Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study
The Colombian rattlesnake Crotalus durissus cumanensis is distributed in three geographic zones of the country: the Atlantic Coast, the upper valley of the Magdalena River, and the eastern plains of the Colombian Orinoquía. Its venom induces neurological symptoms, such as eyelid ptosis, myasthenic f...
- Autores:
-
Quintana Castillo, Juan Carlos
Vargas Muñoz, Leidy Johana
Segura Latorre, César
Estrada Gómez, Sebastián
Bueno Sánchez, Julio César
Alarcón, Juan Carlos
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/15917
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/15917
- Palabra clave:
- C. d. cumanensis venom
antivenom
HPLC-nESI-MS/MS
peptide identification
- Rights
- openAccess
- License
- Atribución – No comercial – Sin Derivar
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Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study |
| title |
Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study |
| spellingShingle |
Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study C. d. cumanensis venom antivenom HPLC-nESI-MS/MS peptide identification |
| title_short |
Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study |
| title_full |
Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study |
| title_fullStr |
Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study |
| title_full_unstemmed |
Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study |
| title_sort |
Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study |
| dc.creator.fl_str_mv |
Quintana Castillo, Juan Carlos Vargas Muñoz, Leidy Johana Segura Latorre, César Estrada Gómez, Sebastián Bueno Sánchez, Julio César Alarcón, Juan Carlos |
| dc.contributor.author.none.fl_str_mv |
Quintana Castillo, Juan Carlos Vargas Muñoz, Leidy Johana Segura Latorre, César Estrada Gómez, Sebastián Bueno Sánchez, Julio César Alarcón, Juan Carlos |
| dc.subject.spa.fl_str_mv |
C. d. cumanensis venom antivenom HPLC-nESI-MS/MS peptide identification |
| topic |
C. d. cumanensis venom antivenom HPLC-nESI-MS/MS peptide identification |
| description |
The Colombian rattlesnake Crotalus durissus cumanensis is distributed in three geographic zones of the country: the Atlantic Coast, the upper valley of the Magdalena River, and the eastern plains of the Colombian Orinoquía. Its venom induces neurological symptoms, such as eyelid ptosis, myasthenic facies, and paralysis of the respiratory muscles, which can lead to death. Identification and analysis of C. d. cumanensis showed nine groups of proteins responsible for the neurotoxic effect, of which the crotoxin complex was the most abundant (64.71%). Immunorecognition tests of C. d. cumanensis showed that the use of a commercial antivenom manufactured in Mexico resulted in immunoreactivity. |
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2018 |
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2018-02-17 |
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2020-01-15T15:24:30Z |
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2020-01-15T15:24:30Z |
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Artículo |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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2072-6651 |
| dc.identifier.uri.spa.fl_str_mv |
10.3390/toxins10020085 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12494/15917 |
| dc.identifier.bibliographicCitation.spa.fl_str_mv |
Quintana Castillo, J.C., Vargas, L.J., Segura, C., Estrada Gómez, S., Bueno Sánchez, J.C. y Alarcón, J.C. (2018). Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study. Toxins 2018, 10, 85. Recuperado de: |
| identifier_str_mv |
2072-6651 10.3390/toxins10020085 Quintana Castillo, J.C., Vargas, L.J., Segura, C., Estrada Gómez, S., Bueno Sánchez, J.C. y Alarcón, J.C. (2018). Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study. Toxins 2018, 10, 85. Recuperado de: |
| url |
https://hdl.handle.net/20.500.12494/15917 |
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https://www.mdpi.com/2072-6651/10/2/85 |
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Toxins |
| dc.relation.references.spa.fl_str_mv |
Campbell, J.A.; Lamar,W. The Venomous Reptiles of theWestern Hemisphere; Cornell University Press: New York, NY, USA, 2004; Volume II, p. 870. Klauber, L.M. Rattlesnakes: Their Habits, Life Histories, and Influence on Mankind; Zoological Society of San Diego: San Diego, CA, USA, 1972. Bucaretchi, F.; Herrera, S.R.; Hyslop, S.; Baracat, E.C.; Vieira, R.J. Snakebites by Crotalus durissus ssp. in children in Campinas, Sao Paulo, Brazil. Rev. Inst. Med. Trop. São Paulo 2002, 44, 133–138. [CrossRef] [PubMed] Oshima-Franco, Y.; Hyslop, S.; Prado-Franceschi, J.; Cruz-Hofling, M.A.; Rodrigues-Simioni, L. Neutralizing capacity of antisera raised in horses and rabbits against Crotalus durissus terrificus (South American rattlesnake) venom and its main toxin, crotoxin. Toxicon 1999, 37, 1341–1357. [CrossRef] Bon, C. Multicomponent neurotoxic phospholipases A2. In Venom Phospholipase A2 Enzymes: Structure, Function and Mechanism;Wiley: Chichester, UK, 1997; pp. 269–285.A2. In Venom Phospholipase A2 Enzymes: Structure, Function and Mechanism; Wiley: Chichester, UK, 1997; pp. 269–285 Sampaio, S.C.; Hyslop, S.; Fontes, M.R.; Prado-Franceschi, J.; Zambelli, V.O.; Magro, A.J.; Brigatte, P.; Gutierrez, V.P.; Cury, Y. Crotoxin: Novel activities for a classic beta-neurotoxin. Toxicon 2010, 55, 1045–1060. [CrossRef] [PubMed] Azevedo-Marques, M.M.; Cupo, P.; Coimbra, T.M.; Hering, S.E.; Rossi, M.A.; Laure, C.J. Myonecrosis, myoglobinuria and acute renal failure induced by South American rattlesnake (Crotalus durissus terrificus) envenomation in Brazil. Toxicon 1985, 23, 631–636. [CrossRef] Martins, A.M.; Toyama, M.H.; Havt, A.; Novello, J.C.; Marangoni, S.; Fonteles, M.C.; Monteiro, H.S. Determination of Crotalus durissus cascavella venom components that induce renal toxicity in isolated rat kidneys. Toxicon 2002, 40, 1165–1171. [CrossRef] Cupo, P.; Azevedo-Marques, M.M.; Hering, S.E. Clinical and laboratory features of South American rattlesnake (Crotalus durissus terrificus) envenomation in children. Trans. R. Soc. Trop. Med. Hyg. 1988, 82, 924–929. [CrossRef] De Rezende, N.A.; Torres, F.M.; Dias, M.B.; Campolina, D.; Chavez-Olortegui, C.; Amaral, C.F. South American rattlesnake bite (Crotalus durissus sp.) without envenoming: Insights on diagnosis and treatment. Toxicon 1998, 36, 2029–2032. [CrossRef] Jorge, M.T.; Ribeiro, L.A. [the epidemiology and clinical picture of an accidental bite by the South American rattlesnake (Crotalus durissus)]. Rev. Inst. Med. Trop. São Paulo 1992, 34, 347–354. [CrossRef] [PubMed] Pinho, F.M.; Zanetta, D.M.; Burdmann, E.A. Acute renal failure after Crotalus durissus snakebite: A prospective survey on 100 patients. Kidney Int. 2005, 67, 659–667. [CrossRef] [PubMed] Pinho, F.O.; Zanetta, D.M.T.; Burdmann, E.A. Risk factors and prevalence of acute renal failure (ARF) after Crotalus snakebite (CSB): A prospective survey on 100 consecutive patients. J. Am. Soc. Nephrol. 2003, 14, 511a–512a. Sano-Martins, I.S.; Tomy, S.C.; Campolina, D.; Dias, M.B.; de Castro, S.C.; de Sousa-e-Silva, M.C.; Amaral, C.F.; Rezende, N.A.; Kamiguti, A.S.; Warrell, D.A.; et al. Coagulopathy following lethal and non-lethal envenoming of humans by the South American rattlesnake (Crotalus durissus) in Brazil. QJM 2001, 94, 551–559. [CrossRef] [PubMed] Gutierrez, J.M.; Leon, G.; Rojas, G.; Lomonte, B.; Rucavado, A.; Chaves, F. Neutralization of local tissue damage induced by Bothrops asper (terciopelo) snake venom. Toxicon 1998, 36, 1529–1538. [CrossRef] Leon, G.; Valverde, J.M.; Rojas, G.; Lomonte, B.; Gutierrez, J.M. Comparative study on the ability of IgG and Fab sheep antivenoms to neutralize local hemorrhage, edema and myonecrosis induced by Bothrops asper (terciopelo) snake venom. Toxicon 2000, 38, 233–244. [CrossRef] Theakston, R.D.G. The Kinetics of snakebite envenoming and therapy. In Proceedings of the 1st International Congress on Envenomations and their Treatments, Paris, France, 7–9 June 1995; p. 69. Sasa, M. Diet and snake venom evolution: Can local selection alone explain intraspecific venom variation? Toxicon 1999, 37, 249–252, author reply 253–260. [PubMed] Saldarriaga, M.M.; Otero, R.; Nunez, V.; Toro, M.F.; Diaz, A.; Gutierrez, J.M. Ontogenetic variability of Bothrops atrox and Bothrops asper snake venoms from Colombia. Toxicon 2003, 42, 405–411. [CrossRef] 20. Menezes, M.C.; Furtado, M.F.; Travaglia-Cardoso, S.R.; Camargo, A.C.; Serrano, S.M. Sex-based individual variation of snake venom proteome among eighteen Bothrops jararaca siblings. Toxicon 2006, 47, 304–312. [CrossRef] [PubMed] Antunez, J.; Fernandez, J.; Lomonte, B.; Angulo, Y.; Sanz, L.; Perez, A.; Calvete, J.J.; Gutierrez, J.M. Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activities. J. Venom. Res. 2010, 1, 8–17. [PubMed] Gutierrez, J.M.; dos Santos, M.C.; Furtado Mde, F.; Rojas, G. Biochemical and pharmacological similarities between the venoms of newborn Crotalus durissus durissus and adult Crotalus durissus terrificus rattlesnakes. Toxicon 1991, 29, 1273–1277. [CrossRef] Currier, R.B.; Harrison, R.A.; Rowley, P.D.; Laing, G.D.;Wagstaff, S.C. Intra-specific variation in venom of the african puff adder (bitis arietans): Differential expression and activity of snake venom metalloproteinases (SVMPS). Toxicon 2010, 55, 864–873. [CrossRef] [PubMed] Tun, P.; Nu Nu, L.; Aye Aye, M.; Kyi May, H.; Khin Aung, C. Biochemical and biological properties of the venom from Russell’s viper (Daboia russelli siamensis) of varying ages. Toxicon 1995, 33, 817–821. [CrossRef] Rael, E.D.; Rivas, J.Z.; Chen, T.; Maddux, N.; Huizar, E.; Lieb, C.S. Differences in fibrinolysis and complement inactivation by venom from different northern blacktailed rattlesnakes (Crotalus molossus molossus). Toxicon 1997, 35, 505–513. [CrossRef] Ogawa, T.; Nakashima, K.; Nobuhisa, I.; Deshimaru, M.; Shimohigashi, Y.; Fukumaki, Y.; Sakaki, Y.; Hattori, S.; Ohno, M. Accelerated evolution of snake venom phospholipase A2 isozymes for acquisition of diverse physiological functions. Toxicon 1996, 34, 1229–1236. [CrossRef] Monteiro, R.Q.; Carlini, C.R.; Guimaraes, J.A.; Bon, C.; Zingali, R.B. Distinct bothrojaracin isoforms produced by individual jararaca (Bothrops jararaca) snakes. Toxicon 1997, 35, 649–657. [CrossRef] Chippaux, J.P.; Williams, V.; White, J. Snake venom variability: Methods of study, results and interpretation. Toxicon 1991, 29, 1279–1303. [CrossRef] Boldrini-Franca, J.; Correa-Netto, C.; Silva, M.M.; Rodrigues, R.S.; De La Torre, P.; Perez, A.; Soares, A.M.; Zingali, R.B.; Nogueira, R.A.; Rodrigues, V.M.; et al. Snake venomics and antivenomics of Crotalus durissus subspecies from Brazil: Assessment of geographic variation and its implication on snakebite management. J. Proteom. 2010, 73, 1758–1776. [CrossRef] [PubMed] Markland, F.S. Snake venoms and the hemostatic system. Toxicon 1998, 36, 1749–1800. [CrossRef] Calvete, J.J.; Sanz, L.; Cid, P.; de la Torre, P.; Flores-Diaz, M.; Dos Santos, M.C.; Borges, A.; Bremo, A.; Angulo, Y.; Lomonte, B.; et al. Snake venomics of the central american rattlesnake crotalus simus and the South American Crotalus durissus complex points to neurotoxicity as an adaptive paedomorphic trend along Crotalus dispersal in South America. J. Proteom. Res. 2010, 9, 528–544. [CrossRef] [PubMed] Saravia, P.; Rojas, E.; Arce, V.; Guevara, C.; López, J.C.; Chaves, E.; Velásquez, R.; Rojas, G.; Gutiérrez, J.M. Geographic and ontogenic variability in the venom of the neotropical rattlesnake Crotalus durissus: Pathophysiological and therapeutic implications. Rev. Biol. Trop. 2002, 50, 337–346. [PubMed] Céspedes, N.; Castro, F.; Jiménez, E.; Montealegre, L.; Castellanos, A.; Cañas, C.; Arévalo-Herrera, M.; Herrera, S. Biochemical comparison of venoms from young colombian Crotalus durissus cumanensis and their parents. J. Venom. Anim. Toxins Incl. Trop. Dis. 2010, 16, 268–284. [CrossRef] Otero-Patino, R.; Segura, A.; Herrera, M.; Angulo, Y.; Leon, G.; Gutierrez, J.M.; Barona, J.; Estrada, S.; Pereanez, A.; Quintana, J.C.; et al. Comparative study of the efficacy and safety of two polyvalent, caprylic acid fractionated [IgG and F(ab’)2] antivenoms, in Bothrops asper bites in Colombia. Toxicon 2012, 59, 344–355. [CrossRef] [PubMed] Patino, A.C.; Lopez, J.; Aristizabal, M.; Quintana, J.C.; Benjumea, D. [evaluation of the inhibitory effect of extracts from leaves of Renealmia alpinia rottb. Maas (Zingiberaceae) on the venom of Bothrops asper (mapana)]. Biomedica 2012, 32, 365–374. [PubMed] Angulo, Y.; Escolano, J.; Lomonte, B.; Gutierrez, J.M.; Sanz, L.; Calvete, J.J. Snake venomics of central american pitvipers: Clues for rationalizing the distinct envenomation profiles of Atropoides nummifer and Atropoides picadoi. J. Proteom. Res. 2008, 7, 708–719. [CrossRef] [PubMed] Valente, R.H.; Guimaraes, P.R.; Junqueira, M.; Neves-Ferreira, A.G.; Soares, M.R.; Chapeaurouge, A.; Trugilho, M.R.; Leon, I.R.; Rocha, S.L.; Oliveira-Carvalho, A.L.; et al. Bothrops insularis venomics: A proteomic analysis supported by transcriptomic-generated sequence data. J. Proteom. 2009, 72, 241–255. [CrossRef] [PubMed] Tashima, A.K.; Sanz, L.; Camargo, A.C.; Serrano, S.M.; Calvete, J.J. Snake venomics of the brazilian pitvipers Bothrops cotiara and Bothrops fonsecai. Identification of taxonomy markers. J. Proteom. 2008, 71, 473–485. [CrossRef] [PubMed] Alape-Giron, A.; Sanz, L.; Escolano, J.; Flores-Diaz, M.; Madrigal, M.; Sasa, M.; Calvete, J.J. Snake venomics of the lancehead pitviper Bothrops asper: Geographic, individual, and ontogenetic variations. J. Proteom. Res. 2008, 7, 3556–3571. [CrossRef] [PubMed] Angulo, Y.; Lomonte, B. Biochemistry and toxicology of toxins purified from the venom of the snake Bothrops asper. Toxicon 2009, 54, 949–957. [CrossRef] [PubMed] Lomonte, B.; Escolano, J.; Fernandez, J.; Sanz, L.; Angulo, Y.; Gutierrez, J.M.; Calvete, J.J. Snake venomics and antivenomics of the arboreal neotropical pitvipers Bothriechis lateralis and Bothriechis schlegelii. J. Proteom. Res. 2008, 7, 2445–2457. [CrossRef] [PubMed] Sanz, L.; Escolano, J.; Ferretti, M.; Biscoglio, M.J.; Rivera, E.; Crescenti, E.J.; Angulo, Y.; Lomonte, B.; Gutierrez, J.M.; Calvete, J.J. Snake venomics of the south and central american bushmasters. Comparison of the toxin composition of Lachesis muta gathered from proteomic versus transcriptomic analysis. J. Proteom. 2008, 71, 46–60. [CrossRef] [PubMed] Bogarin, G.; Romero, M.; Rojas, G.; Lutsch, C.; Casadamont, M.; Lang, J.; Otero, R.; Gutierrez, J.M. Neutralization, by a monospecific bothrops lanceolatus antivenom, of toxic activities induced by homologous and heterologous Bothirops snake venoms. Toxicon 1999, 37, 551–557. [CrossRef] Otero, R.; Gutierrez, J.; Beatriz Mesa, M.; Duque, E.; Rodriguez, O.; Luis Arango, J.; Gomez, F.; Toro, A.; Cano, F.; Maria Rodriguez, L.; et al. Complications of Bothrops, Porthidium, and Bothriechis snakebites in Colombia. A clinical and epidemiological study of 39 cases attended in a university hospital. Toxicon 2002, 40, 1107–1114. [CrossRef] Otero, R.; Leon, G.; Gutierrez, J.M.; Rojas, G.; Toro, M.F.; Barona, J.; Rodriguez, V.; Diaz, A.; Nunez, V.; Quintana, J.C.; et al. Efficacy and safety of two whole IgG polyvalent antivenoms, refined by caprylic acid fractionation with or without beta-propiolactone, in the treatment of Bothrops asper bites in Colombia. Trans. R. Soc. Trop. Med. Hyg. 2006, 100, 1173–1182. [CrossRef] [PubMed] Otero-Patino, R. Epidemiological, clinical and therapeutic aspects of Bothrops asper bites. Toxicon 2009, 54, 998–1011. [CrossRef] [PubMed] Calvete, J.J.; Perez, A.; Lomonte, B.; Sanchez, E.E.; Sanz, L. Snake venomics of crotalus tigris: The minimalist toxin arsenal of the deadliest nearctic rattlesnake venom. Evolutionary clues for generating a pan-specific antivenom against crotalid type ii venoms [corrected]. J. Proteom. Res. 2012, 11, 1382–1390. [CrossRef] [PubMed] Quintana, J.C.; Chacon, A.M.; Vargas, L.; Segura, C.; Gutierrez, J.M.; Alarcon, J.C. Antiplasmodial effect of the venom of Crotalus durissus cumanensis, crotoxin complex and crotoxin B. Acta Trop. 2012, 124, 126–132. [CrossRef] [PubMed] Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680–685. [CrossRef] [PubMed] BLAST Search. Available online: http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=Proteins (accessed on 12 May 2017). Mascot. Matrix Science Mascot Ms/Ms Ion Search. Available online: http://www.matrixscience.com/cgi/ search_form.pl?FORMVER=2&SEARCH=MIS (accessed on 12 May 2017). |
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Quintana Castillo, Juan CarlosVargas Muñoz, Leidy JohanaSegura Latorre, CésarEstrada Gómez, SebastiánBueno Sánchez, Julio CésarAlarcón, Juan Carlos102020-01-15T15:24:30Z2020-01-15T15:24:30Z2018-02-172072-665110.3390/toxins10020085https://hdl.handle.net/20.500.12494/15917Quintana Castillo, J.C., Vargas, L.J., Segura, C., Estrada Gómez, S., Bueno Sánchez, J.C. y Alarcón, J.C. (2018). Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study. Toxins 2018, 10, 85. Recuperado de:The Colombian rattlesnake Crotalus durissus cumanensis is distributed in three geographic zones of the country: the Atlantic Coast, the upper valley of the Magdalena River, and the eastern plains of the Colombian Orinoquía. Its venom induces neurological symptoms, such as eyelid ptosis, myasthenic facies, and paralysis of the respiratory muscles, which can lead to death. Identification and analysis of C. d. cumanensis showed nine groups of proteins responsible for the neurotoxic effect, of which the crotoxin complex was the most abundant (64.71%). Immunorecognition tests of C. d. cumanensis showed that the use of a commercial antivenom manufactured in Mexico resulted in immunoreactivity.https://scienti.colciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000580023juan.quintanac@campusucc.edu.coleidy.vargasmu@campusucc.edu.cocesar.segura@udea.edu.cosebastian.estrada@udea.edu.cojulio.bueno@udea.edu.cojuan.alarcon@udea.edu.co12Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Medellín y EnvigadoMedicinaMedellínhttps://www.mdpi.com/2072-6651/10/2/85ToxinsCampbell, J.A.; Lamar,W. The Venomous Reptiles of theWestern Hemisphere; Cornell University Press: New York, NY, USA, 2004; Volume II, p. 870.Klauber, L.M. Rattlesnakes: Their Habits, Life Histories, and Influence on Mankind; Zoological Society of San Diego: San Diego, CA, USA, 1972.Bucaretchi, F.; Herrera, S.R.; Hyslop, S.; Baracat, E.C.; Vieira, R.J. Snakebites by Crotalus durissus ssp. in children in Campinas, Sao Paulo, Brazil. Rev. Inst. Med. Trop. São Paulo 2002, 44, 133–138. [CrossRef] [PubMed]Oshima-Franco, Y.; Hyslop, S.; Prado-Franceschi, J.; Cruz-Hofling, M.A.; Rodrigues-Simioni, L. Neutralizing capacity of antisera raised in horses and rabbits against Crotalus durissus terrificus (South American rattlesnake) venom and its main toxin, crotoxin. Toxicon 1999, 37, 1341–1357. [CrossRef]Bon, C. Multicomponent neurotoxic phospholipases A2. In Venom Phospholipase A2 Enzymes: Structure, Function and Mechanism;Wiley: Chichester, UK, 1997; pp. 269–285.A2. 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Available online: http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=Proteins (accessed on 12 May 2017).Mascot. Matrix Science Mascot Ms/Ms Ion Search. Available online: http://www.matrixscience.com/cgi/ search_form.pl?FORMVER=2&SEARCH=MIS (accessed on 12 May 2017).C. d. cumanensis venomantivenomHPLC-nESI-MS/MSpeptide identificationCharacterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic StudyArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribución – No comercial – Sin Derivarinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationTEXT2018_Quintanaycolaboradores_Characterization_Venom_Cdcumanesis_Colombia.pdf.txt2018_Quintanaycolaboradores_Characterization_Venom_Cdcumanesis_Colombia.pdf.txtExtracted 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