NK Cell Activity and CD57+/NKG2Chigh Phenotype Are Increased in Men Who Have Sex With Men at High Risk for HIV

Introduction: The HIV-exposed seronegative (HESN) status is for individuals who remain seronegative despite repeated exposure to HIV. One of the main cohorts within this group is men who have sex with men (MSM). Studies of this cohort have revealed different immunological and genetic mechanisms that...

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Autores:: Zapata Builes, Wildeman
Flórez Álvarez, Lizdany
Blanquiceth, Yurany
Ramírez, Katherin
Ossa Giraldo, Ana Claudia
Velilla Hernandez, Paula Andrea
Hernández López, Juan Carlos

Tipo de recurso:: Article of journal

Fecha de publicación:: 2020

Institución:: Universidad Cooperativa de Colombia

Repositorio:: Repositorio UCC

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repository_id_str
dc.title.spa.fl_str_mv	NK Cell Activity and CD57+/NKG2Chigh Phenotype Are Increased in Men Who Have Sex With Men at High Risk for HIV
title	NK Cell Activity and CD57+/NKG2Chigh Phenotype Are Increased in Men Who Have Sex With Men at High Risk for HIV
spellingShingle	NK Cell Activity and CD57+/NKG2Chigh Phenotype Are Increased in Men Who Have Sex With Men at High Risk for HIV CD57C/NKG2Chigh NK cell cytotoxicity Highly exposed seronegative men who have sex with men natural resistance HIV CD57C/NKG2Chigh NK cell cytotoxicity Highly exposed seronegative men who have sex with men natural resistance HIV
title_short	NK Cell Activity and CD57+/NKG2Chigh Phenotype Are Increased in Men Who Have Sex With Men at High Risk for HIV
title_full	NK Cell Activity and CD57+/NKG2Chigh Phenotype Are Increased in Men Who Have Sex With Men at High Risk for HIV
title_fullStr	NK Cell Activity and CD57+/NKG2Chigh Phenotype Are Increased in Men Who Have Sex With Men at High Risk for HIV
title_full_unstemmed	NK Cell Activity and CD57+/NKG2Chigh Phenotype Are Increased in Men Who Have Sex With Men at High Risk for HIV
title_sort	NK Cell Activity and CD57+/NKG2Chigh Phenotype Are Increased in Men Who Have Sex With Men at High Risk for HIV
dc.creator.fl_str_mv	Zapata Builes, Wildeman Flórez Álvarez, Lizdany Blanquiceth, Yurany Ramírez, Katherin Ossa Giraldo, Ana Claudia Velilla Hernandez, Paula Andrea Hernández López, Juan Carlos
dc.contributor.author.none.fl_str_mv	Zapata Builes, Wildeman Flórez Álvarez, Lizdany Blanquiceth, Yurany Ramírez, Katherin Ossa Giraldo, Ana Claudia Velilla Hernandez, Paula Andrea Hernández López, Juan Carlos
dc.subject.spa.fl_str_mv	CD57C/NKG2Chigh NK cell cytotoxicity Highly exposed seronegative men who have sex with men natural resistance HIV
topic	CD57C/NKG2Chigh NK cell cytotoxicity Highly exposed seronegative men who have sex with men natural resistance HIV CD57C/NKG2Chigh NK cell cytotoxicity Highly exposed seronegative men who have sex with men natural resistance HIV
dc.subject.other.spa.fl_str_mv	CD57C/NKG2Chigh NK cell cytotoxicity Highly exposed seronegative men who have sex with men natural resistance HIV
description	Introduction: The HIV-exposed seronegative (HESN) status is for individuals who remain seronegative despite repeated exposure to HIV. One of the main cohorts within this group is men who have sex with men (MSM). Studies of this cohort have revealed different immunological and genetic mechanisms that can explain the phenomenon of natural HIV resistance. NK cells’ higher effector capacity is related to natural resistance to HIV. Besides, a new population of NK cells with adaptive features was described recently. These cells are increased in some HESN cohorts and appear to be involved in better control of viral replication in primarily HIV-infected subjects. The present study evaluated the role of NK cells in the natural resistance to HIV-1 infection in MSM. Methodology: Phenotypic and functional features were evaluated in NK cells from two groups of MSM, at different risks of HIV infection, according to the number of sexual partners. The production of IFN-g and b-chemokines was included in the analysis, as well as the cytotoxic capacity and adaptive NK cell frequency. Genetic features, such as HLA and KIR allele frequencies, were also explored. Results: High-risk MSM exhibit an increased frequency of fully mature and CD57C/NKG2Chigh NK cells. These individuals also show higher cytotoxic capacity and IFN-g production in response to K562 stimuli. NK cells with a CD107aC/IFN-gC functional profile were found more frequently and displayed higher IFN-g production capacity among high-risk MSM than among low-risk MSM. The protective allele HLAB 18 was only present in the high-risk MSM group as well as HLA-B 39. The protective phenotype KIR3DL1/S1-HLA-B Bw4, in a homozygous state, was particularly abundant in the high-risk population. Notably, some of these functional features were related to higher frequencies of mature and CD57C/NKG2Chigh NK cells, which, in turn, were associated with a higher number of sexual partners. Conclusion: The changes observed in the NK cell compartment can be driven by the magnitude of sexual exposure and immunological challenges of high-risk individuals, which could influence their resistance/susceptibility to HIV infection.
publishDate	2020
dc.date.accessioned.none.fl_str_mv	2020-11-27T16:29:52Z
dc.date.available.none.fl_str_mv	2020-11-27T16:29:52Z
dc.date.issued.none.fl_str_mv	2020-09-11
dc.type.none.fl_str_mv	Artículo
dc.type.coar.fl_str_mv	http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv	http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv	info:eu-repo/semantics/article
dc.type.version.none.fl_str_mv	info:eu-repo/semantics/publishedVersion
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dc.identifier.issn.spa.fl_str_mv	1664-3224
dc.identifier.uri.spa.fl_str_mv	10.3389/fimmu.2020.537044
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/20.500.12494/28302
dc.identifier.bibliographicCitation.spa.fl_str_mv	Flórez-Álvarez, L., Blanquiceth, Y., Ramírez, K., Ossa-Giraldo, A. C., Velilla, P. A., Hernandez, J. C. y Zapata, W. (2020) NK Cell Activity and CD57+/NKG2Chigh Phenotype Are Increased in Men Who Have Sex With Men at High Risk for HIV
identifier_str_mv	1664-3224 10.3389/fimmu.2020.537044 Flórez-Álvarez, L., Blanquiceth, Y., Ramírez, K., Ossa-Giraldo, A. C., Velilla, P. A., Hernandez, J. C. y Zapata, W. (2020) NK Cell Activity and CD57+/NKG2Chigh Phenotype Are Increased in Men Who Have Sex With Men at High Risk for HIV
url	https://hdl.handle.net/20.500.12494/28302
dc.relation.isversionof.spa.fl_str_mv	https://www.frontiersin.org/articles/10.3389/fimmu.2020.537044/full?report=reader
dc.relation.ispartofjournal.spa.fl_str_mv	Frontiers in Immunology
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Expression patterns of NKG2A, KIR, and CD57 deﬁne a process of CD56dim NK-cell diﬀerentiation uncoupled from NK-cell education. Blood. (2010) 116:3853–64. doi: 10.1182/blood-2010-04-281675 Le Garﬀ-Tavernier M, Beziat V, Decocq J, Siguret V, Gandjbakhch F, Pautas E, et al. Human NK cells display major phenotypic and functional changes over the life span. Aging Cell. (2010) 9:527–35. doi: 10.1111/j.1474-9726.2010. 00584.x Goodier MR, White MJ, Darboe A, Nielsen CM, Goncalves A, Bottomley C, et al. Rapid NK cell diﬀerentiation in a population with near-universal human cytomegalovirus infection is attenuated by NKG2C deletions. Blood. (2014) 124:2213–22. doi: 10.1182/blood-2014-05-576124 Foley B, Cooley S, Verneris MR, Pitt M, Curtsinger J, Luo X, et al. Cytomegalovirus reactivation after allogeneic transplantation promotes a lasting increase in educated NKG2C+ natural killer cells with potent function. Blood. (2012) 119:2665–74. doi: 10.1182/blood-2011-10-386995 Bjorkstrom NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, et al. Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus. J Exp Med. (2011) 208:13–21. doi: 10.1084/ jem.20100762 Beziat V, Dalgard O, Asselah T, Halfon P, Bedossa P, Boudifa A, et al. CMV drives clonal expansion of NKG2C+ NK cells expressing self-speciﬁc KIRs in chronic hepatitis patients. Eur J Immunol. (2012) 42:447–57. doi: 10.1002/eji. 201141826 Petitdemange C, Becquart P, Wauquier N, Beziat V, Debre P, Leroy EM, et al. Unconventional repertoire proﬁle is imprinted during acute chikungunya infection for natural killer cells polarization toward cytotoxicity. PLoS Pathog. (2011) 7:e1002268. doi: 10.1371/journal.ppat.1002268 Lopez-Vergès S, Milush JM, Pandey S, York VA, Arakawa-Hoyt J, Pircher H, et al. CD57 deﬁnes a functionally distinct population of mature NK cells in the human CD56dimCD16+ NK-cell subset. 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Human leukocyte antigen (HLA) B∗18 and protection against mother-to-child HIV type 1 transmission. AIDS Res Hum Retroviruses. (2004) 20:692–7. doi: 10.1089/0889222041524616 Chaudhari DV, Chavan VR, Ahir SP, Kerkar SC, Mehta PR, Mania- Pramanik J. Human leukocyte antigen B distribution in HIV discordant cohort from India. Immunol Lett. (2013) 156:1–6. doi: 10.1016/j.imlet.2013. 09.002 Valenzuela-Ponce H, Alva-Hernandez S, Garrido-Rodriguez D, Soto-Nava M, Garcia-Tellez T, Escamilla-Gomez T, et al. Novel HLA class I associations with HIV-1 control in a unique genetically admixed population. Sci Rep. (2018) 8:6111. Payne R, Muenchhoﬀ M, Mann J, Roberts HE, Matthews P, Adland E, et al. Impact of HLA-driven HIV adaptation on virulence in populations of high HIV seroprevalence. Proc Natl Acad Sci USA. (2014) 111:E5393–400. Raulet DH. Missing self recognition and self tolerance of natural killer (NK) cells. Semin Immunol. (2006) 18:145–50. doi: 10.1016/j.smim.2006.03.003 Alter G, Rihn S, Walter K, Nolting A, Martin M, Rosenberg ES, et al. HLA class I subtype-dependent expansion of KIR3DS1+ and KIR3DL1+ NK cells during acute human immunodeﬁciency virus type 1 infection. J Virol. (2009) 83:6798–805. doi: 10.1128/jvi.00256-09 Boudreau JE, Mulrooney TJ, Le Luduec JB, Barker E, Hsu KC. KIR3DL1 and HLA-B density and binding calibrate NK education and response to HIV. J Immunol. (2016) 196:3398–410. doi: 10.4049/jimmunol.1502469 Boulet S, Song R, Kamya P, Bruneau J, Shoukry NH, Tsoukas CM, et al. HIV protective KIR3DL1 and HLA-B genotypes inﬂuence NK cell function following stimulation with HLA-devoid cells. J Immunol. (2010) 184:2057–64. doi: 10.4049/jimmunol.0902621 Song R, Lisovsky I, Lebouche B, Routy JP, Bruneau J, Bernard NF. HIV protective KIR3DL1/S1-HLA-B genotypes inﬂuence NK cell-mediated inhibition of HIV replication in autologous CD4 targets. PLoS Pathog. 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spelling	Zapata Builes, WildemanFlórez Álvarez, LizdanyBlanquiceth, YuranyRamírez, KatherinOssa Giraldo, Ana ClaudiaVelilla Hernandez, Paula AndreaHernández López, Juan Carlos 11:5370442020-11-27T16:29:52Z2020-11-27T16:29:52Z2020-09-111664-322410.3389/fimmu.2020.537044https://hdl.handle.net/20.500.12494/28302Flórez-Álvarez, L., Blanquiceth, Y., Ramírez, K., Ossa-Giraldo, A. C., Velilla, P. A., Hernandez, J. C. y Zapata, W. (2020) NK Cell Activity and CD57+/NKG2Chigh Phenotype Are Increased in Men Who Have Sex With Men at High Risk for HIVIntroduction: The HIV-exposed seronegative (HESN) status is for individuals who remain seronegative despite repeated exposure to HIV. One of the main cohorts within this group is men who have sex with men (MSM). Studies of this cohort have revealed different immunological and genetic mechanisms that can explain the phenomenon of natural HIV resistance. NK cells’ higher effector capacity is related to natural resistance to HIV. Besides, a new population of NK cells with adaptive features was described recently. These cells are increased in some HESN cohorts and appear to be involved in better control of viral replication in primarily HIV-infected subjects. The present study evaluated the role of NK cells in the natural resistance to HIV-1 infection in MSM. Methodology: Phenotypic and functional features were evaluated in NK cells from two groups of MSM, at different risks of HIV infection, according to the number of sexual partners. The production of IFN-g and b-chemokines was included in the analysis, as well as the cytotoxic capacity and adaptive NK cell frequency. Genetic features, such as HLA and KIR allele frequencies, were also explored. Results: High-risk MSM exhibit an increased frequency of fully mature and CD57C/NKG2Chigh NK cells. These individuals also show higher cytotoxic capacity and IFN-g production in response to K562 stimuli. NK cells with a CD107aC/IFN-gC functional profile were found more frequently and displayed higher IFN-g production capacity among high-risk MSM than among low-risk MSM. The protective allele HLAB 18 was only present in the high-risk MSM group as well as HLA-B 39. The protective phenotype KIR3DL1/S1-HLA-B Bw4, in a homozygous state, was particularly abundant in the high-risk population. Notably, some of these functional features were related to higher frequencies of mature and CD57C/NKG2Chigh NK cells, which, in turn, were associated with a higher number of sexual partners. Conclusion: The changes observed in the NK cell compartment can be driven by the magnitude of sexual exposure and immunological challenges of high-risk individuals, which could influence their resistance/susceptibility to HIV infection.Introduction: The HIV-exposed seronegative (HESN) status is for individuals who remain seronegative despite repeated exposure to HIV. One of the main cohorts within this group is men who have sex with men (MSM). Studies of this cohort have revealed different immunological and genetic mechanisms that can explain the phenomenon of natural HIV resistance. NK cells’ higher effector capacity is related to natural resistance to HIV. Besides, a new population of NK cells with adaptive features was described recently. These cells are increased in some HESN cohorts and appear to be involved in better control of viral replication in primarily HIV-infected subjects. The present study evaluated the role of NK cells in the natural resistance to HIV-1 infection in MSM. Methodology: Phenotypic and functional features were evaluated in NK cells from two groups of MSM, at different risks of HIV infection, according to the number of sexual partners. The production of IFN-g and b-chemokines was included in the analysis, as well as the cytotoxic capacity and adaptive NK cell frequency. Genetic features, such as HLA and KIR allele frequencies, were also explored. Results: High-risk MSM exhibit an increased frequency of fully mature and CD57C/NKG2Chigh NK cells. These individuals also show higher cytotoxic capacity and IFN-g production in response to K562 stimuli. NK cells with a CD107aC/IFN-gC functional profile were found more frequently and displayed higher IFN-g production capacity among high-risk MSM than among low-risk MSM. The protective allele HLAB 18 was only present in the high-risk MSM group as well as HLA-B 39. The protective phenotype KIR3DL1/S1-HLA-B Bw4, in a homozygous state, was particularly abundant in the high-risk population. Notably, some of these functional features were related to higher frequencies of mature and CD57C/NKG2Chigh NK cells, which, in turn, were associated with a higher number of sexual partners. Conclusion: The changes observed in the NK cell compartment can be driven by the magnitude of sexual exposure and immunological challenges of high-risk individuals, which could influence their resistance/susceptibility to HIV infection.https://scienti.minciencias.gov.co/cvlac/EnProdArticulo/query.do?cod_producto=73&cod_rh=0000157775https://orcid.org/0000-0002-7351-8738COL0112548wildeman.zapatab@campusucc.edu.cohttps://scholar.google.com.co/citations?hl=en&user=VLZxl1UAAAAJ14Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Medellín y EnvigadoChristian Körner, Heinrich-Pette-Institut, Leibniz-Institut für Experimentelle Virologie, GermanyMedicinaMedellínhttps://www.frontiersin.org/articles/10.3389/fimmu.2020.537044/full?report=readerFrontiers in ImmunologyHorton RE, McLaren PJ, Fowke K, Kimani J, Ball TB. Cohorts for the study of HIV−1–exposed but uninfected individuals: beneﬁts and limitations. J Infect Dis. 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(2001) 98:5140–5. doi: 10.1073/pnas.071548198CD57C/NKG2Chigh NK cellcytotoxicityHighly exposed seronegativemen who have sex with mennatural resistanceHIVCD57C/NKG2Chigh NK cellcytotoxicityHighly exposed seronegativemen who have sex with mennatural resistanceHIVNK Cell Activity and CD57+/NKG2Chigh Phenotype Are Increased in Men Who Have Sex With Men at High Risk for HIVArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribucióninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationLICENSElicense.txtlicense.txttext/plain; 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NK Cell Activity and CD57+/NKG2Chigh Phenotype Are Increased in Men Who Have Sex With Men at High Risk for HIV

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