An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses
The real-time reverse transcription-polymerase chain reaction (real-time RT-qPCR) has become a leading technique for the detection and quantification of arboviruses, including Chikungunya, Dengue, and Zika viruses. In this study, an updated real-time RT-qPCR assay was designed and evaluated together...
- Autores:
-
Álvarez Díaz, Diego Alejandro
Valencia Álvarez, Emmanuel
Rivera, Jorge Alonso
Rengifo, Aura Caterine
Usme Ciro, José Aldemar
Peláez Carvajal, Dioselina
Lozano Jimenez, Yenny Y.
Torres Fernández, Orlando
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2021
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/46183
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/46183
- Palabra clave:
- Arbovirus
Carga viral
RT-qPCR
Chikungunya
Dengue
Zika
Arbovirus
Viral load
RT-qPCR
Chikungunya
Dengue
Zika
- Rights
- openAccess
- License
- Atribución
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| dc.title.spa.fl_str_mv |
An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses |
| title |
An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses |
| spellingShingle |
An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses Arbovirus Carga viral RT-qPCR Chikungunya Dengue Zika Arbovirus Viral load RT-qPCR Chikungunya Dengue Zika |
| title_short |
An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses |
| title_full |
An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses |
| title_fullStr |
An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses |
| title_full_unstemmed |
An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses |
| title_sort |
An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses |
| dc.creator.fl_str_mv |
Álvarez Díaz, Diego Alejandro Valencia Álvarez, Emmanuel Rivera, Jorge Alonso Rengifo, Aura Caterine Usme Ciro, José Aldemar Peláez Carvajal, Dioselina Lozano Jimenez, Yenny Y. Torres Fernández, Orlando |
| dc.contributor.author.none.fl_str_mv |
Álvarez Díaz, Diego Alejandro Valencia Álvarez, Emmanuel Rivera, Jorge Alonso Rengifo, Aura Caterine Usme Ciro, José Aldemar Peláez Carvajal, Dioselina Lozano Jimenez, Yenny Y. Torres Fernández, Orlando |
| dc.subject.spa.fl_str_mv |
Arbovirus Carga viral RT-qPCR Chikungunya Dengue Zika |
| topic |
Arbovirus Carga viral RT-qPCR Chikungunya Dengue Zika Arbovirus Viral load RT-qPCR Chikungunya Dengue Zika |
| dc.subject.other.spa.fl_str_mv |
Arbovirus Viral load RT-qPCR Chikungunya Dengue Zika |
| description |
The real-time reverse transcription-polymerase chain reaction (real-time RT-qPCR) has become a leading technique for the detection and quantification of arboviruses, including Chikungunya, Dengue, and Zika viruses. In this study, an updated real-time RT-qPCR assay was designed and evaluated together with a synthetic positive-control chimeric RNA for the simultaneous detection and quantification of Chikungunya, Dengue, and Zika viruses. Amplification assays were performed to verify the construct integrity and optimal reaction/thermal cycling conditions. The analytical sensitivity of the assay was determined for each virus in single and multiplex reactions, as well as the performance in the detection and viral load quantification of experimental samples. The real-time RT-qPCR assay presented here allowed for the simultaneous detection and quantification of Chikungunya, Dengue, and Zika viruses and could be applied in several studies where the accurate quantification of viral genomes is required. |
| publishDate |
2021 |
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2021-06-09 |
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2022-08-19T23:50:49Z |
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2022-08-19T23:50:49Z |
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Artículos Científicos |
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1567-1348 |
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10.1016/j.meegid.2021.104967 |
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https://hdl.handle.net/20.500.12494/46183 |
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Álvarez-Díaz DA, Valencia-Álvarez E, Rivera JA, Rengifo AC, Usme-Ciro JA, et al. (2021). An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses. Infect Genet Evol. 2021 Jun 9;93:104967. doi: 10.1016/j.meegid.2021.104967.https://repository.ucc.edu.co/handle/20.500.12494/46183 |
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1567-1348 10.1016/j.meegid.2021.104967 Álvarez-Díaz DA, Valencia-Álvarez E, Rivera JA, Rengifo AC, Usme-Ciro JA, et al. (2021). An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses. Infect Genet Evol. 2021 Jun 9;93:104967. doi: 10.1016/j.meegid.2021.104967.https://repository.ucc.edu.co/handle/20.500.12494/46183 |
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https://hdl.handle.net/20.500.12494/46183 |
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https://www.sciencedirect.com/science/article/abs/pii/S1567134821002641?via%3Dihub |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Infection, Genetics and Evolution |
| dc.relation.references.spa.fl_str_mv |
Alvarez-Diaz, D.A., Quintero, P.A., Pelaez-Carvajal, D., Ajami, N.J., Usme-Ciro, J.A., 2019. Novel pan-serotype control RNA for dengue virus typing through real-time reverse transcription-polymerase chain reaction. J. Virol. Methods 271, 113677. Amraoui, F., Failloux, A.B., 2016. Chikungunya: an unexpected emergence in Europe. Curr. Opin. Virol. 21, 146–150. Angelo, J.R., Fuller, T.L., Leandro, B.B.S., Praca, H.L.F., Marques, R.D., Ferreira, J.M.C., Pupe, C.C.B., Perez, O.C., Nielsen-Saines, K., Nascimento, O.J.M., Sabroza, P.C., 2020. Neurological complications associated with emerging viruses in Brazil. Int. J. Gynaecol. Obstet. 148 (Suppl. 2), 70–75. Boga, J.A., Alvarez-Arguelles, M.E., Rojo-Alba, S., Rodriguez, M., de Ona, M., Melon, S., 2019. Simultaneous detection of dengue virus, Chikungunya virus, Zika virus, yellow fever virus and West Nile virus. J. Virol. Methods 268, 53–55. Chen, H., Parimelalagan, M., Lai, Y.L., Lee, K.S., Koay, E.S., Hapuarachchi, H.C., Ng, L.C., Ho, P.S., Chu, J.J., 2015. Development and evaluation of a SYBR green-based realtime multiplex RT-PCR assay for simultaneous detection and serotyping of dengue and Chikungunya viruses. J. Mol. Diagn. 17, 722–728. Colombo, T.E., Versiani, A.F., Dutra, K.R., Rubiato, J.G.D., Galvao, T.M., Negri Reis, A.F., Nogueira, M.L., 2019. Performance of CDC Trioplex qPCR during a dengue outbreak in Brazil. J. Clin. Virol. 121, 104208. Elnifro, E.M., Ashshi, A.M., Cooper, R.J., Klapper, P.E., 2000. Multiplex PCR: optimization and application in diagnostic virology. Clin. Microbiol. Rev. 13, 559–570. Johnson, B.W., Russell, B.J., Lanciotti, R.S., 2005. Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay. J. Clin. Microbiol. 43 (10), 4977–4983. https://doi.org/10.1128/JCM.43.10.4977-4983.2005. Kang, T., 2019. Basic principles for developing real-time PCR methods used in food analysis: a review. Trends Food Sci. Technol. 91, 574–585. Kikuti, M., Tauro, L.B., Moreira, P.S.S., Nascimento, L.C.J., Portilho, M.M., Soares, G.C., Weaver, S.C., Reis, M.G., Kitron, U., Ribeiro, G.S., 2020. Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses. Int. J. Infect. Dis. 91, 38–43. Kuhlisch, C., Kampen, H., Walther, D., 2018. The Asian tiger mosquito Aedes albopictus (Diptera: Culicidae) in Central Germany: surveillance in its northernmost distribution area. Acta Trop. 188, 78–85. Laamiri, N., Aouini, R., Boutheina, M., Abdeljelil, G., Issam, H., 2017. A multiplex realtime RT-PCR for simultaneous detection of four most common avian respiratory viruses. Virology 515, 29–37. Lima, M.E.S., Bachur, T.P.R., Aragao, G.F., 2019. Guillain-Barre syndrome and its correlation with dengue, Zika and chikungunya viruses infection based on a literature review of reported cases in Brazil. Acta Trop. 197, 105064. Mansuy, J.M., Lhomme, S., Cazabat, M., Pasquier, C., Martin-Blondel, G., Izopet, J., 2018. Detection of Zika, dengue and chikungunya viruses using single-reaction multiplex real-time RT-PCR. Diagn. Microbiol. Infect. Dis. 92, 284–287. Martinez, J.D., Garza, J.A.C., Cuellar-Barboza, A., 2019. Going Viral 2019: Zika, Chikungunya, and Dengue. Dermatol. Clin. 37, 95–105. |
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Michel Tibayrenc Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa Marta |
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Medicina |
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Santa Marta |
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Universidad Cooperativa de Colombia |
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Álvarez Díaz, Diego AlejandroValencia Álvarez, EmmanuelRivera, Jorge AlonsoRengifo, Aura CaterineUsme Ciro, José AldemarPeláez Carvajal, DioselinaLozano Jimenez, Yenny Y.Torres Fernández, Orlando932022-08-19T23:50:49Z2022-08-19T23:50:49Z2021-06-091567-134810.1016/j.meegid.2021.104967https://hdl.handle.net/20.500.12494/46183Álvarez-Díaz DA, Valencia-Álvarez E, Rivera JA, Rengifo AC, Usme-Ciro JA, et al. (2021). An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses. Infect Genet Evol. 2021 Jun 9;93:104967. doi: 10.1016/j.meegid.2021.104967.https://repository.ucc.edu.co/handle/20.500.12494/46183The real-time reverse transcription-polymerase chain reaction (real-time RT-qPCR) has become a leading technique for the detection and quantification of arboviruses, including Chikungunya, Dengue, and Zika viruses. In this study, an updated real-time RT-qPCR assay was designed and evaluated together with a synthetic positive-control chimeric RNA for the simultaneous detection and quantification of Chikungunya, Dengue, and Zika viruses. Amplification assays were performed to verify the construct integrity and optimal reaction/thermal cycling conditions. The analytical sensitivity of the assay was determined for each virus in single and multiplex reactions, as well as the performance in the detection and viral load quantification of experimental samples. The real-time RT-qPCR assay presented here allowed for the simultaneous detection and quantification of Chikungunya, Dengue, and Zika viruses and could be applied in several studies where the accurate quantification of viral genomes is required.https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000318507https://orcid.org/0000-0002-8093-0544https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000008981jose.usmec@campusucc.edu.cohttps://scholar.google.com.co/citations?user=cU2KyT4AAAAJ&hl=en8Michel TibayrencUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa MartaMedicinaSanta Martahttps://www.sciencedirect.com/science/article/abs/pii/S1567134821002641?via%3DihubInfection, Genetics and EvolutionAlvarez-Diaz, D.A., Quintero, P.A., Pelaez-Carvajal, D., Ajami, N.J., Usme-Ciro, J.A., 2019. Novel pan-serotype control RNA for dengue virus typing through real-time reverse transcription-polymerase chain reaction. J. Virol. Methods 271, 113677.Amraoui, F., Failloux, A.B., 2016. Chikungunya: an unexpected emergence in Europe. Curr. Opin. Virol. 21, 146–150.Angelo, J.R., Fuller, T.L., Leandro, B.B.S., Praca, H.L.F., Marques, R.D., Ferreira, J.M.C., Pupe, C.C.B., Perez, O.C., Nielsen-Saines, K., Nascimento, O.J.M., Sabroza, P.C., 2020. Neurological complications associated with emerging viruses in Brazil. Int. J. Gynaecol. Obstet. 148 (Suppl. 2), 70–75.Boga, J.A., Alvarez-Arguelles, M.E., Rojo-Alba, S., Rodriguez, M., de Ona, M., Melon, S., 2019. Simultaneous detection of dengue virus, Chikungunya virus, Zika virus, yellow fever virus and West Nile virus. J. Virol. Methods 268, 53–55.Chen, H., Parimelalagan, M., Lai, Y.L., Lee, K.S., Koay, E.S., Hapuarachchi, H.C., Ng, L.C., Ho, P.S., Chu, J.J., 2015. Development and evaluation of a SYBR green-based realtime multiplex RT-PCR assay for simultaneous detection and serotyping of dengue and Chikungunya viruses. J. Mol. Diagn. 17, 722–728.Colombo, T.E., Versiani, A.F., Dutra, K.R., Rubiato, J.G.D., Galvao, T.M., Negri Reis, A.F., Nogueira, M.L., 2019. Performance of CDC Trioplex qPCR during a dengue outbreak in Brazil. J. Clin. Virol. 121, 104208.Elnifro, E.M., Ashshi, A.M., Cooper, R.J., Klapper, P.E., 2000. Multiplex PCR: optimization and application in diagnostic virology. Clin. Microbiol. Rev. 13, 559–570.Johnson, B.W., Russell, B.J., Lanciotti, R.S., 2005. Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay. J. Clin. Microbiol. 43 (10), 4977–4983. https://doi.org/10.1128/JCM.43.10.4977-4983.2005.Kang, T., 2019. Basic principles for developing real-time PCR methods used in food analysis: a review. Trends Food Sci. Technol. 91, 574–585.Kikuti, M., Tauro, L.B., Moreira, P.S.S., Nascimento, L.C.J., Portilho, M.M., Soares, G.C., Weaver, S.C., Reis, M.G., Kitron, U., Ribeiro, G.S., 2020. Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses. Int. J. Infect. Dis. 91, 38–43.Kuhlisch, C., Kampen, H., Walther, D., 2018. The Asian tiger mosquito Aedes albopictus (Diptera: Culicidae) in Central Germany: surveillance in its northernmost distribution area. Acta Trop. 188, 78–85.Laamiri, N., Aouini, R., Boutheina, M., Abdeljelil, G., Issam, H., 2017. A multiplex realtime RT-PCR for simultaneous detection of four most common avian respiratory viruses. Virology 515, 29–37.Lima, M.E.S., Bachur, T.P.R., Aragao, G.F., 2019. Guillain-Barre syndrome and its correlation with dengue, Zika and chikungunya viruses infection based on a literature review of reported cases in Brazil. Acta Trop. 197, 105064.Mansuy, J.M., Lhomme, S., Cazabat, M., Pasquier, C., Martin-Blondel, G., Izopet, J., 2018. Detection of Zika, dengue and chikungunya viruses using single-reaction multiplex real-time RT-PCR. Diagn. Microbiol. Infect. Dis. 92, 284–287.Martinez, J.D., Garza, J.A.C., Cuellar-Barboza, A., 2019. Going Viral 2019: Zika, Chikungunya, and Dengue. Dermatol. Clin. 37, 95–105.ArbovirusCarga viralRT-qPCRChikungunyaDengueZikaArbovirusViral loadRT-qPCRChikungunyaDengueZikaAn updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika virusesArtículos Científicoshttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribucióninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repository.ucc.edu.co/bitstreams/e0970c0e-0da9-498a-be1c-1c9d270de925/download8a4605be74aa9ea9d79846c1fba20a33MD54ORIGINAL34. An updated RT-qPCR assay for the symultaneous detection CHIKV-DENV-ZIKV. Alvarez-Diaz et al 2021.pdf34. An updated RT-qPCR assay for the symultaneous detection CHIKV-DENV-ZIKV. 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