Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae)

We report the first studies of characterization and extraction of the Pamphobeteus aff. nigricolor (Pocock, 1901) (Aranae:Theraphosidae) venom done in Colombia using the electro-stimulation technique previous anesthesia with isofluorane. After each extraction process, a low viscosity, colorless veno...

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Autores:: Estrada Gomez, S.
Vargas Muñoz, Leidy Johana
Quintana Castillo, Juan Carlos

Tipo de recurso:: Article of journal

Fecha de publicación:: 2023

Institución:: Universidad Cooperativa de Colombia

Repositorio:: Repositorio UCC

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network_acronym_str	COOPER2
network_name_str	Repositorio UCC
repository_id_str
spelling	Estrada Gomez, S.Vargas Muñoz, Leidy JohanaQuintana Castillo, Juan Carlos2023-05-24T16:21:40Z2023-05-24T16:21:40Z01/01/2013https://doi.org/10.1016/j.toxicon.2013.10.014https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887689821&doi=10.1016%2fj.toxicon.2013.10.014&partnerID=40&md5=34e75b1e6440e80b01c819b136320eba00410101https://hdl.handle.net/20.500.12494/49720Estrada-Gomez S.,Vargas Muñoz L.J.,Quintana Castillo Juan carlos.Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae).Toxicon. 2013. 76.:p. 301-309We report the first studies of characterization and extraction of the Pamphobeteus aff. nigricolor (Pocock, 1901) (Aranae:Theraphosidae) venom done in Colombia using the electro-stimulation technique previous anesthesia with isofluorane. After each extraction process, a low viscosity, colorless venom was obtained. This venom showed a 1.01 mg/µl density and a pH of 5. The humidity percentage did not show a significance difference between males and females (P > 0.05) with a general media of 77.49 ± 1.74%. In all cases the venom yielded was variable between males and females, with a media of 22.45 ± 5.17 mg (wet weight) and 4.58 ± 0.94 mg (dry weigh), obtaining larger amounts in females, 28.34 ± 7.49 mg and 5.69 ± 1.36 (wet and dry weight respectively). Venom showed a hemolytic activity dependent of enzymatic active phospholipase and neither coagulant nor proteolytic activities were observed. Electrophoretic profile showed a main protein content with a molecular mass below 14 kDa. RP-HPLC venom profile revealed a difference among male and female venom's content where 17 and 21 main fractions were obtained respectively. Three peptides, Theraphotoxin-Pn1a, Theraphotoxin-Pn1b and Theraphotoxin-Pn2a, were identified using HPLC-nESI-MS/MS. These peptides showed a high identity with other peptides found on Theraphosides which are proved to affect voltage-gated calcium channels. © 2013 Elsevier Ltd. All rights reserved.0000-0002-7923-9158juan.quintanac@campusucc.edu.co301-309Elsevier LtdANIMAL TISSUEANIMALSARANEAEARTICLEBLOOD CLOTTINGCOLOMBIACHEMICAL FRACTIONATIONCHROMATOGRAPHY, HIGH PRESSURE LIQUIDCHROMATOGRAPHY, REVERSE-PHASEDRY WEIGHTELECTROPHORESISELECTROPHORESIS, POLYACRYLAMIDE GELELECTROSPRAY MASS SPECTROMETRYFEMALEHEMOLYSISHIGH PERFORMANCE LIQUID CHROMATOGRAPHYHUMIDITYMALENONHUMANPAMPHOBETEUS NIGRICOLORPEPTIDE ANALYSISPH MEASUREMENTPHOSPHOLIPASE A2PRIORITY JOURNALPROTEIN DEGRADATIONREVERSED PHASE LIQUID CHROMATOGRAPHYRP-HPLCSEX FACTORSSPIDER VENOMSPIDER VENOMSSPIDERSTARANTULATHERAPHOSIDAETHERAPHOTOXIN PN1ATHERAPHOTOXIN PN1BTHERAPHOTOXIN PN2ATOXINTOXIN ANALYSISUNCLASSIFIED DRUGVENOM EXTRACTIONVISCOSITYVOLTAGE GATED CALCIUM CHANNELExtraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae)Artículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionToxiconinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Publication20.500.12494/49720oai:repository.ucc.edu.co:20.500.12494/497202024-08-20 16:19:39.222metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv	Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae)
title	Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae)
spellingShingle	Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae) ANIMAL TISSUE ANIMALS ARANEAE ARTICLE BLOOD CLOTTING COLOMBIA CHEMICAL FRACTIONATION CHROMATOGRAPHY, HIGH PRESSURE LIQUID CHROMATOGRAPHY, REVERSE-PHASE DRY WEIGHT ELECTROPHORESIS ELECTROPHORESIS, POLYACRYLAMIDE GEL ELECTROSPRAY MASS SPECTROMETRY FEMALE HEMOLYSIS HIGH PERFORMANCE LIQUID CHROMATOGRAPHY HUMIDITY MALE NONHUMAN PAMPHOBETEUS NIGRICOLOR PEPTIDE ANALYSIS PH MEASUREMENT PHOSPHOLIPASE A2 PRIORITY JOURNAL PROTEIN DEGRADATION REVERSED PHASE LIQUID CHROMATOGRAPHY RP-HPLC SEX FACTORS SPIDER VENOM SPIDER VENOMS SPIDERS TARANTULA THERAPHOSIDAE THERAPHOTOXIN PN1A THERAPHOTOXIN PN1B THERAPHOTOXIN PN2A TOXIN TOXIN ANALYSIS UNCLASSIFIED DRUG VENOM EXTRACTION VISCOSITY VOLTAGE GATED CALCIUM CHANNEL
title_short	Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae)
title_full	Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae)
title_fullStr	Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae)
title_full_unstemmed	Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae)
title_sort	Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae)
dc.creator.fl_str_mv	Estrada Gomez, S. Vargas Muñoz, Leidy Johana Quintana Castillo, Juan Carlos
dc.contributor.author.none.fl_str_mv	Estrada Gomez, S. Vargas Muñoz, Leidy Johana Quintana Castillo, Juan Carlos
dc.subject.spa.fl_str_mv	ANIMAL TISSUE ANIMALS ARANEAE ARTICLE BLOOD CLOTTING COLOMBIA CHEMICAL FRACTIONATION CHROMATOGRAPHY, HIGH PRESSURE LIQUID CHROMATOGRAPHY, REVERSE-PHASE DRY WEIGHT ELECTROPHORESIS ELECTROPHORESIS, POLYACRYLAMIDE GEL ELECTROSPRAY MASS SPECTROMETRY FEMALE HEMOLYSIS HIGH PERFORMANCE LIQUID CHROMATOGRAPHY HUMIDITY MALE NONHUMAN PAMPHOBETEUS NIGRICOLOR PEPTIDE ANALYSIS PH MEASUREMENT PHOSPHOLIPASE A2 PRIORITY JOURNAL PROTEIN DEGRADATION REVERSED PHASE LIQUID CHROMATOGRAPHY RP-HPLC SEX FACTORS SPIDER VENOM SPIDER VENOMS SPIDERS TARANTULA THERAPHOSIDAE THERAPHOTOXIN PN1A THERAPHOTOXIN PN1B THERAPHOTOXIN PN2A TOXIN TOXIN ANALYSIS UNCLASSIFIED DRUG VENOM EXTRACTION VISCOSITY VOLTAGE GATED CALCIUM CHANNEL
topic	ANIMAL TISSUE ANIMALS ARANEAE ARTICLE BLOOD CLOTTING COLOMBIA CHEMICAL FRACTIONATION CHROMATOGRAPHY, HIGH PRESSURE LIQUID CHROMATOGRAPHY, REVERSE-PHASE DRY WEIGHT ELECTROPHORESIS ELECTROPHORESIS, POLYACRYLAMIDE GEL ELECTROSPRAY MASS SPECTROMETRY FEMALE HEMOLYSIS HIGH PERFORMANCE LIQUID CHROMATOGRAPHY HUMIDITY MALE NONHUMAN PAMPHOBETEUS NIGRICOLOR PEPTIDE ANALYSIS PH MEASUREMENT PHOSPHOLIPASE A2 PRIORITY JOURNAL PROTEIN DEGRADATION REVERSED PHASE LIQUID CHROMATOGRAPHY RP-HPLC SEX FACTORS SPIDER VENOM SPIDER VENOMS SPIDERS TARANTULA THERAPHOSIDAE THERAPHOTOXIN PN1A THERAPHOTOXIN PN1B THERAPHOTOXIN PN2A TOXIN TOXIN ANALYSIS UNCLASSIFIED DRUG VENOM EXTRACTION VISCOSITY VOLTAGE GATED CALCIUM CHANNEL
description	We report the first studies of characterization and extraction of the Pamphobeteus aff. nigricolor (Pocock, 1901) (Aranae:Theraphosidae) venom done in Colombia using the electro-stimulation technique previous anesthesia with isofluorane. After each extraction process, a low viscosity, colorless venom was obtained. This venom showed a 1.01 mg/µl density and a pH of 5. The humidity percentage did not show a significance difference between males and females (P > 0.05) with a general media of 77.49 ± 1.74%. In all cases the venom yielded was variable between males and females, with a media of 22.45 ± 5.17 mg (wet weight) and 4.58 ± 0.94 mg (dry weigh), obtaining larger amounts in females, 28.34 ± 7.49 mg and 5.69 ± 1.36 (wet and dry weight respectively). Venom showed a hemolytic activity dependent of enzymatic active phospholipase and neither coagulant nor proteolytic activities were observed. Electrophoretic profile showed a main protein content with a molecular mass below 14 kDa. RP-HPLC venom profile revealed a difference among male and female venom's content where 17 and 21 main fractions were obtained respectively. Three peptides, Theraphotoxin-Pn1a, Theraphotoxin-Pn1b and Theraphotoxin-Pn2a, were identified using HPLC-nESI-MS/MS. These peptides showed a high identity with other peptides found on Theraphosides which are proved to affect voltage-gated calcium channels. © 2013 Elsevier Ltd. All rights reserved.
publishDate	2023
dc.date.issued.none.fl_str_mv	01/01/2013
dc.date.accessioned.none.fl_str_mv	2023-05-24T16:21:40Z
dc.date.available.none.fl_str_mv	2023-05-24T16:21:40Z
dc.type.none.fl_str_mv	Artículo
dc.type.coar.fl_str_mv	http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv	http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv	info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv	http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv	info:eu-repo/semantics/publishedVersion
format	http://purl.org/coar/resource_type/c_6501
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://doi.org/10.1016/j.toxicon.2013.10.014 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887689821&doi=10.1016%2fj.toxicon.2013.10.014&partnerID=40&md5=34e75b1e6440e80b01c819b136320eba
dc.identifier.issn.spa.fl_str_mv	00410101
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/20.500.12494/49720
dc.identifier.bibliographicCitation.spa.fl_str_mv	Estrada-Gomez S.,Vargas Muñoz L.J.,Quintana Castillo Juan carlos.Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae).Toxicon. 2013. 76.:p. 301-309
url	https://doi.org/10.1016/j.toxicon.2013.10.014 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887689821&doi=10.1016%2fj.toxicon.2013.10.014&partnerID=40&md5=34e75b1e6440e80b01c819b136320eba https://hdl.handle.net/20.500.12494/49720
identifier_str_mv	00410101 Estrada-Gomez S.,Vargas Muñoz L.J.,Quintana Castillo Juan carlos.Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae).Toxicon. 2013. 76.:p. 301-309
dc.relation.ispartofjournal.spa.fl_str_mv	Toxicon
dc.rights.accessrights.none.fl_str_mv	info:eu-repo/semantics/openAccess
dc.rights.coar.none.fl_str_mv	http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
rights_invalid_str_mv	http://purl.org/coar/access_right/c_abf2
dc.format.extent.spa.fl_str_mv	301-309
dc.publisher.spa.fl_str_mv	Elsevier Ltd
institution	Universidad Cooperativa de Colombia
repository.name.fl_str_mv	Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv	bdigital@metabiblioteca.com
_version_	1814246979361832960

Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae)

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