Partial characterization of venom from the colombian spider Phoneutria Boliviensis (Aranae:Ctenidae)
We report on the first studies on the characterization of venom from Phoneutria boliviensis (Aranae:Ctenidae) (F. O. Pickard-Cambridge, 1897), done with Colombian species.
- Autores:
-
Estrada Gómez, Sebastián
Vargas Muñoz, Leidy Johana
Lanchero, Paula
Segura Latorre, César
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2015
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/1332
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/1332
- Palabra clave:
- Phoneutria boliviensis
Proteomic analysis
Insecticidal activity
Phospholipases
- Rights
- openAccess
- License
- Licencia CC
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| dc.title.spa.fl_str_mv |
Partial characterization of venom from the colombian spider Phoneutria Boliviensis (Aranae:Ctenidae) |
| title |
Partial characterization of venom from the colombian spider Phoneutria Boliviensis (Aranae:Ctenidae) |
| spellingShingle |
Partial characterization of venom from the colombian spider Phoneutria Boliviensis (Aranae:Ctenidae) Phoneutria boliviensis Proteomic analysis Insecticidal activity Phospholipases |
| title_short |
Partial characterization of venom from the colombian spider Phoneutria Boliviensis (Aranae:Ctenidae) |
| title_full |
Partial characterization of venom from the colombian spider Phoneutria Boliviensis (Aranae:Ctenidae) |
| title_fullStr |
Partial characterization of venom from the colombian spider Phoneutria Boliviensis (Aranae:Ctenidae) |
| title_full_unstemmed |
Partial characterization of venom from the colombian spider Phoneutria Boliviensis (Aranae:Ctenidae) |
| title_sort |
Partial characterization of venom from the colombian spider Phoneutria Boliviensis (Aranae:Ctenidae) |
| dc.creator.fl_str_mv |
Estrada Gómez, Sebastián Vargas Muñoz, Leidy Johana Lanchero, Paula Segura Latorre, César |
| dc.contributor.advisor.none.fl_str_mv |
Vargas Muñoz, Leidy Johana |
| dc.contributor.author.none.fl_str_mv |
Estrada Gómez, Sebastián Vargas Muñoz, Leidy Johana Lanchero, Paula Segura Latorre, César |
| dc.subject.spa.fl_str_mv |
Phoneutria boliviensis Proteomic analysis Insecticidal activity Phospholipases |
| topic |
Phoneutria boliviensis Proteomic analysis Insecticidal activity Phospholipases |
| description |
We report on the first studies on the characterization of venom from Phoneutria boliviensis (Aranae:Ctenidae) (F. O. Pickard-Cambridge, 1897), done with Colombian species. |
| publishDate |
2015 |
| dc.date.issued.none.fl_str_mv |
2015-07-31 |
| dc.date.accessioned.none.fl_str_mv |
2017-08-23T23:41:08Z |
| dc.date.available.none.fl_str_mv |
2017-08-23T23:41:08Z |
| dc.type.none.fl_str_mv |
Artículo |
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http://purl.org/coar/resource_type/c_6501 |
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https://hdl.handle.net/20.500.12494/1332 |
| dc.identifier.bibliographicCitation.spa.fl_str_mv |
Estrada Gómez, S., Vargas Muñoz, L. J., Lanchero, P. & Segura Latorre, C. (2015). Partial Characterization of Venom from the Colombian Spider Phoneutria Boliviensis (Aranae:Ctenidae). Toxins, 7, 2872-2887; doi:10.3390/toxins7082872 |
| url |
https://hdl.handle.net/20.500.12494/1332 |
| identifier_str_mv |
Estrada Gómez, S., Vargas Muñoz, L. J., Lanchero, P. & Segura Latorre, C. (2015). Partial Characterization of Venom from the Colombian Spider Phoneutria Boliviensis (Aranae:Ctenidae). Toxins, 7, 2872-2887; doi:10.3390/toxins7082872 |
| dc.relation.isversionof.spa.fl_str_mv |
http://www.mdpi.com/2072-6651/7/8/2872 |
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Licencia CC |
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info:eu-repo/semantics/openAccess |
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http://purl.org/coar/access_right/c_abf2 |
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Licencia CC http://purl.org/coar/access_right/c_abf2 |
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openAccess |
| dc.publisher.spa.fl_str_mv |
Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Medellín y Envigado, Colombia, 00000 |
| dc.publisher.program.spa.fl_str_mv |
Medicina |
| dc.publisher.place.spa.fl_str_mv |
Medellín |
| institution |
Universidad Cooperativa de Colombia |
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Vargas Muñoz, Leidy JohanaEstrada Gómez, SebastiánVargas Muñoz, Leidy JohanaLanchero, PaulaSegura Latorre, César2017-08-23T23:41:08Z2017-08-23T23:41:08Z2015-07-31https://hdl.handle.net/20.500.12494/1332Estrada Gómez, S., Vargas Muñoz, L. J., Lanchero, P. & Segura Latorre, C. (2015). Partial Characterization of Venom from the Colombian Spider Phoneutria Boliviensis (Aranae:Ctenidae). Toxins, 7, 2872-2887; doi:10.3390/toxins7082872We report on the first studies on the characterization of venom from Phoneutria boliviensis (Aranae:Ctenidae) (F. O. Pickard-Cambridge, 1897), done with Colombian species.We report on the first studies on the characterization of venom from Phoneutria boliviensis (Aranae:Ctenidae) (F. O. Pickard-Cambridge, 1897), done with Colombian species. After the electrostimulation extraction process, the venom showed physicochemical properties corresponding to a colorless and water-soluble liquid with a density of 0.86 mg/mL and 87% aqueous content. P. boliviensis venom and RP-HPLC fractions showed hemolytic activity and hydrolyzed the synthetic substrate 4-nitro-3-octanoyloxy-benzoic acid, indicating the presence of phospholipases A2 enzymes. The electrophoretic profile showed an important protein content with molecular masses below 14 kDa, and differences between male and female protein content were also revealed. The RP-HPLC venom profile exposes differences between males and female content consistent with the electrophoretic profile. Five fractions collected from the RP-HPLC displayed significant larvicidal activity. Mass analysis indicates the presence of peptides ranging from 1047.71 to 3278.07 Da. Two peptides, Ctenitoxin-Pb48 and Ctenitoxin-Pb53, were partially identified using HPLC-nESI-MS/MS, which showed a high homology with other Ctenitoxins (family Tx3) from Phoneutria nigriventer, Phoneutria keyserlingi and Phoneutria reidyi affecting voltage-gated calcium receptors (Cav 1, 2.1, 2.2 and 2.3) and NMDA-glutamate receptors.sebastian.estrada@udea.edu.coleidy.vargasmu@campusucc.edu.coUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Medellín y Envigado, Colombia, 00000MedicinaMedellínhttp://www.mdpi.com/2072-6651/7/8/2872Phoneutria boliviensisProteomic analysisInsecticidal activityPhospholipasesPartial characterization of venom from the colombian spider Phoneutria Boliviensis (Aranae:Ctenidae)Artículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionLicencia CCinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationORIGINAL2015 Partial Characterization of Venom from the Colombian Spider Phoneutria Boliviensis (AranaeCtenidae).pdf2015 Partial Characterization of Venom from the Colombian Spider Phoneutria Boliviensis (AranaeCtenidae).pdfapplication/pdf670682https://repository.ucc.edu.co/bitstreams/47cb5deb-cc4a-42c8-90ea-9561bf932683/downloade87eb5f19bd7d5f32d0bc3747752952eMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-84553https://repository.ucc.edu.co/bitstreams/9b9adf01-8be1-439e-8d93-52a5d0cc61f8/downloadcb8f4d9b72d863ad6737f22af442cc82MD52THUMBNAIL2015 Partial Characterization of Venom from the Colombian Spider Phoneutria Boliviensis (AranaeCtenidae).pdf.jpg2015 Partial Characterization of Venom from the Colombian Spider Phoneutria Boliviensis (AranaeCtenidae).pdf.jpgIM 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