Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis

Renal involvement in Systemic Lupus Erythematous (SLE) patients is one of the leading causes of morbidity and a significant contributor to mortality. It’s estimated that nearly 50% of SLE individuals develop kidney disease in the first year of the diagnosis. Class IV lupus nephritis (LN-IV) is the class of lupus nephritis most common in Colombian patients with SLE. Altered miRNAs expression levels have been reported in human autoimmune diseases including lupus. Variations in the expression pattern of peripheral blood circulating miRNAs specific for this class of lupus nephritis could be correlated with the pathophysiological status of this group of individuals. The aim of this study was to evaluate the relative abundance of circulating microRNAs in peripheral blood from Colombian patients with LN-IV. Circulating miRNAs in plasma of patients with diagnosis of LN-IV were compared with individuals without renal involvement (LNN group) and healthy individuals (CTL group). Total RNA was extracted from 10 ml of venous blood and subsequently sequenced using Illumina. The sequences were processed and these were analyzed using miRBase and Ensembl databases. Differential gene expression analysis was carried out with edgeR and functional analysis were done with DIANA-miRPath. Analysis was carried out using as variables of selection fold change (2 o -2) and false discovery rate (0.05). We identified 24 circulating microRNAs with differential abundance between LN-IV and CTL groups, fourteen of these microRNAs are described for the first time to lupus nephritis (hsa-miR-589-3p, hsa-miR-1260b, hsa-miR-4511, hsa-miR-485-5p, hsa-miR-584-5p, hsa-miR-543, hsa-miR-153-3p, hsa-miR-6087, hsa-miR-3942-5p, hsa-miR-7977, hsa-miR-323b-3p, hsa-miR-4732-3p and hsa-miR-6741-3p). These changes in the abundance of miRNAs could be interpreted as alterations in the miRNAs-mRNA regulatory network in the pathogenesis of LN, preceding the clinical onset of the disease. The findings thus contribute to understanding the disease process and are likely to pave the way towards identifying disease biomarkers for early diagnosis of LN. © 2017 Navarro-Quiroz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

• Autores: Navarro-Quiroz E.; Pacheco-Lugo L.; Navarro Quiroz, Roberto Carlos; Lorenzi H.; España-Puccini P.; Díaz-Olmos Y.; Almendrales L.; Olave V.; Gonzalez-Torres H.; Diaz-Perez A.; Dominguez A.; Iglesias A.; García R.; Aroca-Martinez G.
• Tipo de recurso: Article of journal
• Fecha de publicación: 2017
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/41911
• Acceso en línea: https://doi.org/10.17533/udea.rfo.v28n2a5; https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021626638&doi=10.3303%2fCET1757296&partnerID=40&md5=c05c5ffed767411ff5ef0dbedf8921bb; https://hdl.handle.net/20.500.12494/41911
• Palabra clave: microRNA; microRNA 1260b; microRNA 153 3p; microRNA 323b 3p; microRNA 3942 5p; microRNA 4511; microRNA 4732 3p; microRNA 485 5p; microRNA 543; microRNA 584 5p; microRNA 589 3p; microRNA 6087; microRNA 6741 3p; microRNA 7977; unclassified drug; adolescent; adult; aged; Article; blood sampling; clinical article; cohort analysis; Colombian; comparative study; controlled study; data base; disease classification; DNA fingerprinting; female; gene control; gene expression; gene identification; gene mutation; human; lupus erythematosus nephritis; male; onset age; pathogenesis; RNA analysis; RNA extraction; RNA sequence
• Rights: closedAccess
• License: http://purl.org/coar/access_right/c_14cb