Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method

Background: The brilliant cresyl blue (BCB) staining is a non-invasive test to select the best-suited oocytes for embryonic development. This makes it a useful tool to select best-quality oocytes at the times of the year when there is forage restriction. Objective: To evaluate the effect of seasonal...

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Autores:
Maia R.C.
Mogollon Waltero, Edgar mauricio
Dubeibe Marin, Diego fernando
Carvalho B.P.
Dias Á.J.B.
Tipo de recurso:
Article of journal
Fecha de publicación:
2023
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/49621
Acceso en línea:
https://doi.org/10.17533/udea.rccp.v30n4a01
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031127791&doi=10.17533%2fudea.rccp.v30n4a01&partnerID=40&md5=06b7fd472cb906da1a723217d9762a22
https://hdl.handle.net/20.500.12494/49621
Palabra clave:
COLORIMETRIC TEST
CUMULUS-OOPHORUS COMPLEXES
IN VITRO MATURATION
PASTURE
SEASONS
Rights
openAccess
License
http://purl.org/coar/access_right/c_abf2
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oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/49621
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
spelling Maia R.C.Mogollon Waltero, Edgar mauricioDubeibe Marin, Diego fernandoCarvalho B.P.Dias Á.J.B.2023-05-24T16:21:34Z2023-05-24T16:21:34Z01/10/2017https://doi.org/10.17533/udea.rccp.v30n4a01https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031127791&doi=10.17533%2fudea.rccp.v30n4a01&partnerID=40&md5=06b7fd472cb906da1a723217d9762a2201200690https://hdl.handle.net/20.500.12494/49621Maia R.C.,Mogollon Waltero Edgar mauricio,Dubeibe Marin Diego fernando,Carvalho B.P.,Dias Á.J.B..Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method.Revista Colombiana De Ciencias Pecuarias. 2017. 30. (4):p. 259-266Background: The brilliant cresyl blue (BCB) staining is a non-invasive test to select the best-suited oocytes for embryonic development. This makes it a useful tool to select best-quality oocytes at the times of the year when there is forage restriction. Objective: To evaluate the effect of seasonality on the nuclear maturation and quality of oocytes selected by the BCB test. Methods: The cumulus-oophorus complexes (COCs) were obtained in summer and winter of 2010 and 2011. Selected COCs were maintained for 90 min at 38.5 °C in a CO2 incubator, in TCM 199 medium containing 10% fetal bovine serum and antibiotics, and supplemented with 26 µM brilliant cresyl blue. Afterwards, they were divided according to the ooplasm staining (BCB+ —blue; BCB- —unstained). Subsequently, COCs were matured for 22 h. Nuclear maturation was evaluated at 22 h of culture. Results: The proportion of BCB- oocytes was higher in the winter of 2010, but there was no increase in this group in the winter of 2011. The percentage of oocytes that reached metaphase II was higher in control and BCB+ groups in relation to oocytes from BCB- group. Conclusion: The season of the year influences the percentage of oocytes best suited for embryonic production in situations in which oocyte donors receive pasture-based feeding, since the method was effective in determining the effect of seasonality on the competence of bovine oocytes to reach nuclear maturation. © 2017, Universidad de Antioquia. All rights reserved.edgar.mogollonw@campusucc.edu.co259-266Universidad de AntioquiaCOLORIMETRIC TESTCUMULUS-OOPHORUS COMPLEXESIN VITRO MATURATIONPASTURESEASONSEffect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue methodArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionRevista Colombiana De Ciencias Pecuariasinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Publication20.500.12494/49621oai:repository.ucc.edu.co:20.500.12494/496212024-08-20 16:22:23.778metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method
title Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method
spellingShingle Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method
COLORIMETRIC TEST
CUMULUS-OOPHORUS COMPLEXES
IN VITRO MATURATION
PASTURE
SEASONS
title_short Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method
title_full Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method
title_fullStr Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method
title_full_unstemmed Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method
title_sort Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method
dc.creator.fl_str_mv Maia R.C.
Mogollon Waltero, Edgar mauricio
Dubeibe Marin, Diego fernando
Carvalho B.P.
Dias Á.J.B.
dc.contributor.author.none.fl_str_mv Maia R.C.
Mogollon Waltero, Edgar mauricio
Dubeibe Marin, Diego fernando
Carvalho B.P.
Dias Á.J.B.
dc.subject.spa.fl_str_mv COLORIMETRIC TEST
CUMULUS-OOPHORUS COMPLEXES
IN VITRO MATURATION
PASTURE
SEASONS
topic COLORIMETRIC TEST
CUMULUS-OOPHORUS COMPLEXES
IN VITRO MATURATION
PASTURE
SEASONS
description Background: The brilliant cresyl blue (BCB) staining is a non-invasive test to select the best-suited oocytes for embryonic development. This makes it a useful tool to select best-quality oocytes at the times of the year when there is forage restriction. Objective: To evaluate the effect of seasonality on the nuclear maturation and quality of oocytes selected by the BCB test. Methods: The cumulus-oophorus complexes (COCs) were obtained in summer and winter of 2010 and 2011. Selected COCs were maintained for 90 min at 38.5 °C in a CO2 incubator, in TCM 199 medium containing 10% fetal bovine serum and antibiotics, and supplemented with 26 µM brilliant cresyl blue. Afterwards, they were divided according to the ooplasm staining (BCB+ —blue; BCB- —unstained). Subsequently, COCs were matured for 22 h. Nuclear maturation was evaluated at 22 h of culture. Results: The proportion of BCB- oocytes was higher in the winter of 2010, but there was no increase in this group in the winter of 2011. The percentage of oocytes that reached metaphase II was higher in control and BCB+ groups in relation to oocytes from BCB- group. Conclusion: The season of the year influences the percentage of oocytes best suited for embryonic production in situations in which oocyte donors receive pasture-based feeding, since the method was effective in determining the effect of seasonality on the competence of bovine oocytes to reach nuclear maturation. © 2017, Universidad de Antioquia. All rights reserved.
publishDate 2023
dc.date.issued.none.fl_str_mv 01/10/2017
dc.date.accessioned.none.fl_str_mv 2023-05-24T16:21:34Z
dc.date.available.none.fl_str_mv 2023-05-24T16:21:34Z
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
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dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.17533/udea.rccp.v30n4a01
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031127791&doi=10.17533%2fudea.rccp.v30n4a01&partnerID=40&md5=06b7fd472cb906da1a723217d9762a22
dc.identifier.issn.spa.fl_str_mv 01200690
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/49621
dc.identifier.bibliographicCitation.spa.fl_str_mv Maia R.C.,Mogollon Waltero Edgar mauricio,Dubeibe Marin Diego fernando,Carvalho B.P.,Dias Á.J.B..Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method.Revista Colombiana De Ciencias Pecuarias. 2017. 30. (4):p. 259-266
url https://doi.org/10.17533/udea.rccp.v30n4a01
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031127791&doi=10.17533%2fudea.rccp.v30n4a01&partnerID=40&md5=06b7fd472cb906da1a723217d9762a22
https://hdl.handle.net/20.500.12494/49621
identifier_str_mv 01200690
Maia R.C.,Mogollon Waltero Edgar mauricio,Dubeibe Marin Diego fernando,Carvalho B.P.,Dias Á.J.B..Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method.Revista Colombiana De Ciencias Pecuarias. 2017. 30. (4):p. 259-266
dc.relation.ispartofjournal.spa.fl_str_mv Revista Colombiana De Ciencias Pecuarias
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_abf2
dc.format.extent.spa.fl_str_mv 259-266
dc.publisher.spa.fl_str_mv Universidad de Antioquia
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
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