Simultaneous identification and qualitative determination of hydrocyanic acid and phosphine by gas chromatography with nitrogen-phosphorus detector and Headspace autosampler (hs-gc-npd) in biological fluids

Introduction: This article described a simple, rapid, sensitive methodology for the identification and qualitative determination of phosphine and hydrocyanic cyanide in biological (i.e., blood and stomach contents) and non-biological samples by gas chromatography (gc). Methodology: The foregoing was carried out by a selective nitrogen-phosphorus detector (npd) and Headspace (hs) volatile autosampler using acetonitrile (acn) as internal standard (istd). The procedure involves taking and pouring into a vial 1 mL of the sample, which is added sulfuric acid. The vial is tightly sealed and subjected to incubation at 70 °C for 5 minutes. Finally, 1 mL of gas phase is injected into the gas chromatograph. Results: The limits of detection and quantification found for both phosphine and hydrocyanic acid were 0.04 mg/L and 0.2 mg/L, respectively. Conclusions: A chromatographic method was implemented and validated to allow simultaneous identification and quantitative determination of hydrocyanic acid and phosphine in biological samples such as blood and stomach contents.

• Autores: Monsalve-Salamanca, Luz Adriana; Ortiz-Rangel, María Martha; Mateus-Fontecha, Alexis
• Tipo de recurso: Article of journal
• Fecha de publicación: 2015
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• Idioma: spa
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/44311
• Acceso en línea: https://revistas.ucc.edu.co/index.php/ml/article/view/1188; https://hdl.handle.net/20.500.12494/44311
• Palabra clave: cyanide; gas chromatography; phosphine; simultaneous identification; cianuro; cromatografía de gases; fosfina; identificación simultánea; cianeto; cromatografia gasosa; fosfina; identificação simultânea
• Rights: openAccess
• License: Derechos de autor 2015 Revista Colombia Forense