Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga

Theraphosidae spider venoms are well known for possess a complex mixture of protein and non-protein compounds in their venom. The objective of this study was to report and identify different proteins translated from the venom gland DNA information of the recently described Theraphosidae spider Pamph...

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Autores:
Estrada-Gómez S.
Vargas Muñoz, Leidy Johana
Saldarriaga-Córdoba M.
Cifuentes Y.
Perafan C.
Tipo de recurso:
Article of journal
Fecha de publicación:
2017
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/41610
Acceso en línea:
https://doi.org/10.1155/2018/4219625
https://www.sciencedirect.com/science/article/abs/pii/S0122726218300417?via%3Dihub
https://hdl.handle.net/20.500.12494/41610
Palabra clave:
Pamphobeteus verdolaga
Proteomic
Theraphosidae
Transcriptomic
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closedAccess
License
http://purl.org/coar/access_right/c_14cb
id COOPER2_2e37043aa704bc8760d10673c3542165
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/41610
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
spelling Estrada-Gómez S.Vargas Muñoz, Leidy JohanaSaldarriaga-Córdoba M.Cifuentes Y.Perafan C.2021-12-16T22:15:39Z2021-12-16T22:15:39Z2017https://doi.org/10.1155/2018/4219625https://www.sciencedirect.com/science/article/abs/pii/S0122726218300417?via%3Dihub00410101https://hdl.handle.net/20.500.12494/41610Estrada S,Vargas LJ,Saldarriaga M,Cifuentes Y,Perafan C. Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga. Toxicon. 2017. 129. p. 81-88. .Theraphosidae spider venoms are well known for possess a complex mixture of protein and non-protein compounds in their venom. The objective of this study was to report and identify different proteins translated from the venom gland DNA information of the recently described Theraphosidae spider Pamphobeteus verdolaga. Using a venom gland transcriptomic analysis, we reported a set of the first complete sequences of seven different proteins of the recenlty described Theraphosidae spider P. verdolaga. Protein analysis indicates the presence of different proteins on the venom composition of this new spider, some of them uncommon in the Theraphosidae family. MS/MS analysis of P. verdolaga showed different fragments matching sphingomyelinases (sicaritoxin), barytoxins, hexatoxins, latroinsectotoxins, and linear (zadotoxins) peptides. Only four of the MS/MS fragments showed 100% sequence similarity with one of the transcribed proteins. Transcriptomic analysis showed the presence of different groups of proteins like phospholipases, hyaluronidases, inhibitory cysteine knots (ICK) peptides among others. The three database of protein domains used in this study (Pfam, SMART and CDD) showed congruency in the search of unique conserved protein domain for only four of the translated proteins. Those proteins matched with EF-hand proteins, cysteine rich secretory proteins, jingzhaotoxins, theraphotoxins and hexatoxins, from different Mygalomorphae spiders belonging to the families Theraphosidae, Barychelidae and Hexathelidae. None of the analyzed sequences showed a complete 100% similarity. © 2017 Elsevier Ltdleidy.vargasmu@campusucc.edu.co88-81Elsevier LtdPamphobeteus verdolagaProteomicTheraphosidaeTranscriptomicIdentifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolagaArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionToxiconinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/41610oai:repository.ucc.edu.co:20.500.12494/416102024-08-20 16:18:48.091metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
title Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
spellingShingle Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
Pamphobeteus verdolaga
Proteomic
Theraphosidae
Transcriptomic
title_short Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
title_full Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
title_fullStr Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
title_full_unstemmed Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
title_sort Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
dc.creator.fl_str_mv Estrada-Gómez S.
Vargas Muñoz, Leidy Johana
Saldarriaga-Córdoba M.
Cifuentes Y.
Perafan C.
dc.contributor.author.none.fl_str_mv Estrada-Gómez S.
Vargas Muñoz, Leidy Johana
Saldarriaga-Córdoba M.
Cifuentes Y.
Perafan C.
dc.subject.spa.fl_str_mv Pamphobeteus verdolaga
Proteomic
Theraphosidae
Transcriptomic
topic Pamphobeteus verdolaga
Proteomic
Theraphosidae
Transcriptomic
description Theraphosidae spider venoms are well known for possess a complex mixture of protein and non-protein compounds in their venom. The objective of this study was to report and identify different proteins translated from the venom gland DNA information of the recently described Theraphosidae spider Pamphobeteus verdolaga. Using a venom gland transcriptomic analysis, we reported a set of the first complete sequences of seven different proteins of the recenlty described Theraphosidae spider P. verdolaga. Protein analysis indicates the presence of different proteins on the venom composition of this new spider, some of them uncommon in the Theraphosidae family. MS/MS analysis of P. verdolaga showed different fragments matching sphingomyelinases (sicaritoxin), barytoxins, hexatoxins, latroinsectotoxins, and linear (zadotoxins) peptides. Only four of the MS/MS fragments showed 100% sequence similarity with one of the transcribed proteins. Transcriptomic analysis showed the presence of different groups of proteins like phospholipases, hyaluronidases, inhibitory cysteine knots (ICK) peptides among others. The three database of protein domains used in this study (Pfam, SMART and CDD) showed congruency in the search of unique conserved protein domain for only four of the translated proteins. Those proteins matched with EF-hand proteins, cysteine rich secretory proteins, jingzhaotoxins, theraphotoxins and hexatoxins, from different Mygalomorphae spiders belonging to the families Theraphosidae, Barychelidae and Hexathelidae. None of the analyzed sequences showed a complete 100% similarity. © 2017 Elsevier Ltd
publishDate 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2021-12-16T22:15:39Z
dc.date.available.none.fl_str_mv 2021-12-16T22:15:39Z
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1155/2018/4219625
https://www.sciencedirect.com/science/article/abs/pii/S0122726218300417?via%3Dihub
dc.identifier.issn.spa.fl_str_mv 00410101
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/41610
dc.identifier.bibliographicCitation.spa.fl_str_mv Estrada S,Vargas LJ,Saldarriaga M,Cifuentes Y,Perafan C. Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga. Toxicon. 2017. 129. p. 81-88. .
url https://doi.org/10.1155/2018/4219625
https://www.sciencedirect.com/science/article/abs/pii/S0122726218300417?via%3Dihub
https://hdl.handle.net/20.500.12494/41610
identifier_str_mv 00410101
Estrada S,Vargas LJ,Saldarriaga M,Cifuentes Y,Perafan C. Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga. Toxicon. 2017. 129. p. 81-88. .
dc.relation.ispartofjournal.spa.fl_str_mv Toxicon
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/closedAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_14cb
eu_rights_str_mv closedAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_14cb
dc.format.extent.spa.fl_str_mv 88-81
dc.publisher.spa.fl_str_mv Elsevier Ltd
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
_version_ 1814246890977361920

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