Precursor forms of vitamin D reduce HIV-1 infection in vitro
Artículo Científico
- Autores:
-
Aguilar Jiménez, Wbeimar
Villegas Ospina, Simón
Gonzalez, Sandra
Zapata Builes, Wildeman
Saulle, Irma
Garziano, Micaela
Biasin, Mara
Clerici, Mario
Rugeles López, María Teresa
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2016
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/1327
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/1327
- Palabra clave:
- HIV-1
Vitamin D
Immune activation
Infection susceptibility
- Rights
- embargoedAccess
- License
- Licencia CC
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| dc.title.spa.fl_str_mv |
Precursor forms of vitamin D reduce HIV-1 infection in vitro |
| title |
Precursor forms of vitamin D reduce HIV-1 infection in vitro |
| spellingShingle |
Precursor forms of vitamin D reduce HIV-1 infection in vitro HIV-1 Vitamin D Immune activation Infection susceptibility |
| title_short |
Precursor forms of vitamin D reduce HIV-1 infection in vitro |
| title_full |
Precursor forms of vitamin D reduce HIV-1 infection in vitro |
| title_fullStr |
Precursor forms of vitamin D reduce HIV-1 infection in vitro |
| title_full_unstemmed |
Precursor forms of vitamin D reduce HIV-1 infection in vitro |
| title_sort |
Precursor forms of vitamin D reduce HIV-1 infection in vitro |
| dc.creator.fl_str_mv |
Aguilar Jiménez, Wbeimar Villegas Ospina, Simón Gonzalez, Sandra Zapata Builes, Wildeman Saulle, Irma Garziano, Micaela Biasin, Mara Clerici, Mario Rugeles López, María Teresa |
| dc.contributor.author.none.fl_str_mv |
Aguilar Jiménez, Wbeimar Villegas Ospina, Simón Gonzalez, Sandra Zapata Builes, Wildeman Saulle, Irma Garziano, Micaela Biasin, Mara Clerici, Mario Rugeles López, María Teresa |
| dc.subject.spa.fl_str_mv |
HIV-1 Vitamin D Immune activation Infection susceptibility |
| topic |
HIV-1 Vitamin D Immune activation Infection susceptibility |
| description |
Artículo Científico |
| publishDate |
2016 |
| dc.date.issued.none.fl_str_mv |
2016-12-15 |
| dc.date.accessioned.none.fl_str_mv |
2017-08-23T22:33:35Z |
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2017-08-23T22:33:35Z 2018-01 |
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Artículo |
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Aguilar Jimenez, W., Villegas Ospina, S., Gonzalez, S., Zapata, W., Saulle, I., Garziano, M., … Rugeles, M. T. (2016). Precursor Forms of Vitamin D Reduce HIV-1 Infection In Vitro. JAIDS Journal of Acquired Immune Deficiency Syndromes, 73(5). Retrieved from http://journals.lww.com/jaids/Fulltext/2016/12150/Precursor_Forms_of_Vitamin_D_Reduce_HIV_1.2.aspx |
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Aguilar Jimenez, W., Villegas Ospina, S., Gonzalez, S., Zapata, W., Saulle, I., Garziano, M., … Rugeles, M. T. (2016). Precursor Forms of Vitamin D Reduce HIV-1 Infection In Vitro. JAIDS Journal of Acquired Immune Deficiency Syndromes, 73(5). Retrieved from http://journals.lww.com/jaids/Fulltext/2016/12150/Precursor_Forms_of_Vitamin_D_Reduce_HIV_1.2.aspx |
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https://insights.ovid.com/pubmed?pmid=27509245 |
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http://journals.lww.com/jaids/Abstract/2016/12150/Precursor_Forms_of_Vitamin_D_Reduce_HIV_1.2.aspx |
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Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Medellín y Envigado, Colombia, 00000 |
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Medicina |
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Medellín |
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Universidad Cooperativa de Colombia |
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Aguilar Jiménez, WbeimarVillegas Ospina, SimónGonzalez, SandraZapata Builes, WildemanSaulle, IrmaGarziano, MicaelaBiasin, MaraClerici, MarioRugeles López, María Teresa2017-08-23T22:33:35Z2018-012017-08-23T22:33:35Z2016-12-15https://hdl.handle.net/20.500.12494/1327Aguilar Jimenez, W., Villegas Ospina, S., Gonzalez, S., Zapata, W., Saulle, I., Garziano, M., … Rugeles, M. T. (2016). Precursor Forms of Vitamin D Reduce HIV-1 Infection In Vitro. JAIDS Journal of Acquired Immune Deficiency Syndromes, 73(5). Retrieved from http://journals.lww.com/jaids/Fulltext/2016/12150/Precursor_Forms_of_Vitamin_D_Reduce_HIV_1.2.aspxArtículo CientíficoBackground: Although the anti-HIV-1 effects of vitamin D (VitD) have been reported, mechanisms behind such protection remain largely unexplored. Methods: The effects of two precursor forms (cholecalciferol/calciol at 0.01, 1 and 100 nM and calcidiol at 100 and 250 nM) on HIV-1 infection, immune activation, and gene expression were analyzed in vitro in cells of Colombian and Italian healthy donors. We quantified levels of released p24 by enzyme-linked immunosorbent assay, of intracellular p24 and cell-surface expression of CD38 and HLA-DR by flow cytometry, and mRNA expression of antiviral and immunoregulatory genes by real-time reverse transcriptionpolymerase chain reaction. Results: Cholecalciferol decreased the frequency of HIV-1-infected p24+CD4+ T cells and levels of p24 in supernatants in a dosedependent manner. Moreover, the CD4+CD38+HLA-DR+ and CD4+CD382HLA-DR+ subpopulations were more susceptible to infection but displayed the greatest cholecalciferol-induced decreases in infection rate by an X4-tropic strain. Likewise, cholecalciferol at its highest concentration decreased the frequency of CD382HLA-DR+ but not of CD38+HLA-DR+ T-cell subsets. Analyzing the effects of calcidiol, the main VitD source for immune cells and an R5-tropic strain as the most frequently transmitted virus, a reduction in HIV-1 productive infection was also observed. In addition, an increase in mRNA expression of APOBEC3G and PI3 and a reduction of TRIM22 and CCR5 expression, this latter positively correlated with p24 levels, was noted. Conclusions: VitD reduces HIV-1 infection in T cells possibly by inducing antiviral gene expression, reducing the viral co-receptor CCR5 and, at least at the highest holecalciferol concentration, by promoting an HIV-1-restrictive CD38+HLA-DR2 immunophenotype.wildeman.zapatab@campusucc.edu.coUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Medellín y Envigado, Colombia, 00000MedicinaMedellínhttps://insights.ovid.com/pubmed?pmid=27509245http://journals.lww.com/jaids/Abstract/2016/12150/Precursor_Forms_of_Vitamin_D_Reduce_HIV_1.2.aspxHIV-1Vitamin DImmune activationInfection susceptibilityPrecursor forms of vitamin D reduce HIV-1 infection in vitroArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionLicencia 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