Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels

Pamphobeteus verdolaga is a recently described Theraphosidae spider from the Andean region of Colombia. Previous reports partially characterized its venom profile. In this study, we conducted a detailed analysis that includes reversed-phase high-performance liquid chromatography (rp-HPLC), calcium i...

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Autores:
Estrada Gomez, Sebastian
Caldas Cardoso, Fernanda
Vargas Muñoz, Leidy Johana
Quintana Castillo, Juan Carlos
Arenas Gómez, Claudia Marcela
Pineda, Sandy Steffany
Saldarriaga Cordoba, Monica Maria
Tipo de recurso:
Article of journal
Fecha de publicación:
2019
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/41485
Acceso en línea:
https://doi.org/10.1016/j.rcp.2014.02.004
https://hdl.handle.net/20.500.12494/41485
Palabra clave:
Pamphobeteus
disulfide-rich peptide (DRP)
inhibitory cysteine knot (ICK)
ion channels
peptides
theraphosidae
transcriptome
venomics
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closedAccess
License
http://purl.org/coar/access_right/c_14cb
id COOPER2_608721035accefeca287ca36bf385108
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/41485
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
spelling Estrada Gomez, SebastianCaldas Cardoso, FernandaVargas Muñoz, Leidy JohanaQuintana Castillo, Juan CarlosArenas Gómez, Claudia MarcelaPineda, Sandy SteffanySaldarriaga Cordoba, Monica Maria2021-12-16T22:15:33Z2021-12-16T22:15:33Z2019https://doi.org/10.1016/j.rcp.2014.02.00420726651https://hdl.handle.net/20.500.12494/41485Estrada S,Caldas F,VARGAS L,QUINTANA J,Arenas CM,Pineda SS,Saldarriaga MM. Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels. Toxins (Basel). 2019. 11. (496):p. 1-21. .Pamphobeteus verdolaga is a recently described Theraphosidae spider from the Andean region of Colombia. Previous reports partially characterized its venom profile. In this study, we conducted a detailed analysis that includes reversed-phase high-performance liquid chromatography (rp-HPLC), calcium influx assays, tandem mass spectrometry analysis (tMS/MS), and venom-gland transcriptome. rp-HPLC fractions of P. verdolaga venom showed activity on Ca V 2.2, Ca V 3.2, and Na V 1.7 ion channels. Active fractions contained several peptides with molecular masses ranging from 3399.4 to 3839.6 Da. The tMS/MS analysis of active fraction displaying the strongest activity to inhibit calcium channels showed sequence fragments similar to one of the translated transcripts detected in the venom-gland transcriptome. The putative peptide of this translated transcript corresponded to a toxin, here named ?-theraphositoxin-Pv3a, a potential ion channel modulator toxin that is, in addition, very similar to other theraphositoxins affecting calcium channels (i.e., ?-theraphotoxin-Asp1a). Additionally, using this holistic approach, we found that P. verdolaga venom is an important source of disulfide-rich proteins expressing at least eight superfamilies.leidy.vargasmu@campusucc.edu.co21-1MDPI AGPamphobeteusdisulfide-rich peptide (DRP)inhibitory cysteine knot (ICK)ion channelspeptidestheraphosidaetranscriptomevenomicsVenomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium ChannelsArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionToxinsinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/41485oai:repository.ucc.edu.co:20.500.12494/414852024-08-20 16:21:44.122metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
title Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
spellingShingle Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
Pamphobeteus
disulfide-rich peptide (DRP)
inhibitory cysteine knot (ICK)
ion channels
peptides
theraphosidae
transcriptome
venomics
title_short Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
title_full Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
title_fullStr Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
title_full_unstemmed Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
title_sort Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
dc.creator.fl_str_mv Estrada Gomez, Sebastian
Caldas Cardoso, Fernanda
Vargas Muñoz, Leidy Johana
Quintana Castillo, Juan Carlos
Arenas Gómez, Claudia Marcela
Pineda, Sandy Steffany
Saldarriaga Cordoba, Monica Maria
dc.contributor.author.none.fl_str_mv Estrada Gomez, Sebastian
Caldas Cardoso, Fernanda
Vargas Muñoz, Leidy Johana
Quintana Castillo, Juan Carlos
Arenas Gómez, Claudia Marcela
Pineda, Sandy Steffany
Saldarriaga Cordoba, Monica Maria
dc.subject.spa.fl_str_mv Pamphobeteus
disulfide-rich peptide (DRP)
inhibitory cysteine knot (ICK)
ion channels
peptides
theraphosidae
transcriptome
venomics
topic Pamphobeteus
disulfide-rich peptide (DRP)
inhibitory cysteine knot (ICK)
ion channels
peptides
theraphosidae
transcriptome
venomics
description Pamphobeteus verdolaga is a recently described Theraphosidae spider from the Andean region of Colombia. Previous reports partially characterized its venom profile. In this study, we conducted a detailed analysis that includes reversed-phase high-performance liquid chromatography (rp-HPLC), calcium influx assays, tandem mass spectrometry analysis (tMS/MS), and venom-gland transcriptome. rp-HPLC fractions of P. verdolaga venom showed activity on Ca V 2.2, Ca V 3.2, and Na V 1.7 ion channels. Active fractions contained several peptides with molecular masses ranging from 3399.4 to 3839.6 Da. The tMS/MS analysis of active fraction displaying the strongest activity to inhibit calcium channels showed sequence fragments similar to one of the translated transcripts detected in the venom-gland transcriptome. The putative peptide of this translated transcript corresponded to a toxin, here named ?-theraphositoxin-Pv3a, a potential ion channel modulator toxin that is, in addition, very similar to other theraphositoxins affecting calcium channels (i.e., ?-theraphotoxin-Asp1a). Additionally, using this holistic approach, we found that P. verdolaga venom is an important source of disulfide-rich proteins expressing at least eight superfamilies.
publishDate 2019
dc.date.issued.none.fl_str_mv 2019
dc.date.accessioned.none.fl_str_mv 2021-12-16T22:15:33Z
dc.date.available.none.fl_str_mv 2021-12-16T22:15:33Z
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.rcp.2014.02.004
dc.identifier.issn.spa.fl_str_mv 20726651
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/41485
dc.identifier.bibliographicCitation.spa.fl_str_mv Estrada S,Caldas F,VARGAS L,QUINTANA J,Arenas CM,Pineda SS,Saldarriaga MM. Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels. Toxins (Basel). 2019. 11. (496):p. 1-21. .
url https://doi.org/10.1016/j.rcp.2014.02.004
https://hdl.handle.net/20.500.12494/41485
identifier_str_mv 20726651
Estrada S,Caldas F,VARGAS L,QUINTANA J,Arenas CM,Pineda SS,Saldarriaga MM. Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels. Toxins (Basel). 2019. 11. (496):p. 1-21. .
dc.relation.ispartofjournal.spa.fl_str_mv Toxins
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/closedAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_14cb
eu_rights_str_mv closedAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_14cb
dc.format.extent.spa.fl_str_mv 21-1
dc.publisher.spa.fl_str_mv MDPI AG
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
_version_ 1808789293499416576

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