Expression of early angiogenesis indicators in mature versus immature teeth

Antecedentes: El balance adecuado de oxígeno en la pulpa dental es esencial para el metabolismo celular. La angiogénesis en la pulpa es un proceso constante durante la vida del diente. Los indicadores de hipoxia en un tejido, como HIF-1α, así como los marcadores de desestabilización vascular, como A...

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Autores:: Muñoz Alvear, Hernán Dario

Tipo de recurso:: Article of investigation

Fecha de publicación:: 2020

Institución:: Universidad Cooperativa de Colombia

Repositorio:: Repositorio UCC

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repository_id_str
dc.title.spa.fl_str_mv	Expression of early angiogenesis indicators in mature versus immature teeth
title	Expression of early angiogenesis indicators in mature versus immature teeth
spellingShingle	Expression of early angiogenesis indicators in mature versus immature teeth Angiogénesis Pulpa dental humana HIF‐1α ANG1 ANG2 TIE2 Angiogenesis Human dental pulp HIF‐1α ANG1 ANG2 TIE2
title_short	Expression of early angiogenesis indicators in mature versus immature teeth
title_full	Expression of early angiogenesis indicators in mature versus immature teeth
title_fullStr	Expression of early angiogenesis indicators in mature versus immature teeth
title_full_unstemmed	Expression of early angiogenesis indicators in mature versus immature teeth
title_sort	Expression of early angiogenesis indicators in mature versus immature teeth
dc.creator.fl_str_mv	Muñoz Alvear, Hernán Dario
dc.contributor.author.none.fl_str_mv	Muñoz Alvear, Hernán Dario
dc.subject.spa.fl_str_mv	Angiogénesis Pulpa dental humana HIF‐1α ANG1 ANG2 TIE2
topic	Angiogénesis Pulpa dental humana HIF‐1α ANG1 ANG2 TIE2 Angiogenesis Human dental pulp HIF‐1α ANG1 ANG2 TIE2
dc.subject.other.spa.fl_str_mv	Angiogenesis Human dental pulp HIF‐1α ANG1 ANG2 TIE2
description	Antecedentes: El balance adecuado de oxígeno en la pulpa dental es esencial para el metabolismo celular. La angiogénesis en la pulpa es un proceso constante durante la vida del diente. Los indicadores de hipoxia en un tejido, como HIF-1α, así como los marcadores de desestabilización vascular, como ANG2 y su receptor TIE2, son necesarios para la angiogénesis. Por lo tanto, el propósito de este estudio es evaluar la expresión de HIF-1α, ANG1, ANG2 y TIE2 en la pulpa dental como indicadores tempranos de angiogénesis en dientes con desarrollo radicular completo e incompleto. Métodos: Se obtuvieron cuarenta pulpas dentales humanas de terceros molares recién extraídos divididos en dos grupos: desarrollo radicular incompleto (n = 20) y completo (n = 20). Las pulpas dentales se almacenaron a -80 °C, se descongelaron en baño de hielo y se volvieron a congelar con sus respectivos deshielos para desintegrar el tejido. Se realizaron tres ciclos de sonicación hasta homogeneizar los tejidos, luego se descongelaron por centrifugación y se recolectó el sobrenadante para la detección de los marcadores a estudiar. Las muestras se procesaron para la prueba ELISA utilizando el principio ELISA-sándwich. Se realizaron pruebas t de Student y U de Mann-Whitney para determinar diferencias estadísticamente significativas entre los grupos. Resultados: En el grupo de desarrollo radicular completo, las expresiones de HIF-1α, ANG1, ANG2 y TIE2 fueron significativamente mayores que su expresión en el grupo de desarrollo radicular incompleto. Conclusiones: El proceso angiogénico parece ser un proceso fisiológico en la pulpa dental. La actividad angiogénica es mayor en los dientes con ápices maduros que en los inmaduros.
publishDate	2020
dc.date.issued.none.fl_str_mv	2020-11-01
dc.date.accessioned.none.fl_str_mv	2022-10-13T23:38:28Z
dc.date.available.none.fl_str_mv	2022-10-13T23:38:28Z
dc.type.none.fl_str_mv	Artículos Científicos
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dc.identifier.issn.spa.fl_str_mv	1472-6831
dc.identifier.uri.spa.fl_str_mv	https://doi.org/10.1186/s12903-020-01313-1
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/20.500.12494/46726
dc.identifier.bibliographicCitation.spa.fl_str_mv	Caviedes-Bucheli, J., Lopez-Moncayo, L. F., Muñoz-Alvear, H. D., Hernandez-Acosta, F., Pantoja-Mora, M., Rodriguez-Guerrero, A. S., López-Ordoñez, A., Díaz, L. E., Gomez-Sosa, J. F., & Munoz, H. R. (2020). Expression of early angiogenesis indicators in mature versus immature teeth. BMC oral health, 20(1), 324.https://repository.ucc.edu.co/handle/20.500.12494/46726
identifier_str_mv	1472-6831 Caviedes-Bucheli, J., Lopez-Moncayo, L. F., Muñoz-Alvear, H. D., Hernandez-Acosta, F., Pantoja-Mora, M., Rodriguez-Guerrero, A. S., López-Ordoñez, A., Díaz, L. E., Gomez-Sosa, J. F., & Munoz, H. R. (2020). Expression of early angiogenesis indicators in mature versus immature teeth. BMC oral health, 20(1), 324.https://repository.ucc.edu.co/handle/20.500.12494/46726
url	https://doi.org/10.1186/s12903-020-01313-1 https://hdl.handle.net/20.500.12494/46726
dc.relation.isversionof.spa.fl_str_mv	https://bmcoralhealth.biomedcentral.com/articles/10.1186/s12903-020-01313-1
dc.relation.ispartofjournal.spa.fl_str_mv	BMC Oral Health
dc.relation.references.spa.fl_str_mv	Gomez‐Sosa JF, Caviedes‐Bucheli J, Diaz‐Barrera LE, Munoz HR. Gene expression of growth factors with angiogenic potential in human dental pulp tissue from teeth with complete and incomplete root development. Int Endod J. 2019;52:1716–22. Kim HB, Baik KY, Choung PH, Chung JH. Pulse frequency dependency of photobiomodulation on the bioenergetic functions of human dental pulp stem cells. Sci Rep. 2017;7:15927. Ziello JE, Jovin IS, Huang Y. Hypoxia‐Inducible Factor (HIF)‐1 regulatory pathway and its potential for therapeutic intervention in malignancy and ischemia. Yale J Biol Med. 2007;80:51–60. Roberts‐Clark DJ, Smith AJ. Angiogenic growth factors in human dentine matrix. Arch Oral Biol. 2000;45:1013–6. Li J, Parada C, Chai Y. Cellular and molecular mechanisms of tooth root development. Vol. 144, Development 2017; 144: 374–84. Xue C, Shen Y, Li X, Li B, Zhao S, Gu J, Chen Y, Ma B, Wei J, Han Q, Zhao RC. Exosomes derived from hypoxia‐treated human adipose mesenchymal stem cells enhance angiogenesis through the PKA signaling pathway. Stem Cells Dev. 2018;27:456–65. Derringer KA, Linden RWA. Angiogenic growth factors released in human dental pulp following orthodontic force. Arch Oral Biol. 2003;48:285–91. Balamurugan K. HIF‐1 at the crossroads of hypoxia, inflammation, and cancer. Int J Cancer. 2016;138:1058–66. Zhou J, Sun C. SENP1/HIF‐1α axis works in angiogenesis of human dental pulp stem cells. J Biochem Mol Toxicol. 2020;34:e22436. Aranha AMF, Zhang Z, Neiva KG, Costa CAS, Hebling J, Nör JE. Hypoxia enhances the angiogenic potential of human dental pulp cells. J Endod. 2010;36:1633–7. Papetti M, Herman IM. Mechanisms of normal and tumor‐derived angio‐ genesis. Am J Physiol Cell Physiol. 2002;282:C947–70. Caviedes‐Bucheli J, Gomez‐Sosa JF, Azuero‐Holguin MM, Ormeno‐Gomez M, Pinto‐Pascual V, Munoz HR. Angiogenic mechanisms of human dental pulp and their relationship with substance P expression in response to occlusal trauma. Int Endod J. 2017;50:339–51. Grando Mattuella L, de Figueiredo JAP, Nör JE, de Araujo FB, Fossati ACM. Vascular endothelial growth factor receptor‐2 expression in the pulp of human primary and young permanent teeth. J Endod. 2007;33:1408–12. Davis S, Aldrich TH, Jones PF, Acheson A, Compton DL, Jain V, Ryan TE, Bruno J, Radziejewski C, Maisonpierre PC, Yancopoulos GD. Isolation of angiopoietin‐1, a ligand for the TIE2 receptor, by secretion‐trap expres‐ sion cloning. Cell. 1996;87:1161–9. Maisonpierre PC, Suri C, Jones PF, Bartunkova S, Wiegand SJ, Radziejewski C, Compton D, McClain J, Aldrich TH, Papadopoulos N, Daly TJ, Davis S, Sato TN, Yancopoulos GD. Angiopoietin‐2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis. Science. 1997;277:55–60. Murakami M. Signaling required for blood vessel maintenance: molecular basis and pathological manifestations, vol. 2012, Int J Vasc Med 2012; 2012: 293641. Vallée A, Guillevin R, Vallée JN. Vasculogenesis and angiogenesis initiation under normoxic conditions through Wnt/β‐catenin pathway in gliomas. Rev Neurosci. 2017;29:71–91. Michiels C. Physiological and pathological responses to hypoxia. Am J Pathol. 2004;164:1875–82. Sakdee JB, White RR, Pagonis TC, Hauschka PV. Hypoxia‐amplified prolif‐ eration of human dental pulp cells. J Endod. 2009;35:818–23. Wei F, Yang S, Xu H, Guo Q, Li Q, Hu L, Liu D, Wang C. Expression and function of hypoxia inducible factor‐1 α and vascular endothelial growth factor in pulp tissue of teeth under orthodontic movement. Mediators Inflamm. 2015;2015:1–9. Grando Mattuella L, Westphalen Bento L, Poli de Figueiredo JA, Eduardo Nör J, Borba de Araujo F, Medeiros Fossati AC. Vascular endothelial growth factor and its relationship with the dental pulp. J Endod 2007; 33: 524–30.
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dc.publisher.spa.fl_str_mv	Jennifer Harman Universidad Cooperativa de Colombia, Posgrado, Especialización en Endodoncia, Pasto
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spelling	Muñoz Alvear, Hernán Dario202022-10-13T23:38:28Z2022-10-13T23:38:28Z2020-11-011472-6831https://doi.org/10.1186/s12903-020-01313-1https://hdl.handle.net/20.500.12494/46726Caviedes-Bucheli, J., Lopez-Moncayo, L. F., Muñoz-Alvear, H. D., Hernandez-Acosta, F., Pantoja-Mora, M., Rodriguez-Guerrero, A. S., López-Ordoñez, A., Díaz, L. E., Gomez-Sosa, J. F., & Munoz, H. R. (2020). Expression of early angiogenesis indicators in mature versus immature teeth. BMC oral health, 20(1), 324.https://repository.ucc.edu.co/handle/20.500.12494/46726Antecedentes: El balance adecuado de oxígeno en la pulpa dental es esencial para el metabolismo celular. La angiogénesis en la pulpa es un proceso constante durante la vida del diente. Los indicadores de hipoxia en un tejido, como HIF-1α, así como los marcadores de desestabilización vascular, como ANG2 y su receptor TIE2, son necesarios para la angiogénesis. Por lo tanto, el propósito de este estudio es evaluar la expresión de HIF-1α, ANG1, ANG2 y TIE2 en la pulpa dental como indicadores tempranos de angiogénesis en dientes con desarrollo radicular completo e incompleto. Métodos: Se obtuvieron cuarenta pulpas dentales humanas de terceros molares recién extraídos divididos en dos grupos: desarrollo radicular incompleto (n = 20) y completo (n = 20). Las pulpas dentales se almacenaron a -80 °C, se descongelaron en baño de hielo y se volvieron a congelar con sus respectivos deshielos para desintegrar el tejido. Se realizaron tres ciclos de sonicación hasta homogeneizar los tejidos, luego se descongelaron por centrifugación y se recolectó el sobrenadante para la detección de los marcadores a estudiar. Las muestras se procesaron para la prueba ELISA utilizando el principio ELISA-sándwich. Se realizaron pruebas t de Student y U de Mann-Whitney para determinar diferencias estadísticamente significativas entre los grupos. Resultados: En el grupo de desarrollo radicular completo, las expresiones de HIF-1α, ANG1, ANG2 y TIE2 fueron significativamente mayores que su expresión en el grupo de desarrollo radicular incompleto. Conclusiones: El proceso angiogénico parece ser un proceso fisiológico en la pulpa dental. La actividad angiogénica es mayor en los dientes con ápices maduros que en los inmaduros.Background: Proper oxygen balance in the dental pulp is essential for cell metabolism. Angiogenesis in the pulp is a constant process during the life of the tooth. Hypoxia indicators in a tissue, such as HIF‐1α, as well as vascular desta‐ bilization markers, such as ANG2 and its receptor TIE2, are necessary for angiogenesis. Therefore the purpose of this study is to evaluate the expression of HIF‐1α, ANG1, ANG2 and TIE2 in dental pulp as early angiogenesis indicators in teeth with complete and incomplete root development. Methods: Forty human dental pulps were obtained from freshly extracted third molars divided into two groups: incomplete (n = 20) and complete (n = 20) root development. Dental pulps were stored at − 80 °C, defrosted in an ice bath and re‐frozen with their respective thaws to disintegrate the tissue. Three sonication cycles were performed until the tissues were homogenized, then thaw were centrifuged and the supernatant was collected for the detection of the markers to be studied. The samples were processed for the ELISA test using the ELISA‐sandwich principle. Student t and Mann–Whitney U tests were performed to determine statistically significant differences between groups. Results: In the complete root development, HIF‐1α, ANG1, ANG2 and TIE2 expressions were significantly higher than their expression in the incomplete root development group. Conclusions: The angiogenic process seems to be a physiological process in the dental pulp. Angiogenic activity is higher in teeth with mature than immature apex teeth.INTRODUCCION. -- METODOS. -- Preparación de espécimen. -- Prueba de Elisa. -- Análisis. --Estadístico. -- RESULTADOS. -- DISCUSION. -- CONCLUSION.https://scienti.minciencias.gov.co/cvlac/EnRecursoHumano/inicio.dohttps://orcid.org/0000-0002-4327-4242https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000002911hernan.munoz@campusucc.edu.co6Jennifer HarmanUniversidad Cooperativa de Colombia, Posgrado, Especialización en Endodoncia, PastoEspecialización en endodonciaPastohttps://bmcoralhealth.biomedcentral.com/articles/10.1186/s12903-020-01313-1BMC Oral HealthGomez‐Sosa JF, Caviedes‐Bucheli J, Diaz‐Barrera LE, Munoz HR. Gene expression of growth factors with angiogenic potential in human dental pulp tissue from teeth with complete and incomplete root development. Int Endod J. 2019;52:1716–22.Kim HB, Baik KY, Choung PH, Chung JH. Pulse frequency dependency of photobiomodulation on the bioenergetic functions of human dental pulp stem cells. Sci Rep. 2017;7:15927.Ziello JE, Jovin IS, Huang Y. Hypoxia‐Inducible Factor (HIF)‐1 regulatory pathway and its potential for therapeutic intervention in malignancy and ischemia. Yale J Biol Med. 2007;80:51–60.Roberts‐Clark DJ, Smith AJ. Angiogenic growth factors in human dentine matrix. Arch Oral Biol. 2000;45:1013–6.Li J, Parada C, Chai Y. Cellular and molecular mechanisms of tooth root development. Vol. 144, Development 2017; 144: 374–84.Xue C, Shen Y, Li X, Li B, Zhao S, Gu J, Chen Y, Ma B, Wei J, Han Q, Zhao RC. Exosomes derived from hypoxia‐treated human adipose mesenchymal stem cells enhance angiogenesis through the PKA signaling pathway. Stem Cells Dev. 2018;27:456–65.Derringer KA, Linden RWA. Angiogenic growth factors released in human dental pulp following orthodontic force. Arch Oral Biol. 2003;48:285–91.Balamurugan K. HIF‐1 at the crossroads of hypoxia, inflammation, and cancer. Int J Cancer. 2016;138:1058–66.Zhou J, Sun C. SENP1/HIF‐1α axis works in angiogenesis of human dental pulp stem cells. J Biochem Mol Toxicol. 2020;34:e22436.Aranha AMF, Zhang Z, Neiva KG, Costa CAS, Hebling J, Nör JE. Hypoxia enhances the angiogenic potential of human dental pulp cells. J Endod. 2010;36:1633–7.Papetti M, Herman IM. Mechanisms of normal and tumor‐derived angio‐ genesis. Am J Physiol Cell Physiol. 2002;282:C947–70.Caviedes‐Bucheli J, Gomez‐Sosa JF, Azuero‐Holguin MM, Ormeno‐Gomez M, Pinto‐Pascual V, Munoz HR. Angiogenic mechanisms of human dental pulp and their relationship with substance P expression in response to occlusal trauma. Int Endod J. 2017;50:339–51.Grando Mattuella L, de Figueiredo JAP, Nör JE, de Araujo FB, Fossati ACM. Vascular endothelial growth factor receptor‐2 expression in the pulp of human primary and young permanent teeth. J Endod. 2007;33:1408–12.Davis S, Aldrich TH, Jones PF, Acheson A, Compton DL, Jain V, Ryan TE, Bruno J, Radziejewski C, Maisonpierre PC, Yancopoulos GD. Isolation of angiopoietin‐1, a ligand for the TIE2 receptor, by secretion‐trap expres‐ sion cloning. Cell. 1996;87:1161–9.Maisonpierre PC, Suri C, Jones PF, Bartunkova S, Wiegand SJ, Radziejewski C, Compton D, McClain J, Aldrich TH, Papadopoulos N, Daly TJ, Davis S, Sato TN, Yancopoulos GD. Angiopoietin‐2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis. Science. 1997;277:55–60.Murakami M. Signaling required for blood vessel maintenance: molecular basis and pathological manifestations, vol. 2012, Int J Vasc Med 2012; 2012: 293641.Vallée A, Guillevin R, Vallée JN. Vasculogenesis and angiogenesis initiation under normoxic conditions through Wnt/β‐catenin pathway in gliomas. Rev Neurosci. 2017;29:71–91.Michiels C. Physiological and pathological responses to hypoxia. Am J Pathol. 2004;164:1875–82.Sakdee JB, White RR, Pagonis TC, Hauschka PV. Hypoxia‐amplified prolif‐ eration of human dental pulp cells. J Endod. 2009;35:818–23.Wei F, Yang S, Xu H, Guo Q, Li Q, Hu L, Liu D, Wang C. Expression and function of hypoxia inducible factor‐1 α and vascular endothelial growth factor in pulp tissue of teeth under orthodontic movement. Mediators Inflamm. 2015;2015:1–9.Grando Mattuella L, Westphalen Bento L, Poli de Figueiredo JA, Eduardo Nör J, Borba de Araujo F, Medeiros Fossati AC. Vascular endothelial growth factor and its relationship with the dental pulp. J Endod 2007; 33: 524–30.AngiogénesisPulpa dental humanaHIF‐1αANG1ANG2TIE2AngiogenesisHuman dental pulpHIF‐1αANG1ANG2TIE2Expression of early angiogenesis indicators in mature versus immature teethArtículos Científicoshttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribución – Compartir igualinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationORIGINAL2022-caviedesbuchelijavermunozalvearhernandariolopezmoncayoluis fernando-Expression_of_early_angiogenesis_indicators_in_mature_versus_immature_teeth-FormatoPublicacionWeb .pdf2022-caviedesbuchelijavermunozalvearhernandariolopezmoncayoluis fernando-Expression_of_early_angiogenesis_indicators_in_mature_versus_immature_teeth-FormatoPublicacionWeb .pdfArticuloapplication/pdf861191https://repository.ucc.edu.co/bitstreams/ceded3ec-4094-4475-b8b6-ad788efb3428/download250d8d584c8fc597f2ddf7bcec182275MD532022-caviedesbuchelijavermunozalvearhernandariolopezmoncayoluis fernando-Expression_of_early_angiogenesis_indicators_in_mature_versus_immature_teeth-licenciadeuso.pdf2022-caviedesbuchelijavermunozalvearhernandariolopezmoncayoluis fernando-Expression_of_early_angiogenesis_indicators_in_mature_versus_immature_teeth-licenciadeuso.pdfLicencia de usoapplication/pdf212801https://repository.ucc.edu.co/bitstreams/43d3aed7-5476-48ff-9a58-11a99f0b3f27/downloade7c082fe8ee974b31d968c28e8e495e2MD54LICENSElicense.txtlicense.txttext/plain; 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Expression of early angiogenesis indicators in mature versus immature teeth

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