Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units

Background: Trypanosoma cruzi is the causative agent of Chagas disease. Due to its genetic diversity has been classified into six Discrete Typing Units (DTUs) in association with transmission cycles. In Colombia, natural T. cruzi infection has been detected in 15 triatomine species. There is scarce...

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Autores:
Hernández C.
Salazar C.
Brochero H.
Teherán A.
Buitrago L.S.
Vera M.
Soto H.
Florez-Rivadeneira Z.
Ardila S.
Parra Henao, Gabriel Jaime
Ramírez J.D.
Tipo de recurso:
Article of journal
Fecha de publicación:
2016
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
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OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/41844
Acceso en línea:
https://doi.org/10.17013/risti.19.15-31
https://revistas.udistrital.edu.co/index.php/cpaz/article/view/11695/12853
https://hdl.handle.net/20.500.12494/41844
Palabra clave:
Article
Chagas disease
Colombia
controlled study
disease transmission
feeding behavior
female
genetic variability
genotype
host parasite interaction
human
limit of quantitation
male
nonhuman
Panstrongylus
Panstrongylus geniculatus
Rhodnius
Rhodnius pallescens
Rhodnius pictipes
Rhodnius prolixus
Triatoma
Triatoma dimidiata
Triatoma maculata
Trypanosoma cruzi
animal
Chagas disease
classification
disease carrier
disease transmission
genetics
isolation and purification
parasitology
prevalence
transmission
Triatominae
Animals
Chagas Disease
Colombia
Disease Transmission
Infectious
Disease Vectors
Genotype
Humans
Prevalence
Triatominae
Trypanosoma cruzi
Rights
closedAccess
License
http://purl.org/coar/access_right/c_14cb
id COOPER2_514c003d79358f0a05421c55e4505031
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/41844
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
dc.title.spa.fl_str_mv Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units
title Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units
spellingShingle Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units
Article
Chagas disease
Colombia
controlled study
disease transmission
feeding behavior
female
genetic variability
genotype
host parasite interaction
human
limit of quantitation
male
nonhuman
Panstrongylus
Panstrongylus geniculatus
Rhodnius
Rhodnius pallescens
Rhodnius pictipes
Rhodnius prolixus
Triatoma
Triatoma dimidiata
Triatoma maculata
Trypanosoma cruzi
animal
Chagas disease
classification
disease carrier
disease transmission
genetics
isolation and purification
parasitology
prevalence
transmission
Triatominae
Animals
Chagas Disease
Colombia
Disease Transmission
Infectious
Disease Vectors
Genotype
Humans
Prevalence
Triatominae
Trypanosoma cruzi
title_short Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units
title_full Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units
title_fullStr Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units
title_full_unstemmed Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units
title_sort Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units
dc.creator.fl_str_mv Hernández C.
Salazar C.
Brochero H.
Teherán A.
Buitrago L.S.
Vera M.
Soto H.
Florez-Rivadeneira Z.
Ardila S.
Parra Henao, Gabriel Jaime
Ramírez J.D.
dc.contributor.author.none.fl_str_mv Hernández C.
Salazar C.
Brochero H.
Teherán A.
Buitrago L.S.
Vera M.
Soto H.
Florez-Rivadeneira Z.
Ardila S.
Parra Henao, Gabriel Jaime
Ramírez J.D.
dc.subject.spa.fl_str_mv Article
Chagas disease
Colombia
controlled study
disease transmission
feeding behavior
female
genetic variability
genotype
host parasite interaction
human
limit of quantitation
male
nonhuman
Panstrongylus
Panstrongylus geniculatus
Rhodnius
Rhodnius pallescens
Rhodnius pictipes
Rhodnius prolixus
Triatoma
Triatoma dimidiata
Triatoma maculata
Trypanosoma cruzi
animal
Chagas disease
classification
disease carrier
disease transmission
genetics
isolation and purification
parasitology
prevalence
transmission
Triatominae
Animals
Chagas Disease
Colombia
Disease Transmission
Infectious
Disease Vectors
Genotype
Humans
Prevalence
Triatominae
Trypanosoma cruzi
topic Article
Chagas disease
Colombia
controlled study
disease transmission
feeding behavior
female
genetic variability
genotype
host parasite interaction
human
limit of quantitation
male
nonhuman
Panstrongylus
Panstrongylus geniculatus
Rhodnius
Rhodnius pallescens
Rhodnius pictipes
Rhodnius prolixus
Triatoma
Triatoma dimidiata
Triatoma maculata
Trypanosoma cruzi
animal
Chagas disease
classification
disease carrier
disease transmission
genetics
isolation and purification
parasitology
prevalence
transmission
Triatominae
Animals
Chagas Disease
Colombia
Disease Transmission
Infectious
Disease Vectors
Genotype
Humans
Prevalence
Triatominae
Trypanosoma cruzi
description Background: Trypanosoma cruzi is the causative agent of Chagas disease. Due to its genetic diversity has been classified into six Discrete Typing Units (DTUs) in association with transmission cycles. In Colombia, natural T. cruzi infection has been detected in 15 triatomine species. There is scarce information regarding the infection rates, DTUs and feeding preferences of secondary vectors. Therefore, the aim of this study was to determine T. cruzi infection rates, parasite DTU, ecotopes, insect stages, geographical location and bug feeding preferences across six different triatomine species. Methods: A total of 245 insects were collected in seven departments of Colombia. We conducted molecular detection and genotyping of T. cruzi with subsequent identification of food sources. The frequency of infection, DTUs, TcI genotypes and feeding sources were plotted across the six species studied. A logistic regression model risk was estimated with insects positive for T. cruzi according to demographic and eco-epidemiological characteristics. Results: We collected 85 specimens of Panstrongylus geniculatus, 77 Rhodnius prolixus, 37 R. pallescens, 34 Triatoma maculata, 8 R. pictipes and 4 T. dimidiata. The overall T. cruzi infection rate was 61.2% and presented statistical associations with the departments Meta (OR: 2.65; 95% CI: 1.69-4.17) and Guajira (OR: 2.13; 95% CI: 1.16-3.94); peridomestic ecotope (OR: 2.52: 95% CI: 1.62-3.93); the vector species P. geniculatus (OR: 2.40; 95% CI: 1.51-3.82) and T. maculata (OR: 2.09; 95% CI: 1.02-4.29); females (OR: 2.05; 95% CI: 1.39-3.04) and feeding on opossum (OR: 3.15; 95% CI: 1.85-11.69) and human blood (OR: 1.55; 95% CI: 1.07-2.24). Regarding the DTUs, we observed TcI (67.3%), TcII (6.7%), TcIII (8.7%), TcIV (4.0%) and TcV (6.0%). Across the samples typed as TcI, we detected TcIDom (19%) and sylvatic TcI (75%). The frequencies of feeding sources were 59.4% (human blood); 11.2% (hen); 9.6% (bat); 5.6% (opossum); 5.1% (mouse); 4.1% (dog); 3.0% (rodent); 1.0% (armadillo); and 1.0% (cow). Conclusions: New scenarios of T. cruzi transmission caused by secondary and sylvatic vectors are considered. The findings of sylvatic DTUs from bugs collected in domestic and peridomestic ecotopes confirms the emerging transmission scenarios in Colombia. © 2016 The Author(s).
publishDate 2016
dc.date.issued.none.fl_str_mv 2016
dc.date.accessioned.none.fl_str_mv 2021-12-16T22:15:50Z
dc.date.available.none.fl_str_mv 2021-12-16T22:15:50Z
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
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dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.none.fl_str_mv https://doi.org/10.17013/risti.19.15-31
https://revistas.udistrital.edu.co/index.php/cpaz/article/view/11695/12853
dc.identifier.issn.spa.fl_str_mv 17563305
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/41844
dc.identifier.bibliographicCitation.spa.fl_str_mv Hernández C,Salazar C,Brochero H,Teherán A,Buitrago LS,Vera M,Soto H,Florez Z,Ardila S,Parra G,Ramírez JD. Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units. Parasit Vectors. 2016. 9. (1):p. 620-620. .
url https://doi.org/10.17013/risti.19.15-31
https://revistas.udistrital.edu.co/index.php/cpaz/article/view/11695/12853
https://hdl.handle.net/20.500.12494/41844
identifier_str_mv 17563305
Hernández C,Salazar C,Brochero H,Teherán A,Buitrago LS,Vera M,Soto H,Florez Z,Ardila S,Parra G,Ramírez JD. Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units. Parasit Vectors. 2016. 9. (1):p. 620-620. .
dc.relation.ispartofjournal.spa.fl_str_mv PARASITE VECTOR
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/closedAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_14cb
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dc.format.extent.spa.fl_str_mv 620-620
dc.publisher.spa.fl_str_mv BioMed Central Ltd.
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
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spelling Hernández C.Salazar C.Brochero H.Teherán A.Buitrago L.S.Vera M.Soto H.Florez-Rivadeneira Z.Ardila S.Parra Henao, Gabriel JaimeRamírez J.D.2021-12-16T22:15:50Z2021-12-16T22:15:50Z2016https://doi.org/10.17013/risti.19.15-31https://revistas.udistrital.edu.co/index.php/cpaz/article/view/11695/1285317563305https://hdl.handle.net/20.500.12494/41844Hernández C,Salazar C,Brochero H,Teherán A,Buitrago LS,Vera M,Soto H,Florez Z,Ardila S,Parra G,Ramírez JD. Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units. Parasit Vectors. 2016. 9. (1):p. 620-620. .Background: Trypanosoma cruzi is the causative agent of Chagas disease. Due to its genetic diversity has been classified into six Discrete Typing Units (DTUs) in association with transmission cycles. In Colombia, natural T. cruzi infection has been detected in 15 triatomine species. There is scarce information regarding the infection rates, DTUs and feeding preferences of secondary vectors. Therefore, the aim of this study was to determine T. cruzi infection rates, parasite DTU, ecotopes, insect stages, geographical location and bug feeding preferences across six different triatomine species. Methods: A total of 245 insects were collected in seven departments of Colombia. We conducted molecular detection and genotyping of T. cruzi with subsequent identification of food sources. The frequency of infection, DTUs, TcI genotypes and feeding sources were plotted across the six species studied. A logistic regression model risk was estimated with insects positive for T. cruzi according to demographic and eco-epidemiological characteristics. Results: We collected 85 specimens of Panstrongylus geniculatus, 77 Rhodnius prolixus, 37 R. pallescens, 34 Triatoma maculata, 8 R. pictipes and 4 T. dimidiata. The overall T. cruzi infection rate was 61.2% and presented statistical associations with the departments Meta (OR: 2.65; 95% CI: 1.69-4.17) and Guajira (OR: 2.13; 95% CI: 1.16-3.94); peridomestic ecotope (OR: 2.52: 95% CI: 1.62-3.93); the vector species P. geniculatus (OR: 2.40; 95% CI: 1.51-3.82) and T. maculata (OR: 2.09; 95% CI: 1.02-4.29); females (OR: 2.05; 95% CI: 1.39-3.04) and feeding on opossum (OR: 3.15; 95% CI: 1.85-11.69) and human blood (OR: 1.55; 95% CI: 1.07-2.24). Regarding the DTUs, we observed TcI (67.3%), TcII (6.7%), TcIII (8.7%), TcIV (4.0%) and TcV (6.0%). Across the samples typed as TcI, we detected TcIDom (19%) and sylvatic TcI (75%). The frequencies of feeding sources were 59.4% (human blood); 11.2% (hen); 9.6% (bat); 5.6% (opossum); 5.1% (mouse); 4.1% (dog); 3.0% (rodent); 1.0% (armadillo); and 1.0% (cow). Conclusions: New scenarios of T. cruzi transmission caused by secondary and sylvatic vectors are considered. The findings of sylvatic DTUs from bugs collected in domestic and peridomestic ecotopes confirms the emerging transmission scenarios in Colombia. © 2016 The Author(s).0000-0003-4535-6521gabriel.parrah@ucc.edu.co620-620BioMed Central Ltd.ArticleChagas diseaseColombiacontrolled studydisease transmissionfeeding behaviorfemalegenetic variabilitygenotypehost parasite interactionhumanlimit of quantitationmalenonhumanPanstrongylusPanstrongylus geniculatusRhodniusRhodnius pallescensRhodnius pictipesRhodnius prolixusTriatomaTriatoma dimidiataTriatoma maculataTrypanosoma cruzianimalChagas diseaseclassificationdisease carrierdisease transmissiongeneticsisolation and purificationparasitologyprevalencetransmissionTriatominaeAnimalsChagas DiseaseColombiaDisease TransmissionInfectiousDisease VectorsGenotypeHumansPrevalenceTriatominaeTrypanosoma cruziUntangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing unitsArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionPARASITE VECTORinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/41844oai:repository.ucc.edu.co:20.500.12494/418442024-08-20 16:19:06.06metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com