Prevalence and molecular typing of rotavirus in children with acute diarrhoea in Northeastern Colombia.

After the introduction of the rotavirus vaccine, the number of rotavirus-associated deaths and the predicted annual rotavirus detection rate had slightly declined worldwide. Taking in account that in Colombia, Rotarix vaccine was introduced in 2009, the purpose of this study was to evaluate the pres...

Full description

Autores:
Martínez Gutiérrez, Marlén
Arcila Quiceno, Victor
Trejos-Suarez J
Ruiz Saenz, Julian
Tipo de recurso:
Article of journal
Fecha de publicación:
2023
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/50900
Acceso en línea:
https://doi.org/10.1590/S1678-9946201961034
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069267283&doi=10.1590%2fs1678-9946201961034&partnerID=40&md5=a563f1492b962917eec683e3cbc5324f
https://hdl.handle.net/20.500.12494/50900
Palabra clave:
ACUTE DIARRHEA
ACUTE DISEASE
ACUTE GASTROENTERITIS
AGE DISTRIBUTION
ARTICLE
CLASSIFICATION
COLOMBIA
COMPLEMENTARY DNA
CROSS-SECTIONAL STUDIES
CROSS-SECTIONAL STUDY
CHILD
CHILD, PRESCHOOL
DEHYDRATION
DIARRHEA
DNA SYNTHESIS
ENZYME LINKED IMMUNOSORBENT ASSAY
ENZYME-LINKED IMMUNOSORBENT ASSAY
FECES
FECES ANALYSIS
FEMALE
GENETICS
GENOTYPE
GENOTYPING TECHNIQUE
HOSPITALIZATION
HUMAN
HUMANS
IMMUNOAFFINITY CHROMATOGRAPHY
IMMUNOLOGICAL PROCEDURES
INFANT
INFANT, NEWBORN
MALE
MIXED INFECTION
MOLECULAR TYPING
NEWBORN
PHYLOGENY
POLYMERASE CHAIN REACTION
PRESCHOOL CHILD
PREVALENCE
REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION
RNA EXTRACTION
RNA, VIRAL
ROTAVIRUS
ROTAVIRUS A
ROTAVIRUS INFECTION
ROTAVIRUS INFECTIONS
ROTAVIRUS VACCINE
SPECTROPHOTOMETRY
VACCINATION
VIROLOGY
VIRUS RNA
VOMITING
Rights
openAccess
License
http://purl.org/coar/access_right/c_abf2
Description
Summary:After the introduction of the rotavirus vaccine, the number of rotavirus-associated deaths and the predicted annual rotavirus detection rate had slightly declined worldwide. Taking in account that in Colombia, Rotarix vaccine was introduced in 2009, the purpose of this study was to evaluate the presence of rotavirus A in children under five years who were treated for acute diarrhoea in Bucaramanga, Colombia and, moreover, to determine the genotypes of rotavirus present in those children. We performed an analytical cross-sectional study of rotavirus A in faecal samples from children up to five years of age. Stool samples were screened for rotavirus A using a lateral-flow immunochromatographic assay and confirmed using a VP6 sandwich ELISA. Genotyping of rotavirus A-positive samples was performed by PCR and sequencing of VP7 and VP4 genes. The overall prevalence of rotavirus was 30.53% (95% confidence interval [CI] 21.2 - 39.7). Most of the children with rotavirus (86.2%) had received two doses of the rotavirus vaccine. G3 strains accounted for the vast majority of cases (82.8%), followed by G12 strains (13.8%) and G3/G9 coinfections (3.4%). Among the P genotypes, P[8] was the most prevalent (69%), followed by P[9] (31%). The most common G[P] genotype combination was G3P[8], followed by G3P[9]. The main finding in this study was that rotavirus, in a Colombian region, is still an important pathogen in children under five years old, previously vaccinated. The results showed that different factors, such as kindergarten attendance, could explain the epidemiology and transmission of rotavirus in Bucaramanga.