Variabilidad genética en regiones codificantes del antígeno de superficie y dominio transcriptasa inversa de la polimerasa del virus de la hepatitis B, Colombia, 2002-2014
Introducción. Se estima que 240 millones de personas en el mundo tienen infección crónica con el virus de la hepatitis B (HBV). En Colombia, la endemia es variable y circulan diferentes genotipos virales. Las mutaciones a lo largo del genoma se han asociado con resistencia antiviral, el escape ante...
- Autores:
-
Peláez Carvajal, Dioselina
Forero, Nidia J.
Escalante Mora, Martha
Laiton Donato, Katherine
Usme Ciro, José Aldemar
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/32708
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/32708
- Palabra clave:
- Virus de la hepatitis B
Genotipo
ADN Polimerasa dirigida por ARN
Mutación
Hepatitis B virus
Genotype
Mutation
RNA Dependent DNA Polymerase
- Rights
- openAccess
- License
- Atribución
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| dc.title.spa.fl_str_mv |
Variabilidad genética en regiones codificantes del antígeno de superficie y dominio transcriptasa inversa de la polimerasa del virus de la hepatitis B, Colombia, 2002-2014 |
| title |
Variabilidad genética en regiones codificantes del antígeno de superficie y dominio transcriptasa inversa de la polimerasa del virus de la hepatitis B, Colombia, 2002-2014 |
| spellingShingle |
Variabilidad genética en regiones codificantes del antígeno de superficie y dominio transcriptasa inversa de la polimerasa del virus de la hepatitis B, Colombia, 2002-2014 Virus de la hepatitis B Genotipo ADN Polimerasa dirigida por ARN Mutación Hepatitis B virus Genotype Mutation RNA Dependent DNA Polymerase |
| title_short |
Variabilidad genética en regiones codificantes del antígeno de superficie y dominio transcriptasa inversa de la polimerasa del virus de la hepatitis B, Colombia, 2002-2014 |
| title_full |
Variabilidad genética en regiones codificantes del antígeno de superficie y dominio transcriptasa inversa de la polimerasa del virus de la hepatitis B, Colombia, 2002-2014 |
| title_fullStr |
Variabilidad genética en regiones codificantes del antígeno de superficie y dominio transcriptasa inversa de la polimerasa del virus de la hepatitis B, Colombia, 2002-2014 |
| title_full_unstemmed |
Variabilidad genética en regiones codificantes del antígeno de superficie y dominio transcriptasa inversa de la polimerasa del virus de la hepatitis B, Colombia, 2002-2014 |
| title_sort |
Variabilidad genética en regiones codificantes del antígeno de superficie y dominio transcriptasa inversa de la polimerasa del virus de la hepatitis B, Colombia, 2002-2014 |
| dc.creator.fl_str_mv |
Peláez Carvajal, Dioselina Forero, Nidia J. Escalante Mora, Martha Laiton Donato, Katherine Usme Ciro, José Aldemar |
| dc.contributor.author.none.fl_str_mv |
Peláez Carvajal, Dioselina Forero, Nidia J. Escalante Mora, Martha Laiton Donato, Katherine Usme Ciro, José Aldemar |
| dc.subject.spa.fl_str_mv |
Virus de la hepatitis B Genotipo ADN Polimerasa dirigida por ARN Mutación |
| topic |
Virus de la hepatitis B Genotipo ADN Polimerasa dirigida por ARN Mutación Hepatitis B virus Genotype Mutation RNA Dependent DNA Polymerase |
| dc.subject.other.spa.fl_str_mv |
Hepatitis B virus Genotype Mutation RNA Dependent DNA Polymerase |
| description |
Introducción. Se estima que 240 millones de personas en el mundo tienen infección crónica con el virus de la hepatitis B (HBV). En Colombia, la endemia es variable y circulan diferentes genotipos virales. Las mutaciones a lo largo del genoma se han asociado con resistencia antiviral, el escape ante la reacción de anticuerpos neutralizadores tras la vacunación o a la infección natural, la infección oculta y la progresión a carcinoma hepatocelular. Objetivo. Identificar los genotipos y las mutaciones presentes en la región codificante del antígeno de superficie (S) y del dominio de la transcriptasa inversa (reverse transcriptase, RT) de la polimerasa del HBV en muestras de suero remitidas al Instituto Nacional de Salud de Colombia para el diagnóstico de hepatitis B, entre el 2002 y el 2014. Materiales y métodos. En 495 muestras de suero positivas para el antígeno de superficie de la hepatitis B (HBsAg) se buscó el ADN viral, se amplificó y secuenció un fragmento de 1.591 nucleótidos y, posteriormente, se hizo el análisis filogenético correspondiente. Resultados. En 66 de las muestras se logró detectar el genoma viral y 28 de ellas se secuenciaron exitosamente. El análisis filogenético permitió identificar los genotipos y subgenotipos F3 y A2. Una muestra presentó simultáneamente las sustituciones de resistencia L180M y M204V, otra presentó la sustitución I169L y en una se identificó la mutación P120Q, previamente asociada con variantes de escape. Dos muestras presentaron una deleción de 105 nucleótidos en la región preS1-preS2. Conclusiones. Se corroboró la circulación en Colombia de los genotipos y subgenotipos F3 y A2, así como la presencia de mutaciones de resistencia y escape. El presente estudio constituye un aporte a la epidemiologia molecular del HBV en Colombia. |
| publishDate |
2018 |
| dc.date.issued.none.fl_str_mv |
2018-08-01 |
| dc.date.accessioned.none.fl_str_mv |
2021-01-21T00:45:44Z |
| dc.date.available.none.fl_str_mv |
2021-01-21T00:45:44Z |
| dc.type.none.fl_str_mv |
Artículo |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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2590-7379 |
| dc.identifier.uri.spa.fl_str_mv |
10.7705/biomedica.v38i3.3871 |
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https://hdl.handle.net/20.500.12494/32708 |
| dc.identifier.bibliographicCitation.spa.fl_str_mv |
Peláez-Carvajal, D., Forero, N. J., Escalante-Mora, M., Laiton-Donato, K., & Usme-Ciro, J. A. (2018). Variabilidad genética en regiones codificantes del antígeno de superficie y el dominio de la transcriptasa inversa de la polimerasa del virus de la hepatitis B, Colombia, 2002-2014. Biomédica, 38, 37–50. |
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2590-7379 10.7705/biomedica.v38i3.3871 Peláez-Carvajal, D., Forero, N. J., Escalante-Mora, M., Laiton-Donato, K., & Usme-Ciro, J. A. (2018). Variabilidad genética en regiones codificantes del antígeno de superficie y el dominio de la transcriptasa inversa de la polimerasa del virus de la hepatitis B, Colombia, 2002-2014. Biomédica, 38, 37–50. |
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https://hdl.handle.net/20.500.12494/32708 |
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https://revistabiomedica.org/index.php/biomedica/article/view/3871 |
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Biomedica |
| dc.relation.references.spa.fl_str_mv |
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Specific amino acid substitutions in the S protein prevent its excretion in vitro and may contribute to occult hepatitis B virus infection. J Virol. 2013;87:7882-92. https://doi.org/10.1128/JVI.00710-13 Coleman PF. Surveillance for hepatitis B surface antigen mutants. J Med Virol. 2006;78(Suppl.1):S56-8. https://doi. org/10.1002/jmv.20609/abstract Stuyver L, De Gendt S, van Geyt C, Zoulim F, Fried M, Schinazi RF, et al. A new genotype of hepatitis B virus: Complete genome and phylogenetic relatedness. J Gen Virol. 2000;81:67-74. https://doi.org/10.1099/0022-1317-81-1-67 Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013;30:2725-9. https://doi.org/10.1093/ molbev/mst197 Darriba D, Taboada GL, Doallo R, Posada D. jModelTest 2: More models, new heuristics and parallel computing. 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Gastroenterology. 2002;123:1848-56. https://doi. org/10.1053/gast.2002.37041 Norder H, Courouce AM, Magnius LO. Complete genomes, phylogenetic relatedness, and structural proteins of six strains of the hepatitis B virus, four of which represent two new genotypes. Virology. 1994;198:489-503. https://doi. org/10.1006/viro.1994.1060 Matsuura K, Tanaka Y, Hige S, Yamada G, Murawaki Y, Komatsu M, et al. Distribution of hepatitis B virus genotypes among patients with chronic infection in Japan shifting toward an increase of genotype A. J Clin Microbiol. 2009;47:1476-83. https://doi.org/10.1128/JCM.02081-08 Orito E, Mizokami M, Ina Y, Moriyama EN, Kameshima N, Yamamoto M, et al. Host-independent evolution and a genetic classification of the hepadnavirus family based on nucleotide sequences. Proc Natl Acad Sci USA. 1989;86: 7059-62 Girones R, Miller RH. Mutation rate of the hepadnavirus genome. Virology. 1989;170:595-7. Waters JA, Kennedy M, Voet P, Hauser P, Petre J, Carman W, et al. Loss of the common “A” determinant of hepatitis B surface antigen by a vaccine-induced escape mutant. J Clin Invest. 1992;90:2543-7. https://doi.org/10.1172/JCI116148 Carman WF, Korula J, Wallace L, MacPhee R, Mimms L, Decker R. Fulminant reactivation of hepatitis B due to envelope protein mutant that escaped detection by monoclonal HBsAg ELISA. Lancet. 1995;345:1406-7. Avellón A, Echevarría JM. Frequency of hepatitis B virus ‘a’ determinant variants in unselected Spanish chronic carriers. J Med Virol. 2006;78:24-36. https://doi.org/10.1002/ jmv.20516/abstract Kfoury-Baz EM, Zheng J, Mazuruk K, van Le A, Peterson DL. Characterization of a novel hepatitis B virus mutant: Demonstration of mutation-induced hepatitis B virus surface antigen group specific “a” determinant conformation change and its application in diagnostic assays. Transfus Med. 2001;11:355-62. https://doi.org/10.1046/j.1365-3148. 2001.00323.x Lee KM, Kim YS, Ko YY, Yoo BM, Lee KJ, Kim JH, et al. Emergence of vaccine-induced escape mutant of hepatitis B virus with multiple surface gene mutations in a Korean child. J Korean Med Sci. 2001;16:359-62. https://doi. org/10.3346/jkms.2001.16.3.359 Chen BF, Liu CJ, Jow GM, Chen PJ, Kao JH, Chen DS. High prevalence and mapping of pre-S deletion in hepatitis B virus carriers with progressive liver diseases. Gastroenterology. 2006;130:1153-68. https://doi.org/10. 1053/j.gastro.2006.01.011 Pollicino T, Amaddeo G, Restuccia A, Raffa G, Alibrandi A, Cutroneo G, et al. Impact of hepatitis B virus (HBV) preS/S genomic variability on HBV surface antigen and HBV DNA serum levels. Hepatology. 2012;56:434-43. https://doi. org/10.1002/hep.25592 Zhang D, Dong P, Zhang K, Deng L, Bach C, Chen W, et al. Whole genome HBV deletion profiles and the accumulation of preS deletion mutant during antiviral treatment. BMC Microbiol. 2012;12:307. https://doi.org/10.1186/1471-2180- 12-307 Santantonio T, Jung MC, Schneider R, Fernholz D, Milella M, Monno L, et al. Hepatitis B virus genomes that cannot synthesize pre-S2 proteins occur frequently and as dominant virus populations in chronic carriers in Italy. Virology. 1992;188:948-52. https://doi.org/10.1016/0042- 6822(92)90559-8 |
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Peláez Carvajal, DioselinaForero, Nidia J.Escalante Mora, MarthaLaiton Donato, KatherineUsme Ciro, José Aldemar382021-01-21T00:45:44Z2021-01-21T00:45:44Z2018-08-012590-737910.7705/biomedica.v38i3.3871https://hdl.handle.net/20.500.12494/32708Peláez-Carvajal, D., Forero, N. J., Escalante-Mora, M., Laiton-Donato, K., & Usme-Ciro, J. A. (2018). Variabilidad genética en regiones codificantes del antígeno de superficie y el dominio de la transcriptasa inversa de la polimerasa del virus de la hepatitis B, Colombia, 2002-2014. Biomédica, 38, 37–50.Introducción. Se estima que 240 millones de personas en el mundo tienen infección crónica con el virus de la hepatitis B (HBV). En Colombia, la endemia es variable y circulan diferentes genotipos virales. Las mutaciones a lo largo del genoma se han asociado con resistencia antiviral, el escape ante la reacción de anticuerpos neutralizadores tras la vacunación o a la infección natural, la infección oculta y la progresión a carcinoma hepatocelular. Objetivo. Identificar los genotipos y las mutaciones presentes en la región codificante del antígeno de superficie (S) y del dominio de la transcriptasa inversa (reverse transcriptase, RT) de la polimerasa del HBV en muestras de suero remitidas al Instituto Nacional de Salud de Colombia para el diagnóstico de hepatitis B, entre el 2002 y el 2014. Materiales y métodos. En 495 muestras de suero positivas para el antígeno de superficie de la hepatitis B (HBsAg) se buscó el ADN viral, se amplificó y secuenció un fragmento de 1.591 nucleótidos y, posteriormente, se hizo el análisis filogenético correspondiente. Resultados. En 66 de las muestras se logró detectar el genoma viral y 28 de ellas se secuenciaron exitosamente. El análisis filogenético permitió identificar los genotipos y subgenotipos F3 y A2. Una muestra presentó simultáneamente las sustituciones de resistencia L180M y M204V, otra presentó la sustitución I169L y en una se identificó la mutación P120Q, previamente asociada con variantes de escape. Dos muestras presentaron una deleción de 105 nucleótidos en la región preS1-preS2. Conclusiones. Se corroboró la circulación en Colombia de los genotipos y subgenotipos F3 y A2, así como la presencia de mutaciones de resistencia y escape. El presente estudio constituye un aporte a la epidemiologia molecular del HBV en Colombia.https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000318507https://orcid.org/0000-0002-8093-0544https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000008981jose.usmec@campusucc.edu.cohttps://scholar.google.com.co/citations?user=cU2KyT4AAAAJ&hl=en14Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa MartaDr. Luis Alberto GomezMedicinaSanta Martahttps://revistabiomedica.org/index.php/biomedica/article/view/3871BiomedicaDienstag JL. Hepatitis B virus infection. N Engl J Med. 2008; 359:1486-500. https://doi.org/10.1056/NEJMra0801644Paganelli M, Stephenne X, Sokal EM. Chronic hepatitis B in children and adolescents. J Hepatol. 2012;57:885-96. https://doi.org/10.1016/j.jhep.2012.03.036World Health Organization. Hepatitis B. Fact sheet. 2016. Fecha de consulta: 1° de julio de 2017. 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Virology. 1992;188:948-52. https://doi.org/10.1016/0042- 6822(92)90559-8Virus de la hepatitis BGenotipoADN Polimerasa dirigida por ARNMutaciónHepatitis B virusGenotypeMutationRNA Dependent DNA PolymeraseVariabilidad genética en regiones codificantes del antígeno de superficie y dominio transcriptasa inversa de la polimerasa del virus de la hepatitis B, Colombia, 2002-2014Artículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribucióninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationORIGINAL19. Variabilidad genética HBV.pdf19. 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