Evaluation of phenol-chloroform and silica columns methods for dna extraction from bone tissue

Purpose: To find factors for selecting genetic material extraction methods suchas phenol-chloroform and silica columns in order to use them in genetic analysis, in the framework of the policies of the Justice and Peace Act issued by the Government of Colombia. Description: A descriptive study was conducted using two main methods for dna extraction from bone tissue; test samples that initially did not yield reproducible and verifiable results were analyzed. Point of view: According to the results, it was found that the digestion time for bone samples subjected to dna extraction by the silica columns method improves when performed within two hours. Conclusions: dna extracted by the silica columnsmethod is better for recovery, but must be analyzed as soon as possible; otherwise, the material deteriorates when stored refrigerated. This phenomenon does not occur in extractions with phenol-chloroform.

• Autores: Alvis-Arango, Alveiro Antonio
• Tipo de recurso: Article of journal
• Fecha de publicación: 2015
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• Idioma: spa
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/44313
• Acceso en línea: https://revistas.ucc.edu.co/index.php/ml/article/view/1199; https://hdl.handle.net/20.500.12494/44313
• Palabra clave: DNA; forensic genetics; method identification; bone tissue; ADN; genética forense; identificación de métodos; tejido óseo; DNA; genética forense; identificação de métodos; tecido ósseo
• Rights: openAccess
• License: Derechos de autor 2015 Revista Colombia Forense