Prevalence and Genomic Diversity of Feline Leukemia Virus in Privately Owned and Shelter Cats in Aburrá Valley, Colombia
The feline leukemia virus (FeLV) belongs to the family Retroviridae; it is the first feline retrovirus discovered and one of the agents that has a great impact on cats’ health and the ecology of the feline population worldwide. It is associated with the occurrence of several syndromes of fatal disea...
- Autores:
-
Ortega, Carolina
Valencia, Alida Carolina
Valencia, July
Ruiz Sáenz, Julián
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2020
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/32939
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/32939
- Palabra clave:
- Subgroup classification
Feline viral leukemia
Retrovirus
- Rights
- openAccess
- License
- Atribución – No comercial – Compartir igual
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Prevalence and Genomic Diversity of Feline Leukemia Virus in Privately Owned and Shelter Cats in Aburrá Valley, Colombia |
| title |
Prevalence and Genomic Diversity of Feline Leukemia Virus in Privately Owned and Shelter Cats in Aburrá Valley, Colombia |
| spellingShingle |
Prevalence and Genomic Diversity of Feline Leukemia Virus in Privately Owned and Shelter Cats in Aburrá Valley, Colombia Subgroup classification Feline viral leukemia Retrovirus |
| title_short |
Prevalence and Genomic Diversity of Feline Leukemia Virus in Privately Owned and Shelter Cats in Aburrá Valley, Colombia |
| title_full |
Prevalence and Genomic Diversity of Feline Leukemia Virus in Privately Owned and Shelter Cats in Aburrá Valley, Colombia |
| title_fullStr |
Prevalence and Genomic Diversity of Feline Leukemia Virus in Privately Owned and Shelter Cats in Aburrá Valley, Colombia |
| title_full_unstemmed |
Prevalence and Genomic Diversity of Feline Leukemia Virus in Privately Owned and Shelter Cats in Aburrá Valley, Colombia |
| title_sort |
Prevalence and Genomic Diversity of Feline Leukemia Virus in Privately Owned and Shelter Cats in Aburrá Valley, Colombia |
| dc.creator.fl_str_mv |
Ortega, Carolina Valencia, Alida Carolina Valencia, July Ruiz Sáenz, Julián |
| dc.contributor.author.none.fl_str_mv |
Ortega, Carolina Valencia, Alida Carolina Valencia, July Ruiz Sáenz, Julián |
| dc.subject.spa.fl_str_mv |
Subgroup classification Feline viral leukemia Retrovirus |
| topic |
Subgroup classification Feline viral leukemia Retrovirus |
| description |
The feline leukemia virus (FeLV) belongs to the family Retroviridae; it is the first feline retrovirus discovered and one of the agents that has a great impact on cats’ health and the ecology of the feline population worldwide. It is associated with the occurrence of several syndromes of fatal diseases, including the development of lymphomas. Studies on FeLV have been reported in Colombia, and most of them have been approached from a clinical point of view. However, only a few studies have focused on the prevalence of the infection, while none have clarified which variant or FeLV viral subgroup is presently circulating in our country. Therefore, the present study investigated the prevalence of the infection associated with the molecular characterization of FeLV present in cats in Aburrá Valley, Colombia. The sampling of privately owned and shelter cats was performed in female (n = 54) and male (n = 46) felines; most of them were seemingly healthy according to the owner’s report, with nonspecific clinical history. Immunoassay confirmed that 59.44% (95% confidence interval (CI) = 49.81–69.06%) of felines were FeLV seropositive. The molecular testing of felines using reverse transcription–polymerase chain reaction and sequencing showed that 30% (30/100) of felines were positive, and the most prevalent subgroup in the Aburrá Valley was FeLV-A. In conclusion, the frequency of leukemia virus, as revealed by molecular and serological tests, is one of the highest reported frequencies to date, and a high molecular variation is shown in the Colombian population. More studies on the behaviour of the virus in feline populations in Columbia are warranted to determine its prevalence throughout the country. |
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2020-04 |
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2021-01-28T17:36:52Z |
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2021-01-28T17:36:52Z |
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Ortega, Carolina; Valencia, Alida C.; Duque-Valencia, July; Ruiz-Saenz, Julián. 2020. "Prevalence and Genomic Diversity of Feline Leukemia Virus in Privately Owned and Shelter Cats in Aburrá Valley, Colombia" Viruses 12, no. 4: 464. https://doi.org/10.3390/v12040464 |
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1999-4915 doi.org/10.3390/v12040464 Ortega, Carolina; Valencia, Alida C.; Duque-Valencia, July; Ruiz-Saenz, Julián. 2020. "Prevalence and Genomic Diversity of Feline Leukemia Virus in Privately Owned and Shelter Cats in Aburrá Valley, Colombia" Viruses 12, no. 4: 464. https://doi.org/10.3390/v12040464 |
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Viruses |
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Dunham, S.P.; Graham, E. Retroviral infections of small animals. Vet. Clin. N. Am. Small Anim. Pract. 2008, 38, 879–901 Cattori, V.; Tandon, R.; Riond, B.; Pepin, A.C.; Lutz, H.; Hofmann-Lehmann, R. The kinetics of feline leukaemia virus shedding in experimentally infected cats are associated with infection outcome. Vet. Microbiol. 2009, 133, 292–296. Benveniste, R.E.; Sherr, C.J.; Todaro, G.J. Evolution of type C viral genes: Origin of feline leukemia virus. Science 1975, 190, 886–888 Chiu, E.S.; Hoover, E.A.; VandeWoude, S. A Retrospective Examination of Feline Leukemia Subgroup Characterization: Viral Interference Assays to Deep Sequencing. Viruses 2018, 10, 29. Willett, B.J.; Hosie, M.J. Feline leukaemia virus: Half a century since its discovery. Vet. J. 2013, 195, 16–23 Sheets, R.L.; Pandey, R.; Klement, V.; Grant, C.K.; Roy-burman, P. Biologically selected recombinants between feline leukemia virus (FeLV) subgroup A and an endogenous FeLV element. Virology 1992, 190, 849–855 Stewart, H.; Adema, K.W.; McMonagle, E.L.; Hosie, M.J.; Willett, B.J. Identification of novel subgroup A variants with enhanced receptor binding and replicative capacity in primary isolates of anaemogenic strains of feline leukaemia virus. Retrovirology 2012, 9, 48 Anai, Y.; Ochi, H.; Watanabe, S.; Nakagawa, S.; Kawamura, M.; Gojobori, T.; Nishigaki, K. Infectious endogenous retroviruses in cats and emergence of recombinant viruses. J. Virol. 2012, 86, 8634–8644 Anderson, M.M.; Lauring, A.S.; Burns, C.C.; Overbaugh, J. Identification of a cellular cofactor required for infection by feline leukemia virus. Science 2000, 287, 1828–1830 Miyake, A.; Watanabe, S.; Hiratsuka, T.; Ito, J.; Ngo, M.H.; Makundi, I.; Kawasaki, J.; Endo, Y.; Tsujimoto, H.; Nishigaki, K. Novel Feline Leukemia Virus Interference Group Based on the env Gene. J. Virol. 2016, 90, 4832–4837 Hartmann, K. Clinical aspects of feline retroviruses: A review. Viruses 2012, 4, 2684–2710. Munoz, L. Haemopoyetic neoplasm in 10 cats positive to feline leukaemia virus. Arch. Med. Vet. 2005, 37, 71–76 Belgard, S.; Truyen, U.; Thibault, J.C.; Sauter-Louis, C.; Hartmann, K. Relevance of feline calicivirus, feline immunodeficiency virus, feline leukemia virus, feline herpesvirus and Bartonella henselae in cats with chronic gingivostomatitis. Berl. Munch Tierarztl. Wochenschr. 2010, 123, 369–376 Quimby, J.M.; Elston, T.; Hawley, J.; Brewer, M.; Miller, A.; Lappin, M.R. Evaluation of the association of Bartonella species, feline herpesvirus 1, feline calicivirus, feline leukemia virus and feline immunodeficiency virus with chronic feline gingivostomatitis. J. Feline Med. Surg. 2008, 10, 66–72. Powers, J.A.; Chiu, E.S.; Kraberger, S.J.; Roelke-Parker, M.; Lowery, I.; Erbeck, K.; Troyer, R.; Carver, S.; VandeWoude, S. Feline Leukemia Virus (FeLV) Disease Outcomes in a Domestic Cat Breeding Colony: Relationship to Endogenous FeLV and Other Chronic Viral Infections. J. Virol. 2018, 92. |
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Ortega, CarolinaValencia, Alida Carolina Valencia, JulyRuiz Sáenz, Julián12(4)2021-01-28T17:36:52Z2021-01-28T17:36:52Z2020-041999-4915doi.org/10.3390/v12040464https://hdl.handle.net/20.500.12494/32939Ortega, Carolina; Valencia, Alida C.; Duque-Valencia, July; Ruiz-Saenz, Julián. 2020. "Prevalence and Genomic Diversity of Feline Leukemia Virus in Privately Owned and Shelter Cats in Aburrá Valley, Colombia" Viruses 12, no. 4: 464. https://doi.org/10.3390/v12040464The feline leukemia virus (FeLV) belongs to the family Retroviridae; it is the first feline retrovirus discovered and one of the agents that has a great impact on cats’ health and the ecology of the feline population worldwide. It is associated with the occurrence of several syndromes of fatal diseases, including the development of lymphomas. Studies on FeLV have been reported in Colombia, and most of them have been approached from a clinical point of view. However, only a few studies have focused on the prevalence of the infection, while none have clarified which variant or FeLV viral subgroup is presently circulating in our country. Therefore, the present study investigated the prevalence of the infection associated with the molecular characterization of FeLV present in cats in Aburrá Valley, Colombia. The sampling of privately owned and shelter cats was performed in female (n = 54) and male (n = 46) felines; most of them were seemingly healthy according to the owner’s report, with nonspecific clinical history. Immunoassay confirmed that 59.44% (95% confidence interval (CI) = 49.81–69.06%) of felines were FeLV seropositive. The molecular testing of felines using reverse transcription–polymerase chain reaction and sequencing showed that 30% (30/100) of felines were positive, and the most prevalent subgroup in the Aburrá Valley was FeLV-A. In conclusion, the frequency of leukemia virus, as revealed by molecular and serological tests, is one of the highest reported frequencies to date, and a high molecular variation is shown in the Colombian population. More studies on the behaviour of the virus in feline populations in Columbia are warranted to determine its prevalence throughout the country.http://scienti.colciencias.gov.co:8081/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000153095https://orcid.org/0000-0002-1447-1458https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000000695julian.ruizs@campusucc.edu.cohttps://scholar.google.com/citations?user=o3Y7mZwAAAAJ&hl=es464MDPIUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, BucaramangaMedicina veterinaria y zootecniaBucaramangahttps://www.mdpi.com/1999-4915/12/4/464VirusesDunham, S.P.; Graham, E. Retroviral infections of small animals. Vet. Clin. N. Am. Small Anim. Pract. 2008, 38, 879–901Cattori, V.; Tandon, R.; Riond, B.; Pepin, A.C.; Lutz, H.; Hofmann-Lehmann, R. The kinetics of feline leukaemia virus shedding in experimentally infected cats are associated with infection outcome. Vet. Microbiol. 2009, 133, 292–296.Benveniste, R.E.; Sherr, C.J.; Todaro, G.J. Evolution of type C viral genes: Origin of feline leukemia virus. Science 1975, 190, 886–888Chiu, E.S.; Hoover, E.A.; VandeWoude, S. A Retrospective Examination of Feline Leukemia Subgroup Characterization: Viral Interference Assays to Deep Sequencing. Viruses 2018, 10, 29.Willett, B.J.; Hosie, M.J. Feline leukaemia virus: Half a century since its discovery. Vet. J. 2013, 195, 16–23Sheets, R.L.; Pandey, R.; Klement, V.; Grant, C.K.; Roy-burman, P. Biologically selected recombinants between feline leukemia virus (FeLV) subgroup A and an endogenous FeLV element. Virology 1992, 190, 849–855Stewart, H.; Adema, K.W.; McMonagle, E.L.; Hosie, M.J.; Willett, B.J. Identification of novel subgroup A variants with enhanced receptor binding and replicative capacity in primary isolates of anaemogenic strains of feline leukaemia virus. Retrovirology 2012, 9, 48Anai, Y.; Ochi, H.; Watanabe, S.; Nakagawa, S.; Kawamura, M.; Gojobori, T.; Nishigaki, K. Infectious endogenous retroviruses in cats and emergence of recombinant viruses. J. Virol. 2012, 86, 8634–8644Anderson, M.M.; Lauring, A.S.; Burns, C.C.; Overbaugh, J. Identification of a cellular cofactor required for infection by feline leukemia virus. Science 2000, 287, 1828–1830Miyake, A.; Watanabe, S.; Hiratsuka, T.; Ito, J.; Ngo, M.H.; Makundi, I.; Kawasaki, J.; Endo, Y.; Tsujimoto, H.; Nishigaki, K. Novel Feline Leukemia Virus Interference Group Based on the env Gene. J. Virol. 2016, 90, 4832–4837Hartmann, K. Clinical aspects of feline retroviruses: A review. Viruses 2012, 4, 2684–2710.Munoz, L. Haemopoyetic neoplasm in 10 cats positive to feline leukaemia virus. Arch. Med. Vet. 2005, 37, 71–76Belgard, S.; Truyen, U.; Thibault, J.C.; Sauter-Louis, C.; Hartmann, K. Relevance of feline calicivirus, feline immunodeficiency virus, feline leukemia virus, feline herpesvirus and Bartonella henselae in cats with chronic gingivostomatitis. Berl. Munch Tierarztl. Wochenschr. 2010, 123, 369–376Quimby, J.M.; Elston, T.; Hawley, J.; Brewer, M.; Miller, A.; Lappin, M.R. Evaluation of the association of Bartonella species, feline herpesvirus 1, feline calicivirus, feline leukemia virus and feline immunodeficiency virus with chronic feline gingivostomatitis. J. Feline Med. Surg. 2008, 10, 66–72.Powers, J.A.; Chiu, E.S.; Kraberger, S.J.; Roelke-Parker, M.; Lowery, I.; Erbeck, K.; Troyer, R.; Carver, S.; VandeWoude, S. Feline Leukemia Virus (FeLV) Disease Outcomes in a Domestic Cat Breeding Colony: Relationship to Endogenous FeLV and Other Chronic Viral Infections. J. 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