Simultaneous identification and qualitative determination of hydrocyanic acid and phosphine by gas chromatography with nitrogen-phosphorus detector and Headspace autosampler (hs-gc-npd) in biological fluids
Introduction: This article described a simple, rapid, sensitive methodology for the identification and qualitative determination of phosphine and hydrocyanic cyanide in biological (i.e., blood and stomach contents) and non-biological samples by gas chromatography (gc). Methodology: The foregoing was...
- Autores:
-
Monsalve-Salamanca, Luz Adriana
Ortiz-Rangel, María Martha
Mateus-Fontecha, Alexis
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2015
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- spa
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/44311
- Acceso en línea:
- https://revistas.ucc.edu.co/index.php/ml/article/view/1188
https://hdl.handle.net/20.500.12494/44311
- Palabra clave:
- cyanide
gas chromatography
phosphine
simultaneous identification
cianuro
cromatografía de gases
fosfina
identificación simultánea
cianeto
cromatografia gasosa
fosfina
identificação simultânea
- Rights
- openAccess
- License
- Derechos de autor 2015 Revista Colombia Forense
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| dc.title.eng.fl_str_mv |
Simultaneous identification and qualitative determination of hydrocyanic acid and phosphine by gas chromatography with nitrogen-phosphorus detector and Headspace autosampler (hs-gc-npd) in biological fluids |
| dc.title.spa.fl_str_mv |
Identificación simultánea y determinación cualitativa de ácido cianhídrico y fosfina por cromatografía de gases con detector de nitrógeno fósforo y automuestreador Headspace (hs-gc-npd) en fluidos biológicos |
| dc.title.por.fl_str_mv |
Identificação simultânea e determinação qualitativa de ácido cianídrico e fosfina por cromatografia gasosa com detector de nitrogênio fósforo e amostrador Headspace (hs-gc-npd) em fluídos biológicos |
| title |
Simultaneous identification and qualitative determination of hydrocyanic acid and phosphine by gas chromatography with nitrogen-phosphorus detector and Headspace autosampler (hs-gc-npd) in biological fluids |
| spellingShingle |
Simultaneous identification and qualitative determination of hydrocyanic acid and phosphine by gas chromatography with nitrogen-phosphorus detector and Headspace autosampler (hs-gc-npd) in biological fluids cyanide gas chromatography phosphine simultaneous identification cianuro cromatografía de gases fosfina identificación simultánea cianeto cromatografia gasosa fosfina identificação simultânea |
| title_short |
Simultaneous identification and qualitative determination of hydrocyanic acid and phosphine by gas chromatography with nitrogen-phosphorus detector and Headspace autosampler (hs-gc-npd) in biological fluids |
| title_full |
Simultaneous identification and qualitative determination of hydrocyanic acid and phosphine by gas chromatography with nitrogen-phosphorus detector and Headspace autosampler (hs-gc-npd) in biological fluids |
| title_fullStr |
Simultaneous identification and qualitative determination of hydrocyanic acid and phosphine by gas chromatography with nitrogen-phosphorus detector and Headspace autosampler (hs-gc-npd) in biological fluids |
| title_full_unstemmed |
Simultaneous identification and qualitative determination of hydrocyanic acid and phosphine by gas chromatography with nitrogen-phosphorus detector and Headspace autosampler (hs-gc-npd) in biological fluids |
| title_sort |
Simultaneous identification and qualitative determination of hydrocyanic acid and phosphine by gas chromatography with nitrogen-phosphorus detector and Headspace autosampler (hs-gc-npd) in biological fluids |
| dc.creator.fl_str_mv |
Monsalve-Salamanca, Luz Adriana Ortiz-Rangel, María Martha Mateus-Fontecha, Alexis |
| dc.contributor.author.none.fl_str_mv |
Monsalve-Salamanca, Luz Adriana Ortiz-Rangel, María Martha Mateus-Fontecha, Alexis |
| dc.subject.eng.fl_str_mv |
cyanide gas chromatography phosphine simultaneous identification |
| topic |
cyanide gas chromatography phosphine simultaneous identification cianuro cromatografía de gases fosfina identificación simultánea cianeto cromatografia gasosa fosfina identificação simultânea |
| dc.subject.spa.fl_str_mv |
cianuro cromatografía de gases fosfina identificación simultánea |
| dc.subject.por.fl_str_mv |
cianeto cromatografia gasosa fosfina identificação simultânea |
| description |
Introduction: This article described a simple, rapid, sensitive methodology for the identification and qualitative determination of phosphine and hydrocyanic cyanide in biological (i.e., blood and stomach contents) and non-biological samples by gas chromatography (gc). Methodology: The foregoing was carried out by a selective nitrogen-phosphorus detector (npd) and Headspace (hs) volatile autosampler using acetonitrile (acn) as internal standard (istd). The procedure involves taking and pouring into a vial 1 mL of the sample, which is added sulfuric acid. The vial is tightly sealed and subjected to incubation at 70 °C for 5 minutes. Finally, 1 mL of gas phase is injected into the gas chromatograph. Results: The limits of detection and quantification found for both phosphine and hydrocyanic acid were 0.04 mg/L and 0.2 mg/L, respectively. Conclusions: A chromatographic method was implemented and validated to allow simultaneous identification and quantitative determination of hydrocyanic acid and phosphine in biological samples such as blood and stomach contents. |
| publishDate |
2015 |
| dc.date.accessioned.none.fl_str_mv |
2022-03-02T16:45:17Z |
| dc.date.available.none.fl_str_mv |
2022-03-02T16:45:17Z |
| dc.date.none.fl_str_mv |
2015-12-15 |
| dc.type.none.fl_str_mv |
Artículo |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
| dc.type.coarversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.redcol.none.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
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info:eu-repo/semantics/publishedVersion |
| format |
http://purl.org/coar/resource_type/c_6501 |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistas.ucc.edu.co/index.php/ml/article/view/1188 10.16925/cf.v3i1.1188 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12494/44311 |
| url |
https://revistas.ucc.edu.co/index.php/ml/article/view/1188 https://hdl.handle.net/20.500.12494/44311 |
| identifier_str_mv |
10.16925/cf.v3i1.1188 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistas.ucc.edu.co/index.php/ml/article/view/1188/1412 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2015 Revista Colombia Forense |
| dc.rights.accessrights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
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http://purl.org/coar/access_right/c_abf2 |
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Derechos de autor 2015 Revista Colombia Forense http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad Cooperativa de Colombia / Instituto Nacional de Medicina Legal y Ciencias Forenses |
| dc.source.eng.fl_str_mv |
Colombia Forense; Vol. 2 No. 1; 21-26 |
| dc.source.spa.fl_str_mv |
Colombia Forense; Vol. 2 Núm. 1; 21-26 |
| dc.source.por.fl_str_mv |
Colombia Forense; v. 2 n. 1; 21-26 |
| dc.source.none.fl_str_mv |
2145-9649 2145-0684 |
| institution |
Universidad Cooperativa de Colombia |
| repository.name.fl_str_mv |
Repositorio Institucional Universidad Cooperativa de Colombia |
| repository.mail.fl_str_mv |
bdigital@metabiblioteca.com |
| _version_ |
1814246685905256448 |
| spelling |
Monsalve-Salamanca, Luz AdrianaOrtiz-Rangel, María MarthaMateus-Fontecha, Alexis2015-12-152022-03-02T16:45:17Z2022-03-02T16:45:17Zhttps://revistas.ucc.edu.co/index.php/ml/article/view/118810.16925/cf.v3i1.1188https://hdl.handle.net/20.500.12494/44311Introduction: This article described a simple, rapid, sensitive methodology for the identification and qualitative determination of phosphine and hydrocyanic cyanide in biological (i.e., blood and stomach contents) and non-biological samples by gas chromatography (gc). Methodology: The foregoing was carried out by a selective nitrogen-phosphorus detector (npd) and Headspace (hs) volatile autosampler using acetonitrile (acn) as internal standard (istd). The procedure involves taking and pouring into a vial 1 mL of the sample, which is added sulfuric acid. The vial is tightly sealed and subjected to incubation at 70 °C for 5 minutes. Finally, 1 mL of gas phase is injected into the gas chromatograph. Results: The limits of detection and quantification found for both phosphine and hydrocyanic acid were 0.04 mg/L and 0.2 mg/L, respectively. Conclusions: A chromatographic method was implemented and validated to allow simultaneous identification and quantitative determination of hydrocyanic acid and phosphine in biological samples such as blood and stomach contents.Introducción: en el presente artículo se describe una metodología sensible, rápida y sencilla para la identificación y determinación cualitativa de fosfina y ácido cianhídrico, en muestras biológicas (i.e., sangre y contenido gástrico) y no biológicas por cromatografía de gases (gc). Metodología: lo anterior se llevó a cabo por medio de un detector selectivo de nitrógeno-fósforo (npd) y automuestreador de volátiles Headspace (hs), empleando acetonitrilo (acn) como estándar interno (istd). El procedimiento consiste en tomar y servir en un vial 1mL de la muestra a la cual se le adiciona ácido sulfúrico; el vial se sella herméticamente y se somete a incubación a 70 °C por 5 minutos. Finalmente, se inyecta 1 mL de fase gaseosa al cromatógrafo de gases. Resultados: los límites de detección y cuantificación hallados, tanto para la fosfina, como para el ácido cianhídrico, fueron 0,04 mg/L y 0,2 mg/L, respectivamente. Conclusiones: se implementó y se validó un método cromatográfico que permite la identificación simultánea y la determinación cualitativa de ácido cianhídrico y fosfina en muestras biológicas como sangre y contenido gástrico. Introdução: neste artigo, descreve-se uma metodologia sensível, rápida e simples para a identificação e determinação qualitativa de fosfina e ácido cianídrico em amostras biológicas (por exemplo, sangue e conteúdo gástrico) e não biológicas por cromatografia gasosa (gc). Metodologia: isso foi realizado por meio de um detector seletivo de nitrogênio-fósforo (npd) e amostrador de voláteis Headspace (hs), que emprega acetonitrila (acn) como padrão interno (istd). O procedimento consiste em tomar e servir num tubo de vidro vial 1mL da amostra à qual é adicionado ácido sulfúrico; o vial é selado hermeticamente e submete-se à incubação a 70 °C por 5 minutos. Finalmente, injeta-se 1 mL de fase gasosa ao cromatógrafo a gás. Resultados: os limites de detecção e quantificação encontrados, tanto para a fosfina quanto para o ácido cianídrico, foram 0,04 mg/L e 0,2 mg/L, respectivamente. Conclusões: implementou-se e validou-se um método cromatográfico que permite a identificação simultânea e a determinação qualitativa de ácido cianídrico e fosfina em amostras biológicas como sangue e conteúdo gástrico.application/pdfspaUniversidad Cooperativa de Colombia / Instituto Nacional de Medicina Legal y Ciencias Forenseshttps://revistas.ucc.edu.co/index.php/ml/article/view/1188/1412Derechos de autor 2015 Revista Colombia Forenseinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Colombia Forense; Vol. 2 No. 1; 21-26Colombia Forense; Vol. 2 Núm. 1; 21-26Colombia Forense; v. 2 n. 1; 21-262145-96492145-0684cyanidegas chromatographyphosphinesimultaneous identificationcianurocromatografía de gasesfosfinaidentificación simultáneacianetocromatografia gasosafosfinaidentificação simultâneaSimultaneous identification and qualitative determination of hydrocyanic acid and phosphine by gas chromatography with nitrogen-phosphorus detector and Headspace autosampler (hs-gc-npd) in biological fluidsIdentificación simultánea y determinación cualitativa de ácido cianhídrico y fosfina por cromatografía de gases con detector de nitrógeno fósforo y automuestreador Headspace (hs-gc-npd) en fluidos biológicosIdentificação simultânea e determinação qualitativa de ácido cianídrico e fosfina por cromatografia gasosa com detector de nitrogênio fósforo e amostrador Headspace (hs-gc-npd) em fluídos biológicosArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionPublication20.500.12494/44311oai:repository.ucc.edu.co:20.500.12494/443112024-07-16 13:24:14.039metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com |