Proteomic profile of human monocytic cells infected with dengue virus

Objective To identify the changes in the proteome of U937 cells infected with dengue virus (DENV). Methods In this study, differentiated U937 cultures were infected with two DENV-2 strains, one of which was associated with dengue (DENV-2/NG) and the other one with severe dengue (DENV-2/16681), with...

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Autores:
Martínez-Betancur, V.
Martínez Gutiérrez, Marlén
Tipo de recurso:
Article of journal
Fecha de publicación:
2016
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/41421
Acceso en línea:
https://doi.org/10.1016/j.endinu.2016.09.002
https://hdl.handle.net/20.500.12494/41421
Palabra clave:
beta tubulin
cell protein
enolase
fatty acid binding protein
heat shock protein
heat shock protein 90
heterogeneous nuclear ribonucleoprotein
lipocortin 4
phospholipase C
protein disulfide isomerase
proteome
pyruvate kinase
transaldolase
viral protein
virus DNA
Article
cell viability
controlled study
Dengue virus 2
drug targeting
human
human cell
mass spectrometry
monocyte
nonhuman
pathogenicity
priority journal
protein analysis
protein expression
proteomics
two dimensional electrophoresis
U937 cell line
virus particle
Rights
closedAccess
License
http://purl.org/coar/access_right/c_14cb
id COOPER2_36404a9c36eb0917a17c7a309af8b932
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/41421
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
spelling Martínez-Betancur, V.Martínez Gutiérrez, Marlén2021-12-16T22:15:30Z2021-12-16T22:15:30Z2016https://doi.org/10.1016/j.endinu.2016.09.00222211691https://hdl.handle.net/20.500.12494/41421Martínez V,Martínez M. Proteomic profile of human monocytic cells infected with dengue virus. Asian Pac J Trop Biomed. 2016. 6. (11):p. 914-923. .Objective To identify the changes in the proteome of U937 cells infected with dengue virus (DENV). Methods In this study, differentiated U937 cultures were infected with two DENV-2 strains, one of which was associated with dengue (DENV-2/NG) and the other one with severe dengue (DENV-2/16681), with the aim of determining the cellular proteomic profiles under different infection conditions. Cellular proteins were extracted and separated by two-dimensional electrophoresis, and those proteins with differential expression profiles were identified by mass spectrometry. The obtained results were correlated with cellular viability, the number of infectious viral particles, and the viral DNA/protein quantity. Results In comparison with non-infected cultures, in the cells infected with the DENV-2/NG strain, nine proteins were expressed differentially (five were upregulated and four were downregulated); in those cultures infected with the DENV-2/16681 strain, six proteins were differentially expressed (two were downregulated and four were upregulated). The downregulated proteins included fatty acid-binding protein, heterogeneous nuclear ribonucleoprotein 1, protein disulfide isomerase, enolase 1, heat shock 70 kDa protein 9, phosphotyrosyl phosphatase, and annexin IV. The upregulated proteins included heat shock 90 kDa protein AA1, tubulin beta, enolase 1, pyruvate kinase, transaldolase and phospholipase C-alpha. Conclusions Because the monocyte/macrophage lineage is critical for disease pathogenicity, additional studies on these proteins could provide a better understanding of the cellular response to DENV infection and could help identify new therapeutic targets against infection. © 2016 Hainan Medical University0000-0002-9429-0058marlen.martinezg@campusucc.edu.co923-914Hainan Medical Universitybeta tubulincell proteinenolasefatty acid binding proteinheat shock proteinheat shock protein 90heterogeneous nuclear ribonucleoproteinlipocortin 4phospholipase Cprotein disulfide isomeraseproteomepyruvate kinasetransaldolaseviral proteinvirus DNAArticlecell viabilitycontrolled studyDengue virus 2drug targetinghumanhuman cellmass spectrometrymonocytenonhumanpathogenicitypriority journalprotein analysisprotein expressionproteomicstwo dimensional electrophoresisU937 cell linevirus particleProteomic profile of human monocytic cells infected with dengue virusArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionAsian Pacific Journal of Tropical Biomedicineinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/41421oai:repository.ucc.edu.co:20.500.12494/414212024-08-20 16:21:46.929metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv Proteomic profile of human monocytic cells infected with dengue virus
title Proteomic profile of human monocytic cells infected with dengue virus
spellingShingle Proteomic profile of human monocytic cells infected with dengue virus
beta tubulin
cell protein
enolase
fatty acid binding protein
heat shock protein
heat shock protein 90
heterogeneous nuclear ribonucleoprotein
lipocortin 4
phospholipase C
protein disulfide isomerase
proteome
pyruvate kinase
transaldolase
viral protein
virus DNA
Article
cell viability
controlled study
Dengue virus 2
drug targeting
human
human cell
mass spectrometry
monocyte
nonhuman
pathogenicity
priority journal
protein analysis
protein expression
proteomics
two dimensional electrophoresis
U937 cell line
virus particle
title_short Proteomic profile of human monocytic cells infected with dengue virus
title_full Proteomic profile of human monocytic cells infected with dengue virus
title_fullStr Proteomic profile of human monocytic cells infected with dengue virus
title_full_unstemmed Proteomic profile of human monocytic cells infected with dengue virus
title_sort Proteomic profile of human monocytic cells infected with dengue virus
dc.creator.fl_str_mv Martínez-Betancur, V.
Martínez Gutiérrez, Marlén
dc.contributor.author.none.fl_str_mv Martínez-Betancur, V.
Martínez Gutiérrez, Marlén
dc.subject.spa.fl_str_mv beta tubulin
cell protein
enolase
fatty acid binding protein
heat shock protein
heat shock protein 90
heterogeneous nuclear ribonucleoprotein
lipocortin 4
phospholipase C
protein disulfide isomerase
proteome
pyruvate kinase
transaldolase
viral protein
virus DNA
Article
cell viability
controlled study
Dengue virus 2
drug targeting
human
human cell
mass spectrometry
monocyte
nonhuman
pathogenicity
priority journal
protein analysis
protein expression
proteomics
two dimensional electrophoresis
U937 cell line
virus particle
topic beta tubulin
cell protein
enolase
fatty acid binding protein
heat shock protein
heat shock protein 90
heterogeneous nuclear ribonucleoprotein
lipocortin 4
phospholipase C
protein disulfide isomerase
proteome
pyruvate kinase
transaldolase
viral protein
virus DNA
Article
cell viability
controlled study
Dengue virus 2
drug targeting
human
human cell
mass spectrometry
monocyte
nonhuman
pathogenicity
priority journal
protein analysis
protein expression
proteomics
two dimensional electrophoresis
U937 cell line
virus particle
description Objective To identify the changes in the proteome of U937 cells infected with dengue virus (DENV). Methods In this study, differentiated U937 cultures were infected with two DENV-2 strains, one of which was associated with dengue (DENV-2/NG) and the other one with severe dengue (DENV-2/16681), with the aim of determining the cellular proteomic profiles under different infection conditions. Cellular proteins were extracted and separated by two-dimensional electrophoresis, and those proteins with differential expression profiles were identified by mass spectrometry. The obtained results were correlated with cellular viability, the number of infectious viral particles, and the viral DNA/protein quantity. Results In comparison with non-infected cultures, in the cells infected with the DENV-2/NG strain, nine proteins were expressed differentially (five were upregulated and four were downregulated); in those cultures infected with the DENV-2/16681 strain, six proteins were differentially expressed (two were downregulated and four were upregulated). The downregulated proteins included fatty acid-binding protein, heterogeneous nuclear ribonucleoprotein 1, protein disulfide isomerase, enolase 1, heat shock 70 kDa protein 9, phosphotyrosyl phosphatase, and annexin IV. The upregulated proteins included heat shock 90 kDa protein AA1, tubulin beta, enolase 1, pyruvate kinase, transaldolase and phospholipase C-alpha. Conclusions Because the monocyte/macrophage lineage is critical for disease pathogenicity, additional studies on these proteins could provide a better understanding of the cellular response to DENV infection and could help identify new therapeutic targets against infection. © 2016 Hainan Medical University
publishDate 2016
dc.date.issued.none.fl_str_mv 2016
dc.date.accessioned.none.fl_str_mv 2021-12-16T22:15:30Z
dc.date.available.none.fl_str_mv 2021-12-16T22:15:30Z
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.endinu.2016.09.002
dc.identifier.issn.spa.fl_str_mv 22211691
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/41421
dc.identifier.bibliographicCitation.spa.fl_str_mv Martínez V,Martínez M. Proteomic profile of human monocytic cells infected with dengue virus. Asian Pac J Trop Biomed. 2016. 6. (11):p. 914-923. .
url https://doi.org/10.1016/j.endinu.2016.09.002
https://hdl.handle.net/20.500.12494/41421
identifier_str_mv 22211691
Martínez V,Martínez M. Proteomic profile of human monocytic cells infected with dengue virus. Asian Pac J Trop Biomed. 2016. 6. (11):p. 914-923. .
dc.relation.ispartofjournal.spa.fl_str_mv Asian Pacific Journal of Tropical Biomedicine
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/closedAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_14cb
eu_rights_str_mv closedAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_14cb
dc.format.extent.spa.fl_str_mv 923-914
dc.publisher.spa.fl_str_mv Hainan Medical University
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
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