Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae)

We report the first studies of characterization and extraction of the Pamphobeteus aff. nigricolor (Pocock, 1901) (Aranae:Theraphosidae) venom done in Colombia using the electro-stimulation technique previous anesthesia with isofluorane. After each extraction process, a low viscosity, colorless venom was obtained. This venom showed a 1.01 mg/µl density and a pH of 5. The humidity percentage did not show a significance difference between males and females (P > 0.05) with a general media of 77.49 ± 1.74%. In all cases the venom yielded was variable between males and females, with a media of 22.45 ± 5.17 mg (wet weight) and 4.58 ± 0.94 mg (dry weigh), obtaining larger amounts in females, 28.34 ± 7.49 mg and 5.69 ± 1.36 (wet and dry weight respectively). Venom showed a hemolytic activity dependent of enzymatic active phospholipase and neither coagulant nor proteolytic activities were observed. Electrophoretic profile showed a main protein content with a molecular mass below 14 kDa. RP-HPLC venom profile revealed a difference among male and female venom's content where 17 and 21 main fractions were obtained respectively. Three peptides, Theraphotoxin-Pn1a, Theraphotoxin-Pn1b and Theraphotoxin-Pn2a, were identified using HPLC-nESI-MS/MS. These peptides showed a high identity with other peptides found on Theraphosides which are proved to affect voltage-gated calcium channels. © 2013 Elsevier Ltd. All rights reserved.

• Autores: Estrada Gomez, S.; Vargas Muñoz, Leidy Johana; Quintana Castillo, Juan Carlos
• Tipo de recurso: Article of journal
• Fecha de publicación: 2023
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/49720
• Acceso en línea: https://doi.org/10.1016/j.toxicon.2013.10.014; https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887689821&doi=10.1016%2fj.toxicon.2013.10.014&partnerID=40&md5=34e75b1e6440e80b01c819b136320eba; https://hdl.handle.net/20.500.12494/49720
• Palabra clave: ANIMAL TISSUE; ANIMALS; ARANEAE; ARTICLE; BLOOD CLOTTING; COLOMBIA; CHEMICAL FRACTIONATION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; DRY WEIGHT; ELECTROPHORESIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ELECTROSPRAY MASS SPECTROMETRY; FEMALE; HEMOLYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMIDITY; MALE; NONHUMAN; PAMPHOBETEUS NIGRICOLOR; PEPTIDE ANALYSIS; PH MEASUREMENT; PHOSPHOLIPASE A2; PRIORITY JOURNAL; PROTEIN DEGRADATION; REVERSED PHASE LIQUID CHROMATOGRAPHY; RP-HPLC; SEX FACTORS; SPIDER VENOM; SPIDER VENOMS; SPIDERS; TARANTULA; THERAPHOSIDAE; THERAPHOTOXIN PN1A; THERAPHOTOXIN PN1B; THERAPHOTOXIN PN2A; TOXIN; TOXIN ANALYSIS; UNCLASSIFIED DRUG; VENOM EXTRACTION; VISCOSITY; VOLTAGE GATED CALCIUM CHANNEL
• Rights: openAccess
• License: http://purl.org/coar/access_right/c_abf2