Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method
Background: The brilliant cresyl blue (BCB) staining is a non-invasive test to select the best-suited oocytes for embryonic development. This makes it a useful tool to select best-quality oocytes at the times of the year when there is forage restriction. Objective: To evaluate the effect of seasonal...
- Autores:
-
Maia R.C.
Mogollon Waltero, Edgar mauricio
Dubeibe Marin, Diego fernando
Carvalho B.P.
Dias Á.J.B.
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2023
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/49621
- Acceso en línea:
- https://doi.org/10.17533/udea.rccp.v30n4a01
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031127791&doi=10.17533%2fudea.rccp.v30n4a01&partnerID=40&md5=06b7fd472cb906da1a723217d9762a22
https://hdl.handle.net/20.500.12494/49621
- Palabra clave:
- COLORIMETRIC TEST
CUMULUS-OOPHORUS COMPLEXES
IN VITRO MATURATION
PASTURE
SEASONS
- Rights
- openAccess
- License
- http://purl.org/coar/access_right/c_abf2
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Maia R.C.Mogollon Waltero, Edgar mauricioDubeibe Marin, Diego fernandoCarvalho B.P.Dias Á.J.B.2023-05-24T16:21:34Z2023-05-24T16:21:34Z01/10/2017https://doi.org/10.17533/udea.rccp.v30n4a01https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031127791&doi=10.17533%2fudea.rccp.v30n4a01&partnerID=40&md5=06b7fd472cb906da1a723217d9762a2201200690https://hdl.handle.net/20.500.12494/49621Maia R.C.,Mogollon Waltero Edgar mauricio,Dubeibe Marin Diego fernando,Carvalho B.P.,Dias Á.J.B..Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method.Revista Colombiana De Ciencias Pecuarias. 2017. 30. (4):p. 259-266Background: The brilliant cresyl blue (BCB) staining is a non-invasive test to select the best-suited oocytes for embryonic development. This makes it a useful tool to select best-quality oocytes at the times of the year when there is forage restriction. Objective: To evaluate the effect of seasonality on the nuclear maturation and quality of oocytes selected by the BCB test. Methods: The cumulus-oophorus complexes (COCs) were obtained in summer and winter of 2010 and 2011. Selected COCs were maintained for 90 min at 38.5 °C in a CO2 incubator, in TCM 199 medium containing 10% fetal bovine serum and antibiotics, and supplemented with 26 µM brilliant cresyl blue. Afterwards, they were divided according to the ooplasm staining (BCB+ —blue; BCB- —unstained). Subsequently, COCs were matured for 22 h. Nuclear maturation was evaluated at 22 h of culture. Results: The proportion of BCB- oocytes was higher in the winter of 2010, but there was no increase in this group in the winter of 2011. The percentage of oocytes that reached metaphase II was higher in control and BCB+ groups in relation to oocytes from BCB- group. Conclusion: The season of the year influences the percentage of oocytes best suited for embryonic production in situations in which oocyte donors receive pasture-based feeding, since the method was effective in determining the effect of seasonality on the competence of bovine oocytes to reach nuclear maturation. © 2017, Universidad de Antioquia. All rights reserved.edgar.mogollonw@campusucc.edu.co259-266Universidad de AntioquiaCOLORIMETRIC TESTCUMULUS-OOPHORUS COMPLEXESIN VITRO MATURATIONPASTURESEASONSEffect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue methodArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionRevista Colombiana De Ciencias Pecuariasinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Publication20.500.12494/49621oai:repository.ucc.edu.co:20.500.12494/496212024-08-20 16:22:23.778metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com |
dc.title.spa.fl_str_mv |
Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method |
title |
Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method |
spellingShingle |
Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method COLORIMETRIC TEST CUMULUS-OOPHORUS COMPLEXES IN VITRO MATURATION PASTURE SEASONS |
title_short |
Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method |
title_full |
Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method |
title_fullStr |
Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method |
title_full_unstemmed |
Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method |
title_sort |
Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method |
dc.creator.fl_str_mv |
Maia R.C. Mogollon Waltero, Edgar mauricio Dubeibe Marin, Diego fernando Carvalho B.P. Dias Á.J.B. |
dc.contributor.author.none.fl_str_mv |
Maia R.C. Mogollon Waltero, Edgar mauricio Dubeibe Marin, Diego fernando Carvalho B.P. Dias Á.J.B. |
dc.subject.spa.fl_str_mv |
COLORIMETRIC TEST CUMULUS-OOPHORUS COMPLEXES IN VITRO MATURATION PASTURE SEASONS |
topic |
COLORIMETRIC TEST CUMULUS-OOPHORUS COMPLEXES IN VITRO MATURATION PASTURE SEASONS |
description |
Background: The brilliant cresyl blue (BCB) staining is a non-invasive test to select the best-suited oocytes for embryonic development. This makes it a useful tool to select best-quality oocytes at the times of the year when there is forage restriction. Objective: To evaluate the effect of seasonality on the nuclear maturation and quality of oocytes selected by the BCB test. Methods: The cumulus-oophorus complexes (COCs) were obtained in summer and winter of 2010 and 2011. Selected COCs were maintained for 90 min at 38.5 °C in a CO2 incubator, in TCM 199 medium containing 10% fetal bovine serum and antibiotics, and supplemented with 26 µM brilliant cresyl blue. Afterwards, they were divided according to the ooplasm staining (BCB+ —blue; BCB- —unstained). Subsequently, COCs were matured for 22 h. Nuclear maturation was evaluated at 22 h of culture. Results: The proportion of BCB- oocytes was higher in the winter of 2010, but there was no increase in this group in the winter of 2011. The percentage of oocytes that reached metaphase II was higher in control and BCB+ groups in relation to oocytes from BCB- group. Conclusion: The season of the year influences the percentage of oocytes best suited for embryonic production in situations in which oocyte donors receive pasture-based feeding, since the method was effective in determining the effect of seasonality on the competence of bovine oocytes to reach nuclear maturation. © 2017, Universidad de Antioquia. All rights reserved. |
publishDate |
2023 |
dc.date.issued.none.fl_str_mv |
01/10/2017 |
dc.date.accessioned.none.fl_str_mv |
2023-05-24T16:21:34Z |
dc.date.available.none.fl_str_mv |
2023-05-24T16:21:34Z |
dc.type.none.fl_str_mv |
Artículo |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.redcol.none.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
dc.type.version.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://doi.org/10.17533/udea.rccp.v30n4a01 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031127791&doi=10.17533%2fudea.rccp.v30n4a01&partnerID=40&md5=06b7fd472cb906da1a723217d9762a22 |
dc.identifier.issn.spa.fl_str_mv |
01200690 |
dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12494/49621 |
dc.identifier.bibliographicCitation.spa.fl_str_mv |
Maia R.C.,Mogollon Waltero Edgar mauricio,Dubeibe Marin Diego fernando,Carvalho B.P.,Dias Á.J.B..Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method.Revista Colombiana De Ciencias Pecuarias. 2017. 30. (4):p. 259-266 |
url |
https://doi.org/10.17533/udea.rccp.v30n4a01 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031127791&doi=10.17533%2fudea.rccp.v30n4a01&partnerID=40&md5=06b7fd472cb906da1a723217d9762a22 https://hdl.handle.net/20.500.12494/49621 |
identifier_str_mv |
01200690 Maia R.C.,Mogollon Waltero Edgar mauricio,Dubeibe Marin Diego fernando,Carvalho B.P.,Dias Á.J.B..Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method.Revista Colombiana De Ciencias Pecuarias. 2017. 30. (4):p. 259-266 |
dc.relation.ispartofjournal.spa.fl_str_mv |
Revista Colombiana De Ciencias Pecuarias |
dc.rights.accessrights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.coar.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.format.extent.spa.fl_str_mv |
259-266 |
dc.publisher.spa.fl_str_mv |
Universidad de Antioquia |
institution |
Universidad Cooperativa de Colombia |
repository.name.fl_str_mv |
Repositorio Institucional Universidad Cooperativa de Colombia |
repository.mail.fl_str_mv |
bdigital@metabiblioteca.com |
_version_ |
1814247245132857344 |