Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga

Artículo científico

Autores:
Estrada Gómez, Sebastián
Vargas Muñoz, Leidy Johana
Saldarriaga Córdoba, Mónica María
Cifuentes, Yeimy
Perafán, Carlos
Tipo de recurso:
Article of journal
Fecha de publicación:
2017
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/3367
Acceso en línea:
https://hdl.handle.net/20.500.12494/3367
Palabra clave:
Pamphobeteus verdolaga
Theraphosidae
Transcriptomic
Proteomic
Rights
openAccess
License
Licencia CC
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oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/3367
network_acronym_str COOPER2
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dc.title.spa.fl_str_mv Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
title Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
spellingShingle Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
Pamphobeteus verdolaga
Theraphosidae
Transcriptomic
Proteomic
title_short Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
title_full Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
title_fullStr Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
title_full_unstemmed Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
title_sort Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga
dc.creator.fl_str_mv Estrada Gómez, Sebastián
Vargas Muñoz, Leidy Johana
Saldarriaga Córdoba, Mónica María
Cifuentes, Yeimy
Perafán, Carlos
dc.contributor.author.none.fl_str_mv Estrada Gómez, Sebastián
Vargas Muñoz, Leidy Johana
Saldarriaga Córdoba, Mónica María
Cifuentes, Yeimy
Perafán, Carlos
dc.subject.spa.fl_str_mv Pamphobeteus verdolaga
Theraphosidae
Transcriptomic
Proteomic
topic Pamphobeteus verdolaga
Theraphosidae
Transcriptomic
Proteomic
description Artículo científico
publishDate 2017
dc.date.issued.none.fl_str_mv 2017-02-13
dc.date.accessioned.none.fl_str_mv 2018-04-10T19:48:39Z
dc.date.available.none.fl_str_mv 2018-04-10T19:48:39Z
dc.type.none.fl_str_mv Artículo
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dc.identifier.bibliographicCitation.spa.fl_str_mv Estrada Gomez, S., Vargas Muñoz, L. J., Saldarriaga Cordoba, M., Cifuentes, Y., y Perafan, C., (2017). Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga. Toxicon 129 (2017) 81-88. http://dx.doi.org/10.1016/j.toxicon.2017.02.004
url https://hdl.handle.net/20.500.12494/3367
identifier_str_mv Estrada Gomez, S., Vargas Muñoz, L. J., Saldarriaga Cordoba, M., Cifuentes, Y., y Perafan, C., (2017). Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga. Toxicon 129 (2017) 81-88. http://dx.doi.org/10.1016/j.toxicon.2017.02.004
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rights_invalid_str_mv Licencia CC
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eu_rights_str_mv openAccess
dc.publisher.spa.fl_str_mv Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Medellín y Envigado
dc.publisher.program.spa.fl_str_mv Medicina
dc.publisher.place.spa.fl_str_mv Medellín
institution Universidad Cooperativa de Colombia
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spelling Estrada Gómez, SebastiánVargas Muñoz, Leidy JohanaSaldarriaga Córdoba, Mónica MaríaCifuentes, YeimyPerafán, Carlos2018-04-10T19:48:39Z2018-04-10T19:48:39Z2017-02-13https://hdl.handle.net/20.500.12494/3367Estrada Gomez, S., Vargas Muñoz, L. J., Saldarriaga Cordoba, M., Cifuentes, Y., y Perafan, C., (2017). Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga. Toxicon 129 (2017) 81-88. http://dx.doi.org/10.1016/j.toxicon.2017.02.004Artículo científicoTheraphosidae spider venoms are well known for possess a complex mixture of protein and non-protein compounds in their venom. The objective of this study was to report and identify different proteins translated from the venom gland DNA information of the recently described Theraphosidae spider Pamphobeteus verdolaga. Using a venom gland transcriptomic analysis, we reported a set of the first complete sequences of seven different proteins of the recenlty described Theraphosidae spider P. verdolaga. Protein analysis indicates the presence of different proteins on the venom composition of this new spider, some of them uncommon in the Theraphosidae family. MS/MS analysis of P. verdolaga showed different fragments matching sphingomyelinases (sicaritoxin), barytoxins, hexatoxins, latroinsectotoxins, and linear (zadotoxins) peptides. Only four of the MS/MS fragments showed 100% sequence similarity with one of the transcribed proteins. Transcriptomic analysis showed the presence of different groups of proteins like phospholipases, hyaluronidases, inhibitory cysteine knots (ICK) peptides among others. The three database of protein domains used in this study (Pfam, SMART and CDD) showed congruency in the search of unique conserved protein domain for only four of the translated proteins. Those proteins matched with EF-hand proteins, cysteine rich secretory proteins, jingzhaotoxins, theraphotoxins and hexatoxins, from different Mygalomorphae spiders belonging to the families Theraphosidae, Barychelidae and Hexathelidae. None of the analyzed sequences showed a complete 100% similaritysebastian.estrada@udea.edu.coleidy. vargasmu@campusucc.edu.comonica.saldarriaga@uibero.clyelicigi@gmail.comcaperafanl@gmail.comUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Medellín y EnvigadoMedicinaMedellínhttps://www.sciencedirect.com/science/article/pii/S0041010117300508?via%3DihubPamphobeteus verdolagaTheraphosidaeTranscriptomicProteomicIdentifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolagaArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionLicencia CCinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationORIGINALIdentifying different transcribed proteins in the newly described.pdfIdentifying different transcribed proteins in the newly described.pdfapplication/pdf1813547https://repository.ucc.edu.co/bitstreams/68dff5ca-d148-4a86-b531-51900b8f3fa8/download6514a8e8b9f00cb2f38742cd84daec48MD51Formato Licencia de uso.pdfFormato Licencia de uso.pdfapplication/pdf215914https://repository.ucc.edu.co/bitstreams/507076c7-865f-4abb-9eb4-9a3b69fa0a13/downloadb9be3f3834de28f7c374f809ae0bd756MD53TEXTIdentifying different transcribed proteins in the newly described.pdf.txtIdentifying different transcribed proteins in the newly described.pdf.txtExtracted texttext/plain35647https://repository.ucc.edu.co/bitstreams/6c54f4d1-20d4-4d25-9b49-319d290a4a44/downloadfed84feaaf33b44270a3d2c53237bcafMD54Formato Licencia de uso.pdf.txtFormato Licencia de uso.pdf.txtExtracted texttext/plain4392https://repository.ucc.edu.co/bitstreams/54185792-cec2-433a-994d-8e9be6167c5f/download2c43f0d1167c388d6994b2d4b0bd22efMD56LICENSElicense.txtlicense.txttext/plain; charset=utf-84514https://repository.ucc.edu.co/bitstreams/7ceb3d5e-4e8e-45cb-bc7b-e25bc4ef39b1/downloadf3f5e184cacb7496a3e3345ca148d0f7MD52THUMBNAILIdentifying different transcribed proteins in the newly described.pdf.jpgIdentifying different transcribed proteins in the newly described.pdf.jpgIM Thumbnailimage/jpeg5986https://repository.ucc.edu.co/bitstreams/03890c5c-c782-4dff-b778-9b273f2b9b05/download8afcec1433caefe96ceceaffa64d1630MD55Formato Licencia de uso.pdf.jpgFormato Licencia de uso.pdf.jpgIM Thumbnailimage/jpeg5313https://repository.ucc.edu.co/bitstreams/38880efc-5d15-4e7e-b485-147625b41a95/downloadcfb79eba048755a48755b0af7d620784MD5720.500.12494/3367oai:repository.ucc.edu.co:20.500.12494/33672024-08-10 22:45:34.305open.accesshttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com

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