Evaluation of phenol-chloroform and silica columns methods for dna extraction from bone tissue
Purpose: To find factors for selecting genetic material extraction methods suchas phenol-chloroform and silica columns in order to use them in genetic analysis, in the framework of the policies of the Justice and Peace Act issued by the Government of Colombia. Description: A descri...
- Autores:
-
Alvis-Arango, Alveiro Antonio
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2015
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- spa
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/44313
- Acceso en línea:
- https://revistas.ucc.edu.co/index.php/ml/article/view/1199
https://hdl.handle.net/20.500.12494/44313
- Palabra clave:
- DNA
forensic genetics
method identification
bone tissue
ADN
genética forense
identificación de métodos
tejido óseo
DNA
genética forense
identificação de métodos
tecido ósseo
- Rights
- openAccess
- License
- Derechos de autor 2015 Revista Colombia Forense
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| dc.title.eng.fl_str_mv |
Evaluation of phenol-chloroform and silica columns methods for dna extraction from bone tissue |
| dc.title.spa.fl_str_mv |
Evaluación de los métodos fenol-cloroformo y columnas de sílice para extracción de ADN a partir de tejido óseo |
| dc.title.por.fl_str_mv |
Avaliação dos métodos fenol- clorofórmio e colunas de sílica para extração de dna a partir de tecido ósseo |
| title |
Evaluation of phenol-chloroform and silica columns methods for dna extraction from bone tissue |
| spellingShingle |
Evaluation of phenol-chloroform and silica columns methods for dna extraction from bone tissue DNA forensic genetics method identification bone tissue ADN genética forense identificación de métodos tejido óseo DNA genética forense identificação de métodos tecido ósseo |
| title_short |
Evaluation of phenol-chloroform and silica columns methods for dna extraction from bone tissue |
| title_full |
Evaluation of phenol-chloroform and silica columns methods for dna extraction from bone tissue |
| title_fullStr |
Evaluation of phenol-chloroform and silica columns methods for dna extraction from bone tissue |
| title_full_unstemmed |
Evaluation of phenol-chloroform and silica columns methods for dna extraction from bone tissue |
| title_sort |
Evaluation of phenol-chloroform and silica columns methods for dna extraction from bone tissue |
| dc.creator.fl_str_mv |
Alvis-Arango, Alveiro Antonio |
| dc.contributor.author.none.fl_str_mv |
Alvis-Arango, Alveiro Antonio |
| dc.subject.eng.fl_str_mv |
DNA forensic genetics method identification bone tissue |
| topic |
DNA forensic genetics method identification bone tissue ADN genética forense identificación de métodos tejido óseo DNA genética forense identificação de métodos tecido ósseo |
| dc.subject.spa.fl_str_mv |
ADN genética forense identificación de métodos tejido óseo |
| dc.subject.por.fl_str_mv |
DNA genética forense identificação de métodos tecido ósseo |
| description |
Purpose: To find factors for selecting genetic material extraction methods suchas phenol-chloroform and silica columns in order to use them in genetic analysis, in the framework of the policies of the Justice and Peace Act issued by the Government of Colombia. Description: A descriptive study was conducted using two main methods for dna extraction from bone tissue; test samples that initially did not yield reproducible and verifiable results were analyzed. Point of view: According to the results, it was found that the digestion time for bone samples subjected to dna extraction by the silica columns method improves when performed within two hours. Conclusions: dna extracted by the silica columnsmethod is better for recovery, but must be analyzed as soon as possible; otherwise, the material deteriorates when stored refrigerated. This phenomenon does not occur in extractions with phenol-chloroform. |
| publishDate |
2015 |
| dc.date.accessioned.none.fl_str_mv |
2022-03-02T16:45:17Z |
| dc.date.available.none.fl_str_mv |
2022-03-02T16:45:17Z |
| dc.date.none.fl_str_mv |
2015-12-15 |
| dc.type.none.fl_str_mv |
Artículo |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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http://purl.org/redcol/resource_type/ART |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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publishedVersion |
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https://revistas.ucc.edu.co/index.php/ml/article/view/1199 10.16925/cf.v3i1.1199 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12494/44313 |
| url |
https://revistas.ucc.edu.co/index.php/ml/article/view/1199 https://hdl.handle.net/20.500.12494/44313 |
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10.16925/cf.v3i1.1199 |
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spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistas.ucc.edu.co/index.php/ml/article/view/1199/1417 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2015 Revista Colombia Forense |
| dc.rights.accessrights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.coar.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| rights_invalid_str_mv |
Derechos de autor 2015 Revista Colombia Forense http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad Cooperativa de Colombia / Instituto Nacional de Medicina Legal y Ciencias Forenses |
| dc.source.eng.fl_str_mv |
Colombia Forense; Vol. 2 No. 1; 69-74 |
| dc.source.spa.fl_str_mv |
Colombia Forense; Vol. 2 Núm. 1; 69-74 |
| dc.source.por.fl_str_mv |
Colombia Forense; v. 2 n. 1; 69-74 |
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2145-9649 2145-0684 |
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Universidad Cooperativa de Colombia |
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Repositorio Institucional Universidad Cooperativa de Colombia |
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bdigital@metabiblioteca.com |
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1814247082195681280 |
| spelling |
Alvis-Arango, Alveiro Antonio2015-12-152022-03-02T16:45:17Z2022-03-02T16:45:17Zhttps://revistas.ucc.edu.co/index.php/ml/article/view/119910.16925/cf.v3i1.1199https://hdl.handle.net/20.500.12494/44313Purpose: To find factors for selecting genetic material extraction methods suchas phenol-chloroform and silica columns in order to use them in genetic analysis, in the framework of the policies of the Justice and Peace Act issued by the Government of Colombia. Description: A descriptive study was conducted using two main methods for dna extraction from bone tissue; test samples that initially did not yield reproducible and verifiable results were analyzed. Point of view: According to the results, it was found that the digestion time for bone samples subjected to dna extraction by the silica columns method improves when performed within two hours. Conclusions: dna extracted by the silica columnsmethod is better for recovery, but must be analyzed as soon as possible; otherwise, the material deteriorates when stored refrigerated. This phenomenon does not occur in extractions with phenol-chloroform.Propósito: encontrar factores para seleccionar métodos de extracción dematerial genético como el fenol-cloroformo y las columnas de sílice, a fin de utilizarlos en el análisis genético, en el marco de las políticas del Gobierno de Colombia de la Ley de Justicia y Paz. Descripción: se hizo un estudio descriptivo aplicando los dos principales métodos de extracción de adn óseo; se analizaron muestras problema que inicialmente no arrojaron resultados reproducibles y verificables. Punto de vista: según los resultados obtenidos, se halló que el tiempo de digestión para las muestras óseas sometidas a extracción de adn por el método de columnas de sílice mejora cuando se hace a las dos horas. Conclusiones: el adn extraído por el método de columnas de sílice es mejor en la recuperación, pero debe ser analizado lo más pronto posible; de lo contrario, el material se degrada si se conserva refrigerado. Este fenómeno no ocurre en extracciones con fenol-cloroformo. Propósito: encontrar fatores para selecionar métodos de extração de material genético como o fenol-clorofórmio e as colunas de sílica a fim de utilizá-los na análise genética, no âmbito das políticas do Governo da Colômbia da Lei de Justiça e Paz. Descrição: fez-se um estudo descritivo que aplicou os dois principais métodos de extração de dna ósseo; analisaram-se amostras-problema que inicialmente não deram resultados reproduzíveis e verificáveis. Ponto de vista: segundo os resultados obtidos, constatou-se que o tempo de digestão para as amostras ósseas submetidas à extração de dna pelo método de colunas de sílica melhora quando se faz às duas horas. Conclusões: o dna extraído pelo método de colunas de sílica é melhor na recuperação, mas deve ser analisado o mais breve possível; do contrário, o material se degrada se for conservado refrigerado. Esse fenômeno não ocorre em extrações com fenolclorofórmio.application/pdfspaUniversidad Cooperativa de Colombia / Instituto Nacional de Medicina Legal y Ciencias Forenseshttps://revistas.ucc.edu.co/index.php/ml/article/view/1199/1417Derechos de autor 2015 Revista Colombia Forenseinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Colombia Forense; Vol. 2 No. 1; 69-74Colombia Forense; Vol. 2 Núm. 1; 69-74Colombia Forense; v. 2 n. 1; 69-742145-96492145-0684DNAforensic geneticsmethod identificationbone tissueADNgenética forenseidentificación de métodostejido óseoDNAgenética forenseidentificação de métodostecido ósseoEvaluation of phenol-chloroform and silica columns methods for dna extraction from bone tissueEvaluación de los métodos fenol-cloroformo y columnas de sílice para extracción de ADN a partir de tejido óseoAvaliação dos métodos fenol- clorofórmio e colunas de sílica para extração de dna a partir de tecido ósseoArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionPublication20.500.12494/44313oai:repository.ucc.edu.co:20.500.12494/443132024-07-16 13:29:42.457metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com |