Genomic epidemiology of severe acute Respiratory Syndrome Coronavirus 2, Colombia
Coronavirus disease (COVID-19) in Colombia was first diagnosed in a traveler arriving from Italy on February 26, 2020. However, limited data are available on the origins and number of introductions of COVID-19 into the country. We sequenced the causative agent of COVID-19, severe acute respiratory s...
- Autores:
-
Laiton Donato, Katherine
Villabona Arenas, Christian J.
Usme Ciro, José Aldemar
Franco Muñoz, Carlos
Álvarez Díaz, Diego Alejandro
Villabona Arenas, Liz S.
Echeverria Londoño, Susy
Cucunuba, Zulma M.
Franco Sierra, Nicolas D.
Flórez Sánchez, Astrid C.
Ferro, Carolina
Ajami, Nadim J.
Walteros, Diana Marcela
Prieto, Franklin
Duran, Carlos A.
Ospina Martínez, Martha L.
Mercado Reyes, Marcela
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2020
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/32678
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/32678
- Palabra clave:
- Coronavirus
Secuenciación
Filogenómica
COVID-19
Colombia
SARS-CoV-2
Genome sequencing
Phylogenomics
COVID-19
Colombia
- Rights
- openAccess
- License
- Atribución
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Genomic epidemiology of severe acute Respiratory Syndrome Coronavirus 2, Colombia |
| title |
Genomic epidemiology of severe acute Respiratory Syndrome Coronavirus 2, Colombia |
| spellingShingle |
Genomic epidemiology of severe acute Respiratory Syndrome Coronavirus 2, Colombia Coronavirus Secuenciación Filogenómica COVID-19 Colombia SARS-CoV-2 Genome sequencing Phylogenomics COVID-19 Colombia |
| title_short |
Genomic epidemiology of severe acute Respiratory Syndrome Coronavirus 2, Colombia |
| title_full |
Genomic epidemiology of severe acute Respiratory Syndrome Coronavirus 2, Colombia |
| title_fullStr |
Genomic epidemiology of severe acute Respiratory Syndrome Coronavirus 2, Colombia |
| title_full_unstemmed |
Genomic epidemiology of severe acute Respiratory Syndrome Coronavirus 2, Colombia |
| title_sort |
Genomic epidemiology of severe acute Respiratory Syndrome Coronavirus 2, Colombia |
| dc.creator.fl_str_mv |
Laiton Donato, Katherine Villabona Arenas, Christian J. Usme Ciro, José Aldemar Franco Muñoz, Carlos Álvarez Díaz, Diego Alejandro Villabona Arenas, Liz S. Echeverria Londoño, Susy Cucunuba, Zulma M. Franco Sierra, Nicolas D. Flórez Sánchez, Astrid C. Ferro, Carolina Ajami, Nadim J. Walteros, Diana Marcela Prieto, Franklin Duran, Carlos A. Ospina Martínez, Martha L. Mercado Reyes, Marcela |
| dc.contributor.author.none.fl_str_mv |
Laiton Donato, Katherine Villabona Arenas, Christian J. Usme Ciro, José Aldemar Franco Muñoz, Carlos Álvarez Díaz, Diego Alejandro Villabona Arenas, Liz S. Echeverria Londoño, Susy Cucunuba, Zulma M. Franco Sierra, Nicolas D. Flórez Sánchez, Astrid C. Ferro, Carolina Ajami, Nadim J. Walteros, Diana Marcela Prieto, Franklin Duran, Carlos A. Ospina Martínez, Martha L. Mercado Reyes, Marcela |
| dc.subject.spa.fl_str_mv |
Coronavirus Secuenciación Filogenómica COVID-19 Colombia |
| topic |
Coronavirus Secuenciación Filogenómica COVID-19 Colombia SARS-CoV-2 Genome sequencing Phylogenomics COVID-19 Colombia |
| dc.subject.other.spa.fl_str_mv |
SARS-CoV-2 Genome sequencing Phylogenomics COVID-19 Colombia |
| description |
Coronavirus disease (COVID-19) in Colombia was first diagnosed in a traveler arriving from Italy on February 26, 2020. However, limited data are available on the origins and number of introductions of COVID-19 into the country. We sequenced the causative agent of COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), from 43 clinical samples we collected, along with another 79 genome sequences available from Colombia. We investigated the emergence and importation routes for SARSCoV-2 into Colombia by using epidemiologic, historical air travel, and phylogenetic observations. Our study provides evidence of multiple introductions, mostly from Europe, and documents >12 lineages. Phylogenetic findings validate the lineage diversity, support multiple importation events, and demonstrate the evolutionary relationship of epidemiologically linked transmission chains. Our results reconstruct the early evolutionary history of SARS-CoV-2 in Colombia and highlight the advantages of genome sequencing to complement COVID-19 outbreak investigations. |
| publishDate |
2020 |
| dc.date.issued.none.fl_str_mv |
2020-11-16 |
| dc.date.accessioned.none.fl_str_mv |
2021-01-20T19:13:54Z |
| dc.date.available.none.fl_str_mv |
2021-01-20T19:13:54Z |
| dc.type.none.fl_str_mv |
Artículo |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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1080-6059 |
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10.3201/eid2612.202969 |
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https://hdl.handle.net/20.500.12494/32678 |
| dc.identifier.bibliographicCitation.spa.fl_str_mv |
Laiton-Donato, K., Villabona-Arenas, C., Usme-Ciro, J. A., Franco-Muñoz, C., Álvarez-Díaz, D. A., Villabona-Arenas, L....Mercado-Reyes, M. (2020). Genomic Epidemiology of Severe Acute Respiratory Syndrome Coronavirus 2, Colombia. Emerging Infectious Diseases, 26(12), 2854-2862. https://dx.doi.org/10.3201/eid2612.202969. |
| identifier_str_mv |
1080-6059 10.3201/eid2612.202969 Laiton-Donato, K., Villabona-Arenas, C., Usme-Ciro, J. A., Franco-Muñoz, C., Álvarez-Díaz, D. A., Villabona-Arenas, L....Mercado-Reyes, M. (2020). Genomic Epidemiology of Severe Acute Respiratory Syndrome Coronavirus 2, Colombia. Emerging Infectious Diseases, 26(12), 2854-2862. https://dx.doi.org/10.3201/eid2612.202969. |
| url |
https://hdl.handle.net/20.500.12494/32678 |
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https://wwwnc.cdc.gov/eid/article/26/12/20-2969_article |
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Emerging Infectious Diseases |
| dc.relation.references.spa.fl_str_mv |
Wu F, Zhao S, Yu B, Chen Y-M, Wang W, Song Z-G, et al. A new coronavirus associated with human respiratory disease in China. Nature. 2020;579:265–9. https://doi.org/10.1038/ s41586-020-2008-3 World Health Organization. Novel coronavirus—China, 2020 Jan 12 [cited 2020 Jun 16]. https://www.who.int/csr/ don/12-january-2020-novel-coronavirus-china Kraemer MUG, Yang C-H, Gutierrez B, Wu C-H, Klein B, Pigott DM, et al.; Open COVID-19 Data Working Group. The effect of human mobility and control measures on the COVID-19 epidemic in China. Science. 2020;368:493–7. https://doi.org/10.1126/science.abb4218 He X, Lau EHY, Wu P, Deng X, Wang J, Hao X, et al. Temporal dynamics in viral shedding and transmissibility of COVID-19. Nat Med. 2020;26:672–5. https://doi.org/ 10.1038/s41591-020-0869-5 Li J, Zhang L, Liu B, Song D. Case report: viral shedding for 60 days in a woman with COVID-19. Am J Trop Med Hyg. 2020;102:1210–3. https://doi.org/10.4269/ajtmh.20-0275 Jarvis CI, Van Zandvoort K, Gimma A, Prem K, Klepac P, Rubin GJ, et al. CMMID COVID-19 working group. Quantifying the impact of physical distance measures on the transmission of COVID-19 in the UK. BMC Med. 2020;18:124. https://doi.org/10.1186/s12916-020-01597-8 Prem K, Liu Y, Russell TW, Kucharski AJ, Eggo RM, Davies N, et al.; Centre for the Mathematical Modelling of Infectious Diseases COVID-19 Working Group. The effect of control strategies to reduce social mixing on outcomes of the COVID-19 epidemic in Wuhan, China: a modelling study. Lancet Public Health. 2020;5:e261–70. https://doi.org/ 10.1016/S2468-2667(20)30073-6 Hellewell J, Abbott S, Gimma A, Bosse NI, Jarvis CI, Russell TW, et al.; Centre for the Mathematical Modelling of Infectious Diseases COVID-19 Working Group. Feasibility of controlling COVID-19 outbreaks by isolation of cases and contacts. Lancet Glob Health. 2020;8:e488–96. https://doi.org/ 10.1016/S2214-109X(20)30074-7 Chinazzi M, Davis JT, Ajelli M, Gioannini C, Litvinova M, Merler S, et al. The effect of travel restrictions on the spread of the 2019 novel coronavirus (COVID-19) outbreak. Science. 2020;368:395–400. https://doi.org/10.1126/ science.aba9757 Ribeiro F, Leist A. Who is going to pay the price of Covid-19? Reflections about an unequal Brazil. Int J Equity Health. 2020;19:91. https://doi.org/10.1186/s12939-020-01207-2 Ministry of Health and Social Protection. Colombia. Minsalud confirms six new cases of coronavirus (COVID-19) in Colombia [in Spanish]. Bogotá, Colombia; Boletín de Prensa no. 057 de 2020. 2020 [cited 2020 May 24]. https://www.minsalud.gov.co/Paginas/Minsaludconfirma-seis-nuevos-casos-de-coronavirus-(COVID-19)- en-Colombia.aspx National Institute of Health. Colombia. COVID-2019 in Colombia daily report 2020 March 31 [in Spanish] [cited 2020 Jun 2]. https://www.ins.gov.co/Noticias/Paginas/ Coronavirus.aspx National Institute of Health. Colombia. COVID-2019 in Colombia daily report 2020 Jun 18 [in Spanish] [cited 2020 Jun 2]. https://www.ins.gov.co/Noticias/Paginas/ Coronavirus.aspx Grubaugh ND, Ladner JT, Lemey P, Pybus OG, Rambaut A, Holmes EC, et al. Tracking virus outbreaks in the twentyfirst century. Nat Microbiol. 2019;4:10–9. https://doi.org/ 10.1038/s41564-018-0296-2 Lu J, du Plessis L, Liu Z, Hill V, Kang M, Lin H, et al. Genomic epidemiology of SARS-CoV-2 in Guangdong Province, China. Cell. 2020;181:997–1003.e9. https://doi.org/ 10.1016/j.cell.2020.04.023 Eden J-S, Rockett R, Carter I, Rahman H, de Ligt J, Hadfield J, et al. An emergent clade of SARS-CoV-2 linked to returned travellers from Iran. Virus Evol. 2020;6:veaa027. https://doi.org/10.1093/ve/veaa027 Gudbjartsson DF, Helgason A, Jonsson H, Magnusson OT, Melsted P, Norddahl GL, et al. Spread of SARS-CoV-2 in the Icelandic Population. N Engl J Med. 2020;382:2302–15. https://doi.org/10.1056/NEJMoa2006100 Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25:2000045. https://doi.org/10.2807/1560-7917. ES.2020.25.3.2000045 Quick J. nCoV-2019 sequencing protocol version 1. protocols. io; 2020 Jan 22 [cited 2020 Mar 2]. https://www.protocols. io/view/ncov-2019-sequencing-protocol-bbmuik6w Wick R. Porechop version 0.2.4. 2018 Oct 19 [cited 2020 Jun 18]. https://github.com/rrwick/Porechop Li H, Durbin R. Fast and accurate long-read alignment with Burrows-Wheeler transform. Bioinformatics. 2010;26:589–95. https://doi.org/10.1093/bioinformatics/btp698 Simpson J. Nanopolish: signal-level algorithms for MinION data [cited 2020 May 10]. https://github com/jts/nanopolish Chen S, Zhou Y, Chen Y, Gu J. fastp: an ultra-fast all-inone FASTQ preprocessor. Bioinformatics. 2018;34:i884–90. https://doi.org/10.1093/bioinformatics/bty560 Bushnell B. BBMap short read aligner, and other bioinformatic tools [cited 2020 May 10]. https://escholarship.org/uc/ item/1h3515gn Hadfield J, Megill C, Bell SM, Huddleston J, Potter B, Callender C, et al. Nextstrain: real-time tracking of pathogen evolution. Bioinformatics. 2018;34:4121–3. https://doi.org/10.1093/bioinformatics/bty407 Rambaut A, Holmes EC, Hill V, O’Toole Á, McCrone JT, Ruis C, et al. A dynamic nomenclature proposal for SARSCoV-2 to assist genomic epidemiology. Nat Microbiol. 2020 Jul 15 [Epub ahead of print]. https://doi.org/10.1038/ s41564-020-0770-5 O’Toole A, McCrone JT. Pangolin: Phylogenetic Assignment of Named Global Outbreak LINeages [cited 2020 Jun 18]. https://github.com/hCoV-2019/pangolin Katoh K, Misawa K, Kuma K, Miyata T. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res. 2002;30:3059–66. https://doi.org/10.1093/nar/gkf436 De Maio N, Walker C, Borges R, Weilguny L, Slodkowicz G, Goldman N. Issues with SARS-CoV-2 sequencing data. 2020 Jul 29 [cited 2020 Jun 16]. https://virological.org/t/issueswith-sars-cov-2-sequencing-data/473 Hasegawa M, Kishino H, Yano T. Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol. 1985;22:160–74. https://doi.org/10.1007/BF02101694 Minh BQ, Schmidt HA, Chernomor O, Schrempf D, Woodhams MD, von Haeseler A, et al. IQ-TREE 2: new models and efficient methods for phylogenetic inference in the genomic era. Mol Biol Evol. 2020;37:1530–4. https://doi.org/10.1093/ molbev/msaa015 Guindon S, Dufayard J-F, Lefort V, Anisimova M, Hordijk W, Gascuel O. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol. 2010;59:307–21. https://doi.org/10.1093/sysbio/syq010 Sagulenko P, Puller V, Neher RA. TreeTime: maximumlikelihood phylodynamic analysis. Virus Evol. 2018;4:vex042. https://doi.org/10.1093/ve/vex042 To T-H, Jung M, Lycett S, Gascuel O. Fast dating using leastsquares criteria and algorithms. Syst Biol. 2016;65:82–97. https://doi.org/10.1093/sysbio/syv068 World Health Organization. Novel coronavirus (2019-nCoV) situation report—57. 2020 March 17 [cited 2020 May 10]. https://www.who.int/emergencies/diseases/novelcoronavirus-2019/situation-reports United Nations Population Division. World population prospects 2019 [cited 2020 Jun 19]. https://population. un.org/wpp/Download/Metadata/Documentation Candido DDS, Watts A, Abade L, Kraemer MUG, Pybus OG, Croda J, et al. Routes for COVID-19 importation in Brazil. J Travel Med. 2020;27:taaa042. https://doi.org/10.1093/jtm/ taaa042 Ministry of Health and Social Protection, Colombia. Resolution number 0000380 [in Spanish]. 2020 Mar 10 [cited 2020 June 2] https://www.minsalud.gov.co/ Normatividad_Nuevo/Resoluci%C3%B3n%20No.%20 380%20de%202020.pdf Ministry of Health and Social Protection, Colombia. Resolution number 0000385 [in Spanish]. 2020 Mar 12 [cited 2020 June 2] https://www.minsalud.gov.co/sites/rid/Lists/Biblioteca Digital/RIDE/DE/DIJ/resolucion-385-de-2020.pdf Ministry of the Interior, Colombia. Decree 412 [in Spanish]. 2020 Mar 16 [cited 2020 June 2]. https://dapre.presidencia. gov.co/normativa/normativa/DECRETO%20412%20 DEL%2016%20DE%20MARZO%20DE%202020.pdf Ministry of the Interior, Colombia. Decree 457 [in Spanish]. 2020 Mar 22 [cited 2020 June 2] https://dapre.presidencia. gov.co/normativa/normativa/DECRETO%20457%20 DEL%2022%20DE%20MARZO%20DE%202020.pdf Ministry of Transport, Colombia. Decree 439 [in Spanish]. 2020 Mar 20 [cited 2020 May 10] https://dapre.presidencia. gov.co/normativa/normativa/DECRETO%20439%20 DEL%2020%20DE%20MARZO%20DE%202020.pdf Chancellery of Colombia. Communication on humanitarian flights from 2 to 12 July; 2020 June 17 [cited 2020 Jun 19]. https://www.cancilleria.gov.co/newsroom/publiques/ comunicado-vuelos-caracter-humanitario-2-12-julio Sun K, Chen J, Viboud C. Early epidemiological analysis of the coronavirus disease 2019 outbreak based on crowdsourced data: a population-level observational study. Lancet Digit Health. 2020;2:e201–8. https://doi.org/10.1016/ s2589-7500(20)30026-1 Castillo AE, Parra B, Tapia P, Acevedo A, Lagos J, Andrade W, et al. Phylogenetic analysis of the first four SARS-CoV-2 cases in Chile. J Med Virol. 2020;92:1562–6. https://doi.org/10.1002/jmv.25797 Candido DS, Claro IM, de Jesus JG, Souza WM, Moreira FRR, Dellicour S, et al.; Brazil-UK Centre for Arbovirus Discovery, Diagnosis, Genomics and Epidemiology (CADDE) Genomic Network. Evolution and epidemic spread of SARS-CoV-2 in Brazil. Science. 2020;369:1255–60. https://doi.org/10.1126/ science.abd2161 Korber B, Fischer WM, Gnanakaran S, Yoon H, Theiler J, Abfalterer W, et al.; Sheffield COVID-19 Genomics Group. Tracking changes in SARS-CoV-2 spike: evidence that D614G increases infectivity of the COVID-19 virus. Cell. 2020;182:812–827.e19. https://doi.org/10.1016/ j.cell.2020.06.043 da Silva Candido D, Watts A, Abade L, Kraemer MUG, Pybus OG, Croda J, et al. Routes for COVID-19 importation in Brazil. J Travel Med. 2020;27:taaa042. https://doi.org/ 10.1093/jtm/taaa042 |
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Laiton Donato, KatherineVillabona Arenas, Christian J.Usme Ciro, José AldemarFranco Muñoz, CarlosÁlvarez Díaz, Diego AlejandroVillabona Arenas, Liz S.Echeverria Londoño, SusyCucunuba, Zulma M.Franco Sierra, Nicolas D.Flórez Sánchez, Astrid C.Ferro, CarolinaAjami, Nadim J.Walteros, Diana MarcelaPrieto, FranklinDuran, Carlos A.Ospina Martínez, Martha L.Mercado Reyes, Marcela262021-01-20T19:13:54Z2021-01-20T19:13:54Z2020-11-161080-605910.3201/eid2612.202969https://hdl.handle.net/20.500.12494/32678Laiton-Donato, K., Villabona-Arenas, C., Usme-Ciro, J. A., Franco-Muñoz, C., Álvarez-Díaz, D. A., Villabona-Arenas, L....Mercado-Reyes, M. (2020). Genomic Epidemiology of Severe Acute Respiratory Syndrome Coronavirus 2, Colombia. Emerging Infectious Diseases, 26(12), 2854-2862. https://dx.doi.org/10.3201/eid2612.202969.Coronavirus disease (COVID-19) in Colombia was first diagnosed in a traveler arriving from Italy on February 26, 2020. However, limited data are available on the origins and number of introductions of COVID-19 into the country. We sequenced the causative agent of COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), from 43 clinical samples we collected, along with another 79 genome sequences available from Colombia. We investigated the emergence and importation routes for SARSCoV-2 into Colombia by using epidemiologic, historical air travel, and phylogenetic observations. Our study provides evidence of multiple introductions, mostly from Europe, and documents >12 lineages. Phylogenetic findings validate the lineage diversity, support multiple importation events, and demonstrate the evolutionary relationship of epidemiologically linked transmission chains. Our results reconstruct the early evolutionary history of SARS-CoV-2 in Colombia and highlight the advantages of genome sequencing to complement COVID-19 outbreak investigations.https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000318507https://orcid.org/0000-0002-8093-0544https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000008981jose.usmec@campusucc.edu.cokdlaitond@unal.edu.cohttps://scholar.google.com.co/citations?user=cU2KyT4AAAAJ&hl=en2854-2862Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa MartaDr. Peter DrotmanMedicinaSanta Martahttps://wwwnc.cdc.gov/eid/article/26/12/20-2969_articleEmerging Infectious DiseasesWu F, Zhao S, Yu B, Chen Y-M, Wang W, Song Z-G, et al. A new coronavirus associated with human respiratory disease in China. Nature. 2020;579:265–9. https://doi.org/10.1038/ s41586-020-2008-3World Health Organization. Novel coronavirus—China, 2020 Jan 12 [cited 2020 Jun 16]. https://www.who.int/csr/ don/12-january-2020-novel-coronavirus-chinaKraemer MUG, Yang C-H, Gutierrez B, Wu C-H, Klein B, Pigott DM, et al.; Open COVID-19 Data Working Group. The effect of human mobility and control measures on the COVID-19 epidemic in China. Science. 2020;368:493–7. https://doi.org/10.1126/science.abb4218He X, Lau EHY, Wu P, Deng X, Wang J, Hao X, et al. Temporal dynamics in viral shedding and transmissibility of COVID-19. Nat Med. 2020;26:672–5. https://doi.org/ 10.1038/s41591-020-0869-5Li J, Zhang L, Liu B, Song D. Case report: viral shedding for 60 days in a woman with COVID-19. Am J Trop Med Hyg. 2020;102:1210–3. https://doi.org/10.4269/ajtmh.20-0275Jarvis CI, Van Zandvoort K, Gimma A, Prem K, Klepac P, Rubin GJ, et al. CMMID COVID-19 working group. Quantifying the impact of physical distance measures on the transmission of COVID-19 in the UK. BMC Med. 2020;18:124. https://doi.org/10.1186/s12916-020-01597-8Prem K, Liu Y, Russell TW, Kucharski AJ, Eggo RM, Davies N, et al.; Centre for the Mathematical Modelling of Infectious Diseases COVID-19 Working Group. The effect of control strategies to reduce social mixing on outcomes of the COVID-19 epidemic in Wuhan, China: a modelling study. Lancet Public Health. 2020;5:e261–70. https://doi.org/ 10.1016/S2468-2667(20)30073-6Hellewell J, Abbott S, Gimma A, Bosse NI, Jarvis CI, Russell TW, et al.; Centre for the Mathematical Modelling of Infectious Diseases COVID-19 Working Group. Feasibility of controlling COVID-19 outbreaks by isolation of cases and contacts. Lancet Glob Health. 2020;8:e488–96. https://doi.org/ 10.1016/S2214-109X(20)30074-7Chinazzi M, Davis JT, Ajelli M, Gioannini C, Litvinova M, Merler S, et al. The effect of travel restrictions on the spread of the 2019 novel coronavirus (COVID-19) outbreak. Science. 2020;368:395–400. https://doi.org/10.1126/ science.aba9757Ribeiro F, Leist A. Who is going to pay the price of Covid-19? Reflections about an unequal Brazil. Int J Equity Health. 2020;19:91. https://doi.org/10.1186/s12939-020-01207-2Ministry of Health and Social Protection. Colombia. Minsalud confirms six new cases of coronavirus (COVID-19) in Colombia [in Spanish]. Bogotá, Colombia; Boletín de Prensa no. 057 de 2020. 2020 [cited 2020 May 24]. https://www.minsalud.gov.co/Paginas/Minsaludconfirma-seis-nuevos-casos-de-coronavirus-(COVID-19)- en-Colombia.aspxNational Institute of Health. Colombia. COVID-2019 in Colombia daily report 2020 March 31 [in Spanish] [cited 2020 Jun 2]. https://www.ins.gov.co/Noticias/Paginas/ Coronavirus.aspxNational Institute of Health. Colombia. COVID-2019 in Colombia daily report 2020 Jun 18 [in Spanish] [cited 2020 Jun 2]. https://www.ins.gov.co/Noticias/Paginas/ Coronavirus.aspxGrubaugh ND, Ladner JT, Lemey P, Pybus OG, Rambaut A, Holmes EC, et al. Tracking virus outbreaks in the twentyfirst century. Nat Microbiol. 2019;4:10–9. https://doi.org/ 10.1038/s41564-018-0296-2Lu J, du Plessis L, Liu Z, Hill V, Kang M, Lin H, et al. Genomic epidemiology of SARS-CoV-2 in Guangdong Province, China. Cell. 2020;181:997–1003.e9. https://doi.org/ 10.1016/j.cell.2020.04.023Eden J-S, Rockett R, Carter I, Rahman H, de Ligt J, Hadfield J, et al. An emergent clade of SARS-CoV-2 linked to returned travellers from Iran. Virus Evol. 2020;6:veaa027. https://doi.org/10.1093/ve/veaa027Gudbjartsson DF, Helgason A, Jonsson H, Magnusson OT, Melsted P, Norddahl GL, et al. Spread of SARS-CoV-2 in the Icelandic Population. N Engl J Med. 2020;382:2302–15. https://doi.org/10.1056/NEJMoa2006100Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25:2000045. https://doi.org/10.2807/1560-7917. ES.2020.25.3.2000045Quick J. nCoV-2019 sequencing protocol version 1. protocols. io; 2020 Jan 22 [cited 2020 Mar 2]. https://www.protocols. io/view/ncov-2019-sequencing-protocol-bbmuik6wWick R. Porechop version 0.2.4. 2018 Oct 19 [cited 2020 Jun 18]. https://github.com/rrwick/PorechopLi H, Durbin R. Fast and accurate long-read alignment with Burrows-Wheeler transform. Bioinformatics. 2010;26:589–95. https://doi.org/10.1093/bioinformatics/btp698Simpson J. Nanopolish: signal-level algorithms for MinION data [cited 2020 May 10]. https://github com/jts/nanopolishChen S, Zhou Y, Chen Y, Gu J. fastp: an ultra-fast all-inone FASTQ preprocessor. Bioinformatics. 2018;34:i884–90. https://doi.org/10.1093/bioinformatics/bty560Bushnell B. BBMap short read aligner, and other bioinformatic tools [cited 2020 May 10]. https://escholarship.org/uc/ item/1h3515gnHadfield J, Megill C, Bell SM, Huddleston J, Potter B, Callender C, et al. Nextstrain: real-time tracking of pathogen evolution. Bioinformatics. 2018;34:4121–3. https://doi.org/10.1093/bioinformatics/bty407Rambaut A, Holmes EC, Hill V, O’Toole Á, McCrone JT, Ruis C, et al. A dynamic nomenclature proposal for SARSCoV-2 to assist genomic epidemiology. Nat Microbiol. 2020 Jul 15 [Epub ahead of print]. https://doi.org/10.1038/ s41564-020-0770-5O’Toole A, McCrone JT. Pangolin: Phylogenetic Assignment of Named Global Outbreak LINeages [cited 2020 Jun 18]. https://github.com/hCoV-2019/pangolinKatoh K, Misawa K, Kuma K, Miyata T. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res. 2002;30:3059–66. https://doi.org/10.1093/nar/gkf436De Maio N, Walker C, Borges R, Weilguny L, Slodkowicz G, Goldman N. Issues with SARS-CoV-2 sequencing data. 2020 Jul 29 [cited 2020 Jun 16]. https://virological.org/t/issueswith-sars-cov-2-sequencing-data/473Hasegawa M, Kishino H, Yano T. Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol. 1985;22:160–74. https://doi.org/10.1007/BF02101694Minh BQ, Schmidt HA, Chernomor O, Schrempf D, Woodhams MD, von Haeseler A, et al. IQ-TREE 2: new models and efficient methods for phylogenetic inference in the genomic era. Mol Biol Evol. 2020;37:1530–4. https://doi.org/10.1093/ molbev/msaa015Guindon S, Dufayard J-F, Lefort V, Anisimova M, Hordijk W, Gascuel O. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol. 2010;59:307–21. https://doi.org/10.1093/sysbio/syq010Sagulenko P, Puller V, Neher RA. TreeTime: maximumlikelihood phylodynamic analysis. Virus Evol. 2018;4:vex042. https://doi.org/10.1093/ve/vex042To T-H, Jung M, Lycett S, Gascuel O. Fast dating using leastsquares criteria and algorithms. Syst Biol. 2016;65:82–97. https://doi.org/10.1093/sysbio/syv068World Health Organization. Novel coronavirus (2019-nCoV) situation report—57. 2020 March 17 [cited 2020 May 10]. https://www.who.int/emergencies/diseases/novelcoronavirus-2019/situation-reportsUnited Nations Population Division. World population prospects 2019 [cited 2020 Jun 19]. https://population. un.org/wpp/Download/Metadata/DocumentationCandido DDS, Watts A, Abade L, Kraemer MUG, Pybus OG, Croda J, et al. Routes for COVID-19 importation in Brazil. J Travel Med. 2020;27:taaa042. https://doi.org/10.1093/jtm/ taaa042Ministry of Health and Social Protection, Colombia. Resolution number 0000380 [in Spanish]. 2020 Mar 10 [cited 2020 June 2] https://www.minsalud.gov.co/ Normatividad_Nuevo/Resoluci%C3%B3n%20No.%20 380%20de%202020.pdfMinistry of Health and Social Protection, Colombia. Resolution number 0000385 [in Spanish]. 2020 Mar 12 [cited 2020 June 2] https://www.minsalud.gov.co/sites/rid/Lists/Biblioteca Digital/RIDE/DE/DIJ/resolucion-385-de-2020.pdfMinistry of the Interior, Colombia. Decree 412 [in Spanish]. 2020 Mar 16 [cited 2020 June 2]. https://dapre.presidencia. gov.co/normativa/normativa/DECRETO%20412%20 DEL%2016%20DE%20MARZO%20DE%202020.pdfMinistry of the Interior, Colombia. Decree 457 [in Spanish]. 2020 Mar 22 [cited 2020 June 2] https://dapre.presidencia. gov.co/normativa/normativa/DECRETO%20457%20 DEL%2022%20DE%20MARZO%20DE%202020.pdfMinistry of Transport, Colombia. Decree 439 [in Spanish]. 2020 Mar 20 [cited 2020 May 10] https://dapre.presidencia. gov.co/normativa/normativa/DECRETO%20439%20 DEL%2020%20DE%20MARZO%20DE%202020.pdfChancellery of Colombia. Communication on humanitarian flights from 2 to 12 July; 2020 June 17 [cited 2020 Jun 19]. https://www.cancilleria.gov.co/newsroom/publiques/ comunicado-vuelos-caracter-humanitario-2-12-julioSun K, Chen J, Viboud C. Early epidemiological analysis of the coronavirus disease 2019 outbreak based on crowdsourced data: a population-level observational study. Lancet Digit Health. 2020;2:e201–8. https://doi.org/10.1016/ s2589-7500(20)30026-1Castillo AE, Parra B, Tapia P, Acevedo A, Lagos J, Andrade W, et al. Phylogenetic analysis of the first four SARS-CoV-2 cases in Chile. J Med Virol. 2020;92:1562–6. https://doi.org/10.1002/jmv.25797Candido DS, Claro IM, de Jesus JG, Souza WM, Moreira FRR, Dellicour S, et al.; Brazil-UK Centre for Arbovirus Discovery, Diagnosis, Genomics and Epidemiology (CADDE) Genomic Network. Evolution and epidemic spread of SARS-CoV-2 in Brazil. Science. 2020;369:1255–60. https://doi.org/10.1126/ science.abd2161Korber B, Fischer WM, Gnanakaran S, Yoon H, Theiler J, Abfalterer W, et al.; Sheffield COVID-19 Genomics Group. Tracking changes in SARS-CoV-2 spike: evidence that D614G increases infectivity of the COVID-19 virus. Cell. 2020;182:812–827.e19. https://doi.org/10.1016/ j.cell.2020.06.043da Silva Candido D, Watts A, Abade L, Kraemer MUG, Pybus OG, Croda J, et al. Routes for COVID-19 importation in Brazil. J Travel Med. 2020;27:taaa042. https://doi.org/ 10.1093/jtm/taaa042CoronavirusSecuenciaciónFilogenómicaCOVID-19ColombiaSARS-CoV-2Genome sequencingPhylogenomicsCOVID-19ColombiaGenomic epidemiology of severe acute Respiratory Syndrome Coronavirus 2, ColombiaArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribucióninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationORIGINAL31. Genomic epidemiology of severe acute respiratory syndrome coronavirus 2 in Colombia. Laiton-Donato et al 2020.pdf31. Genomic epidemiology of severe acute respiratory syndrome coronavirus 2 in Colombia. 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