Effect of high doses of vitamin D supplementation on dengue virus replication, Toll-like receptor expression, and cytokine profiles on dendritic cells

Dengue, caused by dengue virus (DENV) infection, is a public health problem worldwide. Although DENV pathogenesis has not yet been fully elucidated, the inflammatory response is a hallmark feature in severe DENV infection. Although vitamin D (vitD) can promote the innate immune response against viru...

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Autores:
Martínez Moreno, Jahnnyer
Hernández López, Juan Carlos
Urcuqui Inchima, Silvio
Tipo de recurso:
Article of journal
Fecha de publicación:
2020
Institución:
Universidad Cooperativa de Colombia
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Repositorio UCC
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OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/15938
Acceso en línea:
https://hdl.handle.net/20.500.12494/15938
Palabra clave:
Inflammation
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openAccess
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oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/15938
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dc.title.spa.fl_str_mv Effect of high doses of vitamin D supplementation on dengue virus replication, Toll-like receptor expression, and cytokine profiles on dendritic cells
title Effect of high doses of vitamin D supplementation on dengue virus replication, Toll-like receptor expression, and cytokine profiles on dendritic cells
spellingShingle Effect of high doses of vitamin D supplementation on dengue virus replication, Toll-like receptor expression, and cytokine profiles on dendritic cells
Inflammation
title_short Effect of high doses of vitamin D supplementation on dengue virus replication, Toll-like receptor expression, and cytokine profiles on dendritic cells
title_full Effect of high doses of vitamin D supplementation on dengue virus replication, Toll-like receptor expression, and cytokine profiles on dendritic cells
title_fullStr Effect of high doses of vitamin D supplementation on dengue virus replication, Toll-like receptor expression, and cytokine profiles on dendritic cells
title_full_unstemmed Effect of high doses of vitamin D supplementation on dengue virus replication, Toll-like receptor expression, and cytokine profiles on dendritic cells
title_sort Effect of high doses of vitamin D supplementation on dengue virus replication, Toll-like receptor expression, and cytokine profiles on dendritic cells
dc.creator.fl_str_mv Martínez Moreno, Jahnnyer
Hernández López, Juan Carlos
Urcuqui Inchima, Silvio
dc.contributor.author.none.fl_str_mv Martínez Moreno, Jahnnyer
Hernández López, Juan Carlos
Urcuqui Inchima, Silvio
dc.subject.spa.fl_str_mv Inflammation
topic Inflammation
description Dengue, caused by dengue virus (DENV) infection, is a public health problem worldwide. Although DENV pathogenesis has not yet been fully elucidated, the inflammatory response is a hallmark feature in severe DENV infection. Although vitamin D (vitD) can promote the innate immune response against virus infection, no studies have evaluated the effects of vitD on DENV infection, dendritic cells (DCs), and inflammatory response regulation. This study aimed to assess the impact of oral vitD supplementation on DENV-2 infection, Toll-like receptor (TLR) expression, and both pro- and anti-inflammatory cytokine production in monocyte-derived DCs (MDDCs). To accomplish this, 20 healthy donors were randomly divided into two groups and received either 1000 or 4000 international units (IU)/day of vitD for 10 days. During pre- and post-vitD supplementation, peripheral blood samples were taken to obtain MDDCs, which were challenged with DENV-2. We found that MDDCs from donors who received 4000 IU/day of vitD were less susceptible to DENV-2 infection than MDDCs from donors who received 1000 IU/day of vitD. Moreover, these cells showed decreased mRNA expression of TLR3, 7, and 9; downregulation of IL-12/IL-8 production; and increased IL-10 secretion in response to DENV-2 infection. In conclusion, the administration of 4000 IU/day of vitD decreased DENV-2 infection. Our findings support a possible role of vitD in improving the innate immune response against DENV. However, further studies are necessary to determine the role of vitD on DENV replication and its innate immune response modulation in MDDCs.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-01-15T18:48:20Z
dc.date.available.none.fl_str_mv 2020-01-15T18:48:20Z
dc.date.issued.none.fl_str_mv 2020-01-02
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
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dc.identifier.issn.spa.fl_str_mv 1573-4919
dc.identifier.uri.spa.fl_str_mv 10.1007/s11010-019-03658-w
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/15938
dc.identifier.bibliographicCitation.spa.fl_str_mv Martínez-Moreno, J., Hernandez, J.C. & Urcuqui-Inchima, S. (2019). Effect of high doses of vitamin D supplementation on dengue virus replication, Toll-like receptor expression, and cytokine profiles on dendritic cells. Mol Cell Biochem (2020) 464: 169. https://doi.org/10.1007/s11010-019-03658-w. Recuperado de: http://hdl.handle.net/20.500.12494/15938
identifier_str_mv 1573-4919
10.1007/s11010-019-03658-w
Martínez-Moreno, J., Hernandez, J.C. & Urcuqui-Inchima, S. (2019). Effect of high doses of vitamin D supplementation on dengue virus replication, Toll-like receptor expression, and cytokine profiles on dendritic cells. Mol Cell Biochem (2020) 464: 169. https://doi.org/10.1007/s11010-019-03658-w. Recuperado de: http://hdl.handle.net/20.500.12494/15938
url https://hdl.handle.net/20.500.12494/15938
dc.relation.isversionof.spa.fl_str_mv https://link.springer.com/article/10.1007%2Fs11010-019-03658-w#citeas
dc.relation.ispartofjournal.spa.fl_str_mv Molecular and Cellular Biochemistry
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spelling Martínez Moreno, JahnnyerHernández López, Juan CarlosUrcuqui Inchima, Silvio464 (1–2)2020-01-15T18:48:20Z2020-01-15T18:48:20Z2020-01-021573-491910.1007/s11010-019-03658-whttps://hdl.handle.net/20.500.12494/15938Martínez-Moreno, J., Hernandez, J.C. & Urcuqui-Inchima, S. (2019). Effect of high doses of vitamin D supplementation on dengue virus replication, Toll-like receptor expression, and cytokine profiles on dendritic cells. Mol Cell Biochem (2020) 464: 169. https://doi.org/10.1007/s11010-019-03658-w. Recuperado de: http://hdl.handle.net/20.500.12494/15938Dengue, caused by dengue virus (DENV) infection, is a public health problem worldwide. Although DENV pathogenesis has not yet been fully elucidated, the inflammatory response is a hallmark feature in severe DENV infection. Although vitamin D (vitD) can promote the innate immune response against virus infection, no studies have evaluated the effects of vitD on DENV infection, dendritic cells (DCs), and inflammatory response regulation. This study aimed to assess the impact of oral vitD supplementation on DENV-2 infection, Toll-like receptor (TLR) expression, and both pro- and anti-inflammatory cytokine production in monocyte-derived DCs (MDDCs). To accomplish this, 20 healthy donors were randomly divided into two groups and received either 1000 or 4000 international units (IU)/day of vitD for 10 days. During pre- and post-vitD supplementation, peripheral blood samples were taken to obtain MDDCs, which were challenged with DENV-2. We found that MDDCs from donors who received 4000 IU/day of vitD were less susceptible to DENV-2 infection than MDDCs from donors who received 1000 IU/day of vitD. Moreover, these cells showed decreased mRNA expression of TLR3, 7, and 9; downregulation of IL-12/IL-8 production; and increased IL-10 secretion in response to DENV-2 infection. In conclusion, the administration of 4000 IU/day of vitD decreased DENV-2 infection. Our findings support a possible role of vitD in improving the innate immune response against DENV. However, further studies are necessary to determine the role of vitD on DENV replication and its innate immune response modulation in MDDCs.https://scienti.colciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000283088http://orcid.org/0000-0002-9200-5698https://scienti.colciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000011355juanc.hernandezl@campusucc.edu.co169–180Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Medellín y Envigado, Colombia, 00000MedicinaMedellínhttps://link.springer.com/article/10.1007%2Fs11010-019-03658-w#citeasMolecular and Cellular BiochemistryBhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, Drake JM, Brownstein JS, Hoen AG, Sankoh O, Myers MF, George DB, Jaenisch T, Wint GR, Simmons CP, Scott TW, Farrar JJ, Hay SI (2013) The global distribution and burden of dengue. Nature 496(7446):504–507. https ://doi.org/10.1038/natur e1206 0Guzman MG, Harris E (2014) Dengue. 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Int Immunopharmacol 15(1):15–22. https: //doi.org/10.1016/j.intim p.2012.10.027InflammationEffect of high doses of vitamin D supplementation on dengue virus replication, Toll-like receptor expression, and cytokine profiles on dendritic cellsArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribucióninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-84334https://repository.ucc.edu.co/bitstreams/5dc5e71b-e67e-4888-ab3e-430518888ca6/download3bce4f7ab09dfc588f126e1e36e98a45MD52ORIGINALVit Suppl Dengue MCB 2020.pdfVit Suppl Dengue MCB 2020.pdfArtículoapplication/pdf1336331https://repository.ucc.edu.co/bitstreams/bad3d443-4e4a-464e-8131-00d12d7130fa/download040c7b78b4b6311852a62a558b8e77d2MD51THUMBNAILVit Suppl Dengue MCB 2020.pdf.jpgVit Suppl 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