Serological and molecular diagnosis of the feline coronavirus in the americas
Since its discovery by Holzworth in 1962, the study of the Feline Coronavirus (FCoV) has have a great interest because it can affect wildlife and domestic felines. Currently 2 serotypes are known, type I is unique for felines and type II arose from a double homologous recombination with a canine cor...
- Autores:
-
Valencia, Alida Carolina
Delgado Villamizar, Karen
Ruiz Sáenz, Julián
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2021
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/43883
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/43883
- Palabra clave:
- Diagnostic
Feline Coronavirus
FIPV
FCoV
- Rights
- openAccess
- License
- Atribución – No comercial – Compartir igual
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Serological and molecular diagnosis of the feline coronavirus in the americas |
| title |
Serological and molecular diagnosis of the feline coronavirus in the americas |
| spellingShingle |
Serological and molecular diagnosis of the feline coronavirus in the americas Diagnostic Feline Coronavirus FIPV FCoV |
| title_short |
Serological and molecular diagnosis of the feline coronavirus in the americas |
| title_full |
Serological and molecular diagnosis of the feline coronavirus in the americas |
| title_fullStr |
Serological and molecular diagnosis of the feline coronavirus in the americas |
| title_full_unstemmed |
Serological and molecular diagnosis of the feline coronavirus in the americas |
| title_sort |
Serological and molecular diagnosis of the feline coronavirus in the americas |
| dc.creator.fl_str_mv |
Valencia, Alida Carolina Delgado Villamizar, Karen Ruiz Sáenz, Julián |
| dc.contributor.author.none.fl_str_mv |
Valencia, Alida Carolina Delgado Villamizar, Karen Ruiz Sáenz, Julián |
| dc.subject.spa.fl_str_mv |
Diagnostic Feline Coronavirus FIPV FCoV |
| topic |
Diagnostic Feline Coronavirus FIPV FCoV |
| description |
Since its discovery by Holzworth in 1962, the study of the Feline Coronavirus (FCoV) has have a great interest because it can affect wildlife and domestic felines. Currently 2 serotypes are known, type I is unique for felines and type II arose from a double homologous recombination with a canine coronavirus (CCoV); these also can be classified in 2 biotypes, viruses that generate mild enteric diseases (FECVs) and those that cause the feline infectious peritonitis (FIPVs). In the American continent exist different diagnostic methods that together allow the detection of the feline infectious peritonitis (FIP), but the identification of the FCoV only can be done by molecular methods. The countries that have studied this virus the most are those that have the greatest number of tools to carry out diagnostic test, such as United States, Canada and Brazil. In the present work are shown cases reports and the diagnostics methods used to identify the feline coronavirus and/or its biotypes in some countries of the American continent. |
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2021 |
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2021-04 |
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2022-02-09T12:20:54Z |
| dc.date.available.none.fl_str_mv |
2022-02-09T12:20:54Z |
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Artículos Científicos |
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doi.org/10.21897/rmvz.2041 |
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https://hdl.handle.net/20.500.12494/43883 |
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Valencia G, A., Delgado-Villamizar, K., & Ruiz-Saenz, J. (2021). Serological and molecular diagnosis of the feline coronavirus in the americas. Journal MVZ Cordoba, 26(2), e2041. https://doi.org/10.21897/rmvz.2041 |
| identifier_str_mv |
0122-0268 doi.org/10.21897/rmvz.2041 Valencia G, A., Delgado-Villamizar, K., & Ruiz-Saenz, J. (2021). Serological and molecular diagnosis of the feline coronavirus in the americas. Journal MVZ Cordoba, 26(2), e2041. https://doi.org/10.21897/rmvz.2041 |
| url |
https://hdl.handle.net/20.500.12494/43883 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
26 p. |
| dc.relation.references.spa.fl_str_mv |
Lemmermeyer T, Lamp B, Schneider R, Ziebuhr J, Tekes G, Thiel H-J. Characterization of monoclonal antibodies against feline coronavirus accessory protein 7b. Vet Microbiol. 2016; 184:11-19. https://doi.org/10.1016/j.vetmic.2015.12.009. Kipar A, Meli M. Feline infectious peritonitis: still an enigma? Vet Pathol. 2014; 51(2):505-526. https://doi.org/10.1177/0300985814522077. Jaimes JA, Whittaker GR. Feline coronavirus: insights into viral pathogenesis based on the spike protein structure and function. Virology. 2018; 517:108-121. Chang H-W, de Groot RJ, Egberink HF, Rottier PJ. Feline infectious peritonitis: insights into feline coronavirus pathobiogenesis and epidemiology based on genetic analysis of the viral 3c gene. J Gen Virol. 2010; 91(2):415-420. https://doi.org/10.1099/vir.0.016485-0. Shiba N, Maeda K, Kato H, Mochizuki M, Iwata H. Differentiation of feline coronavirus type I and II infections by virus neutralization test. Vet microbiol. 2007; 124(3-4):348-352. https://doi.org/10.1016/j.vetmic.2007.04.031. Tekes G, Thiel H-J. Feline coronaviruses: pathogenesis of feline infectious peritonitis. Advances in virus research. Elsevier. 2016; 96:193-218. https://doi.org/10.1016/bs.aivir.2016.08.002. Desmarets LM, Vermeulen BL, Theuns S, Conceição-Neto N, Zeller M, Roukaerts ID, et al. Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures. Sci Rep. 2016; 6(1):1-11. https://doi.org/10.1038/srep20022. Kim Y, Liu H, Kankanamalage ACG, Weerasekara S, Hua DH, Groutas WC, et al. Reversal of the progression of fatal coronavirus infection in cats by a broad-spectrum coronavirus protease inhibitor. PLoS Pathog. 2016; 12(3):1-18. https://doi.org/10.1371/journal.ppat.1005650. Licitra BN, Millet JK, Regan AD, Hamilton BS, Rinaldi VD, Duhamel GE, et al. Mutation in spike protein cleavage site and pathogenesis of feline coronavirus. Emerg Infect Dis. 2013; 19(7):1066-1073. http://dx.doi.org/10.3201/eid1907.121094. Sharif S, Arshad SS, Hair-Bejo M, Omar AR, Zeenathul NA, Alazawy A. Diagnostic methods for feline coronavirus: a review. Vet Med Int. 2010; 2010:1-8. https://doi.org/10.4061/2010/809480. Hartmann K. Feline infectious peritonitis. Vet Clin Small Anim. 2005; 35(1):39-79. https://doi.org/10.1016/j.cvsm.2004.10.011. Addie DD. Feline infectious peritonitis: answers to frequently asked questions concerning FIP and coronavirus. VNJ. 2019; 34(8):201-206. https://doi.org/10.1080/17415349.2019.1629366. Tasker S. Diagnosis of feline infectious peritonitis: Update on evidence supporting available tests. J Feline Med Surg. 2018; 20(3):228-243. https://doi.org/10.1177/1098612X18758592. Takano T, Hohdatsu T. Serological diagnosis of feline coronavirus infection by immunochromatographic test. Coronaviruses: Springer. 2015; 1282:33-39. https://doi.org/10.1007/978-1-4939-2438-7_3. Hora A, Tonietti P, Taniwaki S, Asano K, Maiorka P, Richtzenhain L, et al. Feline coronavirus 3C protein: a candidate for a virulence marker? BioMed Res Int. 2016; 9(8):1-9. http://dx.doi.org/10.1155/2016/8560691. Pedersen NC. An update on feline infectious peritonitis: diagnostics and therapeutics. Vet J. 2014; 201(2):133-141. https://doi.org/10.1016/j.tvjl.2014.04.016. Prieto M, Acuña A. Actualización de la Peritonitis Infecciosa Felina. Hospitales Veterinarios. 2012; 4(3):75-82. Available from: http://www.rhv.cl/index.php?option=com_docman&task=doc_download&gid=59 Fischer Y, Sauter-Louis C, Hartmann K. Diagnostic accuracy of the Rivalta test for feline infectious peritonitis. Vet Clin Pathol. 2012; 41(4):558-567. https://doi.org/10.1111/j.1939-165X.2012.00464.x. Uhart MM, Rago MV, Marull CA, Ferreyra HdV, Pereira JA. Exposure to selected pathogens in Geoffroy’s cats and domestic carnivores from central Argentina. J Wildl Dis. 2012; 48(4):899-909. https://doi.org/10.7589/2011-05-137 Alexander AB, Poirotte C, Porton IJ, Freeman KL, Rasambainarivo F, Olson KG, et al. Gastrointestinal parasites of captive and free-living lemurs and domestic carnivores in eastern Madagascar. J Zoo Wildl Med. 2016; 47(1):141-149. http://dx.doi.org/10.1638/2015-0111.1. Napolitano C, Sacristán I, Beltrán-Saavedra LF, Limachi-Quiñajo R, Poulin E. Molecular and serologic survey of pathogens in an endangered andean cat (Leopardus jacobita) of the high andes of Bolivia. J Wildl Dis. 2019; 55(1):242-245. https://doi.org/10.7589/2018-05-136. de Oliveira TES, Di Santis GW, Headley SA. Epidemiological data and a score-based study of renal, hepatic and cerebral lesions in feline infectious peritonitis. Semin Cienc Agrar. 2017; 38(5):3133-3143. http://dx.doi.org/10.5433/1679-0359.2017v38n5p3133. Furtado MM, de Ramos Filho JD, Scheffer KC, Coelho CJ, Cruz PS, Ikuta CY, et al. Serosurvey for selected viral infections in free-ranging jaguars (Panthera onca) and domestic carnivores in Brazilian Cerrado, Pantanal, and Amazon. J Wildl Dis. 2013; 49(3):510-521. https://doi.org/10.7589/2012-02-056. Filoni C, Catão-Dias JL, Cattori V, Willi B, Meli ML, Corrêa SHR, et al. Surveillance using serological and molecular methods for the detection of infectious agents in captive Brazilian neotropic and exotic felids. J Vet Diagn Invest. 2012; 24(1):166-173. https://doi.org/10.1177/1040638711407684. Furtado MM, Taniwaki SA, de Barros IN, Brandão PE, Catão-Dias JL, Cavalcanti S, et al. Molecular detection of viral agents in free-ranging and captive neotropical felids in Brazil. J Vet Diagn Invest. 2017; 29(5):660-668. https://doi.org/10.1177/1040638717720245. Castro TX, Rita de Cássia N, Fumian TM, Costa EM, Mello R, White PA, et al. Detection and molecular characterization of caliciviruses (vesivirus and norovirus) in an outbreak of acute diarrhea in kittens from Brazil. Vet J. 2015; 206(1):115-117. https://doi.org/10.1016/j.tvjl.2015.06.014. de Barros BdCV, de Castro CMO, Pereira D, Ribeiro LG, Júnior JWBD, Casseb SMM, et al. First complete genome sequence of a feline alphacoronavirus 1 strain from Brazil. Microbiol Resour Announc. 2019; 8(10):1-2. https://doi.org/10.1128/MRA.01535-18. Lauzi S, Stranieri A, Giordano A, Luzzago C, Zehender G, Paltrinieri S, et al. Origin and transmission of Feline coronavirus type I in domestic cats from Northern Italy: a phylogeographic approach. Vet Microbiol. 2020; 244:1-9. https://doi.org/10.1016/j.vetmic.2020.108667. Dea S, Roy R, Elazhary M. Coronavirus-like particles in the feces of a cat with diarrhea. Can Vet J. 1982; 23(5):153. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1790106/. Licht DS, Moen RA, Brown DP, Romanski MC, Gitzen RA. The Canada Lynx (Lynx canadensis) of Isle Royale: over-harvest, climate change, and the extirpation of an island population. Can Field-Nat. 2019;129(2):139-151. https://doi.org/10.22621/cfn.v129i2.1694. Bauer BS, Kerr ME, Sandmeyer LS, Grahn BH. Positive immunostaining for feline infectious peritonitis (FIP) in a Sphinx cat with cutaneous lesions and bilateral panuveitis. Vet Ophthalmol. 2013; 16(1):160-163. https://doi.org/10.1111/vop.12044. McKay LA, Meachem M, Snead E, Brannen T, Mutlow N, Ruelle L, et al. Prevalence and mutation analysis of the spike protein in feline enteric coronavirus and feline infectious peritonitis detected in household and shelter cats in western Canada. Can J Vet Res. 2020; 84(1):18-23. Available from: https://www.ncbi.nlm.nih.gov/pubmed/31949325. Albala A. Peritonitis infecciosa felina en Chile. Comunicación preliminar. Monogr Med Vet. 1986; 8(2). Available from: https://web.uchile.cl/vignette/monografiasveterinaria/monografiasveterinaria.uchile.cl/CDA/mon_vet_articulo/0,1412,SCID%253D8120%2526ISID%253D418%2526PRT%253D0,00.html Ochoa J, Roque A, Daza J. Colangiocarcinoma hepático en un felino y hallazgos anatomopatólogicos, y clínicos compatibles con peritonitis infecciosa felina. Rev MVZ Córdoba. 2012; 17(2):3080-3086. https://doi.org/10.21897/rmvz.245. Rodríguez JCB, Betancur AM, Salcedo LK, León SP. Caso clínico felino con peritonitis infecciosa felina (PIF) ocasionado por un coronavirus. Rev Electrón Vet. 2017; 18(7):1-9. Available from: https://www.redalyc.org/pdf/636/63652580012.pdf. Ramirez Cardona ID, Velasquez Viera MA, Ramirez Garcia R, Hernandez Lopez CA. Estudio de seroprevalencia del coronavirus felino en gatos callejeros, de albergue y caseros de la ciudad de Medellín durante el 2013. [Tesis de maestría]. Colombia: Universidad CES, 2014. https://repository.ces.edu.co/handle/10946/2128. Fletcher Uribe S, Perez Garcia J, Villegas Tabares JP. Diagnóstico de agentes infecciosos de común presentación en felinos silvestres nativos y exóticos mantenidos en cautiverio en Colombia. Colombia: Universidad CES, 2017. Available from: https://repository.ces.edu.co/handle/10946/2848. Delgado Villamizar KY. Seroprevalencia y evaluación molecular de coronavirus felino en Bucaramanga utilizando RT-PCR. [Tesis de maestría]. Colombia: Universidad Coperativa de Colombia, 2018. Available from: https://repository.ucc.edu.co/handle/20.500.12494/11667. Santana N, Reyes D, Arango D, Velandia A, Taniwaki S, de Souza S, Brandão, P. Molecular diversity of Alphacoronavirus 1 in dogs and cats in Colombia. Heliyon. 2020; 6(7):1-6. https://doi.org/10.1016/j.heliyon.2020.e04381. Teixeira BM, Hagiwara MK, Cruz J, Hosie MJ. Feline immunodeficiency virus in South America. Viruses. 2012; 4(3):383-396. https://doi.org/10.3390/v4030383. Zook B, King N, Robison R, McCombs H. Ultrastructural evidence for the viral etiology of feline infectious peritonitis. Vet Pathol. 1968; 5(1):91-95. https://doi.org/10.1177/030098586800500112. Domínguez M, Moreno I, Toraño A. Quantitation of monoclonal antibody by capture ELISA based on initial enzyme activity rate. J Immunol Methods. 2019; 474:1-6. https://doi.org/10.1016/j.jim.2019.112645. Kim Y, Mandadapu SR, Groutas WC, Chang K-O. Potent inhibition of feline coronaviruses with peptidyl compounds targeting coronavirus 3C-like protease. Antiviral Res. 2013; 97(2):161-168. https://doi.org/10.1016/j.antiviral.2012.11.005. Foley JE, Swift P, Fleer KA, Torres S, Girard YA, Johnson CK. Risk factors for exposure to feline pathogens in California mountain lions (Puma concolor). J Wildl Dis. 2013; 49(2):279-293. https://doi.org/10.7589/2012-08-206. Gaffney PM, Kennedy M, Terio K, Gardner I, Lothamer C, Coleman K, et al. Detection of feline coronavirus in cheetah (Acinonyx jubatus) feces by reverse transcription-nested polymerase chain reaction in cheetahs with variable frequency of viral shedding. J Zoo Wildl Med. 2012; 43(4):776-786. https://doi.org/10.1638/2011-0110R1.1. Polak K, Levy J, Crawford P, Leutenegger C, Moriello K. Infectious diseases in large-scale cat hoarding investigations. Vet J 2014; 201(2):189-195. https://doi.org/10.1016/j.tvjl.2014.05.020. Fish EJ, Diniz PPV, Juan Y-C, Bossong F, Collisson EW, Drechsler Y, et al. Cross-sectional quantitative RT-PCR study of feline coronavirus viremia and replication in peripheral blood of healthy shelter cats in Southern California. J Feline Med Surg. 2018; 20(4):295-301. https://doi.org/10.1177/1098612X17705227. Lickey AL, Kennedy M, Patton S, Ramsay EC. Serologic survey of domestic felids in the Petén region of Guatemala. Journal of Zoo and Wildlife Medicine. 2005; 36(1):121-123. https://doi.org/10.1638/03-059. Pereyra C, Rosadio R. Evidencias de infecciones coronavirales y retrovirales en felinos de lima metropolitana. Rev Inv Pec (IVITA). 1997;8(1):79-82. Available from: https://sisbib.unmsm.edu.pe/bvrevistas/veterinaria/v08_n1/infeccionesc.htm. Rubio A, Chavera A. Peritonitis infecciosa felina: dos casos clínicos en Lima-Perú. Rev Investig Vet Peru. 2018; 29(1):381-388. http://dx.doi.org/10.15381/rivep.v29i1.14188. Echeto OEV, Madrigal K, Admadé M, Oviedo ORV, Moreno A, Simoes D. Peritonitis infecciosa felina, gastroenteritis y colangiohepatitis parasitaria (Platinosomiasis) con colangiocarcinoma hepático: Estudio clínico y anatomopatológico de tres casos. Rev Cient (FCV-LUZ). 2005; 15(3):195-203. http://www.saber.ula.ve/handle/123456789/28308. |
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Valencia, Alida CarolinaDelgado Villamizar, KarenRuiz Sáenz, Julián2022-02-09T12:20:54Z2022-02-09T12:20:54Z2021-040122-0268doi.org/10.21897/rmvz.2041https://hdl.handle.net/20.500.12494/43883Valencia G, A., Delgado-Villamizar, K., & Ruiz-Saenz, J. (2021). Serological and molecular diagnosis of the feline coronavirus in the americas. Journal MVZ Cordoba, 26(2), e2041. https://doi.org/10.21897/rmvz.2041Since its discovery by Holzworth in 1962, the study of the Feline Coronavirus (FCoV) has have a great interest because it can affect wildlife and domestic felines. Currently 2 serotypes are known, type I is unique for felines and type II arose from a double homologous recombination with a canine coronavirus (CCoV); these also can be classified in 2 biotypes, viruses that generate mild enteric diseases (FECVs) and those that cause the feline infectious peritonitis (FIPVs). In the American continent exist different diagnostic methods that together allow the detection of the feline infectious peritonitis (FIP), but the identification of the FCoV only can be done by molecular methods. The countries that have studied this virus the most are those that have the greatest number of tools to carry out diagnostic test, such as United States, Canada and Brazil. In the present work are shown cases reports and the diagnostics methods used to identify the feline coronavirus and/or its biotypes in some countries of the American continent.http://scienti.colciencias.gov.co:8081/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000153095https://orcid.org/0000-0002-1447-1458https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000000695julian.ruizs@campusucc.edu.cohttps://scholar.google.com/citations?user=o3Y7mZwAAAAJ&hl=ese2041.Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, BucaramangaUniversidad de CordobaMedicina veterinaria y zootecniaBucaramangaDiagnosticFeline CoronavirusFIPVFCoVSerological and molecular diagnosis of the feline coronavirus in the americasArtículos Científicoshttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribución – No comercial – Compartir igualinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf226 p.Lemmermeyer T, Lamp B, Schneider R, Ziebuhr J, Tekes G, Thiel H-J. Characterization of monoclonal antibodies against feline coronavirus accessory protein 7b. Vet Microbiol. 2016; 184:11-19. https://doi.org/10.1016/j.vetmic.2015.12.009.Kipar A, Meli M. Feline infectious peritonitis: still an enigma? Vet Pathol. 2014; 51(2):505-526. https://doi.org/10.1177/0300985814522077.Jaimes JA, Whittaker GR. Feline coronavirus: insights into viral pathogenesis based on the spike protein structure and function. Virology. 2018; 517:108-121.Chang H-W, de Groot RJ, Egberink HF, Rottier PJ. Feline infectious peritonitis: insights into feline coronavirus pathobiogenesis and epidemiology based on genetic analysis of the viral 3c gene. J Gen Virol. 2010; 91(2):415-420. https://doi.org/10.1099/vir.0.016485-0.Shiba N, Maeda K, Kato H, Mochizuki M, Iwata H. Differentiation of feline coronavirus type I and II infections by virus neutralization test. Vet microbiol. 2007; 124(3-4):348-352. https://doi.org/10.1016/j.vetmic.2007.04.031.Tekes G, Thiel H-J. Feline coronaviruses: pathogenesis of feline infectious peritonitis. Advances in virus research. Elsevier. 2016; 96:193-218. https://doi.org/10.1016/bs.aivir.2016.08.002.Desmarets LM, Vermeulen BL, Theuns S, Conceição-Neto N, Zeller M, Roukaerts ID, et al. Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures. Sci Rep. 2016; 6(1):1-11. https://doi.org/10.1038/srep20022.Kim Y, Liu H, Kankanamalage ACG, Weerasekara S, Hua DH, Groutas WC, et al. Reversal of the progression of fatal coronavirus infection in cats by a broad-spectrum coronavirus protease inhibitor. PLoS Pathog. 2016; 12(3):1-18. https://doi.org/10.1371/journal.ppat.1005650.Licitra BN, Millet JK, Regan AD, Hamilton BS, Rinaldi VD, Duhamel GE, et al. Mutation in spike protein cleavage site and pathogenesis of feline coronavirus. Emerg Infect Dis. 2013; 19(7):1066-1073. http://dx.doi.org/10.3201/eid1907.121094.Sharif S, Arshad SS, Hair-Bejo M, Omar AR, Zeenathul NA, Alazawy A. Diagnostic methods for feline coronavirus: a review. Vet Med Int. 2010; 2010:1-8. https://doi.org/10.4061/2010/809480.Hartmann K. Feline infectious peritonitis. Vet Clin Small Anim. 2005; 35(1):39-79. https://doi.org/10.1016/j.cvsm.2004.10.011.Addie DD. Feline infectious peritonitis: answers to frequently asked questions concerning FIP and coronavirus. VNJ. 2019; 34(8):201-206. https://doi.org/10.1080/17415349.2019.1629366.Tasker S. Diagnosis of feline infectious peritonitis: Update on evidence supporting available tests. J Feline Med Surg. 2018; 20(3):228-243. https://doi.org/10.1177/1098612X18758592.Takano T, Hohdatsu T. Serological diagnosis of feline coronavirus infection by immunochromatographic test. Coronaviruses: Springer. 2015; 1282:33-39. https://doi.org/10.1007/978-1-4939-2438-7_3.Hora A, Tonietti P, Taniwaki S, Asano K, Maiorka P, Richtzenhain L, et al. Feline coronavirus 3C protein: a candidate for a virulence marker? BioMed Res Int. 2016; 9(8):1-9. http://dx.doi.org/10.1155/2016/8560691.Pedersen NC. An update on feline infectious peritonitis: diagnostics and therapeutics. Vet J. 2014; 201(2):133-141. https://doi.org/10.1016/j.tvjl.2014.04.016.Prieto M, Acuña A. Actualización de la Peritonitis Infecciosa Felina. Hospitales Veterinarios. 2012; 4(3):75-82. Available from: http://www.rhv.cl/index.php?option=com_docman&task=doc_download&gid=59Fischer Y, Sauter-Louis C, Hartmann K. Diagnostic accuracy of the Rivalta test for feline infectious peritonitis. Vet Clin Pathol. 2012; 41(4):558-567. https://doi.org/10.1111/j.1939-165X.2012.00464.x.Uhart MM, Rago MV, Marull CA, Ferreyra HdV, Pereira JA. Exposure to selected pathogens in Geoffroy’s cats and domestic carnivores from central Argentina. J Wildl Dis. 2012; 48(4):899-909. https://doi.org/10.7589/2011-05-137Alexander AB, Poirotte C, Porton IJ, Freeman KL, Rasambainarivo F, Olson KG, et al. Gastrointestinal parasites of captive and free-living lemurs and domestic carnivores in eastern Madagascar. J Zoo Wildl Med. 2016; 47(1):141-149. http://dx.doi.org/10.1638/2015-0111.1.Napolitano C, Sacristán I, Beltrán-Saavedra LF, Limachi-Quiñajo R, Poulin E. Molecular and serologic survey of pathogens in an endangered andean cat (Leopardus jacobita) of the high andes of Bolivia. J Wildl Dis. 2019; 55(1):242-245. https://doi.org/10.7589/2018-05-136.de Oliveira TES, Di Santis GW, Headley SA. Epidemiological data and a score-based study of renal, hepatic and cerebral lesions in feline infectious peritonitis. Semin Cienc Agrar. 2017; 38(5):3133-3143. http://dx.doi.org/10.5433/1679-0359.2017v38n5p3133.Furtado MM, de Ramos Filho JD, Scheffer KC, Coelho CJ, Cruz PS, Ikuta CY, et al. Serosurvey for selected viral infections in free-ranging jaguars (Panthera onca) and domestic carnivores in Brazilian Cerrado, Pantanal, and Amazon. J Wildl Dis. 2013; 49(3):510-521. https://doi.org/10.7589/2012-02-056.Filoni C, Catão-Dias JL, Cattori V, Willi B, Meli ML, Corrêa SHR, et al. Surveillance using serological and molecular methods for the detection of infectious agents in captive Brazilian neotropic and exotic felids. J Vet Diagn Invest. 2012; 24(1):166-173. https://doi.org/10.1177/1040638711407684.Furtado MM, Taniwaki SA, de Barros IN, Brandão PE, Catão-Dias JL, Cavalcanti S, et al. Molecular detection of viral agents in free-ranging and captive neotropical felids in Brazil. J Vet Diagn Invest. 2017; 29(5):660-668. https://doi.org/10.1177/1040638717720245.Castro TX, Rita de Cássia N, Fumian TM, Costa EM, Mello R, White PA, et al. Detection and molecular characterization of caliciviruses (vesivirus and norovirus) in an outbreak of acute diarrhea in kittens from Brazil. Vet J. 2015; 206(1):115-117. https://doi.org/10.1016/j.tvjl.2015.06.014.de Barros BdCV, de Castro CMO, Pereira D, Ribeiro LG, Júnior JWBD, Casseb SMM, et al. First complete genome sequence of a feline alphacoronavirus 1 strain from Brazil. Microbiol Resour Announc. 2019; 8(10):1-2. https://doi.org/10.1128/MRA.01535-18.Lauzi S, Stranieri A, Giordano A, Luzzago C, Zehender G, Paltrinieri S, et al. Origin and transmission of Feline coronavirus type I in domestic cats from Northern Italy: a phylogeographic approach. Vet Microbiol. 2020; 244:1-9. https://doi.org/10.1016/j.vetmic.2020.108667.Dea S, Roy R, Elazhary M. Coronavirus-like particles in the feces of a cat with diarrhea. Can Vet J. 1982; 23(5):153. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1790106/.Licht DS, Moen RA, Brown DP, Romanski MC, Gitzen RA. The Canada Lynx (Lynx canadensis) of Isle Royale: over-harvest, climate change, and the extirpation of an island population. Can Field-Nat. 2019;129(2):139-151. https://doi.org/10.22621/cfn.v129i2.1694.Bauer BS, Kerr ME, Sandmeyer LS, Grahn BH. Positive immunostaining for feline infectious peritonitis (FIP) in a Sphinx cat with cutaneous lesions and bilateral panuveitis. Vet Ophthalmol. 2013; 16(1):160-163. https://doi.org/10.1111/vop.12044.McKay LA, Meachem M, Snead E, Brannen T, Mutlow N, Ruelle L, et al. Prevalence and mutation analysis of the spike protein in feline enteric coronavirus and feline infectious peritonitis detected in household and shelter cats in western Canada. Can J Vet Res. 2020; 84(1):18-23. Available from: https://www.ncbi.nlm.nih.gov/pubmed/31949325.Albala A. Peritonitis infecciosa felina en Chile. Comunicación preliminar. Monogr Med Vet. 1986; 8(2). Available from: https://web.uchile.cl/vignette/monografiasveterinaria/monografiasveterinaria.uchile.cl/CDA/mon_vet_articulo/0,1412,SCID%253D8120%2526ISID%253D418%2526PRT%253D0,00.htmlOchoa J, Roque A, Daza J. Colangiocarcinoma hepático en un felino y hallazgos anatomopatólogicos, y clínicos compatibles con peritonitis infecciosa felina. Rev MVZ Córdoba. 2012; 17(2):3080-3086. https://doi.org/10.21897/rmvz.245.Rodríguez JCB, Betancur AM, Salcedo LK, León SP. Caso clínico felino con peritonitis infecciosa felina (PIF) ocasionado por un coronavirus. Rev Electrón Vet. 2017; 18(7):1-9. Available from: https://www.redalyc.org/pdf/636/63652580012.pdf.Ramirez Cardona ID, Velasquez Viera MA, Ramirez Garcia R, Hernandez Lopez CA. Estudio de seroprevalencia del coronavirus felino en gatos callejeros, de albergue y caseros de la ciudad de Medellín durante el 2013. [Tesis de maestría]. Colombia: Universidad CES, 2014. https://repository.ces.edu.co/handle/10946/2128.Fletcher Uribe S, Perez Garcia J, Villegas Tabares JP. Diagnóstico de agentes infecciosos de común presentación en felinos silvestres nativos y exóticos mantenidos en cautiverio en Colombia. Colombia: Universidad CES, 2017. Available from: https://repository.ces.edu.co/handle/10946/2848.Delgado Villamizar KY. Seroprevalencia y evaluación molecular de coronavirus felino en Bucaramanga utilizando RT-PCR. [Tesis de maestría]. Colombia: Universidad Coperativa de Colombia, 2018. Available from: https://repository.ucc.edu.co/handle/20.500.12494/11667.Santana N, Reyes D, Arango D, Velandia A, Taniwaki S, de Souza S, Brandão, P. Molecular diversity of Alphacoronavirus 1 in dogs and cats in Colombia. Heliyon. 2020; 6(7):1-6. https://doi.org/10.1016/j.heliyon.2020.e04381.Teixeira BM, Hagiwara MK, Cruz J, Hosie MJ. Feline immunodeficiency virus in South America. Viruses. 2012; 4(3):383-396. https://doi.org/10.3390/v4030383.Zook B, King N, Robison R, McCombs H. Ultrastructural evidence for the viral etiology of feline infectious peritonitis. Vet Pathol. 1968; 5(1):91-95. https://doi.org/10.1177/030098586800500112.Domínguez M, Moreno I, Toraño A. Quantitation of monoclonal antibody by capture ELISA based on initial enzyme activity rate. J Immunol Methods. 2019; 474:1-6. https://doi.org/10.1016/j.jim.2019.112645.Kim Y, Mandadapu SR, Groutas WC, Chang K-O. Potent inhibition of feline coronaviruses with peptidyl compounds targeting coronavirus 3C-like protease. Antiviral Res. 2013; 97(2):161-168. https://doi.org/10.1016/j.antiviral.2012.11.005.Foley JE, Swift P, Fleer KA, Torres S, Girard YA, Johnson CK. Risk factors for exposure to feline pathogens in California mountain lions (Puma concolor). J Wildl Dis. 2013; 49(2):279-293. https://doi.org/10.7589/2012-08-206.Gaffney PM, Kennedy M, Terio K, Gardner I, Lothamer C, Coleman K, et al. Detection of feline coronavirus in cheetah (Acinonyx jubatus) feces by reverse transcription-nested polymerase chain reaction in cheetahs with variable frequency of viral shedding. J Zoo Wildl Med. 2012; 43(4):776-786. https://doi.org/10.1638/2011-0110R1.1.Polak K, Levy J, Crawford P, Leutenegger C, Moriello K. Infectious diseases in large-scale cat hoarding investigations. Vet J 2014; 201(2):189-195. https://doi.org/10.1016/j.tvjl.2014.05.020.Fish EJ, Diniz PPV, Juan Y-C, Bossong F, Collisson EW, Drechsler Y, et al. Cross-sectional quantitative RT-PCR study of feline coronavirus viremia and replication in peripheral blood of healthy shelter cats in Southern California. J Feline Med Surg. 2018; 20(4):295-301. https://doi.org/10.1177/1098612X17705227.Lickey AL, Kennedy M, Patton S, Ramsay EC. Serologic survey of domestic felids in the Petén region of Guatemala. Journal of Zoo and Wildlife Medicine. 2005; 36(1):121-123. https://doi.org/10.1638/03-059.Pereyra C, Rosadio R. Evidencias de infecciones coronavirales y retrovirales en felinos de lima metropolitana. Rev Inv Pec (IVITA). 1997;8(1):79-82. Available from: https://sisbib.unmsm.edu.pe/bvrevistas/veterinaria/v08_n1/infeccionesc.htm.Rubio A, Chavera A. Peritonitis infecciosa felina: dos casos clínicos en Lima-Perú. Rev Investig Vet Peru. 2018; 29(1):381-388. http://dx.doi.org/10.15381/rivep.v29i1.14188.Echeto OEV, Madrigal K, Admadé M, Oviedo ORV, Moreno A, Simoes D. Peritonitis infecciosa felina, gastroenteritis y colangiohepatitis parasitaria (Platinosomiasis) con colangiocarcinoma hepático: Estudio clínico y anatomopatológico de tres casos. Rev Cient (FCV-LUZ). 2005; 15(3):195-203. http://www.saber.ula.ve/handle/123456789/28308.PublicationORIGINAL2022_serological_molecular_diagnosis-FormatoLicenciaUso.pdf2022_serological_molecular_diagnosis-FormatoLicenciaUso.pdfapplication/pdf207210https://repository.ucc.edu.co/bitstreams/eb997f5b-addc-4b8f-ac9d-e833bee2ab86/download255cf067c2c70bf4bcb4f264640646ccMD512022_serological_molecular_diagnosis.pdf2022_serological_molecular_diagnosis.pdfapplication/pdf679027https://repository.ucc.edu.co/bitstreams/ac5d38fc-f237-481a-887c-7335d35af70b/downloadfcf48d183095a403424026766086e749MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-84334https://repository.ucc.edu.co/bitstreams/fcd43bdd-e5fc-41d5-a896-6ff3c1c34b0b/download3bce4f7ab09dfc588f126e1e36e98a45MD53THUMBNAIL2022_serological_molecular_diagnosis-FormatoLicenciaUso.pdf.jpg2022_serological_molecular_diagnosis-FormatoLicenciaUso.pdf.jpgGenerated 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12:27:16.037restrictedhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de 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