Development of an optimized method for processing peripheral blood mononuclear cells for 1H-nuclear magnetic resonance-based metabolomic profiling
Human peripheral blood mononuclear cells (PBMCs) are part of the innate and adaptive immune system, and form a critical interface between both systems. Studying the metabolic profile of PBMC could provide valuable information about the response to pathogens, toxins or cancer, the detection of drug t...
- Autores:
-
Zapata Builes, Wildeman
Gómez Archila, León Gabriel
Galeano, Elkin
Palomino Schätzlein, Martina
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2021
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/33500
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/33500
- Palabra clave:
- Human peripheral blood mononuclear cells
Metabolomic profiling
Nuclear magnetic resonance
Folch Method
Human peripheral blood mononuclear cells
Metabolomic profiling
Nuclear magnetic resonance
Folch Method
- Rights
- openAccess
- License
- Atribución
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| dc.title.spa.fl_str_mv |
Development of an optimized method for processing peripheral blood mononuclear cells for 1H-nuclear magnetic resonance-based metabolomic profiling |
| title |
Development of an optimized method for processing peripheral blood mononuclear cells for 1H-nuclear magnetic resonance-based metabolomic profiling |
| spellingShingle |
Development of an optimized method for processing peripheral blood mononuclear cells for 1H-nuclear magnetic resonance-based metabolomic profiling Human peripheral blood mononuclear cells Metabolomic profiling Nuclear magnetic resonance Folch Method Human peripheral blood mononuclear cells Metabolomic profiling Nuclear magnetic resonance Folch Method |
| title_short |
Development of an optimized method for processing peripheral blood mononuclear cells for 1H-nuclear magnetic resonance-based metabolomic profiling |
| title_full |
Development of an optimized method for processing peripheral blood mononuclear cells for 1H-nuclear magnetic resonance-based metabolomic profiling |
| title_fullStr |
Development of an optimized method for processing peripheral blood mononuclear cells for 1H-nuclear magnetic resonance-based metabolomic profiling |
| title_full_unstemmed |
Development of an optimized method for processing peripheral blood mononuclear cells for 1H-nuclear magnetic resonance-based metabolomic profiling |
| title_sort |
Development of an optimized method for processing peripheral blood mononuclear cells for 1H-nuclear magnetic resonance-based metabolomic profiling |
| dc.creator.fl_str_mv |
Zapata Builes, Wildeman Gómez Archila, León Gabriel Galeano, Elkin Palomino Schätzlein, Martina |
| dc.contributor.advisor.none.fl_str_mv |
Zapata Builes, Wildeman |
| dc.contributor.author.none.fl_str_mv |
Zapata Builes, Wildeman Gómez Archila, León Gabriel Galeano, Elkin Palomino Schätzlein, Martina |
| dc.subject.spa.fl_str_mv |
Human peripheral blood mononuclear cells Metabolomic profiling Nuclear magnetic resonance Folch Method |
| topic |
Human peripheral blood mononuclear cells Metabolomic profiling Nuclear magnetic resonance Folch Method Human peripheral blood mononuclear cells Metabolomic profiling Nuclear magnetic resonance Folch Method |
| dc.subject.other.spa.fl_str_mv |
Human peripheral blood mononuclear cells Metabolomic profiling Nuclear magnetic resonance Folch Method |
| description |
Human peripheral blood mononuclear cells (PBMCs) are part of the innate and adaptive immune system, and form a critical interface between both systems. Studying the metabolic profile of PBMC could provide valuable information about the response to pathogens, toxins or cancer, the detection of drug toxicity, in drug discovery and cell replacement therapy. The primary purpose of this study was to develop an improved processing method for PBMCs metabolomic profiling with nuclear magnetic resonance (NMR) spectroscopy. To this end, an experimental design was applied to develop an alternative method to process PBMCs at low concentrations. The design included the isolation of PBMCs from the whole blood of four different volunteers, of whom 27 cell samples were processed by two different techniques for quenching and extraction of metabolites: a traditional one using organic solvents and an alternative one employing a high-intensity ultrasound probe, the latter with a variation that includes the use of deproteinizing filters. Finally, all the samples were characterized by 1H-NMR and the metabolomic profiles were compared by the method. As a result, two new methods for PBMCs processing, called Ultrasound Method (UM) and Ultrasound and Ultrafiltration Method (UUM), are described and compared to the Folch Method (FM), which is the standard protocol for extracting metabolites from cell samples. We found that UM and UUM were superior to FM in terms of sensitivity, processing time, spectrum quality, amount of identifiable, quantifiable metabolites and reproducibility. |
| publishDate |
2021 |
| dc.date.accessioned.none.fl_str_mv |
2021-03-01T16:10:41Z |
| dc.date.available.none.fl_str_mv |
2021-03-01T16:10:41Z |
| dc.date.issued.none.fl_str_mv |
2021-02-25 |
| dc.type.none.fl_str_mv |
Artículos Científicos |
| dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.coarversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.issn.spa.fl_str_mv |
1932-6203 |
| dc.identifier.uri.spa.fl_str_mv |
10.1371/journal.pone.0247668 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12494/33500 |
| dc.identifier.bibliographicCitation.spa.fl_str_mv |
Gómez-Archila, L.G., Palomino-Schätzlein, M., Zapata-Builes, W. y Galeano, E. (2021) Development of an optimized method for processing peripheral blood mononuclear cells for 1H-nuclear magnetic resonance-based metabolomic profiling. PLoS ONE 16(2): e0247668. https://doi.org/10.1371/journal.pone.0247668 |
| identifier_str_mv |
1932-6203 10.1371/journal.pone.0247668 Gómez-Archila, L.G., Palomino-Schätzlein, M., Zapata-Builes, W. y Galeano, E. (2021) Development of an optimized method for processing peripheral blood mononuclear cells for 1H-nuclear magnetic resonance-based metabolomic profiling. PLoS ONE 16(2): e0247668. https://doi.org/10.1371/journal.pone.0247668 |
| url |
https://hdl.handle.net/20.500.12494/33500 |
| dc.relation.isversionof.spa.fl_str_mv |
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0247668 |
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PLOS ONE |
| dc.relation.references.spa.fl_str_mv |
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Zapata Builes, WildemanZapata Builes, WildemanGómez Archila, León GabrielGaleano, ElkinPalomino Schätzlein, Martina16 (2)2021-03-01T16:10:41Z2021-03-01T16:10:41Z2021-02-251932-620310.1371/journal.pone.0247668https://hdl.handle.net/20.500.12494/33500Gómez-Archila, L.G., Palomino-Schätzlein, M., Zapata-Builes, W. y Galeano, E. (2021) Development of an optimized method for processing peripheral blood mononuclear cells for 1H-nuclear magnetic resonance-based metabolomic profiling. PLoS ONE 16(2): e0247668. https://doi.org/10.1371/journal.pone.0247668Human peripheral blood mononuclear cells (PBMCs) are part of the innate and adaptive immune system, and form a critical interface between both systems. Studying the metabolic profile of PBMC could provide valuable information about the response to pathogens, toxins or cancer, the detection of drug toxicity, in drug discovery and cell replacement therapy. The primary purpose of this study was to develop an improved processing method for PBMCs metabolomic profiling with nuclear magnetic resonance (NMR) spectroscopy. To this end, an experimental design was applied to develop an alternative method to process PBMCs at low concentrations. The design included the isolation of PBMCs from the whole blood of four different volunteers, of whom 27 cell samples were processed by two different techniques for quenching and extraction of metabolites: a traditional one using organic solvents and an alternative one employing a high-intensity ultrasound probe, the latter with a variation that includes the use of deproteinizing filters. Finally, all the samples were characterized by 1H-NMR and the metabolomic profiles were compared by the method. As a result, two new methods for PBMCs processing, called Ultrasound Method (UM) and Ultrasound and Ultrafiltration Method (UUM), are described and compared to the Folch Method (FM), which is the standard protocol for extracting metabolites from cell samples. We found that UM and UUM were superior to FM in terms of sensitivity, processing time, spectrum quality, amount of identifiable, quantifiable metabolites and reproducibility.Human peripheral blood mononuclear cells (PBMCs) are part of the innate and adaptive immune system, and form a critical interface between both systems. Studying the metabolic profile of PBMC could provide valuable information about the response to pathogens, toxins or cancer, the detection of drug toxicity, in drug discovery and cell replacement therapy. The primary purpose of this study was to develop an improved processing method for PBMCs metabolomic profiling with nuclear magnetic resonance (NMR) spectroscopy. To this end, an experimental design was applied to develop an alternative method to process PBMCs at low concentrations. The design included the isolation of PBMCs from the whole blood of four different volunteers, of whom 27 cell samples were processed by two different techniques for quenching and extraction of metabolites: a traditional one using organic solvents and an alternative one employing a high-intensity ultrasound probe, the latter with a variation that includes the use of deproteinizing filters. Finally, all the samples were characterized by 1H-NMR and the metabolomic profiles were compared by the method. As a result, two new methods for PBMCs processing, called Ultrasound Method (UM) and Ultrasound and Ultrafiltration Method (UUM), are described and compared to the Folch Method (FM), which is the standard protocol for extracting metabolites from cell samples. We found that UM and UUM were superior to FM in terms of sensitivity, processing time, spectrum quality, amount of identifiable, quantifiable metabolites and reproducibility.https://scienti.minciencias.gov.co/cvlac/EnProdArticulo/query.do?cod_producto=73&cod_rh=0000157775https://orcid.org/0000-0002-7351-8738COL0112548wildeman.zapatab@campusucc.edu.cohttps://scholar.google.com.co/citations?hl=en&user=VLZxl1UAAAAJ20Oscar Millet, CIC bioGUNE, SPAINUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Medellín y EnvigadoMedicinaMedellínhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0247668PLOS ONEDelves PJ, Martin SJ, Burton DR, Roitt IM. Roitt’s essential immunology. 2017.Kleiveland CR. Peripheral Blood Mononuclear Cells BT—The Impact of Food Bioactives on Health: in vitro and ex vivo models. In: Verhoeckx K, Cotter P, Lo´pez-Expo´ sito I, Kleiveland C, Lea T, Mackie A, et al., editors. 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PLoS One. 2018; 13(7):1–15. https://doi.org/10.1371/journal.pone.0199351 PMID: 30005063Human peripheral blood mononuclear cellsMetabolomic profilingNuclear magnetic resonanceFolch MethodHuman peripheral blood mononuclear cellsMetabolomic profilingNuclear magnetic resonanceFolch MethodDevelopment of an optimized method for processing peripheral blood mononuclear cells for 1H-nuclear magnetic resonance-based metabolomic profilingArtículos Científicoshttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribucióninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationORIGINALmetabolomica UCC 2021.pdfmetabolomica UCC 2021.pdfArtículoapplication/pdf2002736https://repository.ucc.edu.co/bitstreams/44684556-a980-46d5-a627-595447558bbe/download023fcebc6b894fe23330631ade9d282fMD51LICENSElicense.txtlicense.txttext/plain; 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