Influence of evidence, time, source and interferents in the observation of biological fluids with forensic lights

Introduction: The laboratory receives different evidence for analysis, which maycontain fluids such as blood, semen, saliva or urine. A support tool in identifying nonvisible biological stains is observation with forensic lights. At present, there have been research advances in reference to...

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Autores:
Laverde-Angarita, Lilia Judith
Clavijo-Bolívar, Yolanda
Tipo de recurso:
Article of journal
Fecha de publicación:
2015
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
spa
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/44314
Acceso en línea:
https://revistas.ucc.edu.co/index.php/ml/article/view/1215
https://hdl.handle.net/20.500.12494/44314
Palabra clave:
forensic sciences
biological fluids
forensic lights
evidence
ciencias forenses
fluidos biológicos
luces forenses
soportes
ciências forenses
fluídos biológicos
luzes forenses
suportes
Rights
openAccess
License
Derechos de autor 2015 Revista Colombia Forense
id COOPER2_12a78c696b964ef96580c263f32c8271
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/44314
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
dc.title.eng.fl_str_mv Influence of evidence, time, source and interferents in the observation of biological fluids with forensic lights
dc.title.spa.fl_str_mv Influencia de los soportes, tiempo, origen e interferentes en la observación de fluidos biológicos con luces forenses
dc.title.por.fl_str_mv Influência dos suportes, tempo, origem e interferentes na observação de fluídos biológicos com luzes forenses
title Influence of evidence, time, source and interferents in the observation of biological fluids with forensic lights
spellingShingle Influence of evidence, time, source and interferents in the observation of biological fluids with forensic lights
forensic sciences
biological fluids
forensic lights
evidence
ciencias forenses
fluidos biológicos
luces forenses
soportes
ciências forenses
fluídos biológicos
luzes forenses
suportes
title_short Influence of evidence, time, source and interferents in the observation of biological fluids with forensic lights
title_full Influence of evidence, time, source and interferents in the observation of biological fluids with forensic lights
title_fullStr Influence of evidence, time, source and interferents in the observation of biological fluids with forensic lights
title_full_unstemmed Influence of evidence, time, source and interferents in the observation of biological fluids with forensic lights
title_sort Influence of evidence, time, source and interferents in the observation of biological fluids with forensic lights
dc.creator.fl_str_mv Laverde-Angarita, Lilia Judith
Clavijo-Bolívar, Yolanda
dc.contributor.author.none.fl_str_mv Laverde-Angarita, Lilia Judith
Clavijo-Bolívar, Yolanda
dc.subject.eng.fl_str_mv forensic sciences
biological fluids
forensic lights
evidence
topic forensic sciences
biological fluids
forensic lights
evidence
ciencias forenses
fluidos biológicos
luces forenses
soportes
ciências forenses
fluídos biológicos
luzes forenses
suportes
dc.subject.spa.fl_str_mv ciencias forenses
fluidos biológicos
luces forenses
soportes
dc.subject.por.fl_str_mv ciências forenses
fluídos biológicos
luzes forenses
suportes
description Introduction: The laboratory receives different evidence for analysis, which maycontain fluids such as blood, semen, saliva or urine. A support tool in identifying nonvisible biological stains is observation with forensic lights. At present, there have been research advances in reference to wavelength and combination of different filters for the observation of biological fluids. Methodology: For this research, the alternate lights equipment Polilight® Flare with blue light was used, along with a filter and orange glasses, for observing stains of biological fluids deposited on different types of textiles and other surfaces that are frequently received by the laboratory. Results: The main result of this study was to determine the reliability of this equipment in terms of reproducibility andrepeatability of results, as well as the influence of variables such as substrate, age of the stain, source of fluid, and possible interferents in the observation of biological fluid fluorescence. Conclusions: It was found that biological stains emitting the strongest fluorescence were semen followed by urine and saliva stains. Bloodstains did not emit fluorescence and were referred to as non-fluorescent. Permeability of substrates made fluorescence halo greater, especially on light colors. Moreover, no statistically significant differences were observed in the fluorescence emission of azoospermic semen with respect to normspermic semen.
publishDate 2015
dc.date.accessioned.none.fl_str_mv 2022-03-02T16:45:17Z
dc.date.available.none.fl_str_mv 2022-03-02T16:45:17Z
dc.date.none.fl_str_mv 2015-12-15
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistas.ucc.edu.co/index.php/ml/article/view/1215
10.16925/cf.v3i1.1215
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/44314
url https://revistas.ucc.edu.co/index.php/ml/article/view/1215
https://hdl.handle.net/20.500.12494/44314
identifier_str_mv 10.16925/cf.v3i1.1215
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistas.ucc.edu.co/index.php/ml/article/view/1215/1414
dc.rights.none.fl_str_mv Derechos de autor 2015 Revista Colombia Forense
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Derechos de autor 2015 Revista Colombia Forense
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Cooperativa de Colombia / Instituto Nacional de Medicina Legal y Ciencias Forenses
dc.source.eng.fl_str_mv Colombia Forense; Vol. 2 No. 1; 45-56
dc.source.spa.fl_str_mv Colombia Forense; Vol. 2 Núm. 1; 45-56
dc.source.por.fl_str_mv Colombia Forense; v. 2 n. 1; 45-56
dc.source.none.fl_str_mv 2145-9649
2145-0684
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
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spelling Laverde-Angarita, Lilia JudithClavijo-Bolívar, Yolanda2015-12-152022-03-02T16:45:17Z2022-03-02T16:45:17Zhttps://revistas.ucc.edu.co/index.php/ml/article/view/121510.16925/cf.v3i1.1215https://hdl.handle.net/20.500.12494/44314Introduction: The laboratory receives different evidence for analysis, which maycontain fluids such as blood, semen, saliva or urine. A support tool in identifying nonvisible biological stains is observation with forensic lights. At present, there have been research advances in reference to wavelength and combination of different filters for the observation of biological fluids. Methodology: For this research, the alternate lights equipment Polilight® Flare with blue light was used, along with a filter and orange glasses, for observing stains of biological fluids deposited on different types of textiles and other surfaces that are frequently received by the laboratory. Results: The main result of this study was to determine the reliability of this equipment in terms of reproducibility andrepeatability of results, as well as the influence of variables such as substrate, age of the stain, source of fluid, and possible interferents in the observation of biological fluid fluorescence. Conclusions: It was found that biological stains emitting the strongest fluorescence were semen followed by urine and saliva stains. Bloodstains did not emit fluorescence and were referred to as non-fluorescent. Permeability of substrates made fluorescence halo greater, especially on light colors. Moreover, no statistically significant differences were observed in the fluorescence emission of azoospermic semen with respect to normspermic semen.Introducción: al laboratorio llegan diferentes soportes para ser analizados, los cuales pueden contener fluidos como sangre, semen, saliva u orina. Una herramienta de apoyo en la identificación de manchas biológicas no visibles es la observación con luces forenses. En la actualidad, se cuenta con avances investigativos en los que se hace referencia a la longitud de onda y a la combinación con diferentes filtros para la observación de fluidos biológicos. Metodología: en esta investigación, se utilizó el equipo de luces alternas Polilight® Flare con luz azul, junto con filtro y gafas naranjas, para la observación de manchas de fluidos biológicos depositados en distintos tipos de textiles y otras superficies que se reciben con frecuencia en el laboratorio. Resultados: el principal resultado de este estudio fue determinar la confiabilidad del equipo en términos de reproducibilidad y repetitividad de los resultados, al igual que la influencia de variables tales como el sustrato, la edad de la mancha, el origen del fluido y posibles interferentes en la observación de fluorescencia de fluidos biológicos. Conclusiones: se encontró que las manchas biológicas que emitieron la fluorescencia más fuerte fueron las de semen, seguidas de las manchas de orina y saliva. Las manchas de sangre no emitieron fluorescencia y fueron denominadas como no-fluorescentes. La permeabilidad de los sustratos hizo que el halo de fluorescencia fuera mayor sobre todo en los de colores claros. De otro lado, no se observaron diferencias estadísticamente significativas en la emisión de fluorescencia del semen azoospérmico con respecto al normospérmico.Introdução: diferentes suportes chegam ao laboratório para serem analisados,os quais podem conter fluídos como sangue, sêmen, saliva ou urina. Uma ferramenta de apoio na identificação de manchas biológicas não visíveis é a observação com luzes forenses. Na atualidade, conta-se com avanços investigativos nos quais se faz referência à longitude de onda e à combinação com diferentes filtros para a observação de fluídos biológicos. Metodologia: nesta pesquisa, utilizou-se o equipamento de luzes alternas Polilight® Flare com luz azul, junto com filtro e óculos cor-de-laranja para a observação de manchas de fluídos biológicos depositados em diferentes tipos de têxtis e outras superfícies que são recebidas com frequência no laboratório. Resultados: o principal resultado deste estudo foi determinar a confiabilidade do equipamento em termos de reprodutibilidade e repetitividade dos resultados, assim como a influência de variáveis como o substrato, a idade da mancha, a origem do fluído e possíveis interferentes na observação de fluorescência de fluídos biológicos. Conclusões: constatou-se que as manchas biológicas que emitiram a fluorescência mais forte foram as de sêmen; em seguida, as de urina esaliva. As manchas de sangue não emitiram fluorescência e foram denominadas como não fluorescentes. A permeabilidade dos substratos fez com que o halo de fluorescência fosse maior, principalmente, nos de cores claras. Por outro lado, não foram observadas diferenças estatisticamente significativas na emissão de fluorescência do sêmen azoospérmico a respeito do normospérmico.application/pdfspaUniversidad Cooperativa de Colombia / Instituto Nacional de Medicina Legal y Ciencias Forenseshttps://revistas.ucc.edu.co/index.php/ml/article/view/1215/1414Derechos de autor 2015 Revista Colombia Forenseinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Colombia Forense; Vol. 2 No. 1; 45-56Colombia Forense; Vol. 2 Núm. 1; 45-56Colombia Forense; v. 2 n. 1; 45-562145-96492145-0684forensic sciencesbiological fluidsforensic lightsevidenceciencias forensesfluidos biológicosluces forensessoportesciências forensesfluídos biológicosluzes forensessuportesInfluence of evidence, time, source and interferents in the observation of biological fluids with forensic lightsInfluencia de los soportes, tiempo, origen e interferentes en la observación de fluidos biológicos con luces forensesInfluência dos suportes, tempo, origem e interferentes na observação de fluídos biológicos com luzes forensesArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionPublication20.500.12494/44314oai:repository.ucc.edu.co:20.500.12494/443142024-07-16 13:30:32.571metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com