Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections

Background: The transmission of Dengue virus (DENV) and Chikungunya virus (CHIKV) has increased worldwide, due in part to the lack of a specific antiviral treatment. For this reason, the search for compounds with antiviral potential, either as licensed drugs or in natural products, is a research pri...

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Autores:
Martínez Gutiérrez, Marlén
Tipo de recurso:
Article of journal
Fecha de publicación:
2017
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/4951
Acceso en línea:
https://hdl.handle.net/20.500.12494/4951
Palabra clave:
Dengue
Aniviral
Rights
openAccess
License
Licencia CC
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dc.title.spa.fl_str_mv Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections
title Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections
spellingShingle Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections
Dengue
Aniviral
title_short Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections
title_full Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections
title_fullStr Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections
title_full_unstemmed Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections
title_sort Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections
dc.creator.fl_str_mv Martínez Gutiérrez, Marlén
dc.contributor.author.none.fl_str_mv Martínez Gutiérrez, Marlén
dc.subject.spa.fl_str_mv Dengue
Aniviral
topic Dengue
Aniviral
description Background: The transmission of Dengue virus (DENV) and Chikungunya virus (CHIKV) has increased worldwide, due in part to the lack of a specific antiviral treatment. For this reason, the search for compounds with antiviral potential, either as licensed drugs or in natural products, is a research priority. The objective of this study was to identify some of the compounds that are present in Mammea americana (M. americana) and Tabernaemontana cymosa (T. cymosa) plants and, subsequently, to evaluate their cytotoxicity in VERO cells and their potential antiviral effects on DENV and CHIKV infections in those same cells. Methods: Dry ethanolic extracts of M. americana and T. cymosa seeds were subjected to open column chromatographic fractionation, leading to the identification of four compounds: two coumarins, derived from M. americana; and lupeol acetate and voacangine derived from T. cymosa.. The cytotoxicity of each compound was subsequently assessed by the MTT method (at concentrations from 400 to 6.25 μg/mL). Pre- and post-treatment antiviral assays were performed at non-toxic concentrations; the resulting DENV inhibition was evaluated by Real-Time PCR, and the CHIKV inhibition was tested by the plating method. The results were analyzed by means of statistical analysis. Results: The compounds showed low toxicity at concentrations ≤ 200 μg/mL. The compounds coumarin A and coumarin B, which are derived from the M. americana plant, significantly inhibited infection with both viruses during the implementation of the two experimental strategies employed here (post-treatment with inhibition percentages greater than 50%, p < 0.01; and pre-treatment with percentages of inhibition greater than 40%, p < 0.01). However, the lupeol acetate and voacangine compounds, which were derived from the T. cymosa plant, only significantly inhibited the DENV infection during the post-treatment strategy (at inhibition percentages greater than 70%, p < 0.01). Conclusion: In vitro, the coumarins are capable of inhibiting infection by DENV and CHIKV (with inhibition percentages above 50% in different experimental strategies), which could indicate that these two compounds are potential antivirals for treating Dengue and Chikungunya fever. Additionally, lupeol acetate and voacangine efficiently inhibit infection with DENV, also turning them into promising antivirals for Dengue fever.
publishDate 2017
dc.date.issued.none.fl_str_mv 2017-01-18
dc.date.accessioned.none.fl_str_mv 2018-07-23T17:03:40Z
dc.date.available.none.fl_str_mv 2018-07-23T17:03:40Z
dc.type.none.fl_str_mv Artículo
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dc.identifier.bibliographicCitation.spa.fl_str_mv Gómez-Calderón et al. BMC Complementary and Alternative Medicine (2017) 17:57 DOI 10.1186/s12906-017-1562-1
url https://hdl.handle.net/20.500.12494/4951
identifier_str_mv Gómez-Calderón et al. BMC Complementary and Alternative Medicine (2017) 17:57 DOI 10.1186/s12906-017-1562-1
dc.relation.isversionof.spa.fl_str_mv https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5241984/pdf/12906_2017_Article_1562.pdf
dc.rights.cc.none.fl_str_mv Licencia CC
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eu_rights_str_mv openAccess
dc.publisher.spa.fl_str_mv Grupo de Investigación en Ciencias Animales-GRICA, Universidad Cooperativa de Colombia
dc.publisher.program.spa.fl_str_mv Medicina veterinaria y zootecnia
dc.publisher.place.spa.fl_str_mv Bucaramanga
institution Universidad Cooperativa de Colombia
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spelling Martínez Gutiérrez, Marlén2018-07-23T17:03:40Z2018-07-23T17:03:40Z2017-01-18https://hdl.handle.net/20.500.12494/4951Gómez-Calderón et al. BMC Complementary and Alternative Medicine (2017) 17:57 DOI 10.1186/s12906-017-1562-1Background: The transmission of Dengue virus (DENV) and Chikungunya virus (CHIKV) has increased worldwide, due in part to the lack of a specific antiviral treatment. For this reason, the search for compounds with antiviral potential, either as licensed drugs or in natural products, is a research priority. The objective of this study was to identify some of the compounds that are present in Mammea americana (M. americana) and Tabernaemontana cymosa (T. cymosa) plants and, subsequently, to evaluate their cytotoxicity in VERO cells and their potential antiviral effects on DENV and CHIKV infections in those same cells. Methods: Dry ethanolic extracts of M. americana and T. cymosa seeds were subjected to open column chromatographic fractionation, leading to the identification of four compounds: two coumarins, derived from M. americana; and lupeol acetate and voacangine derived from T. cymosa.. The cytotoxicity of each compound was subsequently assessed by the MTT method (at concentrations from 400 to 6.25 μg/mL). Pre- and post-treatment antiviral assays were performed at non-toxic concentrations; the resulting DENV inhibition was evaluated by Real-Time PCR, and the CHIKV inhibition was tested by the plating method. The results were analyzed by means of statistical analysis. Results: The compounds showed low toxicity at concentrations ≤ 200 μg/mL. The compounds coumarin A and coumarin B, which are derived from the M. americana plant, significantly inhibited infection with both viruses during the implementation of the two experimental strategies employed here (post-treatment with inhibition percentages greater than 50%, p < 0.01; and pre-treatment with percentages of inhibition greater than 40%, p < 0.01). However, the lupeol acetate and voacangine compounds, which were derived from the T. cymosa plant, only significantly inhibited the DENV infection during the post-treatment strategy (at inhibition percentages greater than 70%, p < 0.01). Conclusion: In vitro, the coumarins are capable of inhibiting infection by DENV and CHIKV (with inhibition percentages above 50% in different experimental strategies), which could indicate that these two compounds are potential antivirals for treating Dengue and Chikungunya fever. Additionally, lupeol acetate and voacangine efficiently inhibit infection with DENV, also turning them into promising antivirals for Dengue fever.marlen.martinezg@campusucc.edu.coGrupo de Investigación en Ciencias Animales-GRICA, Universidad Cooperativa de ColombiaMedicina veterinaria y zootecniaBucaramangahttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5241984/pdf/12906_2017_Article_1562.pdfDengueAniviralAntiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infectionsArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionLicencia CCinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2https://orcid.org/0000-0002-9429-0058PublicationORIGINALGómez-Calderón et al. 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