In vitro evaluation of the osteogenic and antimicrobial potential of porous wollastonite scaffolds impregnated with ethanolic extracts of propolis
Context: The development of porous devices using materials modified with various natural agents has become a priority for bone healing processes in the oral and maxillofacial field. There must be a balance between the proliferation of eukaryotic and the inhibition of prokaryotic cells to achieve pro...
- Autores:
-
Moreno Florez, Ana Isabel
Malagon, Sarita
Ocampo, Sebastian
Leal Marín, Sara
Ossa, Edgar Alexander
Glasmacher, Birgit
García, Claudia
Peláez Vargas, Alejandro
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2024
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- eng
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/55108
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/55108
- Palabra clave:
- 610 - Medicina y salud
Scaffolds
Bone tissue engineering
wollastonite
3D printing
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/4.0/
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| dc.title.none.fl_str_mv |
In vitro evaluation of the osteogenic and antimicrobial potential of porous wollastonite scaffolds impregnated with ethanolic extracts of propolis |
| title |
In vitro evaluation of the osteogenic and antimicrobial potential of porous wollastonite scaffolds impregnated with ethanolic extracts of propolis |
| spellingShingle |
In vitro evaluation of the osteogenic and antimicrobial potential of porous wollastonite scaffolds impregnated with ethanolic extracts of propolis 610 - Medicina y salud Scaffolds Bone tissue engineering wollastonite 3D printing |
| title_short |
In vitro evaluation of the osteogenic and antimicrobial potential of porous wollastonite scaffolds impregnated with ethanolic extracts of propolis |
| title_full |
In vitro evaluation of the osteogenic and antimicrobial potential of porous wollastonite scaffolds impregnated with ethanolic extracts of propolis |
| title_fullStr |
In vitro evaluation of the osteogenic and antimicrobial potential of porous wollastonite scaffolds impregnated with ethanolic extracts of propolis |
| title_full_unstemmed |
In vitro evaluation of the osteogenic and antimicrobial potential of porous wollastonite scaffolds impregnated with ethanolic extracts of propolis |
| title_sort |
In vitro evaluation of the osteogenic and antimicrobial potential of porous wollastonite scaffolds impregnated with ethanolic extracts of propolis |
| dc.creator.fl_str_mv |
Moreno Florez, Ana Isabel Malagon, Sarita Ocampo, Sebastian Leal Marín, Sara Ossa, Edgar Alexander Glasmacher, Birgit García, Claudia Peláez Vargas, Alejandro |
| dc.contributor.author.none.fl_str_mv |
Moreno Florez, Ana Isabel Malagon, Sarita Ocampo, Sebastian Leal Marín, Sara Ossa, Edgar Alexander Glasmacher, Birgit García, Claudia Peláez Vargas, Alejandro |
| dc.contributor.researchgroup.none.fl_str_mv |
GIOM |
| dc.subject.ddc.none.fl_str_mv |
610 - Medicina y salud |
| topic |
610 - Medicina y salud Scaffolds Bone tissue engineering wollastonite 3D printing |
| dc.subject.proposal.none.fl_str_mv |
Scaffolds Bone tissue engineering wollastonite 3D printing |
| description |
Context: The development of porous devices using materials modified with various natural agents has become a priority for bone healing processes in the oral and maxillofacial field. There must be a balance between the proliferation of eukaryotic and the inhibition of prokaryotic cells to achieve proper bone health. Infections might inhibit the formation of new alveolar bone during bone graft augmentation. Objective: This study aimed to evaluate the in vitro osteogenic behavior of human bone marrow stem cells and assess the antimicrobial response to 3Dprinted porous scaffolds using propolis-modified wollastonite. Methodology: A fractional factorial design of experiments was used to obtain a 3D printing paste for developing scaffolds with a triply periodic minimal surface (TPMS) gyroid geometry based on wollastonite and modified with an ethanolic propolis extract. The antioxidant activity of the extracts was characterized using free radical scavenging methods (DPPH and ABTS). Cell proliferation and osteogenic potential using Human Bone Marrow Stem Cells (bmMSCs) were assessed at different culture time points up to 28 days. MIC and inhibition zones were studied from single strain cultures, and biofilm formation was evaluated on the scaffolds under co-culture conditions. The mechanical strength of the scaffolds was evaluated. Results: Through statistical design of experiments, a paste suitable for printing scaffolds with the desired geometry was obtained. Propolis extracts modifying the TPMS gyroid scaffolds showed favorable cell proliferation and metabolic activity with osteogenic potential after 21 days. Additionally, propolis exhibited antioxidant activity, which may be related to the antimicrobial effectiveness of the scaffolds against S. aureus and S. epidermidis cultures. The mechanical properties of the scaffolds were not affected by propolis impregnation. Conclusion: These results demonstrate that propolis-impregnated porous wollastonite scaffolds might have the potential to stimulate bone repair in maxillofacial tissue engineering applications. |
| publishDate |
2024 |
| dc.date.accessioned.none.fl_str_mv |
2024-03-01T19:17:24Z |
| dc.date.available.none.fl_str_mv |
2024-03-01T19:17:24Z |
| dc.date.issued.none.fl_str_mv |
2024-02-01 |
| dc.type.none.fl_str_mv |
Artículo |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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publishedVersion |
| dc.identifier.citation.none.fl_str_mv |
Florez AI, Malagon S, Ocampo S, Leal-Marin S, Ossa EA, Glasmacher B, Garcia C, Pelaez-Vargas A. (2024)In vitro evaluation of the osteogenic and antimicrobial potential of porous wollastonite scaffolds impregnated with ethanolic extracts of propolis. Frontiers in Bioengineering and Biotechnology. 2024;12.https://hdl.handle.net/20.500.12494/55108 |
| dc.identifier.issn.none.fl_str_mv |
2296-4185 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12494/55108 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fbioe.2024.1321466 |
| identifier_str_mv |
Florez AI, Malagon S, Ocampo S, Leal-Marin S, Ossa EA, Glasmacher B, Garcia C, Pelaez-Vargas A. (2024)In vitro evaluation of the osteogenic and antimicrobial potential of porous wollastonite scaffolds impregnated with ethanolic extracts of propolis. Frontiers in Bioengineering and Biotechnology. 2024;12.https://hdl.handle.net/20.500.12494/55108 2296-4185 10.3389/fbioe.2024.1321466 |
| url |
https://hdl.handle.net/20.500.12494/55108 |
| dc.language.iso.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.citationendpage.none.fl_str_mv |
12 p. |
| dc.relation.citationstartpage.none.fl_str_mv |
1 |
| dc.relation.ispartofjournal.none.fl_str_mv |
Frontiers in Bioengineering and Biotechnology |
| dc.relation.references.none.fl_str_mv |
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Cell Regen. 3, 8. doi:10.1186/2045-9769-3-8 |
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Moreno Florez, Ana IsabelMalagon, SaritaOcampo, SebastianLeal Marín, SaraOssa, Edgar AlexanderGlasmacher, BirgitGarcía, ClaudiaPeláez Vargas, AlejandroGIOM2024-03-01T19:17:24Z2024-03-01T19:17:24Z2024-02-01Florez AI, Malagon S, Ocampo S, Leal-Marin S, Ossa EA, Glasmacher B, Garcia C, Pelaez-Vargas A. (2024)In vitro evaluation of the osteogenic and antimicrobial potential of porous wollastonite scaffolds impregnated with ethanolic extracts of propolis. Frontiers in Bioengineering and Biotechnology. 2024;12.https://hdl.handle.net/20.500.12494/551082296-4185https://hdl.handle.net/20.500.12494/5510810.3389/fbioe.2024.1321466Context: The development of porous devices using materials modified with various natural agents has become a priority for bone healing processes in the oral and maxillofacial field. There must be a balance between the proliferation of eukaryotic and the inhibition of prokaryotic cells to achieve proper bone health. Infections might inhibit the formation of new alveolar bone during bone graft augmentation. Objective: This study aimed to evaluate the in vitro osteogenic behavior of human bone marrow stem cells and assess the antimicrobial response to 3Dprinted porous scaffolds using propolis-modified wollastonite. Methodology: A fractional factorial design of experiments was used to obtain a 3D printing paste for developing scaffolds with a triply periodic minimal surface (TPMS) gyroid geometry based on wollastonite and modified with an ethanolic propolis extract. The antioxidant activity of the extracts was characterized using free radical scavenging methods (DPPH and ABTS). Cell proliferation and osteogenic potential using Human Bone Marrow Stem Cells (bmMSCs) were assessed at different culture time points up to 28 days. MIC and inhibition zones were studied from single strain cultures, and biofilm formation was evaluated on the scaffolds under co-culture conditions. The mechanical strength of the scaffolds was evaluated. Results: Through statistical design of experiments, a paste suitable for printing scaffolds with the desired geometry was obtained. Propolis extracts modifying the TPMS gyroid scaffolds showed favorable cell proliferation and metabolic activity with osteogenic potential after 21 days. Additionally, propolis exhibited antioxidant activity, which may be related to the antimicrobial effectiveness of the scaffolds against S. aureus and S. epidermidis cultures. The mechanical properties of the scaffolds were not affected by propolis impregnation. Conclusion: These results demonstrate that propolis-impregnated porous wollastonite scaffolds might have the potential to stimulate bone repair in maxillofacial tissue engineering applications.Biomaterials12 p.application/pdfengSwitzerlandUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Odontología, Medellín y Envigadohttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://purl.org/coar/access_right/c_abf2610 - Medicina y saludScaffoldsBone tissue engineeringwollastonite3D printingIn vitro evaluation of the osteogenic and antimicrobial potential of porous wollastonite scaffolds impregnated with ethanolic extracts of propolisArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 p.1Frontiers in Bioengineering and BiotechnologyAbueidda, D., Elhebeary, M., Shiang, C., Pang, S., Al-Rub, R. 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