Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1

Defensins, RNases and cytokines are present at mucosal barriers, main ports of HIV entry, and are potential mediators of the resistant phenotype exhibited by HIV-1-exposed seronegative individuals (HESN) during sexual exposure. We aimed to determine the role of soluble factors in natural resistance...

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Autores:
Zapata Builes, Wildeman
Aguilar-Jiménez W.
Feng Z.
Weinberg A.
Russo A.
Potenza N.
Estrada H.
Rugeles M.T.
Tipo de recurso:
Article of journal
Fecha de publicación:
2023
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/49574
Acceso en línea:
https://doi.org/10.1016/j.micinf.2015.10.009
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84975735297&doi=10.1016%2fj.micinf.2015.10.009&partnerID=40&md5=580900d00e9d18136bc5ab9feb5b4cb9
https://hdl.handle.net/20.500.12494/49574
Palabra clave:
ADOLESCENT
ADULT
ANGIOGENIN
ANTIVIRAL ACTIVITY
ARTICLE
BETA DEFENSIN 2
BETA DEFENSIN 3
CD4+ T LYMPHOCYTE
CONTROLLED STUDY
DISEASE RESISTANCE
DOSE RESPONSE
FEMA
HUMAN
HUMAN IMMUNODEFICIENCY VIRUS 1
HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION
IMMUNOLOGIC FACTOR
IN VITRO STUDY
IN VIVO STUDY
INNATE IMMUNITY
LIFE CYCLE
MACROPHAGE INFLAMMATORY PROTEIN 1BETA
MAJOR CLINICAL STUDY
MARAVIROC
MESSENGER RNA
MOUTH MUCOSA
N [4 [[[6,7 DIHYDRO 2 (4 METHYLPHENYL) 5H BENZOCYCLOHEPTEN 8 YL]CARBONYL]AMINO]BENZYL] N,N DIMETHYL 2H TETRAHYDROPYRAN 4 AMINIUM CHLORIDE
NEUTROPHIL PEPTIDE 1
PLERIXAFOR
POLYMERASE CHAIN REACTION
PRIORITY JOURNAL
PROTEIN
PROTEIN ANALYSIS
PROTEIN EXPRESSION
PROTEIN FUNCTION
QUANTITATIVE ANALYSIS
RANTES
REVERSE TRANSCRIPTION
RIBONUCLEASE
SEXUAL INTERCOURSE
UNCLASSIFIED DRUG
UTERINE CERVIX MUCOSA
VAGINA MUCOSA
VIRAL TROPISM
VIRUS ENTRY
VIRUS INFECTIVITY
VIRUS INHIBITION
VIRUS LOAD
VIRUS REPLICATION
VIRUS RESISTANCE
VIRUS STRAIN
ZIDOVUDINE
Rights
openAccess
License
http://purl.org/coar/access_right/c_abf2
id COOPER2_52895a87d93ffc84ee4657806e2f2b4f
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/49574
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
spelling Zapata Builes, WildemanAguilar-Jiménez W.Feng Z.Weinberg A.Russo A.Potenza N.Estrada H.Rugeles M.T.2023-05-24T16:21:32Z2023-05-24T16:21:32Z01/01/2016https://doi.org/10.1016/j.micinf.2015.10.009https://www.scopus.com/inward/record.uri?eid=2-s2.0-84975735297&doi=10.1016%2fj.micinf.2015.10.009&partnerID=40&md5=580900d00e9d18136bc5ab9feb5b4cb912864579https://hdl.handle.net/20.500.12494/49574Zapata Builes Wildeman,Aguilar-Jiménez W.,Feng Z.,Weinberg A.,Russo A.,Potenza N.,Estrada H.,Rugeles M.T..Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1.MICROBES AND INFECTION. 2016. 18. (3):p. 211-219Defensins, RNases and cytokines are present at mucosal barriers, main ports of HIV entry, and are potential mediators of the resistant phenotype exhibited by HIV-1-exposed seronegative individuals (HESN) during sexual exposure. We aimed to determine the role of soluble factors in natural resistance to HIV-1 infection. Vaginal/endocervical/oral mucosal samples were taken from 60 HESN, 60 seropositive (SP) and 61 healthy controls (HC). Human neutrophil peptide 1 (hNP-1), human beta defensin (hBD) 2 and 3, RNases, MIP-1ß and RANTES mRNA transcripts were quantified by qPCR and in vitro single-round, recombinant-based viral infectivity assay was used to evaluate the anti-HIV-1 activity of hBDs and RNases. HESN expressed significantly higher levels of hNP-1, hBDs mRNA in oral mucosa compared to HC (P < 0.05). In genital mucosa, significantly higher mRNA levels of MIP-1ß, RANTES and RNases were found in HESN compared to HC (P < 0.05). HBDs and RNases inhibit HIV-1 replication, particularly R5 at entry, reverse transcription and nuclear import of the viral life cycle. hNP-1, hBDs, MIP-1ß, RANTES and RNases, collectively could contribute to HIV-1 resistance during sexual exposure. Moreover, the inhibition of HIV-1 infection in vitro by hBDs and RNases suggests that they may be exploited as potential antiretrovirals. © 2015 Institut Pasteur.0000-0002-7351-8738wildeman.zapatab@campusucc.edu.co211-219Elsevier Masson SASADOLESCENTADULTANGIOGENINANTIVIRAL ACTIVITYARTICLEBETA DEFENSIN 2BETA DEFENSIN 3CD4+ T LYMPHOCYTECONTROLLED STUDYDISEASE RESISTANCEDOSE RESPONSEFEMAHUMANHUMAN IMMUNODEFICIENCY VIRUS 1HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTIONIMMUNOLOGIC FACTORIN VITRO STUDYIN VIVO STUDYINNATE IMMUNITYLIFE CYCLEMACROPHAGE INFLAMMATORY PROTEIN 1BETAMAJOR CLINICAL STUDYMARAVIROCMESSENGER RNAMOUTH MUCOSAN [4 [[[6,7 DIHYDRO 2 (4 METHYLPHENYL) 5H BENZOCYCLOHEPTEN 8 YL]CARBONYL]AMINO]BENZYL] N,N DIMETHYL 2H TETRAHYDROPYRAN 4 AMINIUM CHLORIDENEUTROPHIL PEPTIDE 1PLERIXAFORPOLYMERASE CHAIN REACTIONPRIORITY JOURNALPROTEINPROTEIN ANALYSISPROTEIN EXPRESSIONPROTEIN FUNCTIONQUANTITATIVE ANALYSISRANTESREVERSE TRANSCRIPTIONRIBONUCLEASESEXUAL INTERCOURSEUNCLASSIFIED DRUGUTERINE CERVIX MUCOSAVAGINA MUCOSAVIRAL TROPISMVIRUS ENTRYVIRUS INFECTIVITYVIRUS INHIBITIONVIRUS LOADVIRUS REPLICATIONVIRUS RESISTANCEVIRUS STRAINZIDOVUDINEIdentification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1Artículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionMICROBES AND INFECTIONinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Publication20.500.12494/49574oai:repository.ucc.edu.co:20.500.12494/495742024-08-20 16:17:52.002metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
title Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
spellingShingle Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
ADOLESCENT
ADULT
ANGIOGENIN
ANTIVIRAL ACTIVITY
ARTICLE
BETA DEFENSIN 2
BETA DEFENSIN 3
CD4+ T LYMPHOCYTE
CONTROLLED STUDY
DISEASE RESISTANCE
DOSE RESPONSE
FEMA
HUMAN
HUMAN IMMUNODEFICIENCY VIRUS 1
HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION
IMMUNOLOGIC FACTOR
IN VITRO STUDY
IN VIVO STUDY
INNATE IMMUNITY
LIFE CYCLE
MACROPHAGE INFLAMMATORY PROTEIN 1BETA
MAJOR CLINICAL STUDY
MARAVIROC
MESSENGER RNA
MOUTH MUCOSA
N [4 [[[6,7 DIHYDRO 2 (4 METHYLPHENYL) 5H BENZOCYCLOHEPTEN 8 YL]CARBONYL]AMINO]BENZYL] N,N DIMETHYL 2H TETRAHYDROPYRAN 4 AMINIUM CHLORIDE
NEUTROPHIL PEPTIDE 1
PLERIXAFOR
POLYMERASE CHAIN REACTION
PRIORITY JOURNAL
PROTEIN
PROTEIN ANALYSIS
PROTEIN EXPRESSION
PROTEIN FUNCTION
QUANTITATIVE ANALYSIS
RANTES
REVERSE TRANSCRIPTION
RIBONUCLEASE
SEXUAL INTERCOURSE
UNCLASSIFIED DRUG
UTERINE CERVIX MUCOSA
VAGINA MUCOSA
VIRAL TROPISM
VIRUS ENTRY
VIRUS INFECTIVITY
VIRUS INHIBITION
VIRUS LOAD
VIRUS REPLICATION
VIRUS RESISTANCE
VIRUS STRAIN
ZIDOVUDINE
title_short Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
title_full Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
title_fullStr Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
title_full_unstemmed Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
title_sort Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
dc.creator.fl_str_mv Zapata Builes, Wildeman
Aguilar-Jiménez W.
Feng Z.
Weinberg A.
Russo A.
Potenza N.
Estrada H.
Rugeles M.T.
dc.contributor.author.none.fl_str_mv Zapata Builes, Wildeman
Aguilar-Jiménez W.
Feng Z.
Weinberg A.
Russo A.
Potenza N.
Estrada H.
Rugeles M.T.
dc.subject.spa.fl_str_mv ADOLESCENT
ADULT
ANGIOGENIN
ANTIVIRAL ACTIVITY
ARTICLE
BETA DEFENSIN 2
BETA DEFENSIN 3
CD4+ T LYMPHOCYTE
CONTROLLED STUDY
DISEASE RESISTANCE
DOSE RESPONSE
FEMA
HUMAN
HUMAN IMMUNODEFICIENCY VIRUS 1
HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION
IMMUNOLOGIC FACTOR
IN VITRO STUDY
IN VIVO STUDY
INNATE IMMUNITY
LIFE CYCLE
MACROPHAGE INFLAMMATORY PROTEIN 1BETA
MAJOR CLINICAL STUDY
MARAVIROC
MESSENGER RNA
MOUTH MUCOSA
N [4 [[[6,7 DIHYDRO 2 (4 METHYLPHENYL) 5H BENZOCYCLOHEPTEN 8 YL]CARBONYL]AMINO]BENZYL] N,N DIMETHYL 2H TETRAHYDROPYRAN 4 AMINIUM CHLORIDE
NEUTROPHIL PEPTIDE 1
PLERIXAFOR
POLYMERASE CHAIN REACTION
PRIORITY JOURNAL
PROTEIN
PROTEIN ANALYSIS
PROTEIN EXPRESSION
PROTEIN FUNCTION
QUANTITATIVE ANALYSIS
RANTES
REVERSE TRANSCRIPTION
RIBONUCLEASE
SEXUAL INTERCOURSE
UNCLASSIFIED DRUG
UTERINE CERVIX MUCOSA
VAGINA MUCOSA
VIRAL TROPISM
VIRUS ENTRY
VIRUS INFECTIVITY
VIRUS INHIBITION
VIRUS LOAD
VIRUS REPLICATION
VIRUS RESISTANCE
VIRUS STRAIN
ZIDOVUDINE
topic ADOLESCENT
ADULT
ANGIOGENIN
ANTIVIRAL ACTIVITY
ARTICLE
BETA DEFENSIN 2
BETA DEFENSIN 3
CD4+ T LYMPHOCYTE
CONTROLLED STUDY
DISEASE RESISTANCE
DOSE RESPONSE
FEMA
HUMAN
HUMAN IMMUNODEFICIENCY VIRUS 1
HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION
IMMUNOLOGIC FACTOR
IN VITRO STUDY
IN VIVO STUDY
INNATE IMMUNITY
LIFE CYCLE
MACROPHAGE INFLAMMATORY PROTEIN 1BETA
MAJOR CLINICAL STUDY
MARAVIROC
MESSENGER RNA
MOUTH MUCOSA
N [4 [[[6,7 DIHYDRO 2 (4 METHYLPHENYL) 5H BENZOCYCLOHEPTEN 8 YL]CARBONYL]AMINO]BENZYL] N,N DIMETHYL 2H TETRAHYDROPYRAN 4 AMINIUM CHLORIDE
NEUTROPHIL PEPTIDE 1
PLERIXAFOR
POLYMERASE CHAIN REACTION
PRIORITY JOURNAL
PROTEIN
PROTEIN ANALYSIS
PROTEIN EXPRESSION
PROTEIN FUNCTION
QUANTITATIVE ANALYSIS
RANTES
REVERSE TRANSCRIPTION
RIBONUCLEASE
SEXUAL INTERCOURSE
UNCLASSIFIED DRUG
UTERINE CERVIX MUCOSA
VAGINA MUCOSA
VIRAL TROPISM
VIRUS ENTRY
VIRUS INFECTIVITY
VIRUS INHIBITION
VIRUS LOAD
VIRUS REPLICATION
VIRUS RESISTANCE
VIRUS STRAIN
ZIDOVUDINE
description Defensins, RNases and cytokines are present at mucosal barriers, main ports of HIV entry, and are potential mediators of the resistant phenotype exhibited by HIV-1-exposed seronegative individuals (HESN) during sexual exposure. We aimed to determine the role of soluble factors in natural resistance to HIV-1 infection. Vaginal/endocervical/oral mucosal samples were taken from 60 HESN, 60 seropositive (SP) and 61 healthy controls (HC). Human neutrophil peptide 1 (hNP-1), human beta defensin (hBD) 2 and 3, RNases, MIP-1ß and RANTES mRNA transcripts were quantified by qPCR and in vitro single-round, recombinant-based viral infectivity assay was used to evaluate the anti-HIV-1 activity of hBDs and RNases. HESN expressed significantly higher levels of hNP-1, hBDs mRNA in oral mucosa compared to HC (P < 0.05). In genital mucosa, significantly higher mRNA levels of MIP-1ß, RANTES and RNases were found in HESN compared to HC (P < 0.05). HBDs and RNases inhibit HIV-1 replication, particularly R5 at entry, reverse transcription and nuclear import of the viral life cycle. hNP-1, hBDs, MIP-1ß, RANTES and RNases, collectively could contribute to HIV-1 resistance during sexual exposure. Moreover, the inhibition of HIV-1 infection in vitro by hBDs and RNases suggests that they may be exploited as potential antiretrovirals. © 2015 Institut Pasteur.
publishDate 2023
dc.date.issued.none.fl_str_mv 01/01/2016
dc.date.accessioned.none.fl_str_mv 2023-05-24T16:21:32Z
dc.date.available.none.fl_str_mv 2023-05-24T16:21:32Z
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.micinf.2015.10.009
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84975735297&doi=10.1016%2fj.micinf.2015.10.009&partnerID=40&md5=580900d00e9d18136bc5ab9feb5b4cb9
dc.identifier.issn.spa.fl_str_mv 12864579
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/49574
dc.identifier.bibliographicCitation.spa.fl_str_mv Zapata Builes Wildeman,Aguilar-Jiménez W.,Feng Z.,Weinberg A.,Russo A.,Potenza N.,Estrada H.,Rugeles M.T..Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1.MICROBES AND INFECTION. 2016. 18. (3):p. 211-219
url https://doi.org/10.1016/j.micinf.2015.10.009
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84975735297&doi=10.1016%2fj.micinf.2015.10.009&partnerID=40&md5=580900d00e9d18136bc5ab9feb5b4cb9
https://hdl.handle.net/20.500.12494/49574
identifier_str_mv 12864579
Zapata Builes Wildeman,Aguilar-Jiménez W.,Feng Z.,Weinberg A.,Russo A.,Potenza N.,Estrada H.,Rugeles M.T..Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1.MICROBES AND INFECTION. 2016. 18. (3):p. 211-219
dc.relation.ispartofjournal.spa.fl_str_mv MICROBES AND INFECTION
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_abf2
dc.format.extent.spa.fl_str_mv 211-219
dc.publisher.spa.fl_str_mv Elsevier Masson SAS
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
_version_ 1814246801134321664

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