Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits
Background: Tomato mutants altered in leaf morphology are usually identified in the greenhouse, which demands considerable time and space and can only be performed in adequate periods. For a faster but equally reliable scrutiny method we addressed the screening in vitro of 971 T-DNA lines. Leaf deve...
- Autores:
-
Jáquez Gutiérrez, Marybel
Atarés, Alejandro
Pineda, Benito
Angarita Díaz, María del Pilar
Ribelles, Carlos
García Sogo, Begoña
Sánchez López, Jorge
Capel, Carmen
Yuste Lisbona, Fernando
Lozano, Rafael
Moreno, Vicente
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2019
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/17418
- Acceso en línea:
- https://doi.org/10.1186/s12870-019-1735-9
https://hdl.handle.net/20.500.12494/17418
- Palabra clave:
- Tomato
T-DNA lines
Screening in vitro
Mutants
Leaf development
Organ curvature
- Rights
- openAccess
- License
- Atribución
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| dc.title.spa.fl_str_mv |
Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits |
| title |
Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits |
| spellingShingle |
Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits Tomato T-DNA lines Screening in vitro Mutants Leaf development Organ curvature |
| title_short |
Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits |
| title_full |
Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits |
| title_fullStr |
Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits |
| title_full_unstemmed |
Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits |
| title_sort |
Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits |
| dc.creator.fl_str_mv |
Jáquez Gutiérrez, Marybel Atarés, Alejandro Pineda, Benito Angarita Díaz, María del Pilar Ribelles, Carlos García Sogo, Begoña Sánchez López, Jorge Capel, Carmen Yuste Lisbona, Fernando Lozano, Rafael Moreno, Vicente |
| dc.contributor.author.none.fl_str_mv |
Jáquez Gutiérrez, Marybel Atarés, Alejandro Pineda, Benito Angarita Díaz, María del Pilar Ribelles, Carlos García Sogo, Begoña Sánchez López, Jorge Capel, Carmen Yuste Lisbona, Fernando Lozano, Rafael Moreno, Vicente |
| dc.subject.spa.fl_str_mv |
Tomato T-DNA lines Screening in vitro Mutants Leaf development Organ curvature |
| topic |
Tomato T-DNA lines Screening in vitro Mutants Leaf development Organ curvature |
| description |
Background: Tomato mutants altered in leaf morphology are usually identified in the greenhouse, which demands considerable time and space and can only be performed in adequate periods. For a faster but equally reliable scrutiny method we addressed the screening in vitro of 971 T-DNA lines. Leaf development was evaluated in vitro in seedlings and shoot-derived axenic plants. New mutants were characterized in the greenhouse to establish the relationship between in vitro and in vivo leaf morphology, and to shed light on possible links between leaf development and agronomic traits, a promising field in which much remains to be discovered. Results: Following the screening in vitro of tomato T-DNA lines, putative mutants altered in leaf morphology were evaluated in the greenhouse. The comparison of results in both conditions indicated a general phenotypic correspondence, showing that in vitro culture is a reliable system for finding mutants altered in leaf development. Apart from providing homogeneous conditions, the main advantage of screening in vitro lies in the enormous time and space saving. Studies on the association between phenotype and nptII gene expression showed co-segregation in two lines (P > 99%). The use of an enhancer trap also allowed identifying gain-of-function mutants through reporter expression analysis. These studies suggested that genes altered in three other mutants were T-DNA tagged. New mutants putatively altered in brassinosteroid synthesis or perception, mutations determining multiple pleiotropic effects, lines affected in organ curvature, and the first tomato mutant with helical growth were discovered. Results also revealed new possible links between leaf development and agronomic traits, such as axillary branching, flower abscission, fruit development and fruit cracking. Furthermore, we found that the gene tagged in mutant 2635-MM encodes a Sterol 3-beta-glucosyltransferase. Expression analysis suggested that abnormal leaf development might be due to the lack-off-function of this gene. Conclusion: In vitro culture is a quick, efficient and reliable tool for identifying tomato mutants altered in leaf morphology. The characterization of new mutants in vivo revealed new links between leaf development and some agronomic traits. Moreover, the possible implication of a gene encoding a Sterol 3-beta-glucosyltransferase in tomato leaf development is reported. |
| publishDate |
2019 |
| dc.date.issued.none.fl_str_mv |
2019-04-15 |
| dc.date.accessioned.none.fl_str_mv |
2020-04-15T20:21:40Z |
| dc.date.available.none.fl_str_mv |
2020-04-15T20:21:40Z |
| dc.type.none.fl_str_mv |
Artículo |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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https://doi.org/10.1186/s12870-019-1735-9 |
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https://hdl.handle.net/20.500.12494/17418 |
| dc.identifier.bibliographicCitation.spa.fl_str_mv |
Jáquez Gutiérrez, M., Atarés, A., Pineda, B., Angarita, P., Ribelles, C., García Sogo, B. ... Moreno, V. (2019). Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits. BMC Plant Biol 19, 141. https://doi.org/10.1186/s12870-019-1735-9 |
| url |
https://doi.org/10.1186/s12870-019-1735-9 https://hdl.handle.net/20.500.12494/17418 |
| identifier_str_mv |
Jáquez Gutiérrez, M., Atarés, A., Pineda, B., Angarita, P., Ribelles, C., García Sogo, B. ... Moreno, V. (2019). Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits. BMC Plant Biol 19, 141. https://doi.org/10.1186/s12870-019-1735-9 |
| dc.relation.isversionof.spa.fl_str_mv |
https://link.springer.com/article/10.1186/s12870-019-1735-9 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
BMC Plant Biology |
| dc.relation.references.spa.fl_str_mv |
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1-24 |
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Akila Sridhar, BioMed Central, UK Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Odontología, Villavicencio |
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Odontología |
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Villavicencio |
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Universidad Cooperativa de Colombia |
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MD5 |
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Repositorio Institucional Universidad Cooperativa de Colombia |
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bdigital@metabiblioteca.com |
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1808789154623913984 |
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Jáquez Gutiérrez, MarybelAtarés, AlejandroPineda, BenitoAngarita Díaz, María del PilarRibelles, CarlosGarcía Sogo, BegoñaSánchez López, JorgeCapel, CarmenYuste Lisbona, FernandoLozano, RafaelMoreno, Vicente19/1412020-04-15T20:21:40Z2020-04-15T20:21:40Z2019-04-15https://doi.org/10.1186/s12870-019-1735-9https://hdl.handle.net/20.500.12494/17418Jáquez Gutiérrez, M., Atarés, A., Pineda, B., Angarita, P., Ribelles, C., García Sogo, B. ... Moreno, V. (2019). Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits. BMC Plant Biol 19, 141. https://doi.org/10.1186/s12870-019-1735-9Background: Tomato mutants altered in leaf morphology are usually identified in the greenhouse, which demands considerable time and space and can only be performed in adequate periods. For a faster but equally reliable scrutiny method we addressed the screening in vitro of 971 T-DNA lines. Leaf development was evaluated in vitro in seedlings and shoot-derived axenic plants. New mutants were characterized in the greenhouse to establish the relationship between in vitro and in vivo leaf morphology, and to shed light on possible links between leaf development and agronomic traits, a promising field in which much remains to be discovered. Results: Following the screening in vitro of tomato T-DNA lines, putative mutants altered in leaf morphology were evaluated in the greenhouse. The comparison of results in both conditions indicated a general phenotypic correspondence, showing that in vitro culture is a reliable system for finding mutants altered in leaf development. Apart from providing homogeneous conditions, the main advantage of screening in vitro lies in the enormous time and space saving. Studies on the association between phenotype and nptII gene expression showed co-segregation in two lines (P > 99%). The use of an enhancer trap also allowed identifying gain-of-function mutants through reporter expression analysis. These studies suggested that genes altered in three other mutants were T-DNA tagged. New mutants putatively altered in brassinosteroid synthesis or perception, mutations determining multiple pleiotropic effects, lines affected in organ curvature, and the first tomato mutant with helical growth were discovered. Results also revealed new possible links between leaf development and agronomic traits, such as axillary branching, flower abscission, fruit development and fruit cracking. Furthermore, we found that the gene tagged in mutant 2635-MM encodes a Sterol 3-beta-glucosyltransferase. Expression analysis suggested that abnormal leaf development might be due to the lack-off-function of this gene. Conclusion: In vitro culture is a quick, efficient and reliable tool for identifying tomato mutants altered in leaf morphology. 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Anal Biochem. 1999;15:41–9.TomatoT-DNA linesScreening in vitroMutantsLeaf developmentOrgan curvaturePhenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traitsArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAtribucióninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-84334https://repository.ucc.edu.co/bitstreams/a6100c51-1e71-44d7-8403-cf810906e4bb/download3bce4f7ab09dfc588f126e1e36e98a45MD5220.500.12494/17418oai:repository.ucc.edu.co:20.500.12494/174182024-08-10 22:41:16.345metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de 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