Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019

Dengue is an endemic disease in Colombia. Norte de Santander is a region on the border of Colombia and Venezuela and has reported the co-circulation and simultaneous co-infection of different serotypes of the dengue virus (DENV). This study aimed to conduct a phylogenetic analysis on the origin and...

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Autores:
Carrillo Hernández, Marlen
Ruiz Sáenz, Julián
Jaimes, Lucy
Robledo, Sara
Martínez Gutiérrez, Marlén
Tipo de recurso:
Article of investigation
Fecha de publicación:
2021
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
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OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/43401
Acceso en línea:
https://hdl.handle.net/20.500.12494/43401
Palabra clave:
Phylogenetic analysis
Venezuela
Phylogeography
Dengue fever
Dengue virus
Phylogenetics
Polymerase chain reaction
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openAccess
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id COOPER2_367c5b6c9b1c0a103a3550c51e292e94
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/43401
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
dc.title.spa.fl_str_mv Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019
title Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019
spellingShingle Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019
Phylogenetic analysis
Venezuela
Phylogeography
Dengue fever
Dengue virus
Phylogenetics
Polymerase chain reaction
title_short Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019
title_full Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019
title_fullStr Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019
title_full_unstemmed Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019
title_sort Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019
dc.creator.fl_str_mv Carrillo Hernández, Marlen
Ruiz Sáenz, Julián
Jaimes, Lucy
Robledo, Sara
Martínez Gutiérrez, Marlén
dc.contributor.author.none.fl_str_mv Carrillo Hernández, Marlen
Ruiz Sáenz, Julián
Jaimes, Lucy
Robledo, Sara
Martínez Gutiérrez, Marlén
dc.subject.spa.fl_str_mv Phylogenetic analysis
Venezuela
Phylogeography
Dengue fever
Dengue virus
Phylogenetics
Polymerase chain reaction
topic Phylogenetic analysis
Venezuela
Phylogeography
Dengue fever
Dengue virus
Phylogenetics
Polymerase chain reaction
description Dengue is an endemic disease in Colombia. Norte de Santander is a region on the border of Colombia and Venezuela and has reported the co-circulation and simultaneous co-infection of different serotypes of the dengue virus (DENV). This study aimed to conduct a phylogenetic analysis on the origin and genetic diversity of DENV strains circulating in this bordering region. Serum samples were collected from patients who were clinically diagnosed with febrile syndrome associated with dengue during two periods. These samples were tested for DENV and serotyping was performed using reverse transcriptase-polymerase chain reaction. Subsequently, positive samples were amplified and the envelope protein gene of DENV was sequenced. Phylogenetic and phylogeographic analyses were performed using the sequences obtained. Basic local alignment search tool analysis confirmed that six and eight sequences belonged to DENV-1 and DENV-2, respectively. The phylogenetic analysis of DENV-1 showed that the sequences belonged to genotype V and clade I; they formed two groups: in the first group, two sequences showed a close phylogenetic relationship with strains from Ecuador and Panama, whereas the other four sequences were grouped with strains from Venezuela and Colombia. In the case of DENV-2, the analysis revealed that the sequences belonged to the Asian–American genotype and clade III. Furthermore, they formed two groups; in the first group, three sequences were grouped with strains from Colombia and Venezuela, whereas the other five were grouped with strains from Venezuela, Colombia and Honduras. This phylogenetic analysis suggests that the geographical proximity between Colombia and Venezuela is favourable for the export and import of different strains among serotypes or clades of the same DENV serotype, which could favour the spread of new outbreaks caused by new strains or genetic variants of this arbovirus. Therefore, this information highlights the importance of monitoring the transmission of DENV at border regions.
publishDate 2021
dc.date.issued.none.fl_str_mv 2021-05
dc.date.accessioned.none.fl_str_mv 2022-01-24T20:54:38Z
dc.date.available.none.fl_str_mv 2022-01-24T20:54:38Z
dc.type.none.fl_str_mv Artículos Científicos
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dc.identifier.issn.spa.fl_str_mv 1932-6203
dc.identifier.uri.spa.fl_str_mv doi.org/10.1371/journal.pone.0252379
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/43401
dc.identifier.bibliographicCitation.spa.fl_str_mv Carrillo-Hernandez MY, Ruiz-Saenz J, Jaimes-Villamizar L, Robledo-Restrepo SM, Martinez-Gutierrez M (2021) Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019. PLOS ONE 16(5): e0252379. https://doi.org/10.1371/journal.pone.0252379
identifier_str_mv 1932-6203
doi.org/10.1371/journal.pone.0252379
Carrillo-Hernandez MY, Ruiz-Saenz J, Jaimes-Villamizar L, Robledo-Restrepo SM, Martinez-Gutierrez M (2021) Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019. PLOS ONE 16(5): e0252379. https://doi.org/10.1371/journal.pone.0252379
url https://hdl.handle.net/20.500.12494/43401
dc.relation.isversionof.spa.fl_str_mv https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252379
dc.relation.ispartofjournal.spa.fl_str_mv PLOS ONE
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Perez-Ramirez G, Diaz-Badillo A, Camacho-Nuez M, Cisneros A, de Lourdes Munoz M. Multiple recombinants in two dengue virus, serotype-2 isolates from patients from Oaxaca, Mexico. BMC microbiology. 2009;9(1):260. pmid:20003526
Costa RL, Voloch CM, Schrago CG. Comparative evolutionary epidemiology of dengue virus serotypes. Infection, Genetics and Evolution. 2012;12(2):309–14. pmid:22226705
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Franco L, Pagan I, Del Cor NS, Schunk M, Neumayr A, Molero F, et al. Molecular epidemiology suggests Venezuela as the origin of the dengue outbreak in Madeira, Portugal in 2012–2013. Clinical microbiology and infection. 2015;21(7):713. e5–. e8. pmid:25843502
Ospina MC, Diaz FJ, Osorio JE. Prolonged co-circulation of two distinct Dengue virus Type 3 lineages in the hyperendemic area of Medellin, Colombia. The American journal of tropical medicine and hygiene. 2010;83(3):672–8. pmid:20810837
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spelling Carrillo Hernández, MarlenRuiz Sáenz, JuliánJaimes, LucyRobledo, SaraMartínez Gutiérrez, Marlén16 p.2022-01-24T20:54:38Z2022-01-24T20:54:38Z2021-051932-6203doi.org/10.1371/journal.pone.0252379https://hdl.handle.net/20.500.12494/43401Carrillo-Hernandez MY, Ruiz-Saenz J, Jaimes-Villamizar L, Robledo-Restrepo SM, Martinez-Gutierrez M (2021) Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019. PLOS ONE 16(5): e0252379. https://doi.org/10.1371/journal.pone.0252379Dengue is an endemic disease in Colombia. Norte de Santander is a region on the border of Colombia and Venezuela and has reported the co-circulation and simultaneous co-infection of different serotypes of the dengue virus (DENV). This study aimed to conduct a phylogenetic analysis on the origin and genetic diversity of DENV strains circulating in this bordering region. Serum samples were collected from patients who were clinically diagnosed with febrile syndrome associated with dengue during two periods. These samples were tested for DENV and serotyping was performed using reverse transcriptase-polymerase chain reaction. Subsequently, positive samples were amplified and the envelope protein gene of DENV was sequenced. Phylogenetic and phylogeographic analyses were performed using the sequences obtained. Basic local alignment search tool analysis confirmed that six and eight sequences belonged to DENV-1 and DENV-2, respectively. The phylogenetic analysis of DENV-1 showed that the sequences belonged to genotype V and clade I; they formed two groups: in the first group, two sequences showed a close phylogenetic relationship with strains from Ecuador and Panama, whereas the other four sequences were grouped with strains from Venezuela and Colombia. In the case of DENV-2, the analysis revealed that the sequences belonged to the Asian–American genotype and clade III. Furthermore, they formed two groups; in the first group, three sequences were grouped with strains from Colombia and Venezuela, whereas the other five were grouped with strains from Venezuela, Colombia and Honduras. This phylogenetic analysis suggests that the geographical proximity between Colombia and Venezuela is favourable for the export and import of different strains among serotypes or clades of the same DENV serotype, which could favour the spread of new outbreaks caused by new strains or genetic variants of this arbovirus. Therefore, this information highlights the importance of monitoring the transmission of DENV at border regions.http://scienti.colciencias.gov.co:8081/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000153095https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000213748https://orcid.org/0000-0002-9429-00580000-0002-1447-1458https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000000695Marlen.martinezg@campucucc.edu.cojulian.ruizs@campusucc.edu.cohttps://scholar.google.es/citations?user=flSrsSIAAAAJ&hl=eshttps://scholar.google.com/citations?hl=es&user=a4L-Fx0AAAAJe0252379Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, BucaramangaPLOSMedicina veterinaria y zootecniaBucaramangahttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252379PLOS ONENoor R. Reemergence of dengue virus in Bangladesh: Current fatality and the required knowledge. Tzu Chi Medical Journal. 2020;32(3):227. pmid:32955510Waman VP, Kolekar P, Ramtirthkar MR, Kale MM, Kulkarni-Kale U. Analysis of genotype diversity and evolution of Dengue virus serotype 2 using complete genomes. PeerJ. 2016;4:e2326. pmid:27635316Perez-Ramirez G, Diaz-Badillo A, Camacho-Nuez M, Cisneros A, de Lourdes Munoz M. Multiple recombinants in two dengue virus, serotype-2 isolates from patients from Oaxaca, Mexico. BMC microbiology. 2009;9(1):260. pmid:20003526Costa RL, Voloch CM, Schrago CG. Comparative evolutionary epidemiology of dengue virus serotypes. Infection, Genetics and Evolution. 2012;12(2):309–14. pmid:22226705Vicente C, Pannuti C, Urbano P, Felix A, JUNIOR CC, Herbinger K-H, et al. First phylogenetic analysis of dengue virus serotype 4 circulating in Espírito Santo state, Brazil, in 2013 and 2014. Epidemiology & Infection. 2018;146(1):100–6.OMS, TDR, OPS. Dengue: guias para el diagnóstico, tratamiento, prevención y control. 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