Curcumin inhibits in vitro SARS-CoV-2 infection in Vero E6 cells through multiple antiviral mechanisms
Due to the scarcity of therapeutic approaches for COVID-19, we investigated the antiviral and anti-inflammatory properties of curcumin against SARS-CoV-2 using in vitro models. The cytotoxicity of curcumin was evaluated using MTT assay in Vero E6 cells. The antiviral activity of this compound agains...
- Autores:
-
Marín Palma, Damariz
Tabares Guevara, Jorge
Zapata Cardona, María Isabel
Flórez Álvarez, Lizdany
Yepes, Lina M.
Rugeles López, María Teresa
Zapata Builes, Wildeman
Hernández López, Juan Carlos
Taborda, Natalia Andrea
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2021
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/44426
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/44426
- Palabra clave:
- Curcumin
Antiviral
COVID-19
SARS-CoV-2
Immune response
Inflammation
D614G strain
Delta variant
- Rights
- openAccess
- License
- Atribución
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| dc.title.spa.fl_str_mv |
Curcumin inhibits in vitro SARS-CoV-2 infection in Vero E6 cells through multiple antiviral mechanisms |
| title |
Curcumin inhibits in vitro SARS-CoV-2 infection in Vero E6 cells through multiple antiviral mechanisms |
| spellingShingle |
Curcumin inhibits in vitro SARS-CoV-2 infection in Vero E6 cells through multiple antiviral mechanisms Curcumin Antiviral COVID-19 SARS-CoV-2 Immune response Inflammation D614G strain Delta variant |
| title_short |
Curcumin inhibits in vitro SARS-CoV-2 infection in Vero E6 cells through multiple antiviral mechanisms |
| title_full |
Curcumin inhibits in vitro SARS-CoV-2 infection in Vero E6 cells through multiple antiviral mechanisms |
| title_fullStr |
Curcumin inhibits in vitro SARS-CoV-2 infection in Vero E6 cells through multiple antiviral mechanisms |
| title_full_unstemmed |
Curcumin inhibits in vitro SARS-CoV-2 infection in Vero E6 cells through multiple antiviral mechanisms |
| title_sort |
Curcumin inhibits in vitro SARS-CoV-2 infection in Vero E6 cells through multiple antiviral mechanisms |
| dc.creator.fl_str_mv |
Marín Palma, Damariz Tabares Guevara, Jorge Zapata Cardona, María Isabel Flórez Álvarez, Lizdany Yepes, Lina M. Rugeles López, María Teresa Zapata Builes, Wildeman Hernández López, Juan Carlos Taborda, Natalia Andrea |
| dc.contributor.author.none.fl_str_mv |
Marín Palma, Damariz Tabares Guevara, Jorge Zapata Cardona, María Isabel Flórez Álvarez, Lizdany Yepes, Lina M. Rugeles López, María Teresa Zapata Builes, Wildeman Hernández López, Juan Carlos Taborda, Natalia Andrea |
| dc.subject.spa.fl_str_mv |
Curcumin Antiviral COVID-19 SARS-CoV-2 Immune response Inflammation D614G strain Delta variant |
| topic |
Curcumin Antiviral COVID-19 SARS-CoV-2 Immune response Inflammation D614G strain Delta variant |
| description |
Due to the scarcity of therapeutic approaches for COVID-19, we investigated the antiviral and anti-inflammatory properties of curcumin against SARS-CoV-2 using in vitro models. The cytotoxicity of curcumin was evaluated using MTT assay in Vero E6 cells. The antiviral activity of this compound against SARS-CoV-2 was evaluated using four treatment strategies (i. pre–post infection treatment, ii. co-treatment, iii. pre-infection, and iv. post-infection). The D614G strain and Delta variant of SARS-CoV-2 were used, and the viral titer was quantified by plaque assay. The anti-inflammatory effect was evaluated in peripheral blood mononuclear cells (PBMCs) using qPCR and ELISA. By pre–post infection treatment, Curcumin (10 µg/mL) exhibited antiviral effect of 99% and 99.8% against DG614 strain and Delta variant, respectively. Curcumin also inhibited D614G strain by pre-infection and post-infection treatment. In addition, curcumin showed a virucidal effect against D614G strain and Delta variant. Finally, the pro-inflammatory cytokines (IL-1β, IL-6, and IL-8) released by PBMCs triggered by SARS-CoV-2 were decreased after treatment with curcumin. Our results suggest that curcumin affects the SARS-CoV-2 replicative cycle and exhibits virucidal effect with a variant/strain independent antiviral effect and immune-modulatory properties. This is the first study that showed a combined (antiviral/anti-inflammatory) effect of curcumin during SARS-CoV-2 infection. However, additional studies are required to define its use as a treatment for the COVID-19. |
| publishDate |
2021 |
| dc.date.issued.none.fl_str_mv |
2021-11-16 |
| dc.date.accessioned.none.fl_str_mv |
2022-03-16T19:46:20Z |
| dc.date.available.none.fl_str_mv |
2022-03-16T19:46:20Z |
| dc.type.none.fl_str_mv |
Artículos Científicos |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.coarversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.uri.spa.fl_str_mv |
10.3390/molecules26226900 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12494/44426 |
| dc.identifier.bibliographicCitation.spa.fl_str_mv |
Marín-Palma D, Tabares-Guevara JH, Zapata-Cardona MI, Flórez-Álvarez L, Yepes LM, Rugeles MT, Zapata-Builes W, Hernandez JC, Taborda NA. (2021) Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms. Molecules. 2021 Nov 16;26(22):6900. doi: 10.3390/molecules26226900. http://hdl.handle.net/20.500.12494/44426 |
| identifier_str_mv |
10.3390/molecules26226900 Marín-Palma D, Tabares-Guevara JH, Zapata-Cardona MI, Flórez-Álvarez L, Yepes LM, Rugeles MT, Zapata-Builes W, Hernandez JC, Taborda NA. (2021) Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms. Molecules. 2021 Nov 16;26(22):6900. doi: 10.3390/molecules26226900. http://hdl.handle.net/20.500.12494/44426 |
| url |
https://hdl.handle.net/20.500.12494/44426 |
| dc.relation.isversionof.spa.fl_str_mv |
https://www.mdpi.com/1420-3049/26/22/6900 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Molecules |
| dc.relation.references.spa.fl_str_mv |
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Marín Palma, DamarizTabares Guevara, JorgeZapata Cardona, María IsabelFlórez Álvarez, LizdanyYepes, Lina M.Rugeles López, María TeresaZapata Builes, WildemanHernández López, Juan CarlosTaborda, Natalia Andrea16;262022-03-16T19:46:20Z2022-03-16T19:46:20Z2021-11-1610.3390/molecules26226900https://hdl.handle.net/20.500.12494/44426Marín-Palma D, Tabares-Guevara JH, Zapata-Cardona MI, Flórez-Álvarez L, Yepes LM, Rugeles MT, Zapata-Builes W, Hernandez JC, Taborda NA. (2021) Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms. Molecules. 2021 Nov 16;26(22):6900. doi: 10.3390/molecules26226900. http://hdl.handle.net/20.500.12494/44426Due to the scarcity of therapeutic approaches for COVID-19, we investigated the antiviral and anti-inflammatory properties of curcumin against SARS-CoV-2 using in vitro models. The cytotoxicity of curcumin was evaluated using MTT assay in Vero E6 cells. The antiviral activity of this compound against SARS-CoV-2 was evaluated using four treatment strategies (i. pre–post infection treatment, ii. co-treatment, iii. pre-infection, and iv. post-infection). The D614G strain and Delta variant of SARS-CoV-2 were used, and the viral titer was quantified by plaque assay. The anti-inflammatory effect was evaluated in peripheral blood mononuclear cells (PBMCs) using qPCR and ELISA. By pre–post infection treatment, Curcumin (10 µg/mL) exhibited antiviral effect of 99% and 99.8% against DG614 strain and Delta variant, respectively. Curcumin also inhibited D614G strain by pre-infection and post-infection treatment. In addition, curcumin showed a virucidal effect against D614G strain and Delta variant. Finally, the pro-inflammatory cytokines (IL-1β, IL-6, and IL-8) released by PBMCs triggered by SARS-CoV-2 were decreased after treatment with curcumin. Our results suggest that curcumin affects the SARS-CoV-2 replicative cycle and exhibits virucidal effect with a variant/strain independent antiviral effect and immune-modulatory properties. This is the first study that showed a combined (antiviral/anti-inflammatory) effect of curcumin during SARS-CoV-2 infection. However, additional studies are required to define its use as a treatment for the COVID-19.https://scienti.colciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000283088http://orcid.org/0000-0002-9200-5698https://scienti.colciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000011355juanc.hernandezl@campusucc.edu.co1-17Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Medellín y EnvigadoMedicinaMedellínhttps://www.mdpi.com/1420-3049/26/22/6900MoleculesZhou, P.; Yang, X.L.; Wang, X.G.; Hu, B.; Zhang, L.; Zhang, W.; Si, H.R.; Zhu, Y.; Li, B.; Huang, C.L.; et al. 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Antiviral activities of 2,6-diaminopurine-based acyclic nucleoside phosphonates against herpesviruses: In vitro study results with pseudorabies virus (PrV, SuHV-1). Vet. Microbiol. 2016, 184, 84–93.Zapata-Cardona, M.I.; Flórez-Álvarez, L.; Zapata-Builes, W.; Guerra-Sandoval, A.L.; Guerra-Almonacid, C.M.; Hincapié-García, J.; Rugeles, M.T.; Hernandez, J.C. Atorvastatin effectively inhibits late replicative cycle steps of SARS-CoV-2 in vitro. bioRxiv 2021.Uzunova, K.; Filipova, E.; Pavlova, V.; Vekov, T. Insights into antiviral mechanisms of remdesivir, lopinavir/ritonavir and chloroquine/hydroxychloroquine affecting the new SARS-CoV-2. Biomed. Pharm. 2020, 131, 110668. [Tandon, R.; Sharp, J.S.; Zhang, F.; Pomin, V.H.; Ashpole, N.M.; Mitra, D.; McCandless, M.G.; Jin, W.; Liu, H.; Sharma, P.; et al. Effective Inhibition of SARS-CoV-2 Entry by Heparin and Enoxaparin Derivatives. J. Virol. 2021, 95Marin-Palma, D.; Castro, G.A.; Cardona-Arias, J.A.; Urcuqui-Inchima, S.; Hernandez, J.C. Lower High-Density Lipoproteins Levels During Human Immunodeficiency Virus Type 1 Infection Are Associated with Increased Inflammatory Markers and Disease Progression. Front Immunol. 2018, 9, 1350Marin-Palma, D.; Sirois, C.M.; Urcuqui-Inchima, S.; Hernandez, J.C. Inflammatory status and severity of disease in dengue patients are associated with lipoprotein alterations. PLoS ONE 2019, 14, e0214245. [Feria-Garzon, M.G.; Rugeles, M.T.; Hernandez, J.C.; Lujan, J.A.; Taborda, N.A. Sulfasalazine as an Immunomodulator of the Inflammatory Process during HIV-1 Infection. Int. J. Mol. 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