Diagnóstico de cetosis subclínica y balance energético negativo en vacas lecheras mediante el uso de muestras de sangre, orina y leche

The objective of this study was to compare and associate plasma concentrations of ß-hidroxi-butyrate (ßHB) and subclinical ketosis with blood, urinary and milk indicators in dairy cow. Seventy four Holstein-Frisian cows were used from the third week pre-partum until the eighth week post-partum. Samples of blood, urine and milk were obtained weekly to determine the plasma concentrations of ßHB, non-esterified fatty acids (NEFA) and glucose. Rothera test reaction, excretion of ßHB and pH were determined in urine samples. The concentrations of fat, protein and urea were determined on milk samples. The for subclinical ketosis were considered as: ßHB > 1.0, > 1.2 and >1.4 mmol/L. Negative energetic balance (NEB) presentation was considered when NEFA >300 µmol/L during pre-partum and when ßHB > 0.6 mmol/L during post-partum. The intensity in the reaction of Rothera test and the excretion of urinary ßHB were related with plasmatic concentrations of ßHB (r=0.57, P<0.05 and r= 0.42, P<0.5, respectively). The Rothera test associated with ketosis at the three desition limits (P<0.05), BEN (P<0.05). The relations between plasma ßHB with NEFA and glucose were lower (P<0.05), and not significant (P>0.05) with milk fat or protein content or urinary pH. In conclusion, slight and moderate reactions of the Rothera test are good indicators of NEB, while an intense test result indicates subclinical ketosis.

• Autores: Cucunubo L.G.; Strieder Barboza, Clarissa; Wittwer F.; Noro M.
• Tipo de recurso: Article of journal
• Fecha de publicación: 2013
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/42757
• Acceso en línea: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84877803680&partnerID=40&md5=1d5b9c6ac1d15bbaf8651803ebe70bd4; https://hdl.handle.net/20.500.12494/42757
• Palabra clave: ßHB; Glucose; Ketosis; Negative energetic balance; Rothera test
• Rights: closedAccess
• License: http://purl.org/coar/access_right/c_14cb