Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli
Antecedentes: El objetivo de este estudio fue medir la respuesta inflamatoria de la pulpa dental a través de neuropéptidos (SP y CGRP) como respuesta al trauma oclusal, movimientos de ortodoncia y una combinación de ambos, así como el mecanismo de defensa angiogénico a través de la expresión de VEGF...
- Autores:
-
Muñoz Alvear, Hernán Dario
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2021
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/46725
- Acceso en línea:
- https://doi.org/10.1186/s12903-021-01519-x
https://hdl.handle.net/20.500.12494/46725
- Palabra clave:
- SP
CGRP
VEGF
Trauma oclusal
Fuerza de ortodoncia
Inflamación neurogénica
SP
CGRP
VEGF
Occlusal trauma
Orthodontics force
Neurogenic inflammation
- Rights
- openAccess
- License
- Atribución – Compartir igual
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| dc.title.spa.fl_str_mv |
Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli |
| title |
Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli |
| spellingShingle |
Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli SP CGRP VEGF Trauma oclusal Fuerza de ortodoncia Inflamación neurogénica SP CGRP VEGF Occlusal trauma Orthodontics force Neurogenic inflammation |
| title_short |
Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli |
| title_full |
Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli |
| title_fullStr |
Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli |
| title_full_unstemmed |
Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli |
| title_sort |
Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli |
| dc.creator.fl_str_mv |
Muñoz Alvear, Hernán Dario |
| dc.contributor.author.none.fl_str_mv |
Muñoz Alvear, Hernán Dario |
| dc.subject.spa.fl_str_mv |
SP CGRP VEGF Trauma oclusal Fuerza de ortodoncia Inflamación neurogénica |
| topic |
SP CGRP VEGF Trauma oclusal Fuerza de ortodoncia Inflamación neurogénica SP CGRP VEGF Occlusal trauma Orthodontics force Neurogenic inflammation |
| dc.subject.other.spa.fl_str_mv |
SP CGRP VEGF Occlusal trauma Orthodontics force Neurogenic inflammation |
| description |
Antecedentes: El objetivo de este estudio fue medir la respuesta inflamatoria de la pulpa dental a través de neuropéptidos (SP y CGRP) como respuesta al trauma oclusal, movimientos de ortodoncia y una combinación de ambos, así como el mecanismo de defensa angiogénico a través de la expresión de VEGF, que podría ser el paso inicial para la formación de tejido mineralizado. Métodos: Se recolectaron cuarenta muestras de pulpa dental humana de primeros premolares sanos con extracción indicada por motivos de ortodoncia de una muestra de 20 pacientes. Los pacientes se dividieron en cuatro grupos con 10 premolares cada uno (1 premolar mandibular y 1 premolar maxilar de cada paciente): grupo de control de pulpa sana, grupo de trauma oclusal, grupo de fuerzas de ortodoncia moderadas; y grupo de trauma oclusal más fuerzas de ortodoncia moderadas. Se aplicaron estímulos durante 24 h antes de la extracción dental en todos los grupos experimentales. Se procesaron todas las muestras y se midieron SP, CGRP y VEGF mediante radioinmunoensayo. Se realizó la prueba de Kruskal-Wallis para evaluar las diferencias significativas entre los grupos y también se realizaron comparaciones por pares post hoc de la U de Mann-Whitney. Resultados: El mayor aumento en las expresiones de SP, CGRP y VEGF se encontró en el grupo de trauma oclusal más fuerzas de ortodoncia, seguido por las fuerzas de ortodoncia moderadas, el trauma oclusal y los grupos control, con diferencias estadísticamente significativas entre todos los grupos para cada grupo. de los 3 péptidos analizados (Kruskal-Wallis p < 0,001). Todas las comparaciones post-hoc por pares posibles también fueron significativas para cada péptido analizado (Mann-Whitney's Up < 0,001). Conclusión: Las expresiones de SP, CGRP y VEGF aumentan significativamente en las pulpas dentales humanas cuando son estimuladas por un traumatismo oclusal combinado con fuerzas ortodóncicas moderadas, en comparación con estos dos estímulos aplicados de forma independiente. |
| publishDate |
2021 |
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2021-03 |
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2022-10-13T23:28:25Z |
| dc.date.available.none.fl_str_mv |
2022-10-13T23:28:25Z |
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Artículos Científicos |
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1472-6831 |
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https://doi.org/10.1186/s12903-021-01519-x |
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https://hdl.handle.net/20.500.12494/46725 |
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Caviedes-Bucheli, J., Lopez-Moncayo, L. F., Muñoz-Alvear, H. D., Gomez-Sosa, J. F., Diaz-Barrera, L. E., Curtidor, H., & Munoz, H. R. (2021). Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli. BMC oral health, 21(1), 152.https://repository.ucc.edu.co/handle/20.500.12494/46725 |
| identifier_str_mv |
1472-6831 Caviedes-Bucheli, J., Lopez-Moncayo, L. F., Muñoz-Alvear, H. D., Gomez-Sosa, J. F., Diaz-Barrera, L. E., Curtidor, H., & Munoz, H. R. (2021). Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli. BMC oral health, 21(1), 152.https://repository.ucc.edu.co/handle/20.500.12494/46725 |
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https://doi.org/10.1186/s12903-021-01519-x https://hdl.handle.net/20.500.12494/46725 |
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https://bmcoralhealth.biomedcentral.com/articles/10.1186/s12903-021-01519-x |
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BMC Oral Health |
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Caviedes‐Bucheli J, Muñoz HR, Azuero‐Holguín MM, Ulate E. Neuropep‐ tides in dental pulp: the silent protagonists. J Endod. 2013;34:773–88. Aranha AM, Zhang Z, Neiva KG, Costa CA, Hebling J, Nör JE. Hypoxia enhances the angiogenic potential of human dental pulp cells. J Endod. 2010;36:1633–7. Lee YH, Kim GE, Cho HJ, Yu MK, Bhattarai G, Lee NH, Yi HK. Aging of in vitro pulp illustrates change of inflammation and dentinogenesis. J Endod. 2013;39:340–5. Caviedes‐Bucheli J, Gomez‐Sosa JF, Azuero‐Holguin MM, Ormeño‐Gomez M, Pinto‐Pascual V, Munoz HR. Angiogenic mechanisms of human dental pulp and their relationship with substance P expression in response to occlusal trauma. Int Endod J. 2017;50:339–51. Killough SA, Lundy FT, Irwin CR. Substance P expression by human dental pulp fibroblasts: a potential role in neurogenic inflammation. J Endod. 2009;35:73–7. Tran‐Hung L, Mathieu S, About I. Role of human pulp fibroblasts in angio‐ genesis. J Dent Res. 2006;85:819–23. El karim IA, Linden GJ, Irwin CR, Lundy FT. Neuropeptides regulate expres‐ sion of angiogenic growth factors in human dental pulp fibroblasts. J Endod. 2009;35:829–33. Uddman R, Kato J, Lindgren P, Sundler F, Edvinsson L. Expression of calci‐ tonin gene‐related peptide‐1 receptor mRNA in human tooth pulp and trigeminal ganglion. Arch Oral Biol. 1999;44:1–6. Tuo Y, Guo X, Zhang X, Wang Z, Zhou J, Xia L, Zhang Y, Wen J, Jin D. The biological effects and mechanisms of calcitonin gene‐related peptide on human endothelial cell. J Recept Signal Transduct. 2013;33:114–23. Grando Mattuella L, Westphalen Bento L, de Figueiredo JA, Nör JE, de Araujo FB, Fossati AC. Vascular endothelial growth factor and its relation‐ ship with the dental pulp. J Endod. 2007;33:524–30. Saghiri MA, Asatourian A, Sorenson CM, Sheibani N. Role of angiogen‐ esis in endodontics: contributions of stem cells and proangiogenic and antiangiogenic factors to dental pulp regeneration. J Endod. 2015;41:797–803. Potente M, Gerhardt H, Carmeliet P. Basic and therapeutic aspects of angiogenesis. Cell. 2011;146:873–87. Wei F, Yang S, Xu H, Guo Q, Li Q, Hu L, Liu D, Wang C. Expression and function of hypoxia inducible factor‐1 α and vascular endothelial growth factor in pulp tissue of teeth under orthodontic movement. Mediators Inflamm. 2015:1–9. Rombouts C, Giraud T, Jeanneau C, About I. Pulp vascularization during tooth development, regeneration, and therapy. J Dent Res. 2017;96:137–44. Ikeda T, Nakano M, Bando E, Suzuki A. The effect of light premature occlusal contact on tooth pain threshold in humans. J Oral Rehabil. 1998;25:589–95. Roberts‐Clark DJ, Smith AJ. Angiogenic growth factors in human dentine matrix. Arch Oral Biol. 2000;45:1013–6. Derringer KA, Linden RWA. Angiogenic growth factors released in human dental pulp following orthodontic force. Arch Oral Biol. 2003;48:285–91. Caviedes‐Bucheli J, Azuero‐Holguin MM, Correa‐Ortiz JA, Aguilar‐Mora MV, Pedroza‐Flores JD, Ulate E, Lombana N, Munoz HR. Effect of experi‐ mentally induced occlusal trauma on substance P expression in human dental pulp and periodontal ligament. J Endod. 2011;37:627–30. Caviedes‐Bucheli J, Moreno JO, Ardila‐Pinto J, Del Toro‐Carreño HR, Saltarín‐Quintero H, Sierra‐Tapias CL, Macias‐Gomez F, Ulate E, Lombana‐Sanchez N, Munoz HR. The effect of orthodontic forces on cal‐ citonin gene‐related peptide expression in human dental pulp. J Endod. 2011;37:934–7. Caviedes‐Bucheli J, Lombana N, Azuero‐Holguin MM, Munoz HR. Quan‐ tification of neuropeptides (calcitonin gene‐related peptide, substance P, neurokinin A, neuropeptide Y and vasoactive intestinal polypeptide) expressed in healthy and inflamed human dental pulp. Int Endod J. 2006;39:394–400. Lundy FT, Linden GJ. Neuropeptides and neurogenic mechanisms in oral and periodontal inflammation. Crit Rev Oral Biol Med. 2004;15:82–98. Artese L, Rubini C, Ferrero G, Fioroni M, Santinelli A, Piattelli A. Vascular endothelial growth factor (VEGF) expression in healthy and inflamed human dental pulps. J Endod. 2002;28:20–3. Mishima T, Ito Y, Hosono K, Tamura Y, Uchida Y, Hirata M, Suzsuki T, Amano H, Kato S, Kurihara Y, Kurihara H, Hayashi I, Watanabe M, Majima M. Calci‐ tonin gene‐related peptide facilitates revascularization during hindlimb ischemia in mice. Am J Physiol ‐ Hear Circ Physiol. 2011;300:H431–9. Rechenberg DK, Galicia JC, Peters OA. Biological markers for pulpal inflammation: A systematic review. PLoS ONE. 2016;11:e0167289. Zheng S, Li W, Xu M, Bai X, Zhou Z, Han J, Shyy JY, Wang X. Calcitonin gene‐related peptide promotes angiogenesis via AMP‐activated protein kinase. Am J Physiol—Cell Physiol. 2010;299:C1485–92. Arana‐Chavez VE, Massa LF. Odontoblasts: The cells forming and main‐ taining dentine. Int J Biochem Cell Biol. 2004;36:1367–73. Sakdee JB, White RR, Pagonis TC, Hauschka PV. Hypoxia‐amplified Prolif‐ eration of Human Dental Pulp Cells. J Endod. 2009;35:818–23. Römer P, Wolf M, Fanghänel J, Reicheneder C, Proff P. Cellular response to orthodontically‐induced short‐term hypoxia in dental pulp cells. Cell Tissue Res. 2014;355:173–80. Olsson M, Lindqvist B. Occlusal interferences in orthodontic patients before and after treatment, and in subjects with minor orthodontic treat‐ ment need. Eur J Orthod. 2002;24:677–87. Ren Y, Maltha JC, Kuijpers‐Jagtman AM. Optimum force magnitude for orthodontic tooth movement: A systematic literature review. Angle Orthod. 2003;73:86–92. Von Böhl M, Ren Y, Fudalej PS, Kuijpers‐Jagtman AM. Pulpal reactions to orthodontic force application in humans: A systematic review. J Endod. 2012;38:1463–9. |
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Departamento de Endodoncia Pasto Jennifer Harman |
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Muñoz Alvear, Hernán Dario212022-10-13T23:28:25Z2022-10-13T23:28:25Z2021-031472-6831https://doi.org/10.1186/s12903-021-01519-xhttps://hdl.handle.net/20.500.12494/46725Caviedes-Bucheli, J., Lopez-Moncayo, L. F., Muñoz-Alvear, H. D., Gomez-Sosa, J. F., Diaz-Barrera, L. E., Curtidor, H., & Munoz, H. R. (2021). Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli. BMC oral health, 21(1), 152.https://repository.ucc.edu.co/handle/20.500.12494/46725Antecedentes: El objetivo de este estudio fue medir la respuesta inflamatoria de la pulpa dental a través de neuropéptidos (SP y CGRP) como respuesta al trauma oclusal, movimientos de ortodoncia y una combinación de ambos, así como el mecanismo de defensa angiogénico a través de la expresión de VEGF, que podría ser el paso inicial para la formación de tejido mineralizado. Métodos: Se recolectaron cuarenta muestras de pulpa dental humana de primeros premolares sanos con extracción indicada por motivos de ortodoncia de una muestra de 20 pacientes. Los pacientes se dividieron en cuatro grupos con 10 premolares cada uno (1 premolar mandibular y 1 premolar maxilar de cada paciente): grupo de control de pulpa sana, grupo de trauma oclusal, grupo de fuerzas de ortodoncia moderadas; y grupo de trauma oclusal más fuerzas de ortodoncia moderadas. Se aplicaron estímulos durante 24 h antes de la extracción dental en todos los grupos experimentales. Se procesaron todas las muestras y se midieron SP, CGRP y VEGF mediante radioinmunoensayo. Se realizó la prueba de Kruskal-Wallis para evaluar las diferencias significativas entre los grupos y también se realizaron comparaciones por pares post hoc de la U de Mann-Whitney. Resultados: El mayor aumento en las expresiones de SP, CGRP y VEGF se encontró en el grupo de trauma oclusal más fuerzas de ortodoncia, seguido por las fuerzas de ortodoncia moderadas, el trauma oclusal y los grupos control, con diferencias estadísticamente significativas entre todos los grupos para cada grupo. de los 3 péptidos analizados (Kruskal-Wallis p < 0,001). Todas las comparaciones post-hoc por pares posibles también fueron significativas para cada péptido analizado (Mann-Whitney's Up < 0,001). Conclusión: Las expresiones de SP, CGRP y VEGF aumentan significativamente en las pulpas dentales humanas cuando son estimuladas por un traumatismo oclusal combinado con fuerzas ortodóncicas moderadas, en comparación con estos dos estímulos aplicados de forma independiente.Background: The aim of this study was to measure the dental pulp inflammatory response through neuropeptides (SP and CGRP) as a response to occlusal trauma, orthodontic movements and a combination of both, as well as the angiogenic defense mechanism through VEGF expression, which could be the initial step to mineralized tissue formation. Methods: Forty human dental pulp samples were collected from healthy first premolars with extraction indicated due to orthodontic reasons from a sample of 20 patients. Patients were divided into four groups with 10 premolars each (1 mandibular and 1 maxillary premolar from each patient): healthy pulp control group, occlusal trauma group, moderate orthodontic forces group; and occlusal trauma plus moderate orthodontic forces group. Stimuli were applied for 24 h before tooth extraction in all experimental groups. All samples were processed, and SP, CGRP, and VEGF were measured by radioimmunoassay. The Kruskal–Wallis test was performed to assess significant differences among groups and Mann–Whitney’s U post hoc pairwise comparisons were also performed. Results: The highest increase in SP, CGRP, and VEGF expressions was found in the occlusal trauma plus orthodontic forces group, followed by the moderate orthodontic forces, the occlusal trauma and the control groups, with statisti‐ cally significant differences between all groups for each of the 3 peptides analyzed (Kruskal–Wallis p < 0.001). All pos‐ sible pairwise post‐hoc comparisons were also significant for each peptide analyzed (Mann–Whitney’s U p < 0.001). Conclusion: SP, CGRP, and VEGF expressions significantly increase in human dental pulps when stimulated by occlusal trauma combined with moderate orthodontic forces, as compared with these two stimuli applied independently.Introducción. -- Métodos. -- Recolección de datos. -- Radioinmunoanalisis RIA. -- Análisis estadístico . -- Resultados. -- Discusión. -- Conclusión.https://scienti.minciencias.gov.co/cvlac/EnRecursoHumano/inicio.dohttps://orcid.org/0000-0002-4327-4242https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000002911hernan.munoz@campusucc.edu.co8Departamento de Endodoncia PastoJennifer HarmanEspecialización en endodonciaPastohttps://bmcoralhealth.biomedcentral.com/articles/10.1186/s12903-021-01519-xBMC Oral HealthCaviedes‐Bucheli J, Muñoz HR, Azuero‐Holguín MM, Ulate E. Neuropep‐ tides in dental pulp: the silent protagonists. J Endod. 2013;34:773–88.Aranha AM, Zhang Z, Neiva KG, Costa CA, Hebling J, Nör JE. Hypoxia enhances the angiogenic potential of human dental pulp cells. J Endod. 2010;36:1633–7.Lee YH, Kim GE, Cho HJ, Yu MK, Bhattarai G, Lee NH, Yi HK. Aging of in vitro pulp illustrates change of inflammation and dentinogenesis. J Endod. 2013;39:340–5.Caviedes‐Bucheli J, Gomez‐Sosa JF, Azuero‐Holguin MM, Ormeño‐Gomez M, Pinto‐Pascual V, Munoz HR. Angiogenic mechanisms of human dental pulp and their relationship with substance P expression in response to occlusal trauma. Int Endod J. 2017;50:339–51.Killough SA, Lundy FT, Irwin CR. Substance P expression by human dental pulp fibroblasts: a potential role in neurogenic inflammation. J Endod. 2009;35:73–7.Tran‐Hung L, Mathieu S, About I. Role of human pulp fibroblasts in angio‐ genesis. J Dent Res. 2006;85:819–23.El karim IA, Linden GJ, Irwin CR, Lundy FT. Neuropeptides regulate expres‐ sion of angiogenic growth factors in human dental pulp fibroblasts. J Endod. 2009;35:829–33.Uddman R, Kato J, Lindgren P, Sundler F, Edvinsson L. Expression of calci‐ tonin gene‐related peptide‐1 receptor mRNA in human tooth pulp and trigeminal ganglion. Arch Oral Biol. 1999;44:1–6.Tuo Y, Guo X, Zhang X, Wang Z, Zhou J, Xia L, Zhang Y, Wen J, Jin D. The biological effects and mechanisms of calcitonin gene‐related peptide on human endothelial cell. J Recept Signal Transduct. 2013;33:114–23.Grando Mattuella L, Westphalen Bento L, de Figueiredo JA, Nör JE, de Araujo FB, Fossati AC. Vascular endothelial growth factor and its relation‐ ship with the dental pulp. J Endod. 2007;33:524–30.Saghiri MA, Asatourian A, Sorenson CM, Sheibani N. Role of angiogen‐ esis in endodontics: contributions of stem cells and proangiogenic and antiangiogenic factors to dental pulp regeneration. J Endod. 2015;41:797–803.Potente M, Gerhardt H, Carmeliet P. Basic and therapeutic aspects of angiogenesis. Cell. 2011;146:873–87.Wei F, Yang S, Xu H, Guo Q, Li Q, Hu L, Liu D, Wang C. Expression and function of hypoxia inducible factor‐1 α and vascular endothelial growth factor in pulp tissue of teeth under orthodontic movement. Mediators Inflamm. 2015:1–9.Rombouts C, Giraud T, Jeanneau C, About I. Pulp vascularization during tooth development, regeneration, and therapy. J Dent Res. 2017;96:137–44.Ikeda T, Nakano M, Bando E, Suzuki A. The effect of light premature occlusal contact on tooth pain threshold in humans. J Oral Rehabil. 1998;25:589–95.Roberts‐Clark DJ, Smith AJ. Angiogenic growth factors in human dentine matrix. Arch Oral Biol. 2000;45:1013–6.Derringer KA, Linden RWA. Angiogenic growth factors released in human dental pulp following orthodontic force. Arch Oral Biol. 2003;48:285–91.Caviedes‐Bucheli J, Azuero‐Holguin MM, Correa‐Ortiz JA, Aguilar‐Mora MV, Pedroza‐Flores JD, Ulate E, Lombana N, Munoz HR. Effect of experi‐ mentally induced occlusal trauma on substance P expression in human dental pulp and periodontal ligament. J Endod. 2011;37:627–30.Caviedes‐Bucheli J, Moreno JO, Ardila‐Pinto J, Del Toro‐Carreño HR, Saltarín‐Quintero H, Sierra‐Tapias CL, Macias‐Gomez F, Ulate E, Lombana‐Sanchez N, Munoz HR. The effect of orthodontic forces on cal‐ citonin gene‐related peptide expression in human dental pulp. J Endod. 2011;37:934–7.Caviedes‐Bucheli J, Lombana N, Azuero‐Holguin MM, Munoz HR. Quan‐ tification of neuropeptides (calcitonin gene‐related peptide, substance P, neurokinin A, neuropeptide Y and vasoactive intestinal polypeptide) expressed in healthy and inflamed human dental pulp. Int Endod J. 2006;39:394–400.Lundy FT, Linden GJ. Neuropeptides and neurogenic mechanisms in oral and periodontal inflammation. Crit Rev Oral Biol Med. 2004;15:82–98.Artese L, Rubini C, Ferrero G, Fioroni M, Santinelli A, Piattelli A. Vascular endothelial growth factor (VEGF) expression in healthy and inflamed human dental pulps. J Endod. 2002;28:20–3.Mishima T, Ito Y, Hosono K, Tamura Y, Uchida Y, Hirata M, Suzsuki T, Amano H, Kato S, Kurihara Y, Kurihara H, Hayashi I, Watanabe M, Majima M. Calci‐ tonin gene‐related peptide facilitates revascularization during hindlimb ischemia in mice. Am J Physiol ‐ Hear Circ Physiol. 2011;300:H431–9.Rechenberg DK, Galicia JC, Peters OA. Biological markers for pulpal inflammation: A systematic review. PLoS ONE. 2016;11:e0167289.Zheng S, Li W, Xu M, Bai X, Zhou Z, Han J, Shyy JY, Wang X. Calcitonin gene‐related peptide promotes angiogenesis via AMP‐activated protein kinase. Am J Physiol—Cell Physiol. 2010;299:C1485–92.Arana‐Chavez VE, Massa LF. Odontoblasts: The cells forming and main‐ taining dentine. Int J Biochem Cell Biol. 2004;36:1367–73.Sakdee JB, White RR, Pagonis TC, Hauschka PV. Hypoxia‐amplified Prolif‐ eration of Human Dental Pulp Cells. J Endod. 2009;35:818–23.Römer P, Wolf M, Fanghänel J, Reicheneder C, Proff P. Cellular response to orthodontically‐induced short‐term hypoxia in dental pulp cells. Cell Tissue Res. 2014;355:173–80.Olsson M, Lindqvist B. Occlusal interferences in orthodontic patients before and after treatment, and in subjects with minor orthodontic treat‐ ment need. Eur J Orthod. 2002;24:677–87.Ren Y, Maltha JC, Kuijpers‐Jagtman AM. Optimum force magnitude for orthodontic tooth movement: A systematic literature review. Angle Orthod. 2003;73:86–92.Von Böhl M, Ren Y, Fudalej PS, Kuijpers‐Jagtman AM. Pulpal reactions to orthodontic force application in humans: A systematic review. J Endod. 2012;38:1463–9.SPCGRPVEGFTrauma oclusalFuerza de ortodonciaInflamación neurogénicaSPCGRPVEGFOcclusal traumaOrthodontics forceNeurogenic inflammationExpression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuliArtículos Científicoshttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribución – Compartir igualinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationORIGINAL2022-caviedesbuchelijavermunozalvearhernandariolopezmoncayoluis fernando-Expression_of_substance_P_calcitonin_gene_related_peptide_and_vascular_endothelial_growth_factor_in_human_dental_pulp_under_different_clinical_stimuli-FormatoPublicacionWeb .pdf2022-caviedesbuchelijavermunozalvearhernandariolopezmoncayoluis 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