Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador
One of the most damaging diseases in cultivation of avocado (Persea americana Mill.) is root rot associated with Phytophthora cinnamomi Rands. This disease causes progressive wilt and even death of the tree. The objective of this study was to identify the presence of P. cinnamomi in two productive a...
- Autores:
-
Toapanta Gallegos, Diana Elizabeth
Morillo Velastegui, Luis Eduardo
Viera Arroyo, William Fernando
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2017
- Institución:
- Agrosavia
- Repositorio:
- Agrosavia
- Idioma:
- spa
- OAI Identifier:
- oai:repository.agrosavia.co:20.500.12324/34019
- Acceso en línea:
- http://revista.corpoica.org.co/index.php/revista/article/view/628
http://hdl.handle.net/20.500.12324/34019
- Palabra clave:
- Frutales
- Rights
- License
- Attribution-NonCommercial-ShareAlike 4.0 International
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Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador Diagnóstico molecular de Phytophthora cinnamomi asociado a la pudrición radicular en zonas productoras de aguacate en Ecuador Diagnóstico molecular de Phytophthora cinnamomi associado com a podridão radicular em áreas produtoras de abacate no Equador |
| title |
Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador |
| spellingShingle |
Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador Frutales |
| title_short |
Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador |
| title_full |
Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador |
| title_fullStr |
Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador |
| title_full_unstemmed |
Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador |
| title_sort |
Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador |
| dc.creator.fl_str_mv |
Toapanta Gallegos, Diana Elizabeth Morillo Velastegui, Luis Eduardo Viera Arroyo, William Fernando |
| dc.contributor.author.none.fl_str_mv |
Toapanta Gallegos, Diana Elizabeth Morillo Velastegui, Luis Eduardo Viera Arroyo, William Fernando |
| dc.subject.red.spa.fl_str_mv |
Frutales |
| topic |
Frutales |
| description |
One of the most damaging diseases in cultivation of avocado (Persea americana Mill.) is root rot associated with Phytophthora cinnamomi Rands. This disease causes progressive wilt and even death of the tree. The objective of this study was to identify the presence of P. cinnamomi in two productive areas of avocado in Ecuador using the molecular technique PCR-RFLP. Tree root samples were obtained with root rot symptoms in the production areas, from which 10 isolates were morphologically identified with Phytophthora spp. infection. To distinguish among the various Phytophthora species, a molecular analysis was performed using molecular markers in the ITSregion of the ribosomal DNA (rDNA). The ITSdigestion fragment obtained by PCR with the Ta qI enzyme confirmed the presence of Phytophthora cinnamomi in the isolated samples, and its association with root rot in the sampled production areas. |
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2017 |
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2017 |
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2018-11-06T20:41:44Z |
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2018-11-06T20:41:44Z |
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http://purl.org/coar/resource_type/c_6501 |
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article |
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http://revista.corpoica.org.co/index.php/revista/article/view/628 10.21930/rcta.vol18_num2_art:628 |
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http://hdl.handle.net/20.500.12324/34019 |
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reponame:Biblioteca Digital Agropecuaria de Colombia |
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http://revista.corpoica.org.co/index.php/revista/article/view/628 http://hdl.handle.net/20.500.12324/34019 |
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10.21930/rcta.vol18_num2_art:628 reponame:Biblioteca Digital Agropecuaria de Colombia repourl:https://repository.agrosavia.co instname:Corporación colombiana de investigación agropecuaria AGROSAVIA |
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spa |
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spa |
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http://revista.corpoica.org.co/index.php/revista/article/view/628/444 |
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Attribution-NonCommercial-ShareAlike 4.0 International |
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Corporación colombiana de investigación agropecuaria - AGROSAVIA |
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Revista Ciencia y Tecnología Agropecuaria; Vol 18 No 2 (2017); 285-294 |
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Toapanta Gallegos, Diana Elizabeth99fb0bd3-de4c-4449-8b47-67ec0ccea8dcMorillo Velastegui, Luis Eduardo92f356a5-bd9a-489f-a0f2-58cf0c521a58Viera Arroyo, William Fernando6da7c727-11a7-4ff3-a347-8793298368232018-11-06T20:41:44Z2018-11-06T20:41:44Z2017http://revista.corpoica.org.co/index.php/revista/article/view/62810.21930/rcta.vol18_num2_art:628http://hdl.handle.net/20.500.12324/34019reponame:Biblioteca Digital Agropecuaria de Colombiarepourl:https://repository.agrosavia.coinstname:Corporación colombiana de investigación agropecuaria AGROSAVIAOne of the most damaging diseases in cultivation of avocado (Persea americana Mill.) is root rot associated with Phytophthora cinnamomi Rands. This disease causes progressive wilt and even death of the tree. The objective of this study was to identify the presence of P. cinnamomi in two productive areas of avocado in Ecuador using the molecular technique PCR-RFLP. Tree root samples were obtained with root rot symptoms in the production areas, from which 10 isolates were morphologically identified with Phytophthora spp. infection. To distinguish among the various Phytophthora species, a molecular analysis was performed using molecular markers in the ITSregion of the ribosomal DNA (rDNA). The ITSdigestion fragment obtained by PCR with the Ta qI enzyme confirmed the presence of Phytophthora cinnamomi in the isolated samples, and its association with root rot in the sampled production areas. Phytophthora cinnamomi Rands está asociado con la pudrición radicular, una de las enfermedades más importantes en el cultivo de aguacate (Persea americana Mill.) debido a que ocasiona marchitez progresiva hasta provocar la muerte del árbol. El objetivo de este estudio fue identificar molecularmente la presencia de P. cinnamomi con la técnica PCR-RFLP, en plantaciones de aguacate en dos zonas productoras del Ecuador. Con este fin, se obtuvieron muestras de raíces de árboles con sintomatología de pudrición radicular en las zonas Palabras clave: aislamiento, Ecuador, Persea americana, Phytophthora cinnamomi de producción. A partir de estas se obtuvieron diez aislamientos donde se identificó morfológicamente a Phytophthora sp. Para la distinción de especies de Phytophthora se realizó un análisis molecular con marcadores en la región ITS de ADN ribosomal (ADNr). La digestión del fragmento ITS obtenido por PCR con la enzima TaqI permitió confirmar la presencia de P. cinnamomi en los aislamientos y, por lo tanto, asociar su presencia con la pudrición radicular en las zonas de producción muestreadas.Phytophthora cinnamomi Rands está associado com a podridão radicular, uma das doenças mais importantes no cultivo de abacate (Persea americanaMill.) devido a que ocasiona murchidão progressiva até provocar a morte da árvore. O objetivo deste estudo foi identificar molecularmente a presença de P. cinnamomi com a técnica PCR-RFLP, em plantações de abacate em duas áreas produtoras do Equador. Com esse propósito, obtiveram-se amostras de raízes de árvores com sintomatologia de podridão radicular nas áreas de produção. A partir destas, obtiveram-se dez isolamentos nos quais se identificou morfologicamente a Phytophthora sp. Para a distinção de espécies de Phytophthora, foi realizada uma análise molecular com marcadores na região ITS de DNAribossômico (DNAr). A digestão do fragmento ITS obtido por PCR com a enzima Ta qI permitiu confirmar a presença de P. cinnamomi nos isolamentos e, portanto, associar sua presença com a podridão radicular nas áreas de produção das amostras.Aguacate-Persea americanaapplication/pdfspaCorporación colombiana de investigación agropecuaria - AGROSAVIAhttp://revista.corpoica.org.co/index.php/revista/article/view/628/444Attribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/http://purl.org/coar/access_right/c_abf2Revista Ciencia y Tecnología Agropecuaria; Vol 18 No 2 (2017); 285-294Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of EcuadorDiagnóstico molecular de Phytophthora cinnamomi asociado a la pudrición radicular en zonas productoras de aguacate en EcuadorDiagnóstico molecular de Phytophthora cinnamomi associado com a podridão radicular em áreas produtoras de abacate no EquadorFrutalesarticleArtículo científicohttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articlehttps://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85ColombiaORIGINAL628-Texto del artículo-1777-2-10-20170508.pdf628-Texto del artículo-1777-2-10-20170508.pdfapplication/pdf252460https://repository.agrosavia.co/bitstream/20.500.12324/34019/1/628-Texto%20del%20art%c3%adculo-1777-2-10-20170508.pdff1fdb62adcf82dcfa34a51104c7eeea0MD51open accessTHUMBNAIL628-Texto del artículo-1777-2-10-20170508.pdf.jpg628-Texto del artículo-1777-2-10-20170508.pdf.jpgIM Thumbnailimage/jpeg9151https://repository.agrosavia.co/bitstream/20.500.12324/34019/2/628-Texto%20del%20art%c3%adculo-1777-2-10-20170508.pdf.jpg7f6163247d226ac65d22122e89ce30b2MD52open access20.500.12324/34019oai:repository.agrosavia.co:20.500.12324/340192023-05-04 22:00:48.225open accessAgrosavia - Corporación colombiana de investigación agropecuariabac@agrosavia.co |