Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1

Defensins, RNases and cytokines are present at mucosal barriers, main ports of HIV entry, and are potential mediators of the resistant phenotype exhibited by HIV-1-exposed seronegative individuals (HESN) during sexual exposure. We aimed to determine the role of soluble factors in natural resistance to HIV-1 infection. Vaginal/endocervical/oral mucosal samples were taken from 60 HESN, 60 seropositive (SP) and 61 healthy controls (HC). Human neutrophil peptide 1 (hNP-1), human beta defensin (hBD) 2 and 3, RNases, MIP-1ß and RANTES mRNA transcripts were quantified by qPCR and in vitro single-round, recombinant-based viral infectivity assay was used to evaluate the anti-HIV-1 activity of hBDs and RNases. HESN expressed significantly higher levels of hNP-1, hBDs mRNA in oral mucosa compared to HC (P < 0.05). In genital mucosa, significantly higher mRNA levels of MIP-1ß, RANTES and RNases were found in HESN compared to HC (P < 0.05). HBDs and RNases inhibit HIV-1 replication, particularly R5 at entry, reverse transcription and nuclear import of the viral life cycle. hNP-1, hBDs, MIP-1ß, RANTES and RNases, collectively could contribute to HIV-1 resistance during sexual exposure. Moreover, the inhibition of HIV-1 infection in vitro by hBDs and RNases suggests that they may be exploited as potential antiretrovirals. © 2015 Institut Pasteur.

• Autores: Zapata Builes, Wildeman; Aguilar-Jiménez W.; Feng Z.; Weinberg A.; Russo A.; Potenza N.; Estrada H.; Rugeles M.T.
• Tipo de recurso: Article of journal
• Fecha de publicación: 2023
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/49574
• Acceso en línea: https://doi.org/10.1016/j.micinf.2015.10.009; https://www.scopus.com/inward/record.uri?eid=2-s2.0-84975735297&doi=10.1016%2fj.micinf.2015.10.009&partnerID=40&md5=580900d00e9d18136bc5ab9feb5b4cb9; https://hdl.handle.net/20.500.12494/49574
• Palabra clave: ADOLESCENT; ADULT; ANGIOGENIN; ANTIVIRAL ACTIVITY; ARTICLE; BETA DEFENSIN 2; BETA DEFENSIN 3; CD4+ T LYMPHOCYTE; CONTROLLED STUDY; DISEASE RESISTANCE; DOSE RESPONSE; FEMA; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; IMMUNOLOGIC FACTOR; IN VITRO STUDY; IN VIVO STUDY; INNATE IMMUNITY; LIFE CYCLE; MACROPHAGE INFLAMMATORY PROTEIN 1BETA; MAJOR CLINICAL STUDY; MARAVIROC; MESSENGER RNA; MOUTH MUCOSA; N [4 [[[6,7 DIHYDRO 2 (4 METHYLPHENYL) 5H BENZOCYCLOHEPTEN 8 YL]CARBONYL]AMINO]BENZYL] N,N DIMETHYL 2H TETRAHYDROPYRAN 4 AMINIUM CHLORIDE; NEUTROPHIL PEPTIDE 1; PLERIXAFOR; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; QUANTITATIVE ANALYSIS; RANTES; REVERSE TRANSCRIPTION; RIBONUCLEASE; SEXUAL INTERCOURSE; UNCLASSIFIED DRUG; UTERINE CERVIX MUCOSA; VAGINA MUCOSA; VIRAL TROPISM; VIRUS ENTRY; VIRUS INFECTIVITY; VIRUS INHIBITION; VIRUS LOAD; VIRUS REPLICATION; VIRUS RESISTANCE; VIRUS STRAIN; ZIDOVUDINE
• Rights: openAccess
• License: http://purl.org/coar/access_right/c_abf2