Effects of density of anisotropic microstamped silica thin films on guided bone tissue regeneration - In vitro study

The growing demand for better implant aesthetics has led to increased research on the development of all-ceramic dental implants. The use of microtextured coatings with enhanced properties has been presented as a viable way to improve tissue integrability of all-ceramic implants. The aim of this study was to evaluate the effects of different densities of anisotropic microtextured silica thin films, which served as a model coating, on the behavior of human osteoblast-like cells. The differential responses of human osteoblast-like cells to anisotropic silica microtextures with varying densities, produced via a combination of sol-gel and soft lithography processing, were evaluated in terms of alignment, elongation (using fluorescence microscopy), overall cellular activity, and the expression/activity levels of alkaline phosphatase (ALP). Statistical analysis was conducted using one-way ANOVA/Tukey HSD post hoc test. The thin films were thoroughly characterized via scanning electron microscopy/energy dispersive spectroscopy, Fourier transform infrared, and contact angle measurements. Thin film characterization revealed increased nanoscale roughness and reduced wettability on the micropatterned surfaces. Cell culture experiments indicated that the microtextures induced cell alignment, elongation, and guided colonization on the surface. Cells cultured on denser micropatterns exhibited increased metabolic activity (t = 14-21 days). The early expression/activity levels of ALP released into the medium were found to be significantly higher only on the least dense micropattern. These results suggest the possibility that microstructured silica thin films could be used to guide and enhance peri-implant cell/tissue responses, potentially improving tissue integration for metallic and all-ceramic dental implants. © 2013 Wiley Periodicals, Inc.

• Autores: Pelaez Vargas, Alejandro; Gallego-Perez D.; Carvalho A.; Fernandes M.H.; Hansford D.J.; Monteiro F.J.
• Tipo de recurso: Article of journal
• Fecha de publicación: 2013
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/41374
• Acceso en línea: https://doi.org/10.1002/jdd.12183; https://hdl.handle.net/20.500.12494/41374
• Palabra clave: Bone tissue regeneration; Cell-material interaction; Differential response; Fourier transform infra reds; Human osteoblast-like cells; Micropatterned surface; Nano-scale roughness; Thin-film characterization; ALkaline phosphatase; Enhanced properties; Alignment; Anisotropy; Bone; Cell culture; Cell proliferation; Ceramic materials; Coatings; Contact angle; Dental prostheses; Fluorescence microscopy; Phosphatases; Silica; Sol-gels; Tissue; Energy dispersive spectroscopy; Thin films; alkaline phosphatase; anisotropic microstamped silica thin film; dental material; unclassified drug; nanofilm; silicon dioxide; article; biocompatibility; bone regeneration; cell activity; cell culture; cell metabolism; colony formation; contact angle; controlled study; density; energy dispersive spectroscopy; enzyme activity; fluorescence microscopy; guided bone tissue regeneration; human; human cell; in vitro study; infrared spectroscopy; osteoblast; physical parameters; protein expression; roughness; scan
• Rights: closedAccess
• License: http://purl.org/coar/access_right/c_14cb