Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections
Background: The transmission of Dengue virus (DENV) and Chikungunya virus (CHIKV) has increased worldwide, due in part to the lack of a specific antiviral treatment. For this reason, the search for compounds with antiviral potential, either as licensed drugs or in natural products, is a research pri...
- Autores:
-
Martínez Gutiérrez, Marlén
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2017
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/4951
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/4951
- Palabra clave:
- Dengue
Aniviral
- Rights
- openAccess
- License
- Licencia CC
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dc.title.spa.fl_str_mv |
Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections |
title |
Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections |
spellingShingle |
Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections Dengue Aniviral |
title_short |
Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections |
title_full |
Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections |
title_fullStr |
Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections |
title_full_unstemmed |
Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections |
title_sort |
Antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections |
dc.creator.fl_str_mv |
Martínez Gutiérrez, Marlén |
dc.contributor.author.none.fl_str_mv |
Martínez Gutiérrez, Marlén |
dc.subject.spa.fl_str_mv |
Dengue Aniviral |
topic |
Dengue Aniviral |
description |
Background: The transmission of Dengue virus (DENV) and Chikungunya virus (CHIKV) has increased worldwide, due in part to the lack of a specific antiviral treatment. For this reason, the search for compounds with antiviral potential, either as licensed drugs or in natural products, is a research priority. The objective of this study was to identify some of the compounds that are present in Mammea americana (M. americana) and Tabernaemontana cymosa (T. cymosa) plants and, subsequently, to evaluate their cytotoxicity in VERO cells and their potential antiviral effects on DENV and CHIKV infections in those same cells. Methods: Dry ethanolic extracts of M. americana and T. cymosa seeds were subjected to open column chromatographic fractionation, leading to the identification of four compounds: two coumarins, derived from M. americana; and lupeol acetate and voacangine derived from T. cymosa.. The cytotoxicity of each compound was subsequently assessed by the MTT method (at concentrations from 400 to 6.25 μg/mL). Pre- and post-treatment antiviral assays were performed at non-toxic concentrations; the resulting DENV inhibition was evaluated by Real-Time PCR, and the CHIKV inhibition was tested by the plating method. The results were analyzed by means of statistical analysis. Results: The compounds showed low toxicity at concentrations ≤ 200 μg/mL. The compounds coumarin A and coumarin B, which are derived from the M. americana plant, significantly inhibited infection with both viruses during the implementation of the two experimental strategies employed here (post-treatment with inhibition percentages greater than 50%, p < 0.01; and pre-treatment with percentages of inhibition greater than 40%, p < 0.01). However, the lupeol acetate and voacangine compounds, which were derived from the T. cymosa plant, only significantly inhibited the DENV infection during the post-treatment strategy (at inhibition percentages greater than 70%, p < 0.01). Conclusion: In vitro, the coumarins are capable of inhibiting infection by DENV and CHIKV (with inhibition percentages above 50% in different experimental strategies), which could indicate that these two compounds are potential antivirals for treating Dengue and Chikungunya fever. Additionally, lupeol acetate and voacangine efficiently inhibit infection with DENV, also turning them into promising antivirals for Dengue fever. |
publishDate |
2017 |
dc.date.issued.none.fl_str_mv |
2017-01-18 |
dc.date.accessioned.none.fl_str_mv |
2018-07-23T17:03:40Z |
dc.date.available.none.fl_str_mv |
2018-07-23T17:03:40Z |
dc.type.none.fl_str_mv |
Artículo |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.version.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12494/4951 |
dc.identifier.bibliographicCitation.spa.fl_str_mv |
Gómez-Calderón et al. BMC Complementary and Alternative Medicine (2017) 17:57 DOI 10.1186/s12906-017-1562-1 |
url |
https://hdl.handle.net/20.500.12494/4951 |
identifier_str_mv |
Gómez-Calderón et al. BMC Complementary and Alternative Medicine (2017) 17:57 DOI 10.1186/s12906-017-1562-1 |
dc.relation.isversionof.spa.fl_str_mv |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5241984/pdf/12906_2017_Article_1562.pdf |
dc.rights.cc.none.fl_str_mv |
Licencia CC |
dc.rights.accessrights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.coar.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
rights_invalid_str_mv |
Licencia CC http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.publisher.spa.fl_str_mv |
Grupo de Investigación en Ciencias Animales-GRICA, Universidad Cooperativa de Colombia |
dc.publisher.program.spa.fl_str_mv |
Medicina veterinaria y zootecnia |
dc.publisher.place.spa.fl_str_mv |
Bucaramanga |
institution |
Universidad Cooperativa de Colombia |
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spelling |
Martínez Gutiérrez, Marlén2018-07-23T17:03:40Z2018-07-23T17:03:40Z2017-01-18https://hdl.handle.net/20.500.12494/4951Gómez-Calderón et al. BMC Complementary and Alternative Medicine (2017) 17:57 DOI 10.1186/s12906-017-1562-1Background: The transmission of Dengue virus (DENV) and Chikungunya virus (CHIKV) has increased worldwide, due in part to the lack of a specific antiviral treatment. For this reason, the search for compounds with antiviral potential, either as licensed drugs or in natural products, is a research priority. The objective of this study was to identify some of the compounds that are present in Mammea americana (M. americana) and Tabernaemontana cymosa (T. cymosa) plants and, subsequently, to evaluate their cytotoxicity in VERO cells and their potential antiviral effects on DENV and CHIKV infections in those same cells. Methods: Dry ethanolic extracts of M. americana and T. cymosa seeds were subjected to open column chromatographic fractionation, leading to the identification of four compounds: two coumarins, derived from M. americana; and lupeol acetate and voacangine derived from T. cymosa.. The cytotoxicity of each compound was subsequently assessed by the MTT method (at concentrations from 400 to 6.25 μg/mL). Pre- and post-treatment antiviral assays were performed at non-toxic concentrations; the resulting DENV inhibition was evaluated by Real-Time PCR, and the CHIKV inhibition was tested by the plating method. The results were analyzed by means of statistical analysis. Results: The compounds showed low toxicity at concentrations ≤ 200 μg/mL. The compounds coumarin A and coumarin B, which are derived from the M. americana plant, significantly inhibited infection with both viruses during the implementation of the two experimental strategies employed here (post-treatment with inhibition percentages greater than 50%, p < 0.01; and pre-treatment with percentages of inhibition greater than 40%, p < 0.01). However, the lupeol acetate and voacangine compounds, which were derived from the T. cymosa plant, only significantly inhibited the DENV infection during the post-treatment strategy (at inhibition percentages greater than 70%, p < 0.01). Conclusion: In vitro, the coumarins are capable of inhibiting infection by DENV and CHIKV (with inhibition percentages above 50% in different experimental strategies), which could indicate that these two compounds are potential antivirals for treating Dengue and Chikungunya fever. Additionally, lupeol acetate and voacangine efficiently inhibit infection with DENV, also turning them into promising antivirals for Dengue fever.marlen.martinezg@campusucc.edu.coGrupo de Investigación en Ciencias Animales-GRICA, Universidad Cooperativa de ColombiaMedicina veterinaria y zootecniaBucaramangahttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5241984/pdf/12906_2017_Article_1562.pdfDengueAniviralAntiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infectionsArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionLicencia CCinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2https://orcid.org/0000-0002-9429-0058PublicationORIGINALGómez-Calderón et al. BMC Complementary and Alternative Medicine (2017) 17.57.pdfGómez-Calderón et al. BMC Complementary and Alternative Medicine (2017) 17.57.pdfapplication/pdf894124https://repository.ucc.edu.co/bitstreams/661a4994-0b2b-468d-8d0b-f943e05afce9/download434153301c1fb939b729170a742521e3MD51Licencia de uso.pdfLicencia de uso.pdfapplication/pdf469522https://repository.ucc.edu.co/bitstreams/43d7795a-1339-449a-99a0-a2e5e6a0168f/download434a1aa0cdcc04c7bb8bb2cc99c87145MD55LICENSElicense.txtlicense.txttext/plain; charset=utf-84514https://repository.ucc.edu.co/bitstreams/b2dd659d-1508-47d3-a46d-7e5b31ba88d8/downloadf3f5e184cacb7496a3e3345ca148d0f7MD52THUMBNAILGómez-Calderón et al. BMC Complementary and Alternative Medicine (2017) 17.57.pdf.jpgGómez-Calderón et al. 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