhage. Emerg. Infect. Dis. 19, 534–541. https://doi.org/10.3201/eid1904.121390 (2013). Biagini, P. et al. Family circoviridae. In Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses, 343–349 (2012). Lecis, R. et al. Genomic characterization of a novel bat-associated Circovirus detected in European Miniopterus schreibersii bats. Virus Genes 56, 325–328. https://doi.org/10.1007/s11262-020-01747-3 (2020). Zhu, A. et al. Molecular characterization of a novel bat-associated circovirus with a poly-T tract in the 3’ intergenic region. Virus Res. 250, 95–103. https://doi.org/10.1016/j.virusres.2018.04.012 (2018). Palinski, R. et al. A novel porcine circovirus distantly related to known circoviruses is associated with porcine dermatitis and nephropathy syndrome and reproductive failure. J. Virol. 91, e01879-e1816 (2017). Zhai, S. L. et al. Reservoirs of porcine circoviruses: a mini review. Front. Vet. Sci. 6, 319. https://doi.org/10.3389/fvets.2019.00319 (2019). Herbst, W. & Willems, H. Detection of virus particles resembling circovirus and porcine circovirus 2a (PCV2a) sequences in feces of dogs. Res. Vet. Sci. 115, 51–53. https://doi.org/10.1016/j.rvsc.2017.01.014 (2017). Zhang, J. et al. First molecular detection of porcine circovirus type 3 in dogs in China. Virus Genes 54, 140–144. https://doi.org/10.1007/s11262-017-1509-0 (2018). Sun, W. et al. An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017, China. Virus Res. 270, 197663. https://doi.org/10.1016/j.virusres.2019.197663 (2019). Decaro, N. et al. Genomic characterization of a circovirus associated with fatal hemorrhagic enteritis in dog, Italy. PLoS ONE 9, e105909. https://doi.org/10.1371/journal.pone.0105909 (2014). Duque-Garcia, Y., Echeverri-Zuluaga, M., Trejos-Suarez, J. & Ruiz-Saenz, J. Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: a possible new CPV-2a is emerging?. Vet. Microbiol. 201, 56–61. https://doi.org/10.1016/j.vetmic.2016.12.039 (2017). Weaver, S. et al. Datamonkey 2.0: a modern web application for characterizing selective and other evolutionary processes. Mol. Biol. Evol. 35, 773–777. https://doi.org/10.1093/molbev/msx335 (2018). Giraldo-Ramirez, S., Rendon-Marin, S. & Ruiz-Saenz, J. Phylogenetic, evolutionary and structural analysis of Canine Parvovirus (CPV-2) antigenic variants circulating in Colombia. Viruses https://doi.org/10.3390/v12050500 (2020). Zhou, P., Zeng, W., Zhang, X. & Li, S. The genetic evolution of canine parvovirus—a new perspective. PLoS ONE 12, e0175035. https://doi.org/10.1371/journal.pone.0175035 (2017). |
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Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, Bucaramanga Springer/Nature |
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Giraldo Ramírez, SebastiánRendón Marín, SantiagoVargas Bermudez, Diana SusanaJaime, JairoRuiz Sáenz, Julián10 2021-01-28T16:59:44Z2021-01-28T16:59:44Z2020-10-162045-2322doi.org/10.1038/s41598-020-74630-8https://hdl.handle.net/20.500.12494/32932Giraldo-Ramirez, S., Rendon-Marin, S., Vargas-Bermudez, D. S., Jaime, J., & Ruiz-Saenz, J. (2020). First detection and full genomic analysis of Canine Circovirus in CPV-2 infected dogs in Colombia, South America. Scientific Reports, 10(1), 1-9.Canine Circovirus (CanineCV) is an emerging virus which since its first report in USA in 2012, it has been described worldwide. It was the second mammalian circovirus species identified in dogs and its role in canine enteritis is still being uncertain as much as its association in disease with the Canine Parvovirus-2 (CPV-2). Here, we aim to confirm for the first time the presence of CanineCV in Colombia and to develop phylogenetic evolutive analyses of CanineCV in CPV-2 positive animals. DNA from samples were extracted and PCR, full genome sequencing and phylogenetic analysis was performed to detect and characterize CanineCV. From a total of 30 CPV-2 positive samples, 16.6% (n = 5) were positives for CanineCV. Sequencing analysis of Colombian CanineCV wild-type strains displayed high identity to each other (99.5–99.7% nt; 99.7% aa). The full genome phylogenetic analysis confirmed that worldwide reported CanineCV strains were separated into four distinct genotypes in addition to a European origin of the South American CanineCV strains. This study demonstrated the importance of continue surveillance of emerging viruses in canine populations and confirm for the first time the circulation and origin of CanineCV in Colombia.http://scienti.colciencias.gov.co:8081/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000153095https://orcid.org/0000-0002-1447-1458https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000000695julian.ruizs@campusucc.edu.cohttps://scholar.google.com/citations?user=o3Y7mZwAAAAJ&hl=es17579Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, BucaramangaSpringer/NatureMedicina veterinaria y zootecniaBucaramangahttps://www.nature.com/articles/s41598-020-74630-8Scientific reportsKapoor, A., Dubovi, E. J., Henriquez-Rivera, J. A. & Lipkin, W. I. Complete genome sequence of the first canine circovirus. J Virol 86, 7018. https://doi.org/10.1128/JVI.00791-12 (2012).Li, L. et al. Circovirus in tissues of dogs with vasculitis and hemorrhage. Emerg. Infect. Dis. 19, 534–541. https://doi.org/10.3201/eid1904.121390 (2013).Biagini, P. et al. Family circoviridae. In Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses, 343–349 (2012).Lecis, R. et al. Genomic characterization of a novel bat-associated Circovirus detected in European Miniopterus schreibersii bats. Virus Genes 56, 325–328. https://doi.org/10.1007/s11262-020-01747-3 (2020).Zhu, A. et al. Molecular characterization of a novel bat-associated circovirus with a poly-T tract in the 3’ intergenic region. Virus Res. 250, 95–103. https://doi.org/10.1016/j.virusres.2018.04.012 (2018).Palinski, R. et al. A novel porcine circovirus distantly related to known circoviruses is associated with porcine dermatitis and nephropathy syndrome and reproductive failure. J. Virol. 91, e01879-e1816 (2017).Zhai, S. L. et al. Reservoirs of porcine circoviruses: a mini review. Front. Vet. Sci. 6, 319. https://doi.org/10.3389/fvets.2019.00319 (2019).Herbst, W. & Willems, H. Detection of virus particles resembling circovirus and porcine circovirus 2a (PCV2a) sequences in feces of dogs. Res. Vet. Sci. 115, 51–53. https://doi.org/10.1016/j.rvsc.2017.01.014 (2017).Zhang, J. et al. First molecular detection of porcine circovirus type 3 in dogs in China. Virus Genes 54, 140–144. https://doi.org/10.1007/s11262-017-1509-0 (2018).Sun, W. et al. An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017, China. Virus Res. 270, 197663. https://doi.org/10.1016/j.virusres.2019.197663 (2019).Decaro, N. et al. Genomic characterization of a circovirus associated with fatal hemorrhagic enteritis in dog, Italy. PLoS ONE 9, e105909. https://doi.org/10.1371/journal.pone.0105909 (2014).Duque-Garcia, Y., Echeverri-Zuluaga, M., Trejos-Suarez, J. & Ruiz-Saenz, J. Prevalence and molecular epidemiology of Canine parvovirus 2 in diarrheic dogs in Colombia, South America: a possible new CPV-2a is emerging?. Vet. Microbiol. 201, 56–61. https://doi.org/10.1016/j.vetmic.2016.12.039 (2017).Weaver, S. et al. Datamonkey 2.0: a modern web application for characterizing selective and other evolutionary processes. Mol. Biol. Evol. 35, 773–777. https://doi.org/10.1093/molbev/msx335 (2018).Giraldo-Ramirez, S., Rendon-Marin, S. & Ruiz-Saenz, J. Phylogenetic, evolutionary and structural analysis of Canine Parvovirus (CPV-2) antigenic variants circulating in Colombia. Viruses https://doi.org/10.3390/v12050500 (2020).Zhou, P., Zeng, W., Zhang, X. & Li, S. The genetic evolution of canine parvovirus—a new perspective. PLoS ONE 12, e0175035. https://doi.org/10.1371/journal.pone.0175035 (2017).Genomic analysisCanine circovirusDogsEmerging virusFirst detection and full genomic analysis of canine circovirus in CPV-2 infected dogs in Colombia, South AmericaArtículos Científicoshttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribución – No comercial – Compartir igualinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationLICENSElicense.txtlicense.txttext/plain; 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