Molecular detection of leptosipira in synanthropic and wild rodents from Villavicencio municipality, Colombia
Introduction: Rodents are potential transmitters of Leptospira spp. In the municipality of Villavicencio, Colombia, leptospirosis is a disease that, although notifiable, is still underreported. In this region, rodent species that can host pathogenic leptospira remain unknown. Objective: To detect th...
- Autores:
-
Sánchez Lerma, Liliana
Rojas Gulloso, Andrés Camilo
Contreras Cogollo, Verónica
Pavas Escobar, Norma Cristina
Barajas Pardo, Diana
Mattar Velilla, Salim
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2022
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/46837
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/46837
- Palabra clave:
- Roedores
Reservorios
Leptospiras patogenicas
Villavicencio
Rodents
Reservoirs
Pathogenic Leptospira
Villavicencio
- Rights
- openAccess
- License
- Atribución
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Molecular detection of leptosipira in synanthropic and wild rodents from Villavicencio municipality, Colombia |
| title |
Molecular detection of leptosipira in synanthropic and wild rodents from Villavicencio municipality, Colombia |
| spellingShingle |
Molecular detection of leptosipira in synanthropic and wild rodents from Villavicencio municipality, Colombia Roedores Reservorios Leptospiras patogenicas Villavicencio Rodents Reservoirs Pathogenic Leptospira Villavicencio |
| title_short |
Molecular detection of leptosipira in synanthropic and wild rodents from Villavicencio municipality, Colombia |
| title_full |
Molecular detection of leptosipira in synanthropic and wild rodents from Villavicencio municipality, Colombia |
| title_fullStr |
Molecular detection of leptosipira in synanthropic and wild rodents from Villavicencio municipality, Colombia |
| title_full_unstemmed |
Molecular detection of leptosipira in synanthropic and wild rodents from Villavicencio municipality, Colombia |
| title_sort |
Molecular detection of leptosipira in synanthropic and wild rodents from Villavicencio municipality, Colombia |
| dc.creator.fl_str_mv |
Sánchez Lerma, Liliana Rojas Gulloso, Andrés Camilo Contreras Cogollo, Verónica Pavas Escobar, Norma Cristina Barajas Pardo, Diana Mattar Velilla, Salim |
| dc.contributor.author.none.fl_str_mv |
Sánchez Lerma, Liliana Rojas Gulloso, Andrés Camilo Contreras Cogollo, Verónica Pavas Escobar, Norma Cristina Barajas Pardo, Diana Mattar Velilla, Salim |
| dc.subject.spa.fl_str_mv |
Roedores Reservorios Leptospiras patogenicas Villavicencio |
| topic |
Roedores Reservorios Leptospiras patogenicas Villavicencio Rodents Reservoirs Pathogenic Leptospira Villavicencio |
| dc.subject.other.spa.fl_str_mv |
Rodents Reservoirs Pathogenic Leptospira Villavicencio |
| description |
Introduction: Rodents are potential transmitters of Leptospira spp. In the municipality of Villavicencio, Colombia, leptospirosis is a disease that, although notifiable, is still underreported. In this region, rodent species that can host pathogenic leptospira remain unknown. Objective: To detect the presence of Leptospira spp. through molecular analysis in rodents (Rodentia) from peri-urban and rural areas belonging to the municipality of Villavicencio in Colombia. Methods: Peri-urban and rural areas of the townships belonging to Villavicencio municipality were selected for sampling. These areas presented similar ecological conditions: they were near water bodies and peridomiciliary areas, and some of them included fields of agricultural crops. Rodents´ kidneys were removed and frozen in liquid nitrogen. DNA was extracted using a commercial kit and subsequently amplified through conventional polymerase chain reaction. Results: The rodent species collected were: Rattus rattus, Mus musculus, Zygodontomys brevicauda, Oligoryzomys sp, Hylaeamys (formerly Oryzomys) and Proechimys cf. oconnelli. Leptospira DNA was amplified in six rodents and the purified amplicons were sent to Macrogen Inc. (Seoul, Korea) for sequencing. The alignment analysis of the sequenced products demonstrated 98.64% of coverage and identity with Leptospira interrogans. Conclusions: This is the first study carried out on wild and synanthropic rodents in the municipality of Villavicencio. The incidence of leptospirosis raises the alarm due to the important role of these small mammals in the transmission of this zoonosis, which is considered the second cause, after dengue, of undifferentiated febrile illness in Villavicencio. |
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2022 |
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2022-10-22T16:22:49Z |
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2022-10-22T16:22:49Z |
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2022-09-03 |
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Sánchez-Lerma L, Rojas-Gulloso A, Contreras-Cogollo V, Pavas-Escobar N, Barajas-Pardo D, Mattar-Velilla S. (2022) Molecular detection of pathogenic Leptospira in synanthropic and wild rodents captured in areas belonging to municipality of Villavicencio, Colombia. Revista Cubana de Medicina Tropical [Internet]. 2022 [citado 6 Oct 2022]; 74 (2) Disponible en: http://www.revmedtropical.sld.cu/index.php/medtropical/article/view/830.https://repository.ucc.edu.co/handle/20.500.12494/46837 |
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1561-3054 Sánchez-Lerma L, Rojas-Gulloso A, Contreras-Cogollo V, Pavas-Escobar N, Barajas-Pardo D, Mattar-Velilla S. (2022) Molecular detection of pathogenic Leptospira in synanthropic and wild rodents captured in areas belonging to municipality of Villavicencio, Colombia. Revista Cubana de Medicina Tropical [Internet]. 2022 [citado 6 Oct 2022]; 74 (2) Disponible en: http://www.revmedtropical.sld.cu/index.php/medtropical/article/view/830.https://repository.ucc.edu.co/handle/20.500.12494/46837 |
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Revista Cubana de Medicina Tropical |
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Castellar A, Arrieta P, Caraballo L, Torres M, Ríos L. Detección de Leptospira spp. patógenas en tejido renal de ratón casero (Mus musculus) en Sincelejo, Colombia. Revista Biosalud. 2016;15(1):41-9. DOI: https://doi.org/10.17151/biosa.2016.15.1.5 Boey K, Shiokawa K, Sreekumari Rajeev S. Leptospira infection in rats: A literature review of global prevalence and distribution. PLOS NTD. 2019;13(8):e0007499. DOI: https://doi.org/10.1371/journal.pntd.0007499 Sánchez L. Dengue, leptospirosis, hantavirosis y rickettsiosis en pacientes con síndrome febril agudo no palúdico en el Meta, Colombia (2013-2014). Instituto de Medicina Tropical Pedro Kouri; 2017. 4. Syakbanah N, Fuad A. Human leptospirosis outbreak: A year after the ‘Cempaka’ tropical cyclone. JKL. 2021;13(4): 211-8. Pellizzaro M, Martins C, Yamakawa A, Da Cunha D, Morikawa V, Ferreira F, et al. Molecular detection of Leptospira spp. in rats as early spatial predictor for human disease in an endemic urban area. PLoS ONE. 2019;14(5):e0216830. DOI: https://doi.org/10.1371/journal.pone.0216830 Valbuena C, Pefaur J. Determinación de leptospirosis en roedores y marsupiales de la región sur del lago de Maracaibo, Estado Mérida, Venezuela. Revista Científica. 2015;25(3):193-9. 7. Pérez J, Agudelo P, Parra G, Ochoa J, Arboleda M. Incidencia y subregistro de casos de leptospirosis diagnosticados con tres métodos diferentes en Urabá, Colombia. Biomédica. 2019;39(supl 1):150-62. DOI: https://doi.org/10.7705/biomedica.v39i0.4577 Calderón A, Rodríguez V, Guzmán C. Anticuerpos de Leptospira interrogans (sensu lato) en trabajadores de granjas porcinas del medio Sinú, Córdoba (Colombia) ¿enfermedad profesional? Rev Colombiana Cienc Anim. 2016;8(1):56-64 Ortiz D, Navarrete J, Pinto C. Prevalencia de leptospirosis en humanos en el Distrito Capital. Revista de Investigaciones en Seguridad Social y Salud. 2009;11:51-8. Romero M, Sánchez J, Hayek L. Prevalencia de anticuerpos contra Leptospira en población urbana humana y canina del Departamento del Tolima. Rev. Salud Pública. 2010;12(2):268-75. 11. DANE. Censo nacional de poblaciones y vivienda 2018-Colombia (Villavicencio). Colombia; 2018. Disponible en: https://sitios.dane.gov.co/cnpv/#!/ Sánchez L, García C, Mattar S, Rodríguez I. Seroprevalencia de leptospirosis en pacientes con síndrome febril no palúdico. Rev Cubana Med Trop. 2018;70(1):45-54. Rojas A. Vigilancia de agentes infecciosos transmitidos por roedores (Rodentia) del municipio de Villavicencio y su impacto en la salud pública. Universidad de Córdoba; 2021. Noda A, Rodríguez I, Rodríguez Y, Govín A, Fernández C, Obregón A. High sensitive PCR method for detection of pathogenic Leptospira spp. in paraffin-embedded tissues. Rev Inst Med Trop São Paulo. 2014;56(5):411-5. Tique V, Mattar S, Miranda J, Oviedo M, Noda A, Montes E, et al. Clinical and Epidemiological Status of Leptospirosis in a Tropical Caribbean Area of Colombia. BioMed Res Int. 2018;2018:8. DOI: https://doi.org/10.1155/2018/6473851 Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018;35(6):1547-9. DOI: https://doi.org/10.1093/molbev/msy096 Torres M, Cruz B, Medina R, Reyes B, Moguel C, Medina R, et al. Detección molecular de leptospiras patógenas en roedores sinantrópicos y silvestres capturados en Yucatán, México. Biomédica. 2018;38:51-8. DOI: https://doi.org/10.7705/biomedica.v38i3.3938 Méndez C, Benavides L, Esquivel A, Aldama A, Torres J, Gavaldón D, et al. Pesquisa serológica de Leptospira en roedores silvestres, bovinos, equinos y caninos en el noreste de México. Rev Salud Anim. 2013;35(1):25-32. Giraldo G, Orrego A, Betancurth A. Rodents as Leptospira reservoirs at swine farms of the central coffee grower’s area of Colombia. Arch Med Vet. 2002;34(1):69-78. DOI: http://dx.doi.org/10.4067/S0301-732X2002000100007 Ospina C, Rincón M, Soler D, Hernández P. Papel de los roedores en la transmisión de Leptospira spp. en granjas porcinas. Rev Salud Pública. 2017;19 (4): 555-61. 21. Siuce J, Calle S, Pinto C, Pacheco G, Salvatierra G. Identificación de serogrupos patógenos de leptospira en canes domésticos. Rev Inv Vet Perú. 2015;26(4):664-75. DOI: http://dx.doi.org/10.15381/rivep.v26i4.11221 |
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Sánchez Lerma, LilianaRojas Gulloso, Andrés CamiloContreras Cogollo, VerónicaPavas Escobar, Norma CristinaBarajas Pardo, DianaMattar Velilla, Salim742022-10-22T16:22:49Z2022-10-22T16:22:49Z2022-09-031561-3054https://hdl.handle.net/20.500.12494/46837Sánchez-Lerma L, Rojas-Gulloso A, Contreras-Cogollo V, Pavas-Escobar N, Barajas-Pardo D, Mattar-Velilla S. (2022) Molecular detection of pathogenic Leptospira in synanthropic and wild rodents captured in areas belonging to municipality of Villavicencio, Colombia. Revista Cubana de Medicina Tropical [Internet]. 2022 [citado 6 Oct 2022]; 74 (2) Disponible en: http://www.revmedtropical.sld.cu/index.php/medtropical/article/view/830.https://repository.ucc.edu.co/handle/20.500.12494/46837Introduction: Rodents are potential transmitters of Leptospira spp. In the municipality of Villavicencio, Colombia, leptospirosis is a disease that, although notifiable, is still underreported. In this region, rodent species that can host pathogenic leptospira remain unknown. Objective: To detect the presence of Leptospira spp. through molecular analysis in rodents (Rodentia) from peri-urban and rural areas belonging to the municipality of Villavicencio in Colombia. Methods: Peri-urban and rural areas of the townships belonging to Villavicencio municipality were selected for sampling. These areas presented similar ecological conditions: they were near water bodies and peridomiciliary areas, and some of them included fields of agricultural crops. Rodents´ kidneys were removed and frozen in liquid nitrogen. DNA was extracted using a commercial kit and subsequently amplified through conventional polymerase chain reaction. Results: The rodent species collected were: Rattus rattus, Mus musculus, Zygodontomys brevicauda, Oligoryzomys sp, Hylaeamys (formerly Oryzomys) and Proechimys cf. oconnelli. Leptospira DNA was amplified in six rodents and the purified amplicons were sent to Macrogen Inc. (Seoul, Korea) for sequencing. The alignment analysis of the sequenced products demonstrated 98.64% of coverage and identity with Leptospira interrogans. Conclusions: This is the first study carried out on wild and synanthropic rodents in the municipality of Villavicencio. The incidence of leptospirosis raises the alarm due to the important role of these small mammals in the transmission of this zoonosis, which is considered the second cause, after dengue, of undifferentiated febrile illness in Villavicencio.https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001051342https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001344825https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001383531https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001388613https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001098756https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000149063https://orcid.org/0000-0001-7024-3874https://orcid.org/0000-0003-0445-5814https://orcid.org/0000-0001-5380-2347https://orcid.org/0000-0002-4892-8831https://orcid.org/0000-0002-7604-4734https://orcid.org/0000-0003-0526-4630https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000009671https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000008981https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000000189liliana.sanchez@campusucc.edu.coandres.rojasgu@campusucc.edu.coverocontreras.helios@gmail.comnorma.pavas@campusucc.edu.codianap.barajas@campusucc.edu.cosmattar@correo.unicordoba.edu.cohttps://scholar.google.es/citations?hl=es&user=oPj-U5AAAAAJhttps://scholar.google.es/citations?hl=es&user=gwBokScAAAAJhttps://scholar.google.es/citations?hl=es&user=kqHNr_8AAAAJhttps://scholar.google.es/citations?hl=es&user=3W2cLHUAAAAJhttps://scholar.google.es/citations?hl=es&user=l4wtVfUAAAAJhttps://scholar.google.es/citations?hl=es&user=SIMzjtcAAAAJe830Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa MartaMedicinaSanta Martahttp://www.revmedtropical.sld.cu/index.php/medtropical/article/view/830Revista Cubana de Medicina TropicalCastellar A, Arrieta P, Caraballo L, Torres M, Ríos L. Detección de Leptospira spp. patógenas en tejido renal de ratón casero (Mus musculus) en Sincelejo, Colombia. Revista Biosalud. 2016;15(1):41-9. DOI: https://doi.org/10.17151/biosa.2016.15.1.5Boey K, Shiokawa K, Sreekumari Rajeev S. Leptospira infection in rats: A literature review of global prevalence and distribution. PLOS NTD. 2019;13(8):e0007499. DOI: https://doi.org/10.1371/journal.pntd.0007499Sánchez L. Dengue, leptospirosis, hantavirosis y rickettsiosis en pacientes con síndrome febril agudo no palúdico en el Meta, Colombia (2013-2014). Instituto de Medicina Tropical Pedro Kouri; 2017. 4. Syakbanah N, Fuad A. Human leptospirosis outbreak: A year after the ‘Cempaka’ tropical cyclone. JKL. 2021;13(4): 211-8.Pellizzaro M, Martins C, Yamakawa A, Da Cunha D, Morikawa V, Ferreira F, et al. Molecular detection of Leptospira spp. in rats as early spatial predictor for human disease in an endemic urban area. PLoS ONE. 2019;14(5):e0216830. DOI: https://doi.org/10.1371/journal.pone.0216830Valbuena C, Pefaur J. Determinación de leptospirosis en roedores y marsupiales de la región sur del lago de Maracaibo, Estado Mérida, Venezuela. Revista Científica. 2015;25(3):193-9. 7. Pérez J, Agudelo P, Parra G, Ochoa J, Arboleda M. Incidencia y subregistro de casos de leptospirosis diagnosticados con tres métodos diferentes en Urabá, Colombia. Biomédica. 2019;39(supl 1):150-62. DOI: https://doi.org/10.7705/biomedica.v39i0.4577Calderón A, Rodríguez V, Guzmán C. Anticuerpos de Leptospira interrogans (sensu lato) en trabajadores de granjas porcinas del medio Sinú, Córdoba (Colombia) ¿enfermedad profesional? Rev Colombiana Cienc Anim. 2016;8(1):56-64Ortiz D, Navarrete J, Pinto C. Prevalencia de leptospirosis en humanos en el Distrito Capital. Revista de Investigaciones en Seguridad Social y Salud. 2009;11:51-8.Romero M, Sánchez J, Hayek L. Prevalencia de anticuerpos contra Leptospira en población urbana humana y canina del Departamento del Tolima. Rev. Salud Pública. 2010;12(2):268-75. 11. DANE. Censo nacional de poblaciones y vivienda 2018-Colombia (Villavicencio). Colombia; 2018. Disponible en: https://sitios.dane.gov.co/cnpv/#!/Sánchez L, García C, Mattar S, Rodríguez I. Seroprevalencia de leptospirosis en pacientes con síndrome febril no palúdico. Rev Cubana Med Trop. 2018;70(1):45-54.Rojas A. Vigilancia de agentes infecciosos transmitidos por roedores (Rodentia) del municipio de Villavicencio y su impacto en la salud pública. Universidad de Córdoba; 2021.Noda A, Rodríguez I, Rodríguez Y, Govín A, Fernández C, Obregón A. High sensitive PCR method for detection of pathogenic Leptospira spp. in paraffin-embedded tissues. Rev Inst Med Trop São Paulo. 2014;56(5):411-5.Tique V, Mattar S, Miranda J, Oviedo M, Noda A, Montes E, et al. Clinical and Epidemiological Status of Leptospirosis in a Tropical Caribbean Area of Colombia. BioMed Res Int. 2018;2018:8. DOI: https://doi.org/10.1155/2018/6473851Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018;35(6):1547-9. DOI: https://doi.org/10.1093/molbev/msy096Torres M, Cruz B, Medina R, Reyes B, Moguel C, Medina R, et al. Detección molecular de leptospiras patógenas en roedores sinantrópicos y silvestres capturados en Yucatán, México. Biomédica. 2018;38:51-8. DOI: https://doi.org/10.7705/biomedica.v38i3.3938Méndez C, Benavides L, Esquivel A, Aldama A, Torres J, Gavaldón D, et al. Pesquisa serológica de Leptospira en roedores silvestres, bovinos, equinos y caninos en el noreste de México. Rev Salud Anim. 2013;35(1):25-32.Giraldo G, Orrego A, Betancurth A. Rodents as Leptospira reservoirs at swine farms of the central coffee grower’s area of Colombia. Arch Med Vet. 2002;34(1):69-78. DOI: http://dx.doi.org/10.4067/S0301-732X2002000100007Ospina C, Rincón M, Soler D, Hernández P. Papel de los roedores en la transmisión de Leptospira spp. en granjas porcinas. Rev Salud Pública. 2017;19 (4): 555-61. 21. Siuce J, Calle S, Pinto C, Pacheco G, Salvatierra G. Identificación de serogrupos patógenos de leptospira en canes domésticos. Rev Inv Vet Perú. 2015;26(4):664-75. 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