Proteomic profile of human monocytic cells infected with dengue virus
Objective To identify the changes in the proteome of U937 cells infected with dengue virus (DENV). Methods In this study, differentiated U937 cultures were infected with two DENV-2 strains, one of which was associated with dengue (DENV-2/NG) and the other one with severe dengue (DENV-2/16681), with...
- Autores:
-
Martínez-Betancur, V.
Martínez Gutiérrez, Marlén
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2016
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/41421
- Acceso en línea:
- https://doi.org/10.1016/j.endinu.2016.09.002
https://hdl.handle.net/20.500.12494/41421
- Palabra clave:
- beta tubulin
cell protein
enolase
fatty acid binding protein
heat shock protein
heat shock protein 90
heterogeneous nuclear ribonucleoprotein
lipocortin 4
phospholipase C
protein disulfide isomerase
proteome
pyruvate kinase
transaldolase
viral protein
virus DNA
Article
cell viability
controlled study
Dengue virus 2
drug targeting
human
human cell
mass spectrometry
monocyte
nonhuman
pathogenicity
priority journal
protein analysis
protein expression
proteomics
two dimensional electrophoresis
U937 cell line
virus particle
- Rights
- closedAccess
- License
- http://purl.org/coar/access_right/c_14cb
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Martínez-Betancur, V.Martínez Gutiérrez, Marlén2021-12-16T22:15:30Z2021-12-16T22:15:30Z2016https://doi.org/10.1016/j.endinu.2016.09.00222211691https://hdl.handle.net/20.500.12494/41421Martínez V,Martínez M. Proteomic profile of human monocytic cells infected with dengue virus. Asian Pac J Trop Biomed. 2016. 6. (11):p. 914-923. .Objective To identify the changes in the proteome of U937 cells infected with dengue virus (DENV). Methods In this study, differentiated U937 cultures were infected with two DENV-2 strains, one of which was associated with dengue (DENV-2/NG) and the other one with severe dengue (DENV-2/16681), with the aim of determining the cellular proteomic profiles under different infection conditions. Cellular proteins were extracted and separated by two-dimensional electrophoresis, and those proteins with differential expression profiles were identified by mass spectrometry. The obtained results were correlated with cellular viability, the number of infectious viral particles, and the viral DNA/protein quantity. Results In comparison with non-infected cultures, in the cells infected with the DENV-2/NG strain, nine proteins were expressed differentially (five were upregulated and four were downregulated); in those cultures infected with the DENV-2/16681 strain, six proteins were differentially expressed (two were downregulated and four were upregulated). The downregulated proteins included fatty acid-binding protein, heterogeneous nuclear ribonucleoprotein 1, protein disulfide isomerase, enolase 1, heat shock 70 kDa protein 9, phosphotyrosyl phosphatase, and annexin IV. The upregulated proteins included heat shock 90 kDa protein AA1, tubulin beta, enolase 1, pyruvate kinase, transaldolase and phospholipase C-alpha. Conclusions Because the monocyte/macrophage lineage is critical for disease pathogenicity, additional studies on these proteins could provide a better understanding of the cellular response to DENV infection and could help identify new therapeutic targets against infection. © 2016 Hainan Medical University0000-0002-9429-0058marlen.martinezg@campusucc.edu.co923-914Hainan Medical Universitybeta tubulincell proteinenolasefatty acid binding proteinheat shock proteinheat shock protein 90heterogeneous nuclear ribonucleoproteinlipocortin 4phospholipase Cprotein disulfide isomeraseproteomepyruvate kinasetransaldolaseviral proteinvirus DNAArticlecell viabilitycontrolled studyDengue virus 2drug targetinghumanhuman cellmass spectrometrymonocytenonhumanpathogenicitypriority journalprotein analysisprotein expressionproteomicstwo dimensional electrophoresisU937 cell linevirus particleProteomic profile of human monocytic cells infected with dengue virusArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionAsian Pacific Journal of Tropical Biomedicineinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/41421oai:repository.ucc.edu.co:20.500.12494/414212024-08-20 16:21:46.929metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com |
dc.title.spa.fl_str_mv |
Proteomic profile of human monocytic cells infected with dengue virus |
title |
Proteomic profile of human monocytic cells infected with dengue virus |
spellingShingle |
Proteomic profile of human monocytic cells infected with dengue virus beta tubulin cell protein enolase fatty acid binding protein heat shock protein heat shock protein 90 heterogeneous nuclear ribonucleoprotein lipocortin 4 phospholipase C protein disulfide isomerase proteome pyruvate kinase transaldolase viral protein virus DNA Article cell viability controlled study Dengue virus 2 drug targeting human human cell mass spectrometry monocyte nonhuman pathogenicity priority journal protein analysis protein expression proteomics two dimensional electrophoresis U937 cell line virus particle |
title_short |
Proteomic profile of human monocytic cells infected with dengue virus |
title_full |
Proteomic profile of human monocytic cells infected with dengue virus |
title_fullStr |
Proteomic profile of human monocytic cells infected with dengue virus |
title_full_unstemmed |
Proteomic profile of human monocytic cells infected with dengue virus |
title_sort |
Proteomic profile of human monocytic cells infected with dengue virus |
dc.creator.fl_str_mv |
Martínez-Betancur, V. Martínez Gutiérrez, Marlén |
dc.contributor.author.none.fl_str_mv |
Martínez-Betancur, V. Martínez Gutiérrez, Marlén |
dc.subject.spa.fl_str_mv |
beta tubulin cell protein enolase fatty acid binding protein heat shock protein heat shock protein 90 heterogeneous nuclear ribonucleoprotein lipocortin 4 phospholipase C protein disulfide isomerase proteome pyruvate kinase transaldolase viral protein virus DNA Article cell viability controlled study Dengue virus 2 drug targeting human human cell mass spectrometry monocyte nonhuman pathogenicity priority journal protein analysis protein expression proteomics two dimensional electrophoresis U937 cell line virus particle |
topic |
beta tubulin cell protein enolase fatty acid binding protein heat shock protein heat shock protein 90 heterogeneous nuclear ribonucleoprotein lipocortin 4 phospholipase C protein disulfide isomerase proteome pyruvate kinase transaldolase viral protein virus DNA Article cell viability controlled study Dengue virus 2 drug targeting human human cell mass spectrometry monocyte nonhuman pathogenicity priority journal protein analysis protein expression proteomics two dimensional electrophoresis U937 cell line virus particle |
description |
Objective To identify the changes in the proteome of U937 cells infected with dengue virus (DENV). Methods In this study, differentiated U937 cultures were infected with two DENV-2 strains, one of which was associated with dengue (DENV-2/NG) and the other one with severe dengue (DENV-2/16681), with the aim of determining the cellular proteomic profiles under different infection conditions. Cellular proteins were extracted and separated by two-dimensional electrophoresis, and those proteins with differential expression profiles were identified by mass spectrometry. The obtained results were correlated with cellular viability, the number of infectious viral particles, and the viral DNA/protein quantity. Results In comparison with non-infected cultures, in the cells infected with the DENV-2/NG strain, nine proteins were expressed differentially (five were upregulated and four were downregulated); in those cultures infected with the DENV-2/16681 strain, six proteins were differentially expressed (two were downregulated and four were upregulated). The downregulated proteins included fatty acid-binding protein, heterogeneous nuclear ribonucleoprotein 1, protein disulfide isomerase, enolase 1, heat shock 70 kDa protein 9, phosphotyrosyl phosphatase, and annexin IV. The upregulated proteins included heat shock 90 kDa protein AA1, tubulin beta, enolase 1, pyruvate kinase, transaldolase and phospholipase C-alpha. Conclusions Because the monocyte/macrophage lineage is critical for disease pathogenicity, additional studies on these proteins could provide a better understanding of the cellular response to DENV infection and could help identify new therapeutic targets against infection. © 2016 Hainan Medical University |
publishDate |
2016 |
dc.date.issued.none.fl_str_mv |
2016 |
dc.date.accessioned.none.fl_str_mv |
2021-12-16T22:15:30Z |
dc.date.available.none.fl_str_mv |
2021-12-16T22:15:30Z |
dc.type.none.fl_str_mv |
Artículo |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.redcol.none.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
dc.type.version.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://doi.org/10.1016/j.endinu.2016.09.002 |
dc.identifier.issn.spa.fl_str_mv |
22211691 |
dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12494/41421 |
dc.identifier.bibliographicCitation.spa.fl_str_mv |
Martínez V,Martínez M. Proteomic profile of human monocytic cells infected with dengue virus. Asian Pac J Trop Biomed. 2016. 6. (11):p. 914-923. . |
url |
https://doi.org/10.1016/j.endinu.2016.09.002 https://hdl.handle.net/20.500.12494/41421 |
identifier_str_mv |
22211691 Martínez V,Martínez M. Proteomic profile of human monocytic cells infected with dengue virus. Asian Pac J Trop Biomed. 2016. 6. (11):p. 914-923. . |
dc.relation.ispartofjournal.spa.fl_str_mv |
Asian Pacific Journal of Tropical Biomedicine |
dc.rights.accessrights.none.fl_str_mv |
info:eu-repo/semantics/closedAccess |
dc.rights.coar.none.fl_str_mv |
http://purl.org/coar/access_right/c_14cb |
eu_rights_str_mv |
closedAccess |
rights_invalid_str_mv |
http://purl.org/coar/access_right/c_14cb |
dc.format.extent.spa.fl_str_mv |
923-914 |
dc.publisher.spa.fl_str_mv |
Hainan Medical University |
institution |
Universidad Cooperativa de Colombia |
repository.name.fl_str_mv |
Repositorio Institucional Universidad Cooperativa de Colombia |
repository.mail.fl_str_mv |
bdigital@metabiblioteca.com |
_version_ |
1814247185755144192 |