Ensayo biológico y metodología estadística para estimar la potencia de un antibiótico
In this paper the standarization of the gel diffusion technique used to determine the potency of an antibiotic in relation to an international standard, and the statistical methods applied to biological assays involving a dose - response diagram in the quality control of these type of medicaments ar...
- Autores:
-
Moreno M., Gloria Stella
De Gerardino, Astrid G.
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 1989
- Institución:
- Agrosavia
- Repositorio:
- Agrosavia
- Idioma:
- spa
- OAI Identifier:
- oai:repository.agrosavia.co:20.500.12324/35326
- Acceso en línea:
- http://hdl.handle.net/20.500.12324/35326
- Palabra clave:
- Genética y mejoramiento animal - L10
Penicilina
Ampicilina
Experimentación
Métodos estadísticos
Control de calidad
Ganadería y especies menores
- Rights
- License
- Attribution-NonCommercial-NoDerivatives 4.0 International
| Summary: | In this paper the standarization of the gel diffusion technique used to determine the potency of an antibiotic in relation to an international standard, and the statistical methods applied to biological assays involving a dose - response diagram in the quality control of these type of medicaments are described. Sodium ampicillin was used as test - antibiotic in a "6 point assay" in a randomized block design with a factorial structure 2 x 3. The results were analyzed by analysis of variance and five orthogonal comparisons were stablished to test the validity of the assay. The estimation of the potency led to the conclusion that 100 ug of the test preparation is the equivalent of 89.35 ug of the standard and the true potency is very likely to lie between 97.87 ug and 81.46 ug ( c = 0.05) |
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