The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death
Strongyloidiasis is widely distributed in the tropical and subtropical areas. Ivermectin is the drug of choice for the treatment. However, the concerns about relying treatment on a single drug make identification of new molecules a priority. Alkylphospholipid analogues, including edelfosine, are a g...
- Autores:
-
Ceballos Legarda, Ana Lucya
Rojas Caraballo, José Vicente
López Aban, Julio
Ruano, Ana Lucia
Yepes Victoria, Edward
Gajate, Consuelo
Mollinedo, Faustino
Muro, Antonio
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2016
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/1002
- Acceso en línea:
- https://hdl.handle.net/20.500.12494/1002
- Palabra clave:
- Strongyloidiasis
Alkylphospholipid
Edelfosine
Apoptosis
- Rights
- openAccess
- License
- Licencia CC
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| dc.title.spa.fl_str_mv |
The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death |
| title |
The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death |
| spellingShingle |
The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death Strongyloidiasis Alkylphospholipid Edelfosine Apoptosis |
| title_short |
The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death |
| title_full |
The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death |
| title_fullStr |
The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death |
| title_full_unstemmed |
The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death |
| title_sort |
The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death |
| dc.creator.fl_str_mv |
Ceballos Legarda, Ana Lucya Rojas Caraballo, José Vicente López Aban, Julio Ruano, Ana Lucia Yepes Victoria, Edward Gajate, Consuelo Mollinedo, Faustino Muro, Antonio |
| dc.contributor.author.none.fl_str_mv |
Ceballos Legarda, Ana Lucya Rojas Caraballo, José Vicente López Aban, Julio Ruano, Ana Lucia Yepes Victoria, Edward Gajate, Consuelo Mollinedo, Faustino Muro, Antonio |
| dc.subject.spa.fl_str_mv |
Strongyloidiasis Alkylphospholipid Edelfosine Apoptosis |
| topic |
Strongyloidiasis Alkylphospholipid Edelfosine Apoptosis |
| description |
Strongyloidiasis is widely distributed in the tropical and subtropical areas. Ivermectin is the drug of choice for the treatment. However, the concerns about relying treatment on a single drug make identification of new molecules a priority. Alkylphospholipid analogues, including edelfosine, are a group of synthetic compounds that have shown activity against some parasites. The objective was to assess the in vitro and in vivo activity of edelfosine, miltefosine, perifosine against Strongyloides venezuelensis. Moreover, apoptosis-like mechanism in larvae after treatment was studied. Edelfosine displayed the highest activity and the best selectivity index (LD50=49.6 ± 5.4μM, SI=1.1) compared to miltefosine or perifosine. Third stage larvae after culture with edelfosine were not able to develop an infection in mice. Treatment of mice with edelfosine showed reduction of 47% in parasitic females allocated in the gut. Moreover, DNA fragmentation was observed by TUNEL staining in larvae treated with edelfosine. These results suggest that edelfosine could be an effective drug against strongyloidiasis, probably through induction of apoptosis-like cell death. |
| publishDate |
2016 |
| dc.date.issued.none.fl_str_mv |
2016-10 |
| dc.date.accessioned.none.fl_str_mv |
2017-08-10T01:06:56Z |
| dc.date.available.none.fl_str_mv |
2017-08-10T01:06:56Z |
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Artículo |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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publishedVersion |
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https://hdl.handle.net/20.500.12494/1002 |
| dc.identifier.bibliographicCitation.spa.fl_str_mv |
Legarda-Ceballo, AL., Rojas-Caraballo, J., Lopez-Aban, J., Ruano, AL., Yepes, E., Gajate, C., Mollinedo, F., Muro, A.. (2016, October). The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death. Acta Tropica , 162, 180-187 |
| url |
https://hdl.handle.net/20.500.12494/1002 |
| identifier_str_mv |
Legarda-Ceballo, AL., Rojas-Caraballo, J., Lopez-Aban, J., Ruano, AL., Yepes, E., Gajate, C., Mollinedo, F., Muro, A.. (2016, October). The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death. Acta Tropica , 162, 180-187 |
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http://www.sciencedirect.com/science/article/pii/S0001706X16304600?via%3Dihub |
| dc.rights.cc.none.fl_str_mv |
Licencia CC |
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info:eu-repo/semantics/openAccess |
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http://purl.org/coar/access_right/c_abf2 |
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Licencia CC http://purl.org/coar/access_right/c_abf2 |
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openAccess |
| dc.publisher.spa.fl_str_mv |
Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Santa Marta, Colombia |
| dc.publisher.program.spa.fl_str_mv |
Medicina |
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Santa Marta |
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Universidad Cooperativa de Colombia |
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Ceballos Legarda, Ana LucyaRojas Caraballo, José VicenteLópez Aban, Julio Ruano, Ana LuciaYepes Victoria, EdwardGajate, ConsueloMollinedo, FaustinoMuro, Antonio2017-08-10T01:06:56Z2017-08-10T01:06:56Z2016-10https://hdl.handle.net/20.500.12494/1002Legarda-Ceballo, AL., Rojas-Caraballo, J., Lopez-Aban, J., Ruano, AL., Yepes, E., Gajate, C., Mollinedo, F., Muro, A.. (2016, October). The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death. Acta Tropica , 162, 180-187Strongyloidiasis is widely distributed in the tropical and subtropical areas. Ivermectin is the drug of choice for the treatment. However, the concerns about relying treatment on a single drug make identification of new molecules a priority. Alkylphospholipid analogues, including edelfosine, are a group of synthetic compounds that have shown activity against some parasites. The objective was to assess the in vitro and in vivo activity of edelfosine, miltefosine, perifosine against Strongyloides venezuelensis. Moreover, apoptosis-like mechanism in larvae after treatment was studied. Edelfosine displayed the highest activity and the best selectivity index (LD50=49.6 ± 5.4μM, SI=1.1) compared to miltefosine or perifosine. Third stage larvae after culture with edelfosine were not able to develop an infection in mice. Treatment of mice with edelfosine showed reduction of 47% in parasitic females allocated in the gut. Moreover, DNA fragmentation was observed by TUNEL staining in larvae treated with edelfosine. These results suggest that edelfosine could be an effective drug against strongyloidiasis, probably through induction of apoptosis-like cell death.josev.rojas@campusucc.edu.coUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Santa Marta, ColombiaMedicinaSanta Martahttp://www.sciencedirect.com/science/article/pii/S0001706X16304600?via%3DihubStrongyloidiasisAlkylphospholipidEdelfosineApoptosisThe alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell deathArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionLicencia CCinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repository.ucc.edu.co/bitstreams/937c0b5c-69a1-4fc6-8fb4-04468bdca7f5/download8a4605be74aa9ea9d79846c1fba20a33MD52TEXT4.pdf.txt4.pdf.txtExtracted texttext/plain47568https://repository.ucc.edu.co/bitstreams/b0c6fdc0-d388-48f3-9f6c-5145fa195486/downloadb4c955e895d2736699922ead3f070a46MD53ORIGINAL4.pdf4.pdfapplication/pdf1216022https://repository.ucc.edu.co/bitstreams/1ee8666d-c6ac-4283-884c-1fa0c8ffd2c8/download98ba1762729461e2efbfb862a62beac4MD51THUMBNAIL4.pdf.jpg4.pdf.jpgIM Thumbnailimage/jpeg6220https://repository.ucc.edu.co/bitstreams/8a852e86-7753-4fa1-a715-8576ff0db9d5/downloadb60418946cad377cfb8761520ddba027MD5420.500.12494/1002oai:repository.ucc.edu.co:20.500.12494/10022024-08-10 22:45:06.76open.accesshttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.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 |